##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1645975
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843766764379776	31.0	31.0	34.0	30.0	34.0
2	31.97293944318717	33.0	31.0	34.0	30.0	34.0
3	31.958636674311578	33.0	31.0	34.0	30.0	34.0
4	35.61999301325962	37.0	35.0	37.0	33.0	37.0
5	35.45326569358587	37.0	35.0	37.0	33.0	37.0
6	35.394787284132505	37.0	35.0	37.0	33.0	37.0
7	35.70229803004298	37.0	35.0	37.0	35.0	37.0
8	35.59082549856468	37.0	35.0	37.0	35.0	37.0
9	37.32156867510138	39.0	37.0	39.0	34.0	39.0
10	36.76451586445724	39.0	37.0	39.0	32.0	39.0
11	36.67517368125275	39.0	35.0	39.0	32.0	39.0
12	36.56669147465788	39.0	35.0	39.0	32.0	39.0
13	36.488442412551834	39.0	35.0	39.0	32.0	39.0
14	37.534768146539285	39.0	36.0	41.0	32.0	41.0
15	37.64437491456432	40.0	36.0	41.0	32.0	41.0
16	37.67456492352557	40.0	36.0	41.0	33.0	41.0
17	37.62481386412309	40.0	36.0	41.0	32.0	41.0
18	37.580096903051384	39.0	36.0	41.0	32.0	41.0
19	37.50627804189006	39.0	36.0	41.0	32.0	41.0
20	37.400683485472136	39.0	35.0	41.0	32.0	41.0
21	37.28364950864989	39.0	35.0	41.0	32.0	41.0
22	37.27853703731831	39.0	35.0	41.0	32.0	41.0
23	36.80140585367335	39.0	35.0	41.0	31.0	41.0
24	36.88400978143653	39.0	35.0	41.0	31.0	41.0
25	36.86475067968833	39.0	35.0	41.0	31.0	41.0
26	36.882574158173725	39.0	35.0	41.0	31.0	41.0
27	34.60420662525251	38.0	35.0	40.0	24.0	41.0
28	35.37232521757621	38.0	35.0	40.0	23.0	41.0
29	35.99767432676681	38.0	34.0	40.0	28.0	41.0
30	36.23331520831118	38.0	35.0	40.0	30.0	41.0
31	36.25995838332903	38.0	35.0	40.0	30.0	41.0
32	35.99478424641929	38.0	35.0	40.0	29.0	41.0
33	35.95104117620255	38.0	35.0	40.0	29.0	41.0
34	35.825436595331034	38.0	35.0	40.0	28.0	41.0
35	35.72025759808017	38.0	35.0	40.0	28.0	41.0
36	35.611818527012865	38.0	34.0	40.0	27.0	41.0
37	35.51254970458239	38.0	34.0	40.0	27.0	41.0
38	35.397276386336365	38.0	34.0	40.0	27.0	41.0
39	35.297621166785646	38.0	34.0	40.0	26.0	41.0
40	35.179386685703	38.0	34.0	40.0	26.0	41.0
41	35.04662160725406	38.0	34.0	40.0	26.0	41.0
42	34.92990416014824	38.0	34.0	40.0	25.0	41.0
43	34.82950895365968	37.0	34.0	40.0	25.0	41.0
44	34.68451221920139	37.0	33.0	40.0	24.0	41.0
45	34.612983793800026	37.0	33.0	40.0	24.0	41.0
46	34.48068409301478	37.0	33.0	40.0	23.0	41.0
47	34.36550737404882	37.0	33.0	40.0	23.0	41.0
48	34.24784702076277	36.0	33.0	40.0	23.0	41.0
49	34.17775361108158	36.0	33.0	40.0	24.0	41.0
50	34.062244566290495	36.0	33.0	40.0	23.0	41.0
51	32.4590981029481	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	7.0
10	9.0
11	11.0
12	11.0
13	19.0
14	61.0
15	141.0
16	278.0
17	563.0
18	1053.0
19	1829.0
20	3001.0
21	4428.0
22	6516.0
23	9207.0
24	12578.0
25	17052.0
26	22124.0
27	26509.0
28	30471.0
29	35117.0
30	42177.0
31	50992.0
32	64319.0
33	83459.0
34	123361.0
35	140282.0
36	158129.0
37	209056.0
38	283734.0
39	319423.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42774039702912	17.090296025152266	22.432843755221068	18.049119822597547
