##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775243
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717514379362342	31.0	31.0	34.0	30.0	34.0
2	31.830417043430252	31.0	31.0	34.0	30.0	34.0
3	31.861110645307342	33.0	31.0	34.0	30.0	34.0
4	35.502808796725674	37.0	35.0	37.0	33.0	37.0
5	35.347567149913	37.0	35.0	37.0	33.0	37.0
6	35.29613037460513	37.0	35.0	37.0	32.0	37.0
7	35.63105503693681	37.0	35.0	37.0	35.0	37.0
8	35.49867589904069	37.0	35.0	37.0	33.0	37.0
9	37.24469875896977	39.0	37.0	39.0	34.0	39.0
10	36.63358972606009	38.0	35.0	39.0	32.0	39.0
11	36.5917989585201	39.0	35.0	39.0	32.0	39.0
12	36.50541443134604	38.0	35.0	39.0	32.0	39.0
13	36.38254070014176	38.0	35.0	39.0	32.0	39.0
14	37.42652432850087	39.0	36.0	41.0	32.0	41.0
15	37.56552719598887	39.0	36.0	41.0	32.0	41.0
16	37.61013385480423	39.0	36.0	41.0	33.0	41.0
17	37.569950325252854	39.0	36.0	41.0	32.0	41.0
18	37.56052360356688	39.0	36.0	41.0	33.0	41.0
19	37.48275701941198	39.0	36.0	41.0	32.0	41.0
20	37.41358257991365	39.0	36.0	41.0	32.0	41.0
21	37.294457350791944	39.0	35.0	41.0	32.0	41.0
22	37.28302867616993	39.0	35.0	41.0	32.0	41.0
23	36.78553692197156	39.0	35.0	41.0	31.0	41.0
24	36.885702934434754	39.0	35.0	41.0	31.0	41.0
25	36.900322866507665	39.0	35.0	41.0	31.0	41.0
26	36.91420625532898	39.0	35.0	40.0	31.0	41.0
27	34.46015378403933	38.0	35.0	40.0	23.0	41.0
28	35.34347939936252	38.0	34.0	40.0	23.0	41.0
29	35.9898715628519	38.0	34.0	40.0	27.0	41.0
30	36.27383413974715	38.0	35.0	40.0	30.0	41.0
31	36.36617937859484	38.0	35.0	40.0	30.0	41.0
32	36.12254222224515	38.0	35.0	40.0	30.0	41.0
33	36.15551768929226	38.0	35.0	40.0	30.0	41.0
34	36.131878649662106	38.0	35.0	40.0	30.0	41.0
35	36.01842906030754	38.0	35.0	40.0	29.0	41.0
36	35.9222914621609	38.0	35.0	40.0	29.0	41.0
37	35.84204952511664	38.0	35.0	40.0	29.0	41.0
38	35.730422590078206	38.0	34.0	40.0	29.0	41.0
39	35.64898618884659	38.0	34.0	40.0	28.0	41.0
40	35.56239656469004	38.0	34.0	40.0	28.0	41.0
41	35.47068983531615	38.0	34.0	40.0	27.0	41.0
42	35.40705817401769	38.0	34.0	40.0	27.0	41.0
43	35.26297689885623	38.0	34.0	40.0	27.0	41.0
44	35.124110762689895	38.0	34.0	40.0	26.0	41.0
45	35.00025411387139	37.0	34.0	40.0	26.0	41.0
46	34.85536921971563	37.0	34.0	40.0	26.0	41.0
47	34.73357643990336	37.0	33.0	40.0	26.0	41.0
48	34.65428259268384	37.0	33.0	40.0	26.0	41.0
49	34.60617509606665	37.0	33.0	40.0	26.0	41.0
50	34.49385676491113	36.0	33.0	40.0	25.0	41.0
51	32.8591177217982	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	3.0
12	3.0
13	8.0
14	20.0
15	40.0
16	82.0
17	205.0
18	442.0
19	780.0
20	1300.0
21	1949.0
22	2721.0
23	3925.0
24	5014.0
25	6381.0
26	8025.0
27	10148.0
28	12715.0
29	15467.0
30	19624.0
31	24354.0
32	31213.0
33	41588.0
34	59928.0
35	70649.0
36	75832.0
37	101324.0
38	137816.0
39	143656.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26449900224833	19.613979100746477	23.356160584487704	13.765361312517493
2	29.254827196117862	25.93393297327419	26.235515831810158	18.5757239987978
3	32.430992604899366	24.7389528186646	26.42964851020906	16.400406066226978
