##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029189
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73417418958034	31.0	31.0	34.0	30.0	34.0
2	31.851574394984787	31.0	31.0	34.0	30.0	34.0
3	31.844733085954086	33.0	31.0	34.0	30.0	34.0
4	35.51925933914956	37.0	35.0	37.0	33.0	37.0
5	35.35057117788861	37.0	35.0	37.0	33.0	37.0
6	35.292395274337366	37.0	35.0	37.0	32.0	37.0
7	35.623102267902205	37.0	35.0	37.0	35.0	37.0
8	35.440036766813485	37.0	35.0	37.0	33.0	37.0
9	37.20824552147371	39.0	37.0	39.0	34.0	39.0
10	36.64728247192693	39.0	35.0	39.0	32.0	39.0
11	36.57561924971993	39.0	35.0	39.0	32.0	39.0
12	36.47770623277163	38.0	35.0	39.0	32.0	39.0
13	36.35468218179557	38.0	35.0	39.0	32.0	39.0
14	37.31586521037438	39.0	36.0	41.0	32.0	41.0
15	37.47668212544052	39.0	36.0	41.0	32.0	41.0
16	37.54929269551074	39.0	36.0	41.0	32.0	41.0
17	37.48089223650855	39.0	36.0	41.0	32.0	41.0
18	37.48934355108731	39.0	36.0	41.0	32.0	41.0
19	37.421620324352475	39.0	36.0	41.0	32.0	41.0
20	37.26584621483518	39.0	35.0	41.0	32.0	41.0
21	37.210263615332074	39.0	35.0	41.0	32.0	41.0
22	37.2431331854499	39.0	35.0	41.0	32.0	41.0
23	36.73999819275177	39.0	35.0	41.0	31.0	41.0
24	36.86584776945731	39.0	35.0	41.0	31.0	41.0
25	36.89183522171341	39.0	35.0	41.0	31.0	41.0
26	36.85797749490133	39.0	35.0	40.0	31.0	41.0
27	34.53323442050002	38.0	35.0	40.0	23.0	41.0
28	35.35675760234515	38.0	34.0	40.0	23.0	41.0
29	36.020124583531306	38.0	34.0	40.0	28.0	41.0
30	36.29897035432754	38.0	35.0	40.0	30.0	41.0
31	36.38127496504529	38.0	35.0	40.0	30.0	41.0
32	36.11949991692488	38.0	35.0	40.0	30.0	41.0
33	36.09608536430141	38.0	35.0	40.0	29.0	41.0
34	36.02031210982628	38.0	35.0	40.0	29.0	41.0
35	35.9284679490356	38.0	35.0	40.0	29.0	41.0
36	35.812445527497864	38.0	35.0	40.0	28.0	41.0
37	35.648878874531306	38.0	34.0	40.0	27.0	41.0
38	35.539906664373596	38.0	34.0	40.0	27.0	41.0
39	35.394343507363565	38.0	34.0	40.0	27.0	41.0
40	35.26673817928486	38.0	34.0	40.0	26.0	41.0
41	35.141524054376795	38.0	34.0	40.0	26.0	41.0
42	35.043816053222486	38.0	34.0	40.0	26.0	41.0
43	34.93405875888685	38.0	34.0	40.0	25.0	41.0
44	34.834525048363325	37.0	34.0	40.0	24.0	41.0
45	34.82175479916711	37.0	34.0	40.0	25.0	41.0
46	34.74168981596189	37.0	33.0	40.0	24.0	41.0
47	34.560637550537365	37.0	33.0	40.0	24.0	41.0
48	34.4987509582788	37.0	33.0	40.0	24.0	41.0
49	34.43965491275169	37.0	33.0	40.0	24.0	41.0
50	34.302588737345616	37.0	33.0	40.0	24.0	41.0
51	32.60643186042603	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	5.0
11	5.0
12	4.0
13	20.0
14	35.0
15	74.0
16	149.0
17	299.0
18	600.0
19	1176.0
20	2093.0
21	3308.0
22	4793.0
23	6370.0
24	7970.0
25	9718.0
26	12275.0
27	14749.0
28	17548.0
29	21524.0
30	26232.0
31	32337.0
32	40744.0
33	53718.0
34	77126.0
35	89096.0
36	98618.0
37	131022.0
38	180228.0
39	197296.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.583939393056085	18.11105637545679	23.626564217068	13.678440014419122
2	31.27073841636473	26.900501268474496	24.823526096761626	17.00523421839915
3	31.338753134749787	26.306829940856346	26.204516371628532	16.149900552765335
4	27.69743944018057	25.97841601493992	27.57180653893502	18.752338005944484