2	31.855222588435428	25.008946065401965	25.84717264843026	17.288658697732348
3	32.799647625267696	23.759899147921445	27.269672990173	16.170780236637857
4	28.488403529822747	26.02147663239114	26.448579109646257	19.041540728139854
5	24.6791111651149	30.087455763301385	25.373107124956334	19.86032594662738
6	24.80535852610155	35.221130333085256	25.124136150306047	14.849374990507147
7	81.31168456386033	5.738847795379638	8.700435911845563	4.249031728914473
8	83.01262169838546	4.6570573672139615	8.292531782074454	4.037789152326129
9	76.74691292395084	6.939595072829174	10.494934613223165	5.81855738999681
10	41.16283661659503	24.739743920776437	17.74595604428986	16.351463418338675
11	31.09075168213369	25.436291559713847	23.417002080833548	20.055954677318915
12	26.65842433815823	21.922872461610897	29.946202099059825	21.472501101171037
13	24.90189705189933	22.33885690851927	31.42866689955801	21.33057914002339
14	22.45987940278558	24.62206291104057	30.479077750269596	22.438979935904253
15	22.2675314023603	23.6077097161257	31.996111727091847	22.12864715442215
16	25.344856392108024	23.331885356703474	29.57596561308647	21.747292638102035
17	25.01016115068576	23.17100806512857	28.286456355655464	23.532374428530204
18	26.145779856923706	22.77318914321299	28.53913334042133	22.54189765944197
19	25.82025850939413	24.625343641306824	26.786737344127342	22.76766050517171
20	26.34705873418491	24.460274305502818	26.7273196737496	22.465347286562675
21	26.400157961086894	25.304697823478485	26.823736691019	21.47140752441562
22	25.123893133249293	23.03133401175595	27.43893437020611	24.40583848478865
23	24.084752198544933	25.444250368322724	26.71425750694877	23.756739926183567
24	23.388265313871717	24.5049894439466	28.262458421300447	23.84428682088124
25	25.06465772566412	24.68737374504473	26.20386093348927	24.04410759580188
26	23.781041631859537	24.446422333267517	26.599128176308874	25.173407858564072
27	22.917723537720804	28.42114855936451	25.808046902291952	22.853081000622733
28	23.397742979085344	25.16235058248151	27.59288567566336	23.847020762769784
29	25.07942101186227	24.820182566563894	26.42767964276493	23.672716778808915
30	25.87846109448807	23.996172481356034	26.89737085921718	23.227995564938713
31	26.487097313142666	24.226370388371635	25.61320797703489	23.67332432145081
32	25.914184601831742	24.895639362687767	26.24335120521272	22.946824830267776
33	25.836905177782164	24.810158112972555	25.348137122374276	24.004799586871002
34	24.543264630386243	24.978690441835386	27.611415726241283	22.866629201537084
35	25.206275915490817	24.926927808745578	26.635276963501873	23.231519312261728
36	26.045049286896827	26.03514634183387	25.977672807910206	21.942131563359105
37	25.214538495420648	26.18059205030453	26.18217166117347	22.422697793101353
38	24.33366241893103	26.461945047768044	27.07538085329364	22.12901168000729
