##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742990
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.808908599039018	31.0	31.0	34.0	30.0	34.0
2	31.932054267217595	33.0	31.0	34.0	30.0	34.0
3	31.942824264121995	33.0	31.0	34.0	30.0	34.0
4	35.5917966594436	37.0	35.0	37.0	33.0	37.0
5	35.43238401593561	37.0	35.0	37.0	33.0	37.0
6	35.37137511944979	37.0	35.0	37.0	33.0	37.0
7	35.65925651758435	37.0	35.0	37.0	35.0	37.0
8	35.50813066124712	37.0	35.0	37.0	33.0	37.0
9	37.28243314176503	39.0	37.0	39.0	34.0	39.0
10	36.70794088749512	39.0	37.0	39.0	32.0	39.0
11	36.64216611259909	39.0	35.0	39.0	32.0	39.0
12	36.522091818194056	39.0	35.0	39.0	32.0	39.0
13	36.38243448767817	39.0	35.0	39.0	32.0	39.0
14	37.474804506117174	39.0	36.0	41.0	32.0	41.0
15	37.60483317406695	39.0	36.0	41.0	33.0	41.0
16	37.633884709080874	39.0	36.0	41.0	33.0	41.0
17	37.576566306410584	39.0	36.0	41.0	33.0	41.0
18	37.559265938976296	39.0	36.0	41.0	32.0	41.0
19	37.5022221025855	39.0	36.0	41.0	32.0	41.0
20	37.40538230662593	39.0	35.0	41.0	32.0	41.0
21	37.3031319398646	39.0	35.0	41.0	32.0	41.0
22	37.25270730427058	39.0	35.0	41.0	32.0	41.0
23	36.771578352333144	39.0	35.0	41.0	31.0	41.0
24	36.87062006218119	39.0	35.0	41.0	31.0	41.0
25	36.850744962920096	39.0	35.0	41.0	31.0	41.0
26	36.88687196328349	39.0	35.0	41.0	31.0	41.0
27	34.503733563035844	38.0	35.0	40.0	23.0	41.0
28	35.350965692674194	38.0	35.0	40.0	23.0	41.0
29	36.022476749350595	38.0	34.0	40.0	28.0	41.0
30	36.32047941425861	38.0	35.0	40.0	30.0	41.0
31	36.36231981587908	38.0	35.0	40.0	30.0	41.0
32	36.13430463397892	38.0	35.0	40.0	30.0	41.0
33	36.18404823752675	38.0	35.0	40.0	30.0	41.0
34	36.10665419453829	38.0	35.0	40.0	30.0	41.0
35	36.035511918060806	38.0	35.0	40.0	29.0	41.0
36	35.94168023795744	38.0	35.0	40.0	29.0	41.0
37	35.829587208441566	38.0	35.0	40.0	29.0	41.0
38	35.72384688892179	38.0	34.0	40.0	28.0	41.0
39	35.61755205319049	38.0	34.0	40.0	28.0	41.0
40	35.49320852232197	38.0	34.0	40.0	27.0	41.0
41	35.36560922758045	38.0	34.0	40.0	27.0	41.0
42	35.26771288981009	38.0	34.0	40.0	27.0	41.0
43	35.123868423531945	37.0	34.0	40.0	26.0	41.0
44	35.010794223340824	37.0	34.0	40.0	26.0	41.0
45	34.93740427192829	37.0	34.0	40.0	26.0	41.0
46	34.78261618595136	37.0	33.0	40.0	26.0	41.0
47	34.645614342050365	37.0	33.0	40.0	25.0	41.0
48	34.55459696631179	37.0	33.0	40.0	25.0	41.0
49	34.44716079624221	36.0	33.0	40.0	25.0	41.0
50	34.329165937630385	36.0	33.0	40.0	24.0	41.0
51	32.73010673091159	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	7.0
12	5.0
13	17.0
14	27.0
15	76.0
16	120.0
17	219.0
18	465.0
19	761.0
20	1204.0
21	1880.0
22	2687.0
23	3834.0
24	5004.0
25	6526.0
26	8324.0
27	10278.0
28	12187.0
29	14988.0
30	18464.0
31	23069.0
32	29054.0
33	38850.0
34	57649.0
35	69025.0
36	71176.0
37	94732.0
38	128719.0
39	143606.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.142034213111884	18.368618689349788	24.120647653400447	14.368699444137874
2	30.420194080674033	25.35511918060808	26.2925476789728	17.932139059745083
