FastQCFastQC Report
Sat 18 Jun 2016
SRR3553991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584153
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.5056329786327457No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC19870.12542980381314178TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19400.12246291867010321No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT16100.1016315974530238TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG453.8562575E-1045.01
CGTTTTT58400.041.609591
CGTAAGG1650.039.5454522
GCTACGA2450.039.48979610
CTATACG403.4593177E-739.3750041
CGTTCGA800.039.37500414
CATAACG403.4593177E-739.3750041
GCGTATG1100.038.8636321
TGTTACG1200.037.4999961
ACGGGTA2000.037.1255
ACGGTCT1400.036.96428330
TTACGGG4750.036.9473723
AACCCGA1100.036.8181824
TCGATAG552.750312E-936.818181
TCGTAAG552.750312E-936.818181
CGATCGT552.750312E-936.8181810
CTACGAA2650.036.50943411
GACCGAT7500.036.39
AAGGGCG5900.036.2288135
ATTACGC250.00210734936.012
Produced by FastQC (version 0.11.5)