39	25.172496544601223	24.351159647017724	26.36163975759049	24.114704050790564
40	25.357311016266955	23.788696669147466	28.172238338978417	22.681753975607162
41	23.28206686006774	24.253527544464525	27.830920882759457	24.633484712708274
42	24.58457753003539	24.632694907273805	27.51937301599356	23.263354546697247
43	24.637433739880617	23.450963714515712	27.77739637600814	24.13420616959553
44	24.30857090782059	23.954495056121754	27.455945564179284	24.28098847187837
45	24.582997919166452	23.47648050547548	27.344157718069837	24.596363857288235
46	24.256747520466593	24.114704050790564	27.112926988563007	24.51562144017983
47	23.682012181229968	24.380564710885647	28.107960327465488	23.8294627804189
48	23.39336867206367	24.676680994547304	28.313856528805115	23.616093804583908
49	24.092103464511915	24.678746639529763	27.487477027293856	23.74167286866447
50	22.945548990719786	24.23748841871839	28.5447227327268	24.27223985783502
51	23.30017163079634	23.740032503531342	27.27100958398518	25.688786281687147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2345.0
1	2585.0
2	2825.0
3	9167.5
4	15510.0
5	10606.0
6	5702.0
7	5437.5
8	5173.0
9	5171.5
10	5170.0
11	5078.5
12	4987.0
13	5036.5
14	5086.0
15	5032.0
16	4978.0
17	4663.5
18	4349.0
19	4338.0
20	4327.0
21	4781.0
22	5235.0
23	5735.5
24	6236.0
25	7485.0
26	10371.0
27	12008.0
28	14045.0
29	16082.0
30	19724.0
31	23366.0
32	25932.5
33	28499.0
34	32067.0
35	35635.0
36	38432.0
37	41229.0
38	44346.0
39	47463.0
40	51665.0
41	55867.0
42	61088.0
43	66309.0
44	74304.0
45	82299.0
46	95613.0
47	108927.0
48	117698.0
49	126469.0
50	128965.0
51	131461.0
52	124262.0
53	117063.0
54	109402.0
55	101741.0
56	97998.0
57	94255.0
58	91663.5
59	89072.0
60	86852.0
61	84632.0
62	79234.0
63	73836.0
64	66700.0
65	59564.0
66	53561.5
67	47559.0
68	41709.0
69	35859.0
70	31625.5
71	27392.0
72	24081.5
73	20771.0
74	17730.0
75	12399.0
76	10109.0
77	8055.5
78	6002.0
79	4731.5
80	3461.0
81	2688.0
82	1915.0
83	1370.5
84	826.0
85	636.5
86	447.0
87	332.5
88	218.0
89	181.5
90	145.0
91	105.0
92	65.0
93	49.0
94	33.0
95	32.5
96	32.0
97	24.0
98	16.0
99	9.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1645975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.660283971903905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31018234896196	21.58411212097115
2	8.190570923739294	4.694881771327748
3	3.159898068461993	2.716907278931741
4	1.7627738121052055	2.0208639213268307
5	1.145877409539728	1.6420585977199114
6	0.8715759134146607	1.498776790892142
7	0.7071172598943426	1.4186327029004504
8	0.5741408947605512	1.3164032866976314
9	0.4879366477000299	1.258596259504352
>10	7.2342325142773705	48.71993325793593
>50	0.5054245955759112	8.865813113552132
>100	0.043903592328119724	2.213834187661254
>500	0.004829393906835301	0.9338392540947479
>1k	0.0013171074291369001	0.562581055724263
>5k	2.1951790485615002E-4	0.5527664007597154
