FastQCFastQC Report
Sat 18 Jun 2016
SRR3553985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742990
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13530.18210204713387798No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC11310.1522227755420665No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.1483196274512443No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8920.12005545162115237No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT8350.11238374675298457No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT8220.1106340596777884No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT8150.109691920483452No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG207.031674E-445.01
CGCAATA207.031674E-445.02
ATACGCG207.031674E-445.01
CGATCTA207.031674E-445.010
TGTCGGT253.8894686E-545.037
CCGCATT207.031674E-445.023
GCACTAG207.031674E-445.01
CACTATC302.164461E-644.99999629
TATAGCG302.164461E-644.9999961
ATTGGGT800.042.18754
CGACGGT556.002665E-1140.90909228
CGTAAGG2100.040.7142872
GCGATCT501.0804797E-940.59
ACGTAAG501.0804797E-940.51
ATACGCA501.0804797E-940.527
AATACGC501.0804797E-940.526
GTTAGCG451.9266736E-840.01
TCGTAAC1300.039.80769334
CGTAACC1300.039.80769335
TCGATAG403.4560253E-739.3751