SRR3553981_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029189
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	5008	0.48659672810339016	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	4859	0.4721193094757134	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	3707	0.3601865157905885	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2582	0.2508771469574588	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.1648871101420633	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1448	0.14069330317366394	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1211	0.11766546280615126	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1191	0.11572218513800674	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	1149	0.1116413020349032	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1123	0.10911504106631531	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1068	0.10377102747891787	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGT	20	7.0329406E-4	45.000004	29
ATATACG	20	7.0329406E-4	45.000004	1
CGTAGAC	20	7.0329406E-4	45.000004	29
TCCCGTA	20	7.0329406E-4	45.000004	37
ACCGTTG	20	7.0329406E-4	45.000004	10
CACGTTA	20	7.0329406E-4	45.000004	18
CACGTAG	20	7.0329406E-4	45.000004	1
TAGGACG	40	6.8139343E-9	45.000004	1
TTCGCAC	35	1.2117562E-7	45.000004	22
CGTTTAC	40	6.8139343E-9	45.000004	33
CGCTTAG	20	7.0329406E-4	45.000004	27
GACCGTT	25	3.89052E-5	45.0	9
CGTGTTA	25	3.89052E-5	45.0	12
GTCGAAT	95	0.0	42.63158	43
CTACGAA	180	0.0	41.25	11
CGTAAGG	165	0.0	40.90909	2
GTTAACG	90	0.0	40.0	1
CGAATAT	180	0.0	40.0	14
ACGTAAG	45	1.9279469E-8	40.0	1
CGCACGG	125	0.0	39.6	2