4	27.998575930385698	27.38109212208301	25.3102575579528	19.310074389578492
5	24.450011157791813	31.525986045665682	24.114116477027203	19.909886319515298
6	24.48045322563377	36.46134179863604	24.239496519155928	14.818708456574262
7	81.38751333452866	6.53279036379561	7.587685409607052	4.492010892068681
8	82.51025807392006	5.585345498121234	6.846756436368984	5.057639991589734
9	76.00455599083126	7.939704066982868	9.644459866132296	6.411280076053573
10	39.542182257692104	26.866414788653366	16.591700924742305	16.999702028912225
11	29.969312847713553	27.198955682282843	22.87566608147381	19.95606538852979
12	26.620814376911493	23.108238320113824	29.29442768267498	20.9765196202997
13	24.209570418565534	25.013060421055076	30.449549367101667	20.327819793277722
14	21.216186408648642	28.499322148023264	28.134533301171377	22.149958142156716
15	20.134590057569046	26.356123176861963	32.059883159215886	21.449403606353105
16	21.543696621575428	25.026733553221376	29.779178915514233	23.650390909688962
17	21.088226530262126	25.057562596502	27.749492739695814	26.104718133540068
18	23.70443847928972	24.163133365925265	28.593486171432698	23.538941983352316
19	23.638652654716005	26.802047874021433	26.610108056441657	22.949191414820902
20	25.043115513458357	26.239256594383953	26.34967358621748	22.367954305940202
21	24.61434672741321	27.59728240048604	26.548320978067526	21.240049894033223
22	23.1508056183674	25.117543789495684	25.416030844522297	26.315619747614623
23	22.173305660289742	28.144594662576765	25.547731485482615	24.134368191650875
24	22.161051438065225	25.74689484458421	27.77851589759598	24.31353781975458
25	21.813418502327657	27.627724468327997	26.174889679752027	24.38396734959232
26	22.146991330460256	27.086345829630194	26.607270236558083	24.159392603351463
27	21.52989449759624	31.912832492521698	24.49361039054851	22.063662619333552
28	21.910807321059334	27.056290737226906	27.538720117434146	23.494181824279615
29	23.341197534192503	25.834093310097607	26.635003476329356	24.18970567938053
30	24.751207040889113	26.63745432077426	25.69916787381505	22.912170764521576
31	25.26949614507967	26.855966451809305	23.554163017273293	24.32037438583773
32	25.173010269038226	26.988183060021182	24.7103166362031	23.128490034737496
33	24.148557291068737	25.988754493752282	24.367843372981117	25.494844842197868
34	22.884953492001863	25.93367498964841	26.813270161742835	24.368101356606896
35	23.365061019577087	25.57095001180275	25.922710685552786	25.141278283067376
36	24.68967794614076	28.117248398244165	24.17435565364666	23.018718001968416
37	23.378605159930498	27.972906559620657	25.633640032867117	23.014848247581725
38	22.44702628724155	28.58935843342023	26.06034494990603	22.90327032943219
39	22.9392590452284	24.445625436153566	27.207985109185117	25.407130409432916
40	23.6972149377679	24.807834446747666	27.463646882332377	24.031303733152058
41	20.987225940769537	25.910972430579832	25.68433381533274	27.417467813317884
42	22.675857763307764	26.245577193215546	25.709874194284886	25.3686908491918
