##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671330
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699268616030864	31.0	31.0	34.0	30.0	34.0
2	31.818910222990183	31.0	31.0	34.0	30.0	34.0
3	31.84325294564521	33.0	31.0	34.0	30.0	34.0
4	35.49188029732024	37.0	35.0	37.0	33.0	37.0
5	35.33352002740828	37.0	35.0	37.0	33.0	37.0
6	35.279570405016905	37.0	35.0	37.0	32.0	37.0
7	35.57243978371293	37.0	35.0	37.0	35.0	37.0
8	35.36866369743643	37.0	35.0	37.0	33.0	37.0
9	37.15679323134673	39.0	37.0	39.0	34.0	39.0
10	36.6224330806012	38.0	35.0	39.0	32.0	39.0
11	36.55991390225373	38.0	35.0	39.0	32.0	39.0
12	36.44578821146083	38.0	35.0	39.0	32.0	39.0
13	36.26726647103511	38.0	35.0	39.0	32.0	39.0
14	37.30301937944081	39.0	36.0	41.0	32.0	41.0
15	37.44351809095378	39.0	36.0	41.0	32.0	41.0
16	37.505209062607065	39.0	36.0	41.0	32.0	41.0
17	37.41173044553349	39.0	36.0	41.0	32.0	41.0
18	37.42954284777978	39.0	36.0	41.0	32.0	41.0
19	37.3180328601433	39.0	35.0	41.0	32.0	41.0
20	37.16552068282365	39.0	35.0	41.0	32.0	41.0
21	37.055209807397254	39.0	35.0	40.0	31.0	41.0
22	37.053876632952495	39.0	35.0	40.0	31.0	41.0
23	36.56157180522247	39.0	35.0	40.0	31.0	41.0
24	36.64363576780421	39.0	35.0	40.0	31.0	41.0
25	36.62524689794885	39.0	35.0	40.0	30.0	41.0
26	36.6353298675763	39.0	35.0	40.0	31.0	41.0
27	34.35549133809006	38.0	34.0	40.0	23.0	41.0
28	35.140005660405464	38.0	34.0	40.0	22.0	41.0
29	35.76686279475072	38.0	34.0	40.0	27.0	41.0
30	36.043215706135584	38.0	35.0	40.0	30.0	41.0
31	36.07641547376104	38.0	35.0	40.0	30.0	41.0
32	35.84511641070711	38.0	35.0	40.0	29.0	41.0
33	35.81024831305022	38.0	35.0	40.0	29.0	41.0
34	35.77677595221426	38.0	35.0	40.0	28.0	41.0
35	35.68377846960511	38.0	35.0	40.0	28.0	41.0
36	35.56447499739323	38.0	34.0	40.0	27.0	41.0
37	35.514182294847544	38.0	34.0	40.0	27.0	41.0
38	35.42192215452907	38.0	34.0	40.0	27.0	41.0
39	35.28899349053372	38.0	34.0	40.0	27.0	41.0
40	35.179180134955985	38.0	34.0	40.0	26.0	41.0
41	35.02247180969121	38.0	34.0	40.0	26.0	41.0
42	34.94518642098521	37.0	34.0	40.0	26.0	41.0
43	34.818378442792664	37.0	34.0	40.0	25.0	41.0
44	34.70248313050214	37.0	33.0	40.0	24.0	41.0
45	34.653277821637644	37.0	33.0	40.0	24.0	41.0
46	34.449468964592675	37.0	33.0	40.0	24.0	41.0
47	34.298030774730755	37.0	33.0	40.0	23.0	41.0
48	34.21891618131173	36.0	33.0	40.0	23.0	41.0
49	34.15808767670147	36.0	33.0	40.0	23.0	41.0
50	34.039390463706376	36.0	33.0	40.0	23.0	41.0
51	32.41812819328795	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	11.0
13	7.0
14	24.0
15	61.0
16	97.0
17	249.0
18	484.0
19	917.0
20	1532.0
21	2323.0
22	3283.0
23	4633.0
24	5695.0
25	7000.0
26	8209.0
27	9751.0
28	11561.0
29	14025.0
30	17446.0
31	21706.0
32	27570.0
33	36444.0
34	53279.0
35	63638.0
36	63798.0
37	83907.0
38	112055.0
39	121596.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58203863971519	18.883559501288488	23.763722759298705	14.770679099697615
2	29.417723027423175	27.12764214320826	25.732352196386277	17.72228263298229
3	31.413015953405925	25.89426958425812	26.268154260944694	16.424560201391266
4	27.69085248685445	26.83806771632431	26.335334336317462	19.135745460503774
5	24.044806577986982	31.222647580176666	24.99694636021033	19.735599481626025
