SRR3553982_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1097296
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	5344	0.4870153541068226	RNA PCR Primer, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4880	0.4447295898280865	RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	3433	0.31285997579504526	TruSeq Adapter, Index 10 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2687	0.24487467374345664	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1927	0.17561350811449236	RNA PCR Primer, Index 35 (95% over 23bp)
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1675	0.1526479637217305	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1673	0.15246569749639113	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1457	0.13278094515973812	TruSeq Adapter, Index 10 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1121	0.10216021930272233	RNA PCR Primer, Index 10 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1099	0.10015529082398915	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	35	1.2118653E-7	45.000004	38
AACCGCA	20	7.033145E-4	45.000004	10
ACGTTAG	40	6.8139343E-9	45.000004	1
TACGAAC	20	7.033145E-4	45.000004	23
ACTACGT	20	7.033145E-4	45.000004	1
CGAATTG	20	7.033145E-4	45.000004	2
TATTACG	20	7.033145E-4	45.000004	1
GAACGTA	20	7.033145E-4	45.000004	9
TTACGAG	20	7.033145E-4	45.000004	1
TAGTCGT	20	7.033145E-4	45.000004	35
GTACGAG	35	1.2118653E-7	45.000004	1
GACTAAC	20	7.033145E-4	45.000004	9
TACCCGT	25	3.8906895E-5	45.0	34
CTATCGG	25	3.8906895E-5	45.0	2
ACGATAG	25	3.8906895E-5	45.0	1
ATGCGTT	45	3.8562575E-10	45.0	31
ATAGTCG	25	3.8906895E-5	45.0	1
TGTACGA	25	3.8906895E-5	45.0	14
CGTCCAA	45	3.8562575E-10	45.0	38
TATCGAT	25	3.8906895E-5	45.0	24