##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028843
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.832665430974405	31.0	31.0	34.0	30.0	34.0
2	31.953486586388788	33.0	31.0	34.0	30.0	34.0
3	31.970097478429654	33.0	31.0	34.0	30.0	34.0
4	35.60632769042507	37.0	35.0	37.0	33.0	37.0
5	35.44404248267228	37.0	35.0	37.0	33.0	37.0
6	35.38629120283658	37.0	35.0	37.0	33.0	37.0
7	35.70820232047066	37.0	35.0	37.0	35.0	37.0
8	35.600386064734856	37.0	35.0	37.0	35.0	37.0
9	37.365377419100874	39.0	37.0	39.0	34.0	39.0
10	36.74445080541929	39.0	37.0	39.0	32.0	39.0
11	36.62138052161506	39.0	35.0	39.0	32.0	39.0
12	36.480533959019986	38.0	35.0	39.0	32.0	39.0
13	36.37110326842871	39.0	35.0	39.0	32.0	39.0
14	37.32851659582657	39.0	36.0	41.0	32.0	41.0
15	37.50128639646671	39.0	36.0	41.0	32.0	41.0
16	37.55927094804552	39.0	36.0	41.0	32.0	41.0
17	37.51688838821861	39.0	36.0	41.0	32.0	41.0
18	37.48722691411615	39.0	36.0	41.0	32.0	41.0
19	37.42813918158553	39.0	36.0	41.0	32.0	41.0
20	37.31928486659286	39.0	35.0	41.0	32.0	41.0
21	37.21010299919424	39.0	35.0	41.0	32.0	41.0
22	37.222442102439345	39.0	35.0	41.0	32.0	41.0
23	36.74740266493527	39.0	35.0	41.0	31.0	41.0
24	36.83422154789409	39.0	35.0	41.0	31.0	41.0
25	36.82591707383926	39.0	35.0	41.0	31.0	41.0
26	36.86940864641155	39.0	35.0	41.0	31.0	41.0
27	34.43291639249137	38.0	35.0	40.0	23.0	41.0
28	35.30189057028137	38.0	34.0	40.0	22.0	41.0
29	35.98490440232378	38.0	34.0	40.0	28.0	41.0
30	36.27197541315827	38.0	35.0	40.0	30.0	41.0
31	36.362165072805084	38.0	35.0	40.0	30.0	41.0
32	36.14232978209503	38.0	35.0	40.0	30.0	41.0
33	36.17255888410574	38.0	35.0	40.0	30.0	41.0
34	36.09495909482788	38.0	35.0	40.0	29.0	41.0
35	36.001642621857755	38.0	35.0	40.0	29.0	41.0
36	35.88350797935156	38.0	35.0	40.0	29.0	41.0
37	35.77501717949191	38.0	35.0	40.0	28.0	41.0
38	35.652991758703706	38.0	34.0	40.0	28.0	41.0
39	35.54612705728668	38.0	34.0	40.0	27.0	41.0
40	35.4254108741567	38.0	34.0	40.0	27.0	41.0
41	35.277311504282	38.0	34.0	40.0	26.0	41.0
42	35.19517263566939	38.0	34.0	40.0	26.0	41.0
43	35.093491426777454	38.0	34.0	40.0	26.0	41.0
44	34.963700000874766	37.0	34.0	40.0	26.0	41.0
45	34.899891431442896	37.0	34.0	40.0	26.0	41.0
46	34.78526850063615	37.0	33.0	40.0	25.0	41.0
47	34.676138147414136	37.0	33.0	40.0	24.0	41.0
48	34.583502050361425	37.0	33.0	40.0	24.0	41.0
49	34.519332881693316	37.0	33.0	40.0	24.0	41.0
50	34.40898173968234	36.0	33.0	40.0	24.0	41.0
51	32.82858997922909	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	10.0
12	15.0
13	18.0
14	55.0
15	104.0
16	206.0
17	370.0
18	611.0
19	1069.0
20	1815.0
21	2788.0
22	4160.0
23	5635.0
24	7464.0
25	9481.0
26	12253.0
27	14838.0
28	17621.0
29	20916.0
30	25359.0
31	31811.0
32	39959.0
33	53257.0
34	79942.0
35	91858.0
36	95571.0
37	127372.0
38	176964.0
39	207278.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.148968307117805	17.81875368739448	23.72665217141974	13.305625834067976
2	31.65137926777944	24.401876671173348	26.880486138312648	17.066257922734568