5	26.339088350147545	29.394309500004372	24.541070687696816	19.725531462151267
6	24.10169560692934	37.93540350703321	23.99199758256258	13.970903303474872
7	79.24870942071864	6.126571504359258	10.092704061158834	4.532015013763264
8	80.40593127209871	7.126776520153247	7.702375365457656	4.764916842290385
9	74.44599582778285	7.6276563391174985	9.898862113761417	8.027485719338236
10	40.48751006860742	24.957903747513818	17.4980494350406	17.056536748838163
11	31.815827802279273	25.30963700544798	23.376755872828024	19.497779319444728
12	26.78963727750685	22.287451575949607	29.17267868195249	21.75023246459105
13	26.296919224748805	23.102656557736235	31.276568249369163	19.323855968145793
14	21.726913132573316	27.845031379076147	29.218442871037293	21.209612617313244
15	20.50944967348077	24.646590665077063	33.32643469761142	21.517524963830745
16	23.28153526708894	24.263667800569184	29.238264303252365	23.216532629089507
17	23.560298448584273	24.479663113383452	27.497573332011903	24.46246510602037
18	24.64746514002773	23.399395057661906	28.767019468727316	23.186120333583045
19	24.661068083704745	26.10803263540516	25.77485767920178	23.45604160168832
20	25.32566904621017	25.846078805739275	27.331131599735325	21.497120548315227
21	24.124820611180258	27.637392160234903	27.788190507282916	20.44959672130192
22	22.889673325307598	24.07390673627487	27.74951928168684	25.286900656730687
23	22.12557654619317	27.588615890764473	26.77564567829621	23.510161884746143
24	22.13432129569982	25.73278571768645	27.905661642322254	24.227231344291475
25	22.854305671747365	27.721244591615342	25.39047735644279	24.033972380194502
26	21.508100067140244	25.63737078418055	28.229703193485356	24.624825955193845
27	22.1408312758881	30.076691453173325	25.661467427265546	22.12100984367303
28	21.42531643847729	26.2242406399602	28.828912862457724	23.52153005910479
29	22.990529436284298	24.98627560146873	28.260698472292262	23.762496489954714
30	23.5351330027818	25.432354990191307	28.469795149384613	22.56271685764228
31	24.443906804289593	25.92128365149647	26.10356309676843	23.531246447445515
32	25.70043014451184	25.711701154987082	26.788568474789372	21.7993002257117
33	24.59179023483539	24.370936727850765	25.685272578700317	25.352000458613528
34	23.565933953821894	24.85869942255504	27.9600734170303	23.615293206592764
35	24.068562722687474	24.22538523050674	28.016428469406495	23.689623577399292
36	25.933623464689187	26.25028056071334	25.70315073324725	22.11294524135023
37	24.926616977056693	25.910692788205083	27.754377475857204	21.40831275888102
38	25.34976568929516	25.456062977742672	25.71121533557005	23.48295599739212
39	24.611223011516834	25.00026720067937	26.092194922409778	24.296314865394013
40	25.377360232182816	23.630645100171105	28.044411667827774	22.9475829998183
41	23.84110207163116	25.251824494820678	26.123481692866907	24.783591740681253
42	25.131729934929346	23.97907478606942	28.673547812889566	22.215647466111665
43	24.5784787828086	23.055726402050546	28.952602486035122	23.413192329105733
44	24.288930410255066	23.569043198090924	27.7933401931035	24.34868619855051
45	23.50122280747268	22.92037711246428	27.684905299221036	25.893494780842
46	24.732094882475426	24.331391027304022	26.644280107929642	24.292233982290913
