##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	824152
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8478108407187	31.0	31.0	34.0	30.0	34.0
2	31.968203680874403	33.0	31.0	34.0	30.0	34.0
3	31.98694051582718	33.0	31.0	34.0	30.0	34.0
4	35.62252351507974	37.0	35.0	37.0	33.0	37.0
5	35.466492831419444	37.0	35.0	37.0	33.0	37.0
6	35.4042361117852	37.0	35.0	37.0	33.0	37.0
7	35.698861375086146	37.0	35.0	37.0	35.0	37.0
8	35.548225327366794	37.0	35.0	37.0	35.0	37.0
9	37.3166855143226	39.0	37.0	39.0	34.0	39.0
10	36.74812898591522	39.0	37.0	39.0	32.0	39.0
11	36.69285398809928	39.0	35.0	39.0	32.0	39.0
12	36.61421194148652	39.0	35.0	39.0	32.0	39.0
13	36.48776317960765	39.0	35.0	39.0	32.0	39.0
14	37.57655141284617	39.0	36.0	41.0	32.0	41.0
15	37.70880492918782	40.0	36.0	41.0	33.0	41.0
16	37.76020442830934	40.0	36.0	41.0	33.0	41.0
17	37.71127898737126	40.0	36.0	41.0	33.0	41.0
18	37.67881288888457	39.0	36.0	41.0	33.0	41.0
19	37.6094142828022	39.0	36.0	41.0	33.0	41.0
20	37.532053553228046	39.0	36.0	41.0	32.0	41.0
21	37.4246000737728	39.0	36.0	41.0	32.0	41.0
22	37.42731437890098	39.0	35.0	41.0	32.0	41.0
23	36.929890360030676	39.0	35.0	41.0	31.0	41.0
24	36.994436705850376	39.0	35.0	41.0	31.0	41.0
25	37.00326274764849	39.0	35.0	41.0	31.0	41.0
26	37.025540191615136	39.0	35.0	41.0	31.0	41.0
27	34.57339665498597	39.0	35.0	41.0	23.0	41.0
28	35.449957046758364	39.0	35.0	40.0	23.0	41.0
29	36.148937331948474	39.0	35.0	40.0	29.0	41.0
30	36.41735869111523	38.0	35.0	40.0	30.0	41.0
31	36.511188470088044	39.0	35.0	40.0	30.0	41.0
32	36.29001446335142	38.0	35.0	40.0	30.0	41.0
33	36.31049854881138	39.0	35.0	40.0	30.0	41.0
34	36.25854454032751	38.0	35.0	40.0	30.0	41.0
35	36.18571452838797	38.0	35.0	40.0	30.0	41.0
36	36.06311457109854	38.0	35.0	40.0	29.0	41.0
37	36.02776308253817	38.0	35.0	40.0	29.0	41.0
38	35.926382511963816	38.0	35.0	40.0	29.0	41.0
39	35.80227919121715	38.0	35.0	40.0	28.0	41.0
40	35.66738659858861	38.0	34.0	40.0	28.0	41.0
41	35.560118764499755	38.0	34.0	40.0	27.0	41.0
42	35.473402964501695	38.0	34.0	40.0	27.0	41.0
43	35.366662945670214	38.0	34.0	40.0	27.0	41.0
44	35.24532003805123	38.0	34.0	40.0	27.0	41.0
45	35.17342310641726	38.0	34.0	40.0	26.0	41.0
46	35.00355880953999	37.0	34.0	40.0	26.0	41.0
47	34.88845018880012	37.0	34.0	40.0	26.0	41.0
48	34.78845771168425	37.0	33.0	40.0	26.0	41.0
49	34.71615430163368	37.0	33.0	40.0	26.0	41.0
50	34.602592725613725	37.0	33.0	40.0	25.0	41.0
51	32.99171754724856	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	4.0
11	5.0
12	7.0
13	14.0
14	20.0
15	59.0
16	113.0
17	285.0
18	474.0
19	810.0
20	1334.0
21	1986.0
22	2977.0
23	4027.0
24	5238.0
25	6868.0
26	8586.0
27	10486.0
28	12868.0
29	15940.0
30	19317.0
31	24327.0
32	30876.0
33	41246.0
34	61146.0
35	73601.0
36	79512.0
37	105388.0
38	146477.0
39	170116.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8726108776051	18.4263339772275	23.508891563692135	14.192163581475262
2	30.427639561634262	25.381725701084267	26.594730098331375	17.595904638950095
3	33.098870111338684	24.587212067676838	25.639930498257602	16.673987322726877
4	28.366126636833982	27.09839932439647	25.170842271813935	19.364631766955608
5	24.851119696366688	31.01551655519856	24.435541016705656	19.697822731729097
6	24.87029091720945	37.10832467797203	23.414006154204564	14.607378250613964
7	81.7193915685456	6.7102913054873365	7.336268067055592	4.234049058911463
8	82.96139547073841	5.841155515002087	6.841335093526436	4.356113920733069
9	76.56500257234103	8.179923120977683	9.300226171871207	5.954848134810083
10	39.78101126976577	26.680393907919896	16.649113270367604	16.889481551946727
11	31.449902445180015	27.091847134994516	21.834079150447977	19.624171269377495
12	28.064361913821724	23.485594890262963	28.16956095477533	20.280482241139982
13	25.512890825964142	24.086940273153495	29.697191780157056	20.7029771207253
