##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1584153
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719873017315876	31.0	31.0	34.0	30.0	34.0
2	31.853260385833945	33.0	31.0	34.0	30.0	34.0
3	31.83824415949722	33.0	31.0	34.0	30.0	34.0
4	35.51283682826091	37.0	35.0	37.0	33.0	37.0
5	35.35578002882297	37.0	35.0	37.0	33.0	37.0
6	35.306393384982385	37.0	35.0	37.0	32.0	37.0
7	35.67150963322356	37.0	35.0	37.0	35.0	37.0
8	35.58649574883234	37.0	35.0	37.0	35.0	37.0
9	37.31155765888774	39.0	37.0	39.0	34.0	39.0
10	36.65854371389632	39.0	35.0	39.0	32.0	39.0
11	36.598487014827484	39.0	35.0	39.0	32.0	39.0
12	36.5595917818544	39.0	35.0	39.0	32.0	39.0
13	36.50398288549149	39.0	35.0	39.0	32.0	39.0
14	37.57858615929143	39.0	36.0	41.0	32.0	41.0
15	37.70707059229759	39.0	36.0	41.0	33.0	41.0
16	37.704772834442124	39.0	36.0	41.0	33.0	41.0
17	37.644207977386024	39.0	36.0	41.0	32.0	41.0
18	37.59111651462958	39.0	36.0	41.0	32.0	41.0
19	37.512917628537146	39.0	36.0	41.0	32.0	41.0
20	37.41389752126215	39.0	35.0	41.0	32.0	41.0
21	37.30604619629543	39.0	35.0	41.0	32.0	41.0
22	37.30586818318685	39.0	35.0	41.0	32.0	41.0
23	36.82109808837909	39.0	35.0	41.0	31.0	41.0
24	36.91886642262458	39.0	35.0	41.0	31.0	41.0
25	36.90818689861396	39.0	35.0	41.0	31.0	41.0
26	36.92800569136946	39.0	35.0	40.0	31.0	41.0
27	34.51477666614273	39.0	35.0	40.0	23.0	41.0
28	35.38314165361553	38.0	35.0	40.0	23.0	41.0
29	36.038984239527366	38.0	35.0	40.0	28.0	41.0
30	36.29941426112251	38.0	35.0	40.0	30.0	41.0
31	36.33584129815744	38.0	35.0	40.0	30.0	41.0
32	36.06918776153566	38.0	35.0	40.0	29.0	41.0
33	36.033492977004116	38.0	35.0	40.0	29.0	41.0
34	35.933272859376586	38.0	35.0	40.0	29.0	41.0
35	35.83699554272851	38.0	35.0	40.0	28.0	41.0
36	35.72658259650425	38.0	35.0	40.0	28.0	41.0
37	35.66835463493741	38.0	35.0	40.0	27.0	41.0
38	35.58470678021631	38.0	35.0	40.0	27.0	41.0
39	35.5087412642592	38.0	34.0	40.0	27.0	41.0
40	35.42545006700742	38.0	34.0	40.0	27.0	41.0
41	35.29807285028656	38.0	34.0	40.0	26.0	41.0
42	35.19711101137327	38.0	34.0	40.0	26.0	41.0
43	35.09096217347693	38.0	34.0	40.0	26.0	41.0
44	34.96433235931126	38.0	34.0	40.0	25.0	41.0
45	34.91888346643285	38.0	34.0	40.0	25.0	41.0
46	34.74276727058561	37.0	33.0	40.0	24.0	41.0
47	34.64832689771758	37.0	33.0	40.0	24.0	41.0
48	34.53564523123713	37.0	33.0	40.0	23.0	41.0
49	34.4715137994878	37.0	33.0	40.0	24.0	41.0
50	34.36117029100093	37.0	33.0	40.0	24.0	41.0
51	32.799362182819465	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	5.0
11	13.0
12	25.0
13	33.0
14	47.0
15	119.0
16	206.0
17	413.0
18	895.0
19	1541.0
20	2645.0
21	4061.0
22	6118.0
23	8813.0
24	12318.0
25	16705.0
26	20708.0
27	24457.0
28	27740.0
29	32413.0
30	39346.0
31	48400.0
32	60947.0
33	79181.0
34	113423.0
35	133882.0
36	150701.0
37	201246.0
38	280402.0
39	317301.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14400566106936	18.11548505731454	23.193529917880408	17.546979363735698