3	33.90390953721802	24.236642519801368	25.472399578944504	16.387048364036104
4	28.42639741923695	27.213675944726262	25.17604726863088	19.18387936740591
5	24.546505151903645	30.97936225449364	25.046678647762583	19.427453945840135
6	25.32485520142529	36.407692913301645	23.953800531276396	14.313651353996674
7	82.0292308933433	6.617724959007351	7.048500111290061	4.304544036359289
8	83.83349062976568	5.26086098656452	6.812020881708871	4.093627501960941
9	78.18996678793557	7.397435760363826	9.107220440825277	5.305377010875323
10	41.50954032831054	26.20341490392606	16.53964696265611	15.74739780510729
11	32.719569458119466	27.192875880965317	22.103664018708393	17.98389064220683
12	27.78966275709705	24.521428439518957	27.57203966008419	20.116869143299805
13	26.025933986040627	24.563903336077516	29.187835267382876	20.222327410498977
14	22.152553888202572	27.780623477051407	29.484673560494652	20.58214907425137
15	21.89964844004382	25.81044921333964	31.46583103544467	20.824071311171867
16	24.868031371161585	24.406833695714507	29.522580218750576	21.20255471437333
17	25.091875047990804	23.8889704260028	27.406319525914064	23.612835000092335
18	26.68249674634517	23.900828406277732	27.92185007819463	21.49482476918247
19	25.83115208054096	26.776583016067562	25.757671481460243	21.634593421931235
20	26.529509361486642	26.96358919679679	25.479203338118644	21.027698103597924
21	25.582037298207794	26.790287730975475	27.720944789438235	19.9067301813785
22	24.363386833559638	25.180421113814255	26.75850445597628	23.69768759664983
23	22.985139617998083	27.560667662607415	26.41258190025106	23.041610819143447
24	22.709198585206877	26.79796625918629	27.77547205939099	22.717363096215845
25	23.985778199394854	26.779596109416108	25.768168709900348	23.46645698128869
26	23.22502072716634	26.317912451170876	26.39635007479275	24.06071674687003
27	21.542839869639973	30.696714659087927	25.820849245220117	21.939596226051982
28	22.007536621233754	26.810213025699742	27.245264826606196	23.936985526460305
29	22.78724742259023	26.906923602532164	27.00975756262131	23.296071412256293
30	24.567305215664586	25.715488174580575	27.456472950683438	22.260733659071402
31	25.91269999407101	25.836303498201378	25.36878804637831	22.8822084613493
32	24.711253320477468	25.74221722847898	26.195833572274875	23.350695878768676
33	25.509723057842642	25.43799199683528	25.083807733541462	23.968477211780613
34	22.77169597304934	25.518373551649766	29.080724658669983	22.629205816630915
35	22.97658632075059	26.772695153682342	27.081683016747938	23.16903550881913
36	25.394642331240043	26.87008610643218	25.180032327575734	22.555239234752047
37	24.04244379365948	27.395627904354697	25.723263899351018	22.838664402634805
38	23.46830371592167	27.743105605034003	25.681858164948395	23.10673251409593
39	24.53571633378465	24.948024139737548	26.409082824104356	24.107176702373444
40	24.803006872768734	24.11835430673096	28.20770516006815	22.870933660432154
41	22.937318910659837	24.818266732630732	27.111619557114157	25.132794799595278