14	21.97846999097254	27.504999077840008	27.75822906454149	22.758301866645958
15	20.24213979945447	26.296969491064754	31.686630621535834	21.774260087944942
16	23.116002873256388	24.30316252341801	30.355201467690424	22.225633135635174
17	23.0075277376018	24.139357788369136	27.590905561110084	25.262208912918975
18	23.94279210631049	25.235636144788824	27.96802046224483	22.85355128665586
19	25.125583630204133	26.798818664518198	25.954071579029108	22.121526126248554
20	26.75538007552005	25.681427697803315	25.98112969452237	21.582062532154264
21	25.688829245090712	26.643628845164486	25.991321989147632	21.67621992059717
22	24.306802628641318	25.404779700831888	24.816781370426813	25.47163630009998
23	23.16514429377105	27.766965317077428	25.23466545006261	23.833224939088907
24	22.379609586581118	25.795848338655976	27.979304788437087	23.845237286325823
25	23.139906230889448	27.20371970219086	25.94497131597084	23.711402750948853
26	22.378517555014124	27.30564264844349	25.65315621390229	24.662683582640096
27	21.73579630941865	31.5504906861841	24.465632553218338	22.248080451178907
28	21.75618089866918	28.159975344353956	27.26814956464342	22.815694192333453
29	23.166843009541928	26.688644813092733	25.902624758539687	24.24188741882565
30	23.49044836389404	26.841893242994008	26.419398363408693	23.248260029703257
31	24.427896795736707	27.43826381541269	24.184980440501267	23.948858948349333
32	24.179762956347858	28.160339354876285	24.216892029625605	23.443005659150256
33	23.65279705685359	27.637134897446103	23.774255234471312	24.935812811228995
34	23.161989535910852	27.618691697648007	25.43001776371349	23.789301002727655
35	23.010197148098893	26.552990225104107	25.00837224201361	25.42844038478339
36	23.701695803686697	28.60358283423446	24.574229025713702	23.120492336365135
37	23.437788174996847	29.398581815005	24.550447004921423	22.613183005076735
38	23.2164697774197	27.835399295275632	24.290543491977207	24.657587435327464
39	24.16144109339054	26.332278511730845	25.11781807239441	24.3884623224842
40	24.469758005804753	25.379662974791056	27.218280123084092	22.9322988963201
41	21.99363709606966	27.10143274541589	26.434444131665032	24.470486026849418
42	22.884249507372427	27.530237140721614	26.036216620235102	23.549296731670857
43	22.983260369446413	26.339437385336684	26.35945796406488	24.31784428115202
44	23.139906230889448	26.5930313825605	26.220891291897612	24.046171094652443
45	23.712858793038176	25.6115376775158	25.397863500907597	25.277740028538425
46	23.802769392053893	26.60201030877799	25.768790223162718	23.826430076005398
47	23.375421038837494	26.958983294343764	26.64338617148293	23.02220949533581
48	22.713771246080817	26.169323134567414	26.57264679330997	24.544258826041798
49	22.71765402498568	26.29769751210942	26.537216435803103	24.447432027101797
50	22.05345615857269	25.423829584833868	27.555717877284774	24.96699637930867
51	21.933332686203517	25.517016278550557	26.230598239159768	26.319052796086158
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3216.0
1	3064.0
2	2912.0
3	3091.5
4	3271.0
5	2555.0
6	1839.0
7	1806.5
8	1774.0
9	1896.0
10	2018.0
11	2014.0
12	2010.0
13	2006.0
14	2002.0
15	2054.5
16	2107.0
17	2014.5
18	1922.0
19	2074.0
20	2226.0
21	2399.5
22	2573.0
23	3041.0
24	3509.0
25	4488.5
26	6317.5
27	7167.0
28	8356.5
29	9546.0
30	11239.0
31	12932.0
32	14985.0
33	17038.0
34	18774.0
35	20510.0
36	21556.0
37	22602.0
38	25360.0
39	28118.0
40	30293.0
41	32468.0
42	35247.0
43	38026.0
44	44397.5
45	50769.0
46	56050.0
47	61331.0
48	63615.5
49	65900.0
50	65968.0
51	66036.0
52	60466.5
53	54897.0
54	50890.5
55	46884.0
56	45642.5
57	44401.0
58	41130.5
59	37860.0
60	37552.5
61	37245.0
62	35298.5
63	33352.0
64	28858.5
65	24365.0
66	22067.0
67	19769.0
68	17930.5
69	16092.0
70	14172.5
71	12253.0
72	10912.0
73	9571.0
74	8336.5