2	32.64369035061639	24.36620705197036	25.326152208782865	17.663950388630393
3	31.885619633962126	24.06876103507679	27.93006736091779	16.1155519700433
4	27.811518205627866	26.338491294717116	26.90996387343899	18.940026626216028
5	23.729968001828105	30.33804184318055	26.799305370125232	19.132684784866107
6	24.159787596273844	35.59195355499122	25.95633123820742	14.29192761052752
7	80.48263014999183	6.137790983572925	9.18762266018497	4.19195620625028
8	81.94814516022126	4.731866177067493	9.04975718885739	4.270231473853851
9	75.91034451849032	7.53500451029667	11.15189000052394	5.4027609706890685
10	37.040551007383755	27.444129449617556	18.697562672292385	16.8177568707063
11	28.23950716881513	26.14217187355009	26.088389189680544	19.52993176795423
12	25.385174285564588	22.72009079931042	31.41842991175726	20.47630500336773
13	24.40269342670815	22.836746198126065	33.07571932761545	19.684841047550332
14	21.89586485648798	26.062318475551287	31.059121183370547	20.982695484590188
15	21.15414357072833	25.707049760976368	32.76274450763278	20.37606216066251
16	23.014317430197714	24.69654130630059	31.421586172547727	20.867555090953967
17	22.627927984228798	24.302134957923887	30.157377475534247	22.912559582313072
18	23.311069069717384	24.820771731013355	30.313991135957195	21.554168063312066
19	23.58964064708396	26.705185673353522	28.539667569988502	21.16550610957401
20	24.078734819174663	26.401111508799968	28.262989749096207	21.257163922929163
21	24.545924541379527	26.372831412117392	28.927698271568463	20.153545774934617
22	22.672999388316658	25.066013194432607	28.072540972999448	24.188446444251284
23	21.34162546168205	26.911352628186798	28.51965687657695	23.22736503355421
24	22.03827534335383	25.506500950350127	29.852167057096125	22.60305664919992
25	22.86635192434064	26.618641002478928	27.66102769113842	22.853979382042013
26	21.618808284300822	26.674696194117615	28.523570639957125	23.18292488162444
27	21.469580274127562	30.468142912963586	26.7158538348253	21.346422978083556
28	22.192363995144408	25.76733434207428	29.72313911598185	22.31716254679946
29	23.455183937410087	25.46420705575787	28.36531572392313	22.71529328290891
30	24.90624327321919	25.133935926643446	28.14702872765446	21.812792072482896
31	24.481915572548864	25.864420923989034	26.8430511446811	22.810612358781
32	24.252266037434516	26.019835205311608	27.63135883970803	22.096539917545844
33	23.973505084420506	26.02646335297159	26.688583741595668	23.311447821012237
34	23.1441028739017	25.980508195862395	28.153720000530253	22.721668929705654
35	23.648599598649877	25.330634099105325	27.586539936483405	23.434226365761386
36	23.595258791290995	27.98138816137078	26.26311978704077	22.16023326029746
37	22.521498870374263	26.90440885444777	27.94155615019509	22.632536124982877
38	21.58194315826817	28.25232158762443	27.729707925938975	22.43602732816843
39	22.151332604868344	26.27542920412359	26.667563044731157	24.905675146276906
40	22.853853131610393	26.14160374660781	28.16533504023917	22.83920808154263