43	23.197371662820558	24.066776481696706	27.358389563014434	25.377462292468294
44	23.647037122553833	23.834204243056693	27.57251597241123	24.946242661978243
45	23.061414292034886	23.89857115768862	27.382640023837684	25.657374526438808
46	24.004989403322572	25.25311418484269	26.929233801530618	23.812662610304123
47	21.79432771401999	25.69142836504167	28.00167173389505	24.51257218704329
48	22.30887605563675	25.342118535736535	27.678160267167844	24.670845141458873
49	23.31849497512393	23.913921183422488	27.950977951429422	24.81660589002416
50	22.37221103576556	23.149386708425617	28.669978316476254	25.80842393933257
51	22.22283851643936	23.72610910385518	26.142899710155397	27.908152669550063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3619.0
1	3334.0
2	3049.0
3	2920.0
4	2791.0
5	2182.5
6	1574.0
7	1601.0
8	1628.0
9	1643.0
10	1658.0
11	1686.5
12	1715.0
13	1728.0
14	1741.0
15	1729.5
16	1718.0
17	1977.5
18	2237.0
19	2052.5
20	1868.0
21	1858.5
22	1849.0
23	2401.5
24	2954.0
25	3356.0
26	4370.5
27	4983.0
28	6529.0
29	8075.0
30	9428.0
31	10781.0
32	12294.5
33	13808.0
34	16438.5
35	19069.0
36	18902.5
37	18736.0
38	20321.5
39	21907.0
40	25273.0
41	28639.0
42	32810.5
43	36982.0
44	42442.5
45	47903.0
46	54981.5
47	62060.0
48	67187.5
49	72315.0
50	72502.0
51	72689.0
52	65699.5
53	58710.0
54	52534.0
55	46358.0
56	43144.5
57	39931.0
58	38941.0
59	37951.0
60	34904.5
61	31858.0
62	29490.5
63	27123.0
64	24456.5
65	21790.0
66	18731.5
67	15673.0
68	14074.0
69	12475.0
70	11604.0
71	10733.0
72	9682.0
73	8631.0
74	7069.0
75	4694.5
76	3882.0
77	3076.5
78	2271.0
79	1559.5
80	848.0
81	815.5
82	783.0
83	556.0
84	329.0
85	242.5
86	156.0
87	100.0
88	44.0
89	29.5
90	15.0
91	15.5
92	16.0
93	32.5
94	49.0
95	25.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.169044696479958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72481990720678	23.602702963206237
2	9.793544356086786	6.10510843543656
3	3.7662467185528716	3.521709369256363
4	1.9384500054206877	2.4167853944339694
5	1.1651698718278736	1.8158615906997406
6	0.7710392381723992	1.441953388640122
7	0.5137426995350225	1.1209008415008173
8	0.4297359879114484	1.0715568171918326
9	0.3684643871929628	1.03362146581307
>10	4.554170857345719	34.27942909989244
>50	0.8944312811002726	18.010920885244477
>100	0.07439816565202859	3.7346202582514323
>500	0.003719908282601429	0.8487246286991768
>1k	0.0020666157125563494	0.9961048617337636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	2086	0.26907692168778047	TruSeq Adapter, Index 19 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1891	0.2439235181743015	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1431	0.18458728424506896	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	1249	0.1611107742991552	TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1075	0.13866619885635859	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	970	0.1251220585029468	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	890	0.11480271347177595	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.11028800002063868	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	812	0.10474135206638435	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.289918128896359E-4	0.0	0.0	0.17826668541347682	0.0
