##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1432019
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85337485047335	31.0	31.0	34.0	30.0	34.0
2	31.96060666792829	33.0	31.0	34.0	30.0	34.0
3	31.97676497309044	33.0	31.0	34.0	30.0	34.0
4	35.61324814824384	37.0	35.0	37.0	33.0	37.0
5	35.458718773982746	37.0	35.0	37.0	33.0	37.0
6	35.40555118332927	37.0	35.0	37.0	33.0	37.0
7	35.702580761847436	37.0	35.0	37.0	35.0	37.0
8	35.59162762505246	37.0	35.0	37.0	35.0	37.0
9	37.35424460150319	39.0	37.0	39.0	34.0	39.0
10	36.740642407677555	39.0	37.0	39.0	32.0	39.0
11	36.646237933993895	39.0	35.0	39.0	32.0	39.0
12	36.551061124189	39.0	35.0	39.0	32.0	39.0
13	36.45274608786615	39.0	35.0	39.0	32.0	39.0
14	37.50759591876923	39.0	36.0	41.0	32.0	41.0
15	37.658134424194095	40.0	36.0	41.0	33.0	41.0
16	37.696365062195405	40.0	36.0	41.0	33.0	41.0
17	37.64335389404749	39.0	36.0	41.0	33.0	41.0
18	37.6182438920154	39.0	36.0	41.0	33.0	41.0
19	37.560746051553785	39.0	36.0	41.0	32.0	41.0
20	37.466946318449686	39.0	36.0	41.0	32.0	41.0
21	37.354081894164814	39.0	35.0	41.0	32.0	41.0
22	37.35204770327768	39.0	35.0	41.0	32.0	41.0
23	36.84436100359004	39.0	35.0	41.0	31.0	41.0
24	36.938412130006654	39.0	35.0	41.0	31.0	41.0
25	36.93732275898574	39.0	35.0	41.0	31.0	41.0
26	36.945594995597126	39.0	35.0	41.0	31.0	41.0
27	34.49273508242558	39.0	35.0	40.0	23.0	41.0
28	35.370796057873534	38.0	35.0	40.0	22.0	41.0
29	36.07237962624797	38.0	35.0	40.0	28.0	41.0
30	36.34371401496768	38.0	35.0	40.0	30.0	41.0
31	36.42251674035051	38.0	35.0	40.0	30.0	41.0
32	36.21175277702321	38.0	35.0	40.0	30.0	41.0
33	36.19711121151325	38.0	35.0	40.0	30.0	41.0
34	36.15678562924095	38.0	35.0	40.0	30.0	41.0
35	36.057069075200815	38.0	35.0	40.0	29.0	41.0
36	35.92625097851355	38.0	35.0	40.0	29.0	41.0
37	35.872746800147205	38.0	35.0	40.0	29.0	41.0
38	35.730944212332375	38.0	35.0	40.0	28.0	41.0
39	35.612190201386994	38.0	34.0	40.0	28.0	41.0
40	35.51107701783286	38.0	34.0	40.0	27.0	41.0
41	35.389087016303556	38.0	34.0	40.0	27.0	41.0
42	35.2968193857763	38.0	34.0	40.0	27.0	41.0
43	35.20281714139268	38.0	34.0	40.0	26.0	41.0
44	35.05156844985995	38.0	34.0	40.0	26.0	41.0
45	34.965802129720345	37.0	34.0	40.0	26.0	41.0
46	34.8320769486997	37.0	33.0	40.0	25.0	41.0
47	34.70644034750936	37.0	33.0	40.0	25.0	41.0
48	34.61262106159206	37.0	33.0	40.0	24.0	41.0
49	34.550280408290675	37.0	33.0	40.0	24.0	41.0
50	34.45169442584211	37.0	33.0	40.0	24.0	41.0
51	32.88395754525603	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	8.0
11	18.0
12	21.0
13	27.0
14	55.0
15	115.0
16	223.0
17	457.0
18	856.0
19	1515.0
20	2497.0
21	3550.0
22	5159.0
23	7299.0
24	9579.0
25	12988.0
26	16476.0
27	19865.0
28	24085.0
29	28992.0
30	35284.0
31	43376.0
32	55032.0
33	72737.0
34	107065.0
35	124616.0
36	135942.0
37	181610.0
38	250816.0
39	291694.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39988645402051	18.114494290927706	22.268908443253896	14.216710811797888