47	22.528418006799527	24.777567579910006	30.13498978321766	22.559024630072805
48	23.14395120818431	25.139988865018964	27.414012392281688	24.30204753451504
49	24.496958284629937	23.120631876166577	28.971355115532717	23.411054723670773
50	22.813399676832923	22.99315286113629	29.200370388723552	24.993077073307234
51	22.710794615954892	22.760251032609172	27.158082723387057	27.37087162804888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3155.0
1	3070.0
2	2985.0
3	3552.5
4	4120.0
5	3123.0
6	2126.0
7	2144.5
8	2163.0
9	2268.5
10	2374.0
11	2373.0
12	2372.0
13	2448.0
14	2524.0
15	2393.5
16	2263.0
17	2382.5
18	2502.0
19	2483.0
20	2464.0
21	2733.0
22	3002.0
23	3042.0
24	3082.0
25	4451.0
26	6192.0
27	6564.0
28	8426.0
29	10288.0
30	11577.0
31	12866.0
32	16396.0
33	19926.0
34	22096.0
35	24266.0
36	25189.0
37	26112.0
38	28264.5
39	30417.0
40	34341.5
41	38266.0
42	42707.5
43	47149.0
44	53299.5
45	59450.0
46	77699.0
47	95948.0
48	95647.5
49	95347.0
50	96020.5
51	96694.0
52	86760.5
53	76827.0
54	69104.0
55	61381.0
56	58123.0
57	54865.0
58	50964.0
59	47063.0
60	43953.5
61	40844.0
62	38351.5
63	35859.0
64	32066.5
65	28274.0
66	24704.5
67	21135.0
68	18962.0
69	16789.0
70	14981.0
71	13173.0
72	11616.0
73	10059.0
74	8626.0
75	5967.0
76	4741.0
77	3983.5
78	3226.0
79	2445.5
80	1665.0
81	1355.5
82	1046.0
83	719.0
84	392.0
85	307.5
86	223.0
87	142.5
88	62.0
89	71.0
90	80.0
91	51.5
92	23.0
93	18.0
94	13.0
95	9.5
96	6.0
97	3.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1029189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.16613408553485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88486290308994	24.409226752032204
2	9.815945071605736	6.314820106990301
3	3.772465910371255	3.6403693291833323
4	1.9199537401043367	2.470299577688809
5	1.1665962409642396	1.876244555526833
6	0.7972219119724523	1.5386128149859417
7	0.5788035501844064	1.3032510823090653
8	0.4333764583941511	1.1152036216176384
9	0.36854249047725857	1.0669128448417604
>10	4.4329798599447665	33.97869246152374
>50	0.760398204290204	15.933982374418864
>100	0.0625395676378958	3.1912796603385836
>500	0.0027060389843040888	0.6403449595708345
>1k	0.003307380980816109	2.0364149628037294
>5k	3.0067099825600987E-4	0.4843448961683642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	5008	0.48659672810339016	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	4859	0.4721193094757134	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	3707	0.3601865157905885	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2582	0.2508771469574588	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.1648871101420633	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1448	0.14069330317366394	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1211	0.11766546280615126	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1191	0.11572218513800674	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	1149	0.1116413020349032	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1123	0.10911504106631531	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1068	0.10377102747891787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.886555336289059E-4	0.0	0.0	0.12806199833072449	0.0
