##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623885
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.666497832132524	31.0	31.0	34.0	30.0	34.0
2	31.77574072144706	31.0	31.0	34.0	30.0	34.0
3	31.786322799874977	31.0	31.0	34.0	30.0	34.0
4	35.454117345344095	37.0	35.0	37.0	33.0	37.0
5	35.29813827868918	37.0	35.0	37.0	33.0	37.0
6	35.253735864782776	37.0	35.0	37.0	32.0	37.0
7	35.57646200822267	37.0	35.0	37.0	35.0	37.0
8	35.4147158530819	37.0	35.0	37.0	33.0	37.0
9	37.16117874287729	39.0	37.0	39.0	34.0	39.0
10	36.60636655793937	38.0	35.0	39.0	32.0	39.0
11	36.54058360114444	38.0	35.0	39.0	32.0	39.0
12	36.48430239547353	38.0	35.0	39.0	32.0	39.0
13	36.33557145948372	38.0	35.0	39.0	32.0	39.0
14	37.3826698830714	39.0	36.0	41.0	32.0	41.0
15	37.52684388949886	39.0	36.0	41.0	32.0	41.0
16	37.568984668648866	39.0	36.0	41.0	32.0	41.0
17	37.51717383812722	39.0	36.0	41.0	32.0	41.0
18	37.48839770149947	39.0	36.0	41.0	32.0	41.0
19	37.43109387146669	39.0	36.0	41.0	32.0	41.0
20	37.320470920121494	39.0	35.0	41.0	32.0	41.0
21	37.228208724364265	39.0	35.0	40.0	32.0	41.0
22	37.222911273712306	39.0	35.0	41.0	32.0	41.0
23	36.75344975436178	39.0	35.0	40.0	31.0	41.0
24	36.83914503474198	39.0	35.0	40.0	31.0	41.0
25	36.83096403984709	39.0	35.0	40.0	31.0	41.0
26	36.88292393630236	39.0	35.0	40.0	31.0	41.0
27	34.59792269408625	38.0	35.0	40.0	24.0	41.0
28	35.39998717712399	38.0	35.0	40.0	23.0	41.0
29	36.048715708824545	38.0	35.0	40.0	28.0	41.0
30	36.314795194627216	38.0	35.0	40.0	30.0	41.0
31	36.35502376239211	38.0	35.0	40.0	30.0	41.0
32	36.15267076464413	38.0	35.0	40.0	30.0	41.0
33	36.16217091290863	38.0	35.0	40.0	30.0	41.0
34	36.11436402542135	38.0	35.0	40.0	30.0	41.0
35	36.05615137405131	38.0	35.0	40.0	30.0	41.0
36	35.94497543617814	38.0	35.0	40.0	29.0	41.0
37	35.87530714795194	38.0	35.0	40.0	29.0	41.0
38	35.80873718714186	38.0	35.0	40.0	29.0	41.0
39	35.70508988034654	38.0	34.0	40.0	28.0	41.0
40	35.61331976245622	38.0	34.0	40.0	28.0	41.0
41	35.50173028683171	38.0	34.0	40.0	27.0	41.0
42	35.4567348149098	38.0	34.0	40.0	27.0	41.0
43	35.353366405667714	38.0	34.0	40.0	27.0	41.0
44	35.22220280981271	38.0	34.0	40.0	27.0	41.0
45	35.154132572509354	38.0	34.0	40.0	26.0	41.0
46	34.97451773964753	37.0	34.0	40.0	26.0	41.0
47	34.83141925194547	37.0	33.0	40.0	26.0	41.0
48	34.77497936318392	37.0	33.0	40.0	26.0	41.0
49	34.68725165695601	37.0	33.0	40.0	26.0	41.0
50	34.583048157913716	37.0	33.0	40.0	26.0	41.0
51	32.86747237070934	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	6.0
13	9.0
14	22.0
15	38.0
16	100.0
17	158.0
18	332.0
19	591.0
20	978.0
21	1525.0
22	2268.0
23	3068.0
24	4065.0
25	5222.0
26	6705.0
27	8192.0
28	10294.0
29	12446.0
30	15505.0
31	19589.0
32	25084.0
33	33384.0
34	47786.0
35	57127.0
36	60753.0
37	81823.0
38	111059.0
39	115727.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14181299438198	18.630035984195807	23.04399047901456	13.184160542407655
2	31.68757062599678	25.70024924465246	24.742220120695322	17.869960008655443
3	32.63037258469109	25.537398719315256	25.778789360218628	16.05343933577502