2	30.909645751906922	25.013983753008862	26.290433297323567	17.785937197760642
3	33.91672875848714	24.471253523870843	25.513977119018673	16.098040598623342
4	28.397528245086136	26.391479442661026	25.86830202671892	19.34269028553392
5	24.41657547839798	32.01954722667786	24.13620210346371	19.427675191460448
6	25.081999610340368	35.933182450791506	24.63437985110533	14.350438087762802
7	82.00491753251877	6.742857462086746	7.190477221321784	4.061747784072697
8	83.15727654451513	5.309566423350528	7.503671389834911	4.029485642299439
9	77.71530964323797	8.02880408709661	9.033748853890906	5.222137415774512
10	40.59254800390219	25.422707380279174	17.632796771551217	16.35194784426743
11	31.836169771490464	27.111721283027666	22.10312851994282	18.948980425539048
12	27.352849368618713	23.799963547969686	28.62853076670072	20.21865631671088
13	25.0511340980811	25.440723901009694	29.44625734714414	20.06188465376507
14	22.48650332153414	26.494620532269476	29.42998661330611	21.588889532890278
15	22.21325275712124	25.973887217976856	30.97829009251972	20.834569932382184
16	25.10169208648768	24.046957477519502	29.10163901456615	21.74971142142667
17	24.577606861361474	24.322721975057593	27.682593596872668	23.417077566708265
18	25.538138809610768	23.782575510520463	28.451926964656195	22.227358715212578
19	25.92703029778236	25.818232858642236	25.837436514459654	22.417300329115744
20	26.056637516680993	25.522007738724138	26.6712941657897	21.75006057880517
21	26.217668899644487	26.444062543862895	26.557818017777695	20.78045053871492
22	24.87264484619268	24.322093491776297	26.52960610159502	24.275655560436
23	23.20541836386249	27.516953336512994	26.188549174277714	23.0890791253468
24	22.876093124462734	25.611950679425345	28.41875701369884	23.09319918241308
25	24.321814165873498	26.48645024961261	25.87765944446268	23.314076140051217
26	22.68999224172305	26.701950183621864	26.644897867975214	23.96315970667987
27	21.847335824454845	30.67689744339984	25.054695503341783	22.42107122880353
28	22.502355066517975	26.522203965170853	27.41129831377936	23.56414265453182
29	23.28677203305263	26.626532189866197	26.031218859526305	24.055476917554866
30	24.144162891693476	25.58597337046506	26.98462799725423	23.28523574058724
31	25.63499506640624	26.61382286128885	24.454563801178615	23.29661827112629
32	25.05336870530349	26.083941623679575	25.213631942034286	23.649057728982644
33	24.490596842639658	26.65739770212546	24.69799632546775	24.154009129767132
34	23.957573188623893	26.354398929064487	26.157753493494152	23.530274388817467
35	24.38550047171162	26.783513347239108	25.152459569321355	23.678526611727918
36	24.15868783863901	28.38586638864429	24.80707309051067	22.648372682206034
37	25.012587123494868	28.35081098784304	23.88997632014659	22.7466255685155
38	23.72175229518603	28.1765116244966	25.827171287531797	22.27456479278557
39	24.51741212930834	26.387918037400343	25.537789652232266	23.55688018105905
