FastQCFastQC Report
Sat 18 Jun 2016
SRR3553988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645975
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87860.533786965172618No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC25600.15553091632618962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50900.040.6679761
TATCGCG451.9292202E-840.01
TCGTGTA4300.037.6744217
AATTGCG900.037.51
ATTTCGA301.1401365E-437.49999611
AACACGT5650.037.43362841
ACACGAC5950.036.3025226
TAAGGGA10250.036.2195134
CTACGAA2250.036.011
TTACGTA250.002107372336.014
CGTAAGG2250.036.02
TTACGAG250.002107372336.01
AATGCGG2400.035.6252
CGTGTAC4550.035.60439318
ATAGGGC7800.035.480774
CACGACC6150.035.12195227
CGAATAT2250.035.014
CGCATCG1100.034.77272821
TTAACGG1750.034.7142872
TAGTAGG5850.034.230772
Produced by FastQC (version 0.11.5)