6	23.33636214678325	37.329033411288044	24.54426288114638	14.790341560782327
7	78.0042601999017	7.659124424649575	9.86176694025293	4.474848435195805
8	78.92005422072602	7.80942308551681	7.954061340920263	5.316461352836906
9	73.22047279281426	9.051733126778187	10.10561124931107	7.62218283109648
10	40.80228799547167	25.750227161008745	16.67570345433691	16.771781389182667
11	32.08511462321064	26.99447365677089	21.86942338343289	19.050988336585583
12	26.80306257727198	22.97811806414133	28.44994265115517	21.768876707431517
13	24.806726945019587	25.342380051539482	29.934756379127997	19.91613662431293
14	21.027661507753265	29.4962239137235	27.870942755425798	21.605171823097432
15	20.260974483488	25.763186510360033	32.99301386799339	20.98282513815858
16	22.224092473150314	24.22966350379098	29.612858057885095	23.933385965173613
17	21.386054548433705	25.185974111092907	27.6600181728807	25.76795316759269
18	23.693265607078487	24.758017666423367	28.34626785634487	23.202448870153276
19	24.05568051479898	27.086976598692146	25.757377146857728	23.09996573965114
20	24.561393055576243	26.33235517554705	26.749735599481628	22.356516169395082
21	24.24232493706523	27.835937616373467	26.764780361372203	21.156957085189102
22	22.656070784859907	25.35578627500633	26.16984195552113	25.818300984612634
23	22.044598036733053	28.049096569496374	25.087810763707864	24.818494630062713
24	22.25358616477738	26.225999136043377	27.327990705018397	24.192423994160844
25	22.140080139424725	27.786185631507603	25.40106951871658	24.672664710351093
26	20.02219474773956	27.129429639670505	27.107532808007985	25.74084280458195
27	22.077517763246092	31.45248983361387	24.320378949249996	22.149613453890037
28	21.181833077622034	27.585241237543386	27.45892482087796	23.774000863956623
29	22.60661671607108	25.769144831900853	26.347995769591705	25.276242682436354
30	24.03676284390687	26.825257325011542	25.946702813817346	23.191277017264238
31	24.72122503090879	26.535236024012036	23.733335319440513	25.010203625638656
32	24.01129101931986	26.773122011529356	24.518791056559365	24.696795912591423
33	24.46784740738534	25.743226133198277	24.778722833777724	25.010203625638656
34	21.3570077309222	25.185080362861783	26.477887179181625	26.980024727034397
35	22.836161053431248	23.880654819537337	26.52257459073779	26.76060953629363
36	23.90627560216287	26.262791771557954	25.21904279564447	24.61188983063471
37	22.637897904160397	26.201570017726006	27.528488224867054	23.63204385324654
38	21.40363159697913	27.150879597217465	26.909865490891217	24.53562331491219
39	22.97230870063903	25.011097373869777	28.375463631894892	23.641130293596294
40	22.54122413716056	23.082239733067194	29.669164196445863	24.70737193332638
41	21.29802034766806	25.62122949964995	26.584690092800855	26.49606005988113
42	21.692759149747516	25.477633950516136	26.660658692446336	26.168948207290004
43	23.019230482773004	23.600315791041663	27.773375240194838	25.6070784859905
44	22.526179375269987	22.95934935128774	27.595966216316864	26.918505057125408
45	22.808156942189388	23.88989021792561	27.518805952363216	25.783146887521784
46	23.861290274529665	25.10002532286655	27.336332355175546	23.702352047428242
47	21.828310964801215	24.56571283869334	29.344882546586625	24.26109364991882