41	21.63989210638114	25.757676184055455	27.79706253120753	24.80536917835588
42	22.38552715552096	26.44649853896688	27.629970084960227	23.53800422055193
43	22.41405975306678	25.11310460542637	28.58473897407637	23.88809666743048
44	22.650653061920156	25.12730777898347	28.21078519562189	24.011253963474488
45	23.095748958591752	24.417590977639154	28.033150838334432	24.453509225434665
46	23.00263926527299	25.97274379431785	27.374312960932436	23.650303979476732
47	22.25744609264383	25.407268111097853	28.486453013061237	23.84883278319708
48	21.700618563989714	25.28322706203252	28.97643093817327	24.039723435804497
49	22.60450852916353	24.70228570093924	28.599384024144133	24.093821745753093
50	21.50354164023298	23.796501979291143	29.793460606393445	24.906495774082426
51	21.569570615969543	23.99402077955854	28.3001705012079	26.136238103264013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2524.0
1	2753.0
2	2982.0
3	8948.5
4	14915.0
5	10448.5
6	5982.0
7	5903.0
8	5824.0
9	5932.5
10	6041.0
11	5994.5
12	5948.0
13	6044.5
14	6141.0
15	5954.0
16	5767.0
17	5707.0
18	5647.0
19	5672.5
20	5698.0
21	5832.5
22	5967.0
23	6078.0
24	6189.0
25	8342.0
26	11600.0
27	12705.0
28	16046.5
29	19388.0
30	22235.5
31	25083.0
32	28842.0
33	32601.0
34	36355.0
35	40109.0
36	43609.0
37	47109.0
38	50696.5
39	54284.0
40	58802.5
41	63321.0
42	71094.5
43	78868.0
44	90217.0
45	101566.0
46	115509.5
47	129453.0
48	136792.5
49	144132.0
50	142149.5
51	140167.0
52	126080.5
53	111994.0
54	101352.0
55	90710.0
56	83121.5
57	75533.0
58	71801.5
59	68070.0
60	64440.0
61	60810.0
62	55592.0
63	50374.0
64	44451.5
65	38529.0
66	33284.5
67	28040.0
68	24874.5
69	21709.0
70	20103.0
71	18497.0
72	16647.0
73	14797.0
74	12754.5
75	8578.5
76	6445.0
77	5191.5
78	3938.0
79	3340.0
80	2742.0
81	2019.5
82	1297.0
83	916.0
84	535.0
85	387.5
86	240.0
87	178.5
88	117.0
89	88.5
90	60.0
91	59.0
92	58.0
93	39.0
94	20.0
95	16.0
96	12.0
97	10.0
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1584153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.24933349409721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9799724545666	24.180541370655405
2	9.289159924576024	5.9913843257531
3	3.4483152364115948	3.336176041554425
4	1.8983286353650368	2.4487933297312607
5	1.2110497236149753	1.9527773207396788
6	0.9174638662910094	1.775255891568154
7	0.694434477565614	1.5676534339768857
8	0.6200253344507256	1.5996323028392485
9	0.4913751879530487	1.4261870076320307
>10	6.015901980739495	43.321479158408636
>50	0.383268049128377	7.630943633110611
>100	0.04482333831546061	2.432663721883717
>500	0.0036507668413525893	0.8145285731794296
>1k	0.0020282038007514384	0.9980640406444162
>5k	2.0282038007514382E-4	0.5239198483230731
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8010	0.5056329786327457	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1987	0.12542980381314178	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1940	0.12246291867010321	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	1610	0.1016315974530238	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8937564742799463E-4	0.0	0.0	0.11299413629870347	0.0