2	3.886555336289059E-4	0.0	0.0	0.6707222871600843	0.0
3	3.886555336289059E-4	0.0	0.0	0.911688718010006	0.0
4	3.886555336289059E-4	0.0	0.0	1.3252182057911617	0.0
5	3.886555336289059E-4	0.0	0.0	2.7393413648999356	0.0
6	3.886555336289059E-4	0.0	0.0	3.664924518237175	0.0
7	3.886555336289059E-4	0.0	0.0	4.468858489548567	0.0
8	3.886555336289059E-4	0.0	0.0	5.747243703537445	0.0
9	3.886555336289059E-4	0.0	0.0	6.291069958967692	0.0
10	3.886555336289059E-4	0.0	0.0	7.927601247195607	0.0
11	3.886555336289059E-4	0.0	0.0	9.304219147309192	0.0
12	3.886555336289059E-4	0.0	0.0	10.751183699009609	0.0
13	3.886555336289059E-4	0.0	0.0	11.240403851964993	0.0
14	3.886555336289059E-4	0.0	0.0	11.450860823425046	0.0
15	3.886555336289059E-4	0.0	0.0	11.831063099197523	0.0
16	3.886555336289059E-4	0.0	0.0	12.505574777810489	0.0
17	3.886555336289059E-4	0.0	0.0	13.199616396988308	0.0
18	3.886555336289059E-4	0.0	0.0	14.071079267267722	0.0
19	3.886555336289059E-4	0.0	0.0	14.612670753379604	9.716388340722647E-5
20	3.886555336289059E-4	0.0	0.0	15.0590416337524	9.716388340722647E-5
21	3.886555336289059E-4	0.0	0.0	15.559629961066431	9.716388340722647E-5
22	3.886555336289059E-4	0.0	0.0	16.10335905261327	9.716388340722647E-5
23	3.886555336289059E-4	0.0	0.0	16.606084985362262	9.716388340722647E-5
24	3.886555336289059E-4	0.0	0.0	17.006497348883443	9.716388340722647E-5
25	3.886555336289059E-4	0.0	0.0	17.343170204889482	9.716388340722647E-5
26	3.886555336289059E-4	0.0	0.0	17.684604091182475	9.716388340722647E-5
27	5.829833004433588E-4	0.0	0.0	18.08909733780676	9.716388340722647E-5
28	6.801471838505852E-4	0.0	0.0	18.457056964269924	9.716388340722647E-5
29	6.801471838505852E-4	0.0	0.0	18.840951467611877	9.716388340722647E-5
30	6.801471838505852E-4	0.0	0.0	19.325119098630086	9.716388340722647E-5
31	7.773110672578118E-4	0.0	0.0	19.710956879640182	9.716388340722647E-5
32	7.773110672578118E-4	0.0	0.0	20.107191196174853	9.716388340722647E-5
33	7.773110672578118E-4	0.0	0.0	20.496624040871016	9.716388340722647E-5
34	7.773110672578118E-4	0.0	0.0	20.870704991988838	9.716388340722647E-5
35	7.773110672578118E-4	0.0	0.0	21.28190254656822	9.716388340722647E-5
36	7.773110672578118E-4	0.0	0.0	21.664242427775655	9.716388340722647E-5
37	8.744749506650382E-4	0.0	0.0	22.045416342382207	9.716388340722647E-5
38	8.744749506650382E-4	0.0	0.0	22.44845213075538	9.716388340722647E-5
39	8.744749506650382E-4	0.0	0.0	22.974594559405514	9.716388340722647E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGT	20	7.0329406E-4	45.000004	29
ATATACG	20	7.0329406E-4	45.000004	1
CGTAGAC	20	7.0329406E-4	45.000004	29
TCCCGTA	20	7.0329406E-4	45.000004	37
ACCGTTG	20	7.0329406E-4	45.000004	10
CACGTTA	20	7.0329406E-4	45.000004	18
CACGTAG	20	7.0329406E-4	45.000004	1
TAGGACG	40	6.8139343E-9	45.000004	1
TTCGCAC	35	1.2117562E-7	45.000004	22
CGTTTAC	40	6.8139343E-9	45.000004	33
CGCTTAG	20	7.0329406E-4	45.000004	27
GACCGTT	25	3.89052E-5	45.0	9
CGTGTTA	25	3.89052E-5	45.0	12
GTCGAAT	95	0.0	42.63158	43
CTACGAA	180	0.0	41.25	11
CGTAAGG	165	0.0	40.90909	2
GTTAACG	90	0.0	40.0	1
CGAATAT	180	0.0	40.0	14
ACGTAAG	45	1.9279469E-8	40.0	1
CGCACGG	125	0.0	39.6	2
>>END_MODULE