48	21.878956697898204	24.631850207796465	27.83355428775714	25.655638806548197
49	22.379902581442806	23.245050869170157	29.2678712406715	25.107175308715533
50	21.57150730639179	22.880848464987412	29.651587147900436	25.896057080720364
51	21.571805222468832	23.035764825048783	27.376551025576095	28.01587892690629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3583.0
1	3422.0
2	3261.0
3	3343.0
4	3425.0
5	2594.5
6	1764.0
7	1797.5
8	1831.0
9	1924.0
10	2017.0
11	2049.0
12	2081.0
13	2151.0
14	2221.0
15	2350.0
16	2479.0
17	2320.0
18	2161.0
19	2372.0
20	2583.0
21	2470.5
22	2358.0
23	2487.0
24	2616.0
25	3335.5
26	4452.0
27	4849.0
28	6566.0
29	8283.0
30	8904.5
31	9526.0
32	10360.5
33	11195.0
34	12662.0
35	14129.0
36	15422.5
37	16716.0
38	17283.0
39	17850.0
40	20957.5
41	24065.0
42	27686.5
43	31308.0
44	35536.5
45	39765.0
46	48954.0
47	58143.0
48	59695.0
49	61247.0
50	59393.5
51	57540.0
52	51641.5
53	45743.0
54	42551.0
55	39359.0
56	36708.0
57	34057.0
58	31401.5
59	28746.0
60	27582.0
61	26418.0
62	24239.5
63	22061.0
64	20673.5
65	19286.0
66	17266.5
67	15247.0
68	14086.0
69	12925.0
70	11761.0
71	10597.0
72	9773.5
73	8950.0
74	7966.5
75	5596.5
76	4210.0
77	3479.5
78	2749.0
79	2087.0
80	1425.0
81	1093.5
82	762.0
83	609.0
84	456.0
85	316.0
86	176.0
87	105.5
88	35.0
89	36.0
90	37.0
91	22.0
92	7.0
93	10.5
94	14.0
95	21.5
96	29.0
97	17.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	671330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.413121047899136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33266433328122	24.292603456579144
2	9.357198207026203	5.878775998929977
3	3.5317684410702497	3.3283160865746924
4	1.7801513304985184	2.2368043691411463
5	1.018277910619109	1.5993643633339942
6	0.6839398451207384	1.2890811086555483
7	0.5406852687621614	1.1889228257509157
8	0.3870112623071212	0.9725785303803074
9	0.3274482203308541	0.9257553523955039
>10	3.9772378842633795	30.472507228585606
>50	0.9456464874072045	19.542575933287896
>100	0.11092076094792103	5.426754412088366
>500	0.0037600257948447804	0.9274913270415474
>1k	0.003290022570489183	1.9184690072553114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2397	0.3570524183337554	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	2191	0.32636706239852237	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	2056	0.30625772719824823	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	1954	0.29106400726915227	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	1913	0.2849567276898098	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.18515484188104211	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1240	0.18470796776548046	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	919	0.13689243740038431	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	891	0.13272161232180893	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	851	0.126763290780987	No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	808	0.12035809512460341	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTTTCAT	771	0.1148466476993431	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	718	0.10695187165775401	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	718	0.10695187165775401	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4895803852054876E-4	0.0	0.0	0.1322747382062473	0.0