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8786	0.533786965172618	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2560	0.15553091632618962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.430170567596713E-4	0.0	0.0	0.05650146569662358	0.0
2	2.430170567596713E-4	0.0	0.0	0.27211834930664197	0.0
3	2.430170567596713E-4	0.0	0.0	0.4484272239857835	0.0
4	2.430170567596713E-4	0.0	0.0	0.6369477057670986	0.0
5	2.430170567596713E-4	0.0	0.0	1.1564574188550858	0.0
6	2.430170567596713E-4	0.0	0.0	1.9077446498276098	0.0
7	2.430170567596713E-4	0.0	0.0	2.4787132246844576	0.0
8	2.430170567596713E-4	0.0	0.0	3.3838302525858532	0.0
9	2.430170567596713E-4	0.0	0.0	3.891978918270326	0.0
10	2.430170567596713E-4	0.0	0.0	4.73233190054527	0.0
11	3.0377132094958916E-4	0.0	0.0	5.5607770470389895	0.0
12	3.0377132094958916E-4	0.0	0.0	6.21783441425295	0.0
13	3.0377132094958916E-4	0.0	0.0	6.514375977763939	0.0
14	3.0377132094958916E-4	0.0	0.0	6.65435380245751	0.0
15	3.0377132094958916E-4	0.0	0.0	6.834672458573186	0.0
16	3.0377132094958916E-4	0.0	0.0	7.1720408876198	0.0
17	3.0377132094958916E-4	0.0	0.0	7.540150974346512	0.0
18	3.0377132094958916E-4	0.0	0.0	8.004131289964915	0.0
19	3.0377132094958916E-4	0.0	0.0	8.243260073816431	0.0
20	3.64525585139507E-4	0.0	0.0	8.485851850726773	0.0
21	4.252798493294248E-4	0.0	0.0	8.759367548109783	0.0
22	4.252798493294248E-4	0.0	0.0	9.050866507693009	0.0
23	4.252798493294248E-4	0.0	0.0	9.334285150138975	0.0
24	4.252798493294248E-4	0.0	0.0	9.58191953097708	0.0
25	4.252798493294248E-4	0.0	0.0	9.799966585154696	0.0
26	4.252798493294248E-4	0.0	0.0	10.014186120688345	0.0
27	4.252798493294248E-4	0.0	0.0	10.246571181214781	0.0
28	4.860341135193426E-4	0.0	0.0	10.49183614574948	0.0
29	4.860341135193426E-4	0.0	0.0	10.75071006546272	0.0
30	4.860341135193426E-4	0.0	0.0	11.081577788241011	0.0
31	5.467883777092604E-4	0.0	0.0	11.340694725011012	0.0
32	5.467883777092604E-4	0.0	0.0	11.626968817873905	0.0
33	6.682969060890961E-4	0.0	0.0	11.895624174121721	0.0
34	6.682969060890961E-4	0.0	0.0	12.158872400856636	0.0
35	6.682969060890961E-4	0.0	0.0	12.441683500660703	0.0
36	7.29051170279014E-4	0.0	0.0	12.702258539771261	0.0
37	7.29051170279014E-4	0.0	0.0	12.982639469007731	0.0
38	7.29051170279014E-4	0.0	0.0	13.292060936526983	0.0
39	7.29051170279014E-4	0.0	0.0	13.661689879858443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5090	0.0	40.667976	1
TATCGCG	45	1.9292202E-8	40.0	1
TCGTGTA	430	0.0	37.67442	17
AATTGCG	90	0.0	37.5	1
ATTTCGA	30	1.1401365E-4	37.499996	11
AACACGT	565	0.0	37.433628	41
ACACGAC	595	0.0	36.30252	26
TAAGGGA	1025	0.0	36.219513	4
CTACGAA	225	0.0	36.0	11
TTACGTA	25	0.0021073723	36.0	14
CGTAAGG	225	0.0	36.0	2
TTACGAG	25	0.0021073723	36.0	1
AATGCGG	240	0.0	35.625	2
CGTGTAC	455	0.0	35.604393	18
ATAGGGC	780	0.0	35.48077	4
CACGACC	615	0.0	35.121952	27
CGAATAT	225	0.0	35.0	14
CGCATCG	110	0.0	34.772728	21
TTAACGG	175	0.0	34.714287	2
TAGTAGG	585	0.0	34.23077	2
>>END_MODULE