75	5835.0
76	4568.0
77	3956.5
78	3345.0
79	2422.0
80	1499.0
81	1149.0
82	799.0
83	577.5
84	356.0
85	350.0
86	344.0
87	231.0
88	118.0
89	65.0
90	12.0
91	10.5
92	9.0
93	14.0
94	19.0
95	10.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	824152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.040327121531735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98636850989132	22.066689983045016
2	9.309656894261325	5.407109631971442
3	3.5285203309979662	3.0740815400146912
4	1.8005960079108503	2.0915958833382096
5	1.0880937954822776	1.5799299879857194
6	0.753603286701845	1.3130931579409826
7	0.5673566954740042	1.1533356821809444
8	0.44003711451333866	1.0223057400865818
9	0.37783886302851044	0.9875307763418009
>10	4.947053151619478	35.48012358559111
>50	1.1148599391393996	21.119028214323947
>100	0.08142235505356034	3.5557708337749987
>500	0.003340404309889655	0.6640090398905979
>1k	0.0012526516162086206	0.4853959435138903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1560	0.18928547161203274	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1250	0.15167105097118005	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.14475485104689426	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	854	0.10362166202351021	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10168027257107912	0.0
2	0.0	0.0	0.0	0.5051252681544182	0.0
3	0.0	0.0	0.0	0.6935613818810122	0.0
4	0.0	0.0	0.0	0.9410885370659782	0.0
5	0.0	0.0	0.0	1.678695192149021	0.0
6	0.0	0.0	0.0	2.313772216775546	0.0
7	0.0	0.0	0.0	2.8449849056970073	0.0
8	0.0	0.0	0.0	3.7985711373630107	0.0
9	0.0	0.0	0.0	4.256617711295974	0.0
10	0.0	0.0	0.0	5.203894427241576	0.0
11	0.0	0.0	0.0	6.216329026684398	0.0
12	0.0	0.0	0.0	7.0280724914821535	0.0
13	0.0	0.0	0.0	7.331899940787622	0.0
14	0.0	0.0	0.0	7.455784855220881	0.0
15	0.0	0.0	0.0	7.682806084314544	0.0
16	0.0	0.0	0.0	8.161601258020365	0.0
17	1.2133684077694406E-4	0.0	0.0	8.688081810151525	0.0
18	1.2133684077694406E-4	0.0	0.0	9.320853434803288	0.0
19	1.2133684077694406E-4	0.0	0.0	9.616672652617478	0.0
20	1.2133684077694406E-4	0.0	0.0	9.913826575680215	0.0
21	1.2133684077694406E-4	0.0	0.0	10.260728603461498	0.0
22	1.2133684077694406E-4	0.0	0.0	10.639663557207893	0.0
23	1.2133684077694406E-4	0.0	0.0	11.021631931973713	0.0
24	2.4267368155388811E-4	0.0	0.0	11.318543181354896	0.0
25	2.4267368155388811E-4	0.0	0.0	11.583785515293295	0.0
26	2.4267368155388811E-4	0.0	0.0	11.813718828565605	0.0
27	3.640105223308322E-4	0.0	0.0	12.086120036109843	0.0
28	3.640105223308322E-4	0.0	0.0	12.37490171715897	0.0
29	3.640105223308322E-4	0.0	0.0	12.674482377037245	0.0
30	3.640105223308322E-4	0.0	0.0	13.011313447034043	0.0
31	3.640105223308322E-4	0.0	0.0	13.311379454275425	0.0
32	3.640105223308322E-4	0.0	0.0	13.621152408778963	0.0
33	3.640105223308322E-4	0.0	0.0	13.918427668682476	0.0
34	4.8534736310777623E-4	0.0	0.0	14.208786728661703	0.0
35	4.8534736310777623E-4	0.0	0.0	14.524141177840981	0.0
36	4.8534736310777623E-4	0.0	0.0	14.812922858890108	0.0
37	4.8534736310777623E-4	0.0	0.0	15.114930255583921	0.0
38	4.8534736310777623E-4	0.0	0.0	15.405167978722371	0.0
39	4.8534736310777623E-4	0.0	0.0	15.714819596385134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.0321234E-4	45.0	1
ACACGTT	20	7.0321234E-4	45.0	14
GATAACG	35	1.2113742E-7	45.0	1
CGAGTAA	20	7.0321234E-4	45.0	24
ACGCATT	70	0.0	45.0	17
TCGATTG	25	3.8898415E-5	45.0	1
TCGATAT	20	7.0321234E-4	45.0	14
AACATCG	20	7.0321234E-4	45.0	13
CTACGCA	70	0.0	45.0	15
TTACGCG	25	3.8898415E-5	45.0	1
TAGTCCG	25	3.8898415E-5	45.0	1
CTAACGG	70	0.0	41.785713	2
ACGTAGG	65	0.0	41.53846	2
TCGATAG	60	3.6379788E-12	41.250004	1
GTTATAG	115	0.0	41.08696	1
TTTCGAC	45	1.9272193E-8	40.0	24
TGCGACG	45	1.9272193E-8	40.0	1
AAGGGCG	375	0.0	39.6	5
CACTACC	40	3.4566256E-7	39.375	18
ATACGAG	40	3.4566256E-7	39.375	1
>>END_MODULE