FastQCFastQC Report
Sat 18 Jun 2016
SRR3553987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824152
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15600.18928547161203274No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC12500.15167105097118005No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.14475485104689426No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8540.10362166202351021No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.0321234E-445.01
ACACGTT207.0321234E-445.014
GATAACG351.2113742E-745.01
CGAGTAA207.0321234E-445.024
ACGCATT700.045.017
TCGATTG253.8898415E-545.01
TCGATAT207.0321234E-445.014
AACATCG207.0321234E-445.013
CTACGCA700.045.015
TTACGCG253.8898415E-545.01
TAGTCCG253.8898415E-545.01
CTAACGG700.041.7857132
ACGTAGG650.041.538462
TCGATAG603.6379788E-1241.2500041
GTTATAG1150.041.086961
TTTCGAC451.9272193E-840.024
TGCGACG451.9272193E-840.01
AAGGGCG3750.039.65
CACTACC403.4566256E-739.37518
ATACGAG403.4566256E-739.3751
Produced by FastQC (version 0.11.5)