3	32.19478054886338	23.977577087174794	27.4252681731921	16.402374190769727
4	28.08786120943754	26.846525525242598	25.70680628272251	19.358806982597343
5	24.386330906203312	31.31253448902408	24.5793348497288	19.721799755043808
6	24.343665459831225	37.61423437731329	23.518486116906015	14.523614045949474
7	81.53393719969313	6.858369560828543	7.423787668743858	4.183905570734465
8	83.2318066192008	5.9918706846660115	6.3586320138898245	4.417690682243368
9	77.60992745528203	7.995396977079099	8.779122195453505	5.615553372185359
10	41.98966338712499	25.060498795407742	16.262264633440555	16.687573184026704
11	32.03071373773537	25.942071898679657	21.92479037402926	20.102423989555714
12	27.64532497072639	23.01726806551905	28.629725837494448	20.70768112626011
13	25.699672943108254	24.070175910846714	29.440100135937225	20.790051010107806
14	22.595593480396776	26.799283974212308	27.71679295818248	22.888329587208442
15	22.13111885758893	25.103298833093312	30.652767870361647	22.11281443895611
16	24.152007429440502	23.456574112706765	29.402683750790725	22.988734707062005
17	23.689820858961763	23.581071077672647	27.309788826229152	25.419319237136435
18	24.413114577585162	24.689565135466157	28.05394419844143	22.84337608850725
19	25.629550868786925	26.30479548849917	25.3256436829567	22.7400099597572
20	26.082450638635784	25.372616051360048	25.934669376438446	22.610263933565726
21	25.704787413020362	25.848934709753834	26.275185399534312	22.171092477691488
22	24.29507799566616	25.217566858234967	25.57315710844022	24.91419803765865
23	23.209195278536722	27.1068251255064	25.158615862932205	24.525363733024673
24	22.995195090108883	25.58109799593534	27.121360987361875	24.302345926593897
25	23.296275858356104	26.453115115950414	26.04072733145803	24.209881694235456
26	22.465847454205306	26.136152572712955	25.946648003337863	25.451351969743875
27	22.23892649968371	30.327595257002116	24.612040538903617	22.821437704410556
28	22.53596952852663	26.76455941533533	26.991076595916503	23.708394460221538
29	23.23140284526037	26.652310259895827	26.362265979353694	23.754020915490116
30	24.878935113527774	26.314351471756016	25.533856444905044	23.27285696981117
31	25.887831599348576	26.91960860846041	23.508122585768316	23.684437206422697
32	25.19360960443613	27.053930739310083	23.835987025397383	23.916472630856404
33	25.82067053392374	25.868181267580994	23.75455928074402	24.55658891775125
34	22.72547409790172	27.461877010457748	25.167902663562092	24.64474622807844
35	24.388618958532415	24.743940026110714	25.554583507180446	25.31285750817642
36	25.533856444905044	26.862272708919367	24.405577464030472	23.198293382145117
37	24.275831437839003	28.392441351835153	25.233448633225215	22.098278577100633
38	23.961695312184553	26.98380866498876	26.219060821814562	22.835435201012128
39	24.14850805529011	26.183932488997158	25.646778556911936	24.02078089880079
40	24.033297890954117	25.913538540222614	26.902784694275834	23.150378874547435