2	1.8937564742799463E-4	0.0	0.0	0.4610034510555483	0.0
3	1.8937564742799463E-4	0.0	0.0	0.7077599196542254	0.0
4	1.8937564742799463E-4	0.0	0.0	0.9850058674888095	0.0
5	1.8937564742799463E-4	0.0	0.0	1.7996367775082331	0.0
6	1.8937564742799463E-4	0.0	0.0	2.7882407822981743	0.0
7	1.8937564742799463E-4	0.0	0.0	3.5774322303464374	0.0
8	1.8937564742799463E-4	0.0	0.0	4.847764073293425	0.0
9	1.8937564742799463E-4	0.0	0.0	5.506665075911228	0.0
10	1.8937564742799463E-4	0.0	0.0	6.660783396553237	0.0
11	1.8937564742799463E-4	0.0	0.0	7.9920941979720395	0.0
12	1.8937564742799463E-4	0.0	0.0	8.989598858191096	0.0
13	1.8937564742799463E-4	0.0	0.0	9.484626800567874	0.0
14	1.8937564742799463E-4	0.0	0.0	9.710046946222997	0.0
15	1.8937564742799463E-4	0.0	0.0	9.988555398373768	0.0
16	2.525008632373262E-4	0.0	0.0	10.53149537954983	0.0
17	2.525008632373262E-4	0.0	0.0	11.142863094663205	0.0
18	2.525008632373262E-4	0.0	0.0	11.884963131717706	0.0
19	2.525008632373262E-4	0.0	0.0	12.243829983593756	0.0
20	2.525008632373262E-4	0.0	0.0	12.639435711070837	0.0
21	2.525008632373262E-4	0.0	0.0	13.07443157321294	0.0
22	2.525008632373262E-4	0.0	0.0	13.53726565552696	0.0
23	8.206278055213101E-4	0.0	0.0	14.02743295628642	0.0
24	8.206278055213101E-4	0.0	0.0	14.415337407434762	0.0
25	8.206278055213101E-4	0.0	0.0	14.767323610787594	0.0
26	8.206278055213101E-4	0.0	0.0	15.094249103464122	0.0
27	8.206278055213101E-4	0.0	0.0	15.430959004591097	0.0
28	8.206278055213101E-4	0.0	0.0	15.800494017938924	0.0
29	8.206278055213101E-4	0.0	0.0	16.202854143507604	0.0
30	9.468782371399732E-4	0.0	0.0	16.63456749442762	0.0
31	9.468782371399732E-4	0.0	0.0	17.030551973199557	0.0
32	0.0010100034529493048	0.0	0.0	17.431333968373004	0.0
33	0.0010731286687586364	0.0	0.0	17.84158474591785	0.0
34	0.0010731286687586364	0.0	0.0	18.23662234645265	0.0
35	0.0010731286687586364	0.0	0.0	18.644916242307403	0.0
36	0.0010731286687586364	0.0	0.0	19.039259465468298	0.0
37	0.0010731286687586364	0.0	0.0	19.43947333369946	0.0
38	0.001136253884567968	0.0	0.0	19.844042841821466	0.0
39	0.0011993791003772993	0.0	0.0	20.26111114267372	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGACG	45	3.8562575E-10	45.0	1
CGTTTTT	5840	0.0	41.60959	1
CGTAAGG	165	0.0	39.545452	2
GCTACGA	245	0.0	39.489796	10
CTATACG	40	3.4593177E-7	39.375004	1
CGTTCGA	80	0.0	39.375004	14
CATAACG	40	3.4593177E-7	39.375004	1
GCGTATG	110	0.0	38.863632	1
TGTTACG	120	0.0	37.499996	1
ACGGGTA	200	0.0	37.125	5
ACGGTCT	140	0.0	36.964283	30
TTACGGG	475	0.0	36.947372	3
AACCCGA	110	0.0	36.81818	24
TCGATAG	55	2.750312E-9	36.81818	1
TCGTAAG	55	2.750312E-9	36.81818	1
CGATCGT	55	2.750312E-9	36.81818	10
CTACGAA	265	0.0	36.509434	11
GACCGAT	750	0.0	36.3	9
AAGGGCG	590	0.0	36.228813	5
ATTACGC	25	0.002107349	36.0	12
>>END_MODULE