40	25.11000203209594	25.732968626812912	27.381829431034088	21.775199910057058
41	22.72204488906921	26.927855007510377	25.53255229155479	24.817547811865627
42	22.99103573346443	27.04768581981105	26.133591802902057	23.827686643822464
43	23.301715968852367	25.581992976350175	26.930648266538366	24.18564278825909
44	24.0237734275872	25.212305143995994	26.8087225099667	23.955198918450105
45	24.048982590314793	25.15127243423446	26.2149454720922	24.584799503358546
46	24.026008034809593	25.915368441340515	26.38184269901447	23.676780824835426
47	22.632590768697902	27.503056872848752	26.585261787727678	23.279090570725668
48	22.558499572980526	26.52045817827836	27.228130353019058	23.692911895722055
49	23.374689860958547	26.04846723402413	26.975270579510468	23.601572325506854
50	22.387621951943377	25.918580689222697	27.56388008818319	24.12991727065074
51	22.874277506094543	25.423056537657672	26.1464407944308	25.556225161816986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3315.0
1	3466.0
2	3617.0
3	5104.5
4	6592.0
5	4933.0
6	3274.0
7	3743.5
8	4213.0
9	5405.5
10	6598.0
11	5373.0
12	4148.0
13	4144.0
14	4140.0
15	4075.0
16	4010.0
17	4111.0
18	4212.0
19	4181.5
20	4151.0
21	4803.5
22	5456.0
23	5714.0
24	5972.0
25	7050.0
26	9669.0
27	11210.0
28	14277.5
29	17345.0
30	19416.5
31	21488.0
32	24289.0
33	27090.0
34	31901.0
35	36712.0
36	40021.5
37	43331.0
38	45311.0
39	47291.0
40	52345.0
41	57399.0
42	63159.0
43	68919.0
44	74720.5
45	80522.0
46	89616.5
47	98711.0
48	105819.0
49	112927.0
50	113283.5
51	113640.0
52	105203.5
53	96767.0
54	90398.0
55	84029.0
56	79934.5
57	75840.0
58	71562.5
59	67285.0
60	65546.5
61	63808.0
62	59160.5
63	54513.0
64	49557.0
65	44601.0
66	39775.5
67	34950.0
68	31541.5
69	28133.0
70	26327.5
71	24522.0
72	21736.5
73	18951.0
74	15855.5
75	10752.5
76	8745.0
77	7257.0
78	5769.0
79	4452.5
80	3136.0
81	2495.0
82	1854.0
83	1528.5
84	1203.0
85	818.5
86	434.0
87	298.5
88	163.0
89	118.0
90	73.0
91	60.0
92	47.0
93	28.5
94	10.0
95	10.0
96	10.0
97	7.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1432019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.081301804998642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74650929031452	21.27060588056456
2	9.040560630853278	5.077414231227597
3	3.620228329727542	3.049821729902558
4	1.8798708854836483	2.111568867587855
5	1.1251102765688377	1.579728062011751
6	0.7714914513201222	1.299869057069806
7	0.6009734497386349	1.181328177323125
8	0.4663541467279213	1.0476665233823501
9	0.39005705309455074	0.9857978846204815
>10	5.054280310537562	34.69754934937656
>50	1.2026694265782878	22.240544864153463
>100	0.09816689241343497	4.224031585058766
>500	0.0024852377611553483	0.5565649752347553
>1k	0.0012426188805776742	0.6775088124863955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2781	0.19420133392084882	No Hit