42	23.81062999894056	24.949482088132008	27.273063042660546	23.966824870266894
43	24.32674373057891	24.05089989434734	27.408943833024086	24.21341254204966
44	23.66920900467807	25.07253293262432	27.01763048395139	24.240627578746224
45	23.735788648025014	24.925280144783997	27.240793784863193	24.0981374223278
46	23.546838536103177	24.78036007437481	27.961214684845014	23.711586704677
47	21.915977462061754	26.15909327273452	28.866892227482715	23.058037037721014
48	22.91029826708254	25.94691318306097	27.227769445872696	23.915019103983795
49	24.206997569114044	25.183045420924284	27.28676775756845	23.323189252393224
50	21.968366407702632	25.69468811081963	27.941192193561115	24.395753287916623
51	22.398169594389035	24.99322053996577	26.436589450479808	26.172020415165388
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3354.0
1	3352.5
2	3351.0
3	3786.0
4	4221.0
5	3206.0
6	2191.0
7	2198.5
8	2206.0
9	2309.0
10	2412.0
11	2480.5
12	2549.0
13	2536.5
14	2524.0
15	2668.5
16	2813.0
17	2615.0
18	2417.0
19	2506.0
20	2595.0
21	3085.5
22	3576.0
23	4143.0
24	4710.0
25	5613.5
26	7155.0
27	7793.0
28	9785.0
29	11777.0
30	13829.5
31	15882.0
32	18562.5
33	21243.0
34	23492.5
35	25742.0
36	26552.0
37	27362.0
38	30932.5
39	34503.0
40	37574.5
41	40646.0
42	44557.5
43	48469.0
44	54958.0
45	61447.0
46	68710.0
47	75973.0
48	80410.5
49	84848.0
50	85537.5
51	86227.0
52	79046.0
53	71865.0
54	67126.5
55	62388.0
56	58854.5
57	55321.0
58	51976.5
59	48632.0
60	46612.0
61	44592.0
62	40705.5
63	36819.0
64	34215.5
65	31612.0
66	27311.5
67	23011.0
68	20597.5
69	18184.0
70	16432.5
71	14681.0
72	13323.5
73	11966.0
74	10521.0
75	6984.5
76	4893.0
77	4614.5
78	4336.0
79	3202.0
80	2068.0
81	1583.5
82	1099.0
83	763.0
84	427.0
85	348.0
86	269.0
87	186.5
88	104.0
89	67.5
90	31.0
91	44.5
92	58.0
93	50.0
94	42.0
95	31.0
96	20.0
97	10.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1028843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.391707149618995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92548745043727	21.556542048848872
2	9.235242688629468	5.2440861174245565
3	3.6294541967279157	3.091392019993639
4	1.8975002656484774	2.154930874344633
5	1.156749944396266	1.6421052833318424
6	0.7983589334417409	1.3600063823136044
7	0.5954911623452103	1.1834907484044004
8	0.4611391416389596	1.0474021971712013
9	0.3765786609371629	0.9622539954111238
>10	4.548228171754107	31.232337702519843
>50	1.2155021777114121	23.401725333027894
>100	0.1558248317428646	5.859259638175253
>500	0.002392047855701488	0.44483881045552726
>1k	0.002050326733458418	0.8196288485775995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1913	0.1859370185732906	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1723	0.16746967224348128	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1596	0.1551257091703982	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	1103	0.10720780527252458	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1064	0.10341713944693214	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1049	0.1019591910524735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8878623852230126E-4	0.0	0.0	0.11624708531816808	0.0