41	22.60326518526494	25.275441123029918	25.791464218899314	26.329829472805827
42	23.557248415187285	25.893753617141552	25.490518041965572	25.058479925705594
43	24.109341983068415	23.50341188979663	27.566858234969516	24.820387892165442
44	23.641637168737127	24.843133824142992	26.928087861209438	24.587141145910444
45	23.690897589469575	24.561568796349885	26.318792985100742	25.428740629079797
46	23.92979717089059	26.253919972005008	26.18675890658017	23.629523950524234
47	22.811477947213287	25.48822998963647	27.37439265669794	24.325899406452308
48	23.47689740104174	25.28580465416762	26.703858732957375	24.53343921183327
49	23.095465618648973	24.92967603870846	27.45285939245481	24.521998950187754
50	22.922919554771934	24.66843429925033	27.591892219276172	24.81675392670157
51	23.09788826229155	24.40961520343477	25.734262910671745	26.758233623601935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2946.0
1	2818.5
2	2691.0
3	2884.5
4	3078.0
5	2368.0
6	1658.0
7	1685.5
8	1713.0
9	1703.5
10	1694.0
11	1752.5
12	1811.0
13	1811.0
14	1811.0
15	1852.5
16	1894.0
17	1804.5
18	1715.0
19	1717.0
20	1719.0
21	2083.0
22	2447.0
23	2290.0
24	2133.0
25	3181.0
26	4658.5
27	5088.0
28	6086.0
29	7084.0
30	8790.5
31	10497.0
32	11726.5
33	12956.0
34	15379.5
35	17803.0
36	18634.0
37	19465.0
38	21308.5
39	23152.0
40	25447.5
41	27743.0
42	30890.0
43	34037.0
44	39277.0
45	44517.0
46	48533.0
47	52549.0
48	56616.5
49	60684.0
50	60133.5
51	59583.0
52	56074.0
53	52565.0
54	47827.0
55	43089.0
56	40696.0
57	38303.0
58	37096.5
59	35890.0
60	34418.5
61	32947.0
62	31740.0
63	30533.0
64	27655.0
65	24777.0
66	22030.0
67	19283.0
68	17684.5
69	16086.0
70	15006.5
71	13927.0
72	12562.0
73	11197.0
74	9426.5
75	5817.5
76	3979.0
77	3284.5
78	2590.0
79	2187.0
80	1784.0
81	1220.5
82	657.0
83	518.0
84	379.0
85	371.0
86	363.0
87	272.0
88	181.0
89	123.0
90	65.0
91	45.5
92	26.0
93	18.0
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.415409715174945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98970335505643	23.416733713938207
2	9.15358464138683	5.568200544606264
3	3.2640033134523656	2.9782799427102686
4	1.673821070024784	2.0363981453878535
5	1.0295406494871375	1.5656950336289301
6	0.7209138265104823	1.3156133641590522
7	0.5001250500457629	1.0648055814174975
8	0.4194193419895822	1.0205448903265777
9	0.3417942587661734	0.9356231176800922
>10	4.8957289442582415	37.01774828910299
>50	0.9316927571791707	18.212741429784003
>100	0.073142235134581	3.3054606837906397
>500	0.005224445366755786	1.0872982002485756
>1k	0.0013061113416889464	0.4748570632190223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1353	0.18210204713387798	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1131	0.1522227755420665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1102	0.1483196274512443	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	892	0.12005545162115237	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	835	0.11238374675298457	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	822	0.1106340596777884	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	815	0.109691920483452	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.691826269532564E-4	0.0	0.0	0.12072840818853552	0.0
