FastQCFastQC Report
Sat 18 Jun 2016
SRR3553990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671330
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23970.3570524183337554No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC21910.32636706239852237No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC20560.30625772719824823No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC19540.29106400726915227No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG19130.2849567276898098No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.18515484188104211No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.18470796776548046No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9190.13689243740038431No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC8910.13272161232180893No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC8510.126763290780987No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT8080.12035809512460341No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTTTCAT7710.1148466476993431No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7180.10695187165775401No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC7180.10695187165775401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG453.8380676E-1045.0000041
CTATAGT253.889065E-545.00000411
TAGCCGT253.889065E-545.00000444
CGTGTTC253.889065E-545.00000425
ATACGAG650.045.0000041
AGCCGTA207.0311874E-445.012
CAAACGT207.0311874E-445.045
CGCGATA207.0311874E-445.033
TGCGCGT207.0311874E-445.036
CGTAGTG207.0311874E-445.028
CGGTGTA351.2109194E-745.024
TTGCGAC207.0311874E-445.015
ACTCGTA207.0311874E-445.019
CATCGTT750.045.036
CATAAGT207.0311874E-445.014
CCGTTGT207.0311874E-445.019
ATCGCCA700.045.024
TACGTTA207.0311874E-445.025
TGCAACG207.0311874E-445.01
GCTACGA950.044.99999610