2	1.4895803852054876E-4	0.0	0.0	0.809735897397703	0.0
3	1.4895803852054876E-4	0.0	0.0	1.0972249117423623	0.0
4	1.4895803852054876E-4	0.0	0.0	1.5250323983733782	0.0
5	1.4895803852054876E-4	0.0	0.0	2.8589516333248923	0.0
6	1.4895803852054876E-4	0.0	0.0	3.7290155363234176	0.0
7	1.4895803852054876E-4	0.0	0.0	4.5435180909537785	0.0
8	1.4895803852054876E-4	0.0	0.0	5.87133004632595	0.0
9	1.4895803852054876E-4	0.0	0.0	6.4760996827193775	0.0
10	1.4895803852054876E-4	0.0	0.0	7.975511298467222	0.0
11	1.4895803852054876E-4	0.0	0.0	9.357841895937915	0.0
12	1.4895803852054876E-4	0.0	0.0	10.808097358973978	0.0
13	1.4895803852054876E-4	0.0	0.0	11.264504789000938	0.0
14	1.4895803852054876E-4	0.0	0.0	11.461278357886583	0.0
15	1.4895803852054876E-4	0.0	0.0	11.803286014329764	0.0
16	1.4895803852054876E-4	0.0	0.0	12.463319083014316	0.0
17	1.4895803852054876E-4	0.0	0.0	13.152845843325935	0.0
18	1.4895803852054876E-4	0.0	0.0	14.08249296173268	2.979160770410975E-4
19	2.979160770410975E-4	0.0	0.0	14.607123173402053	2.979160770410975E-4
20	2.979160770410975E-4	0.0	0.0	15.035824408264192	2.979160770410975E-4
21	2.979160770410975E-4	0.0	0.0	15.459907943932194	2.979160770410975E-4
22	2.979160770410975E-4	0.0	0.0	15.89546124856628	2.979160770410975E-4
23	2.979160770410975E-4	0.0	0.0	16.34159057393532	2.979160770410975E-4
24	2.979160770410975E-4	0.0	0.0	16.71011276123516	2.979160770410975E-4
25	4.468741155616463E-4	0.0	0.0	17.02515901270612	2.979160770410975E-4
26	4.468741155616463E-4	0.0	0.0	17.31830843251456	2.979160770410975E-4
27	5.95832154082195E-4	0.0	0.0	17.62724740440618	2.979160770410975E-4
28	5.95832154082195E-4	0.0	0.0	17.958083207960318	2.979160770410975E-4
29	5.95832154082195E-4	0.0	0.0	18.28623776682109	2.979160770410975E-4
30	5.95832154082195E-4	0.0	0.0	18.65624953450613	2.979160770410975E-4
31	7.447901926027438E-4	0.0	0.0	18.99334157567813	2.979160770410975E-4
32	8.937482311232926E-4	0.0	0.0	19.330880490965693	2.979160770410975E-4
33	8.937482311232926E-4	0.0	0.0	19.69344435672471	2.979160770410975E-4
34	8.937482311232926E-4	0.0	0.0	20.01400205562093	2.979160770410975E-4
35	8.937482311232926E-4	0.0	0.0	20.376268005302908	2.979160770410975E-4
36	8.937482311232926E-4	0.0	0.0	20.7060611025874	2.979160770410975E-4
37	8.937482311232926E-4	0.0	0.0	21.046132304529813	2.979160770410975E-4
38	8.937482311232926E-4	0.0	0.0	21.396779527207187	2.979160770410975E-4
39	8.937482311232926E-4	0.0	0.0	21.73789343541924	2.979160770410975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	45	3.8380676E-10	45.000004	1
CTATAGT	25	3.889065E-5	45.000004	11
TAGCCGT	25	3.889065E-5	45.000004	44
CGTGTTC	25	3.889065E-5	45.000004	25
ATACGAG	65	0.0	45.000004	1
AGCCGTA	20	7.0311874E-4	45.0	12
CAAACGT	20	7.0311874E-4	45.0	45
CGCGATA	20	7.0311874E-4	45.0	33
TGCGCGT	20	7.0311874E-4	45.0	36
CGTAGTG	20	7.0311874E-4	45.0	28
CGGTGTA	35	1.2109194E-7	45.0	24
TTGCGAC	20	7.0311874E-4	45.0	15
ACTCGTA	20	7.0311874E-4	45.0	19
CATCGTT	75	0.0	45.0	36
CATAAGT	20	7.0311874E-4	45.0	14
CCGTTGT	20	7.0311874E-4	45.0	19
ATCGCCA	70	0.0	45.0	24
TACGTTA	20	7.0311874E-4	45.0	25
TGCAACG	20	7.0311874E-4	45.0	1
GCTACGA	95	0.0	44.999996	10
>>END_MODULE