2	3.8878623852230126E-4	0.0	0.0	0.505324913519361	0.0
3	3.8878623852230126E-4	0.0	0.0	0.6766824481480653	0.0
4	3.8878623852230126E-4	0.0	0.0	0.9101485843807072	0.0
5	3.8878623852230126E-4	0.0	0.0	1.5980086368862887	0.0
6	3.8878623852230126E-4	0.0	0.0	2.2040291861829258	0.0
7	3.8878623852230126E-4	0.0	0.0	2.715769072637905	0.0
8	3.8878623852230126E-4	0.0	0.0	3.625237281101198	0.0
9	3.8878623852230126E-4	0.0	0.0	4.073507814117412	0.0
10	3.8878623852230126E-4	0.0	0.0	4.988710619598908	0.0
11	3.8878623852230126E-4	0.0	0.0	6.006650188609924	0.0
12	3.8878623852230126E-4	0.0	0.0	6.745246845242666	0.0
13	3.8878623852230126E-4	0.0	0.0	7.057928177574227	0.0
14	3.8878623852230126E-4	0.0	0.0	7.175633211286853	0.0
15	3.8878623852230126E-4	0.0	0.0	7.38120393490552	0.0
16	3.8878623852230126E-4	0.0	0.0	7.852412855994549	0.0
17	3.8878623852230126E-4	0.0	0.0	8.342963892450063	0.0
18	5.831793577834519E-4	0.0	0.0	8.963175139452764	0.0
19	5.831793577834519E-4	0.0	0.0	9.262832132793827	0.0
20	5.831793577834519E-4	0.0	0.0	9.564724647006395	0.0
21	5.831793577834519E-4	0.0	0.0	9.907731305942695	0.0
22	5.831793577834519E-4	0.0	0.0	10.261235193319097	0.0
23	5.831793577834519E-4	0.0	0.0	10.622903591704468	0.0
24	5.831793577834519E-4	0.0	0.0	10.906134366467965	0.0
25	5.831793577834519E-4	0.0	0.0	11.165357591002708	0.0
26	5.831793577834519E-4	0.0	0.0	11.415055552693657	0.0
27	5.831793577834519E-4	0.0	0.0	11.717531246264008	0.0
28	5.831793577834519E-4	0.0	0.0	12.006205028366816	0.0
29	5.831793577834519E-4	0.0	0.0	12.322288240285447	0.0
30	5.831793577834519E-4	0.0	0.0	12.684053835230449	0.0
31	5.831793577834519E-4	0.0	0.0	12.969131344626925	0.0
32	5.831793577834519E-4	0.0	0.0	13.256249981775644	0.0
33	5.831793577834519E-4	0.0	0.0	13.542396653328058	0.0
34	5.831793577834519E-4	0.0	0.0	13.852745268228485	0.0
35	5.831793577834519E-4	0.0	0.0	14.186907040238404	0.0
36	5.831793577834519E-4	0.0	0.0	14.474220070506384	0.0
37	5.831793577834519E-4	0.0	0.0	14.793607965452455	0.0
38	5.831793577834519E-4	0.0	0.0	15.13214358264575	0.0
39	5.831793577834519E-4	0.0	0.0	15.633580633779887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.03294E-4	45.000004	32
TAAACGT	20	7.03294E-4	45.000004	39
TCGATAG	45	3.8562575E-10	45.000004	1
TAATTCG	20	7.03294E-4	45.000004	16
AACGCAT	20	7.03294E-4	45.000004	42
CGTACGA	20	7.03294E-4	45.000004	39
ATACGCG	20	7.03294E-4	45.000004	1
TACGTAC	20	7.03294E-4	45.000004	37
GTACGAA	25	3.89052E-5	45.0	40
GTATGCG	30	2.1652795E-6	44.999996	1
GTGTACG	55	6.184564E-11	40.909092	1
TATAGCG	55	6.184564E-11	40.909092	1
GTACGAT	55	6.184564E-11	40.909092	10
GGTACGA	100	0.0	40.5	9
ACGTGAG	440	0.0	39.886364	44
AACACGT	430	0.0	39.76744	41
CGTGTAC	85	0.0	39.705883	18
CGTAAGG	165	0.0	39.545456	2
CTAACGC	80	0.0	39.375004	25
GACGCAA	40	3.4577533E-7	39.375004	26
>>END_MODULE