ATATATATATGTATATATATACATATATATGTATATATATACATATATATG	2056	0.14357351403857072	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2011	0.1404310976320845	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1853	0.12939772447153286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0949442709908178E-4	0.0	0.0	0.075976645561267	0.0
2	2.0949442709908178E-4	0.0	0.0	0.3681515398887864	0.0
3	2.0949442709908178E-4	0.0	0.0	0.5142389870525461	0.0
4	2.0949442709908178E-4	0.0	0.0	0.7012476789763264	0.0
5	2.0949442709908178E-4	0.0	0.0	1.2741451056166153	0.0
6	2.0949442709908178E-4	0.0	0.0	1.8520005670315827	0.0
7	2.0949442709908178E-4	0.0	0.0	2.311910665989767	0.0
8	2.0949442709908178E-4	0.0	0.0	3.1132268496437545	0.0
9	2.0949442709908178E-4	0.0	0.0	3.501070865679855	0.0
10	2.0949442709908178E-4	0.0	0.0	4.281786764002433	0.0
11	2.0949442709908178E-4	0.0	0.0	5.138828465264776	0.0
12	2.0949442709908178E-4	0.0	0.0	5.866053453201389	0.0
13	2.0949442709908178E-4	0.0	0.0	6.140560984176886	0.0
14	2.793259027987757E-4	0.0	0.0	6.261858257467254	0.0
15	2.793259027987757E-4	0.0	0.0	6.459551165173088	0.0
16	2.793259027987757E-4	0.0	0.0	6.866947994405102	0.0
17	2.793259027987757E-4	0.0	0.0	7.303045560149691	0.0
18	2.793259027987757E-4	0.0	0.0	7.849337194548396	0.0
19	2.793259027987757E-4	0.0	0.0	8.114138150401637	0.0
20	2.793259027987757E-4	0.0	0.0	8.401773998808675	0.0
21	2.793259027987757E-4	0.0	0.0	8.71804075225259	0.0
22	3.4915737849846966E-4	0.0	0.0	9.053720655941017	0.0
23	3.4915737849846966E-4	0.0	0.0	9.412724272513143	0.0
24	3.4915737849846966E-4	0.0	0.0	9.700010963541684	0.0
25	3.4915737849846966E-4	0.0	0.0	9.953289725904474	0.0
26	3.4915737849846966E-4	0.0	0.0	10.186876012119951	0.0
27	4.1898885419816356E-4	0.0	0.0	10.436383874794958	0.0
28	4.1898885419816356E-4	0.0	0.0	10.712148372333049	0.0
29	4.1898885419816356E-4	0.0	0.0	11.002996468622273	0.0
30	4.1898885419816356E-4	0.0	0.0	11.332042382119232	0.0
31	4.1898885419816356E-4	0.0	0.0	11.62428710792245	0.0
32	4.1898885419816356E-4	0.0	0.0	11.936643298727182	0.0
33	4.1898885419816356E-4	0.0	0.0	12.241597353107744	0.0
34	4.1898885419816356E-4	0.0	0.0	12.542152024519227	0.0
35	4.1898885419816356E-4	0.0	0.0	12.855625518935154	0.0
36	4.1898885419816356E-4	0.0	0.0	13.1419345693039	0.0
37	4.1898885419816356E-4	0.0	0.0	13.45177682698344	0.0
38	4.1898885419816356E-4	0.0	0.0	13.788643865758765	0.0
39	4.888203298978575E-4	0.0	0.0	14.18647378281992	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.033868E-4	45.000004	37
CGTTCAT	75	0.0	42.0	17
ACACGAC	450	0.0	41.5	26
AACACGT	495	0.0	40.454544	41
TCGCGAA	45	1.9288564E-8	40.0	15
TACGGGT	160	0.0	39.375004	4
CACGACC	480	0.0	38.906254	27
ATAACGC	110	0.0	38.863636	11
TCAAGCG	505	0.0	38.762375	17
TTAACGC	35	6.2493655E-6	38.571426	14
GACACGA	485	0.0	38.041237	25
GTTAGCG	95	0.0	37.894737	1
GCTACGA	155	0.0	37.741936	10
ACGACCA	525	0.0	37.714283	28
TGCGATA	30	1.1400618E-4	37.500004	44
TTTAGCG	115	0.0	37.173916	1
TGGTCAA	515	0.0	37.13592	14
CGTAAGG	285	0.0	37.105263	2
ACACGTG	545	0.0	36.74312	42
GCGATAT	135	0.0	36.666664	9
>>END_MODULE