FastQCFastQC Report
Sat 18 Jun 2016
SRR3553986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775243
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC20860.26907692168778047TruSeq Adapter, Index 19 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18910.2439235181743015No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC14310.18458728424506896TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT12490.1611107742991552TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10750.13866619885635859No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT9700.1251220585029468No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8900.11480271347177595No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.11028800002063868No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC8120.10474135206638435TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.0318626E-445.00000429
ATTACGG650.045.0000042
GCACGAT207.0318626E-445.00000440
TATGCGT207.0318626E-445.00000411
CGTTATC207.0318626E-445.00000417
ACGATAT207.0318626E-445.00000434
TCTACGT207.0318626E-445.00000425
ATGCGTT302.1645828E-645.00000412
CGTCGCA207.0318626E-445.00000426
CGCACAT207.0318626E-445.00000436
TGCGTAT302.1645828E-645.00000415
GGCTAAT207.0318626E-445.0000048
CGATTCG253.8896273E-545.013
TAGCACG453.8380676E-1045.01
CATACGA1350.045.018
TACCGTT253.8896273E-545.017
GTACGAG950.045.01
CGAATAT1550.042.0967714
AGGCGAT700.041.7857176
CGTAAGG1650.040.909092
Produced by FastQC (version 0.11.5)