2	1.289918128896359E-4	0.0	0.0	0.861923293728547	0.0
3	1.289918128896359E-4	0.0	0.0	1.1491880610337661	0.0
4	1.289918128896359E-4	0.0	0.0	1.541452164031149	0.0
5	1.289918128896359E-4	0.0	0.0	2.6937360285742664	0.0
6	1.289918128896359E-4	0.0	0.0	3.628152721146789	0.0
7	2.579836257792718E-4	0.0	0.0	4.46363269323296	0.0
8	2.579836257792718E-4	0.0	0.0	5.8573892315054765	0.0
9	2.579836257792718E-4	0.0	0.0	6.52556682227379	0.0
10	2.579836257792718E-4	0.0	0.0	7.8977817277937366	0.0
11	2.579836257792718E-4	0.0	0.0	9.407759889479815	0.0
12	2.579836257792718E-4	0.0	0.0	10.6562716464386	0.0
13	2.579836257792718E-4	0.0	0.0	11.145150617290321	0.0
14	2.579836257792718E-4	0.0	0.0	11.32625512258737	0.0
15	2.579836257792718E-4	0.0	0.0	11.654152310952824	0.0
16	2.579836257792718E-4	0.0	0.0	12.357157691201339	0.0
17	2.579836257792718E-4	0.0	0.0	13.1255619205849	0.0
18	2.579836257792718E-4	0.0	0.0	14.13917958627166	0.0
19	2.579836257792718E-4	0.0	0.0	14.595036653023632	0.0
20	2.579836257792718E-4	0.0	0.0	15.01761383205008	0.0
21	2.579836257792718E-4	0.0	0.0	15.51745710699742	0.0
22	2.579836257792718E-4	0.0	0.0	16.004917167907355	0.0
23	0.001160926316006723	0.0	0.0	16.53042981361973	0.0
24	0.001160926316006723	0.0	0.0	16.945654459311466	0.0
25	0.001289918128896359	0.0	0.0	17.300639928383745	0.0
26	0.001289918128896359	0.0	0.0	17.629956026690987	0.0
27	0.001289918128896359	0.0	0.0	17.99422890629132	0.0
28	0.001289918128896359	0.0	0.0	18.382236279463342	0.0
29	0.001289918128896359	0.0	0.0	18.771275587138483	0.0
30	0.001289918128896359	0.0	0.0	19.221457014123313	0.0
31	0.001289918128896359	0.0	0.0	19.635391741686156	0.0
32	0.001289918128896359	0.0	0.0	20.04068401778539	0.0
33	0.001289918128896359	0.0	0.0	20.436946866982353	0.0
34	0.001289918128896359	0.0	0.0	20.822245412083696	0.0
35	0.0014189099417859948	0.0	0.0	21.23875997590433	0.0
36	0.0014189099417859948	0.0	0.0	21.629476177147037	0.0
37	0.0014189099417859948	0.0	0.0	22.01090496786169	0.0
38	0.0014189099417859948	0.0	0.0	22.397235447466148	0.0
39	0.0014189099417859948	0.0	0.0	22.764604130575833	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.0318626E-4	45.000004	29
ATTACGG	65	0.0	45.000004	2
GCACGAT	20	7.0318626E-4	45.000004	40
TATGCGT	20	7.0318626E-4	45.000004	11
CGTTATC	20	7.0318626E-4	45.000004	17
ACGATAT	20	7.0318626E-4	45.000004	34
TCTACGT	20	7.0318626E-4	45.000004	25
ATGCGTT	30	2.1645828E-6	45.000004	12
CGTCGCA	20	7.0318626E-4	45.000004	26
CGCACAT	20	7.0318626E-4	45.000004	36
TGCGTAT	30	2.1645828E-6	45.000004	15
GGCTAAT	20	7.0318626E-4	45.000004	8
CGATTCG	25	3.8896273E-5	45.0	13
TAGCACG	45	3.8380676E-10	45.0	1
CATACGA	135	0.0	45.0	18
TACCGTT	25	3.8896273E-5	45.0	17
GTACGAG	95	0.0	45.0	1
CGAATAT	155	0.0	42.09677	14
AGGCGAT	70	0.0	41.785717	6
CGTAAGG	165	0.0	40.90909	2
>>END_MODULE