SRR3553979_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623885
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1860	0.2981318672511761	RNA PCR Primer, Index 23 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1781	0.28546927719050785	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1399	0.22424004423892224	RNA PCR Primer, Index 30 (95% over 23bp)
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1108	0.17759683274962534	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	1074	0.17214711044503395	RNA PCR Primer, Index 30 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	991	0.15884337658382555	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	892	0.1429750675204565	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	864	0.13848706091667537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	797	0.1277479022576276	No Hit
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	744	0.11925274690047043	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.11236045104466369	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTCG	20	7.0308044E-4	45.0	28
TATGGAT	20	7.0308044E-4	45.0	36
TCCCTAT	35	1.2107375E-7	45.0	15
ATTCGAA	20	7.0308044E-4	45.0	34
CTAAGAT	20	7.0308044E-4	45.0	12
ACTTACG	25	3.888748E-5	45.0	26
GTTACTA	20	7.0308044E-4	45.0	31
TCTACGG	20	7.0308044E-4	45.0	2
TACGAGA	40	6.8066583E-9	45.0	24
TCTAACG	40	6.8066583E-9	45.0	24
TCGCTAG	25	3.888748E-5	45.0	15
GTATCGC	20	7.0308044E-4	45.0	1
CGTACGA	20	7.0308044E-4	45.0	2
CGTGTCA	20	7.0308044E-4	45.0	19
ACCGTTG	20	7.0308044E-4	45.0	27
CAGTACG	20	7.0308044E-4	45.0	1
TCAACGG	25	3.888748E-5	45.0	2
GACTAGT	20	7.0308044E-4	45.0	11
TTAGCGG	70	0.0	41.785713	2
TAAGACG	55	6.002665E-11	40.909092	1