2	2.691826269532564E-4	0.0	0.0	0.5395765757278025	0.0
3	2.691826269532564E-4	0.0	0.0	0.7099691785892138	0.0
4	2.691826269532564E-4	0.0	0.0	0.9402549159477247	0.0
5	2.691826269532564E-4	0.0	0.0	1.5831976204255778	0.0
6	2.691826269532564E-4	0.0	0.0	2.1719000255723495	0.0
7	2.691826269532564E-4	0.0	0.0	2.669753294122397	0.0
8	2.691826269532564E-4	0.0	0.0	3.603547827023244	0.0
9	2.691826269532564E-4	0.0	0.0	4.027106690534193	0.0
10	2.691826269532564E-4	0.0	0.0	4.98082073782958	0.0
11	2.691826269532564E-4	0.0	0.0	6.081643090754922	0.0
12	2.691826269532564E-4	0.0	0.0	6.955409897845193	0.0
13	4.0377394042988464E-4	0.0	0.0	7.2921573641637165	0.0
14	4.0377394042988464E-4	0.0	0.0	7.427690816834682	0.0
15	4.0377394042988464E-4	0.0	0.0	7.664302345926594	0.0
16	4.0377394042988464E-4	0.0	0.0	8.1566373706241	0.0
17	4.0377394042988464E-4	0.0	0.0	8.70509697304136	0.0
18	4.0377394042988464E-4	0.0	0.0	9.354634651879568	0.0
19	4.0377394042988464E-4	0.0	0.0	9.63916068856916	0.0
20	4.0377394042988464E-4	0.0	0.0	9.935261578217743	0.0
21	4.0377394042988464E-4	0.0	0.0	10.26824048775892	0.0
22	4.0377394042988464E-4	0.0	0.0	10.611582928437798	0.0
23	4.0377394042988464E-4	0.0	0.0	10.972422239868639	0.0
24	4.0377394042988464E-4	0.0	0.0	11.2916728354352	0.0
25	4.0377394042988464E-4	0.0	0.0	11.561393827642364	0.0
26	4.0377394042988464E-4	0.0	0.0	11.810522348887602	0.0
27	4.0377394042988464E-4	0.0	0.0	12.071898679659215	0.0
28	5.383652539065129E-4	0.0	0.0	12.34754168965935	0.0
29	5.383652539065129E-4	0.0	0.0	12.632606091602847	0.0
30	5.383652539065129E-4	0.0	0.0	12.939608877643037	0.0
31	5.383652539065129E-4	0.0	0.0	13.235844358605096	0.0
32	6.729565673831411E-4	0.0	0.0	13.544866014347434	0.0
33	6.729565673831411E-4	0.0	0.0	13.839486399547773	0.0
34	6.729565673831411E-4	0.0	0.0	14.147296733468822	0.0
35	6.729565673831411E-4	0.0	0.0	14.471258025007065	0.0
36	6.729565673831411E-4	0.0	0.0	14.757668340085331	0.0
37	6.729565673831411E-4	0.0	0.0	15.066959178454622	0.0
38	6.729565673831411E-4	0.0	0.0	15.366963216194026	0.0
39	6.729565673831411E-4	0.0	0.0	15.666563479993002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	20	7.031674E-4	45.0	1
CGCAATA	20	7.031674E-4	45.0	2
ATACGCG	20	7.031674E-4	45.0	1
CGATCTA	20	7.031674E-4	45.0	10
TGTCGGT	25	3.8894686E-5	45.0	37
CCGCATT	20	7.031674E-4	45.0	23
GCACTAG	20	7.031674E-4	45.0	1
CACTATC	30	2.164461E-6	44.999996	29
TATAGCG	30	2.164461E-6	44.999996	1
ATTGGGT	80	0.0	42.1875	4
CGACGGT	55	6.002665E-11	40.909092	28
CGTAAGG	210	0.0	40.714287	2
GCGATCT	50	1.0804797E-9	40.5	9
ACGTAAG	50	1.0804797E-9	40.5	1
ATACGCA	50	1.0804797E-9	40.5	27
AATACGC	50	1.0804797E-9	40.5	26
GTTAGCG	45	1.9266736E-8	40.0	1
TCGTAAC	130	0.0	39.807693	34
CGTAACC	130	0.0	39.807693	35
TCGATAG	40	3.4560253E-7	39.375	1
>>END_MODULE