4	28.571291183471313	26.99103200108994	25.50630324498906	18.931373570449683
5	23.79749473059939	31.228351378859887	24.77908588922638	20.195068001314347
6	23.598099008631397	37.55852440754306	24.141147807688917	14.702228776136629
7	78.96182790097535	6.9641039614672575	9.22557843192255	4.848489705634853
8	79.38001394487766	6.566113947281951	8.404914367231141	5.648957740609247
9	73.1391201904197	8.44562699856544	11.187638747525586	7.227614063489265
10	38.39281277799594	27.292850445194226	18.03617653894548	16.278160237864352
11	30.287152279666923	26.847095217868677	23.540556352532917	19.32519614993148
12	26.530690752302107	23.12253059458073	29.501270266154823	20.84550838696234
13	24.23058736786427	25.78055250567012	31.064859709722143	18.924000416743468
14	20.32281590357197	28.13883969000697	29.500629122354283	22.037715284066774
15	19.776080527661346	25.99437396315026	33.283217259591105	20.946328249597283
16	20.885579874496102	25.393301650143858	31.365556152175483	22.35556232318456
17	21.26962501101966	25.287993780905133	27.71552449570033	25.72685671237488
18	22.201527525104787	25.158001875345615	29.251865327744696	23.3886052718049
19	23.386200982552875	27.09457672487718	26.824174326999366	22.695047965570577
20	24.16406869855823	26.630068041385833	27.018120326662764	22.187742933393174
21	23.408641015571778	28.20551864526315	27.289003582390986	21.096836756774085
22	21.847776433156753	26.510174150684822	25.677809211633555	25.964240204524874
23	21.172491725237823	28.742156006315266	26.11154299269898	23.973809275747936
24	21.79937007621597	25.83504972871603	28.378948043309265	23.986632151758737
25	21.3988154868285	27.774509725350022	26.38643339718057	24.440241390640903
26	20.859773836524358	27.465798985389934	26.475231813555382	25.199195364530325
27	21.108377345183808	31.407551071110863	25.163932455500614	22.320139128204715
28	20.784920297811297	26.84789664761935	28.1540668552698	24.21311619929955
29	23.13102574993789	25.200637938081538	26.238329179255793	25.43000713272478
30	25.178999334813305	26.14440161247666	25.027048254085287	23.649550798624748
31	24.6652828646305	27.681383588321566	23.744600367054826	23.908733179993106
32	25.36236646176779	26.801253436130057	24.391835033700122	23.44454506840203
33	25.720124702469207	26.454074068137558	24.239082523221427	23.586718706171812
34	22.694406821770038	26.52572188784792	26.64561577854893	24.13425551183311
35	24.666244580331313	25.172267324907637	25.617701980332914	24.54378611442814
36	25.25962316773123	27.515327343981667	25.706500396707728	21.518549091579377
37	23.89430744448095	28.213372656819768	27.170231693340916	20.72208820535836
38	24.101396892055426	27.759282560087193	26.45183006483567	21.68749048302171
39	24.627295094448495	26.509853578784554	25.75218189249621	23.11066943427074
40	24.3963230403039	25.03538312349231	28.731096275755945	21.837197560447837
41	20.6632632616588	27.254381817161814	27.26383868821978	24.8185162329596
42	21.883680485987	26.880274409546633	28.04282840587608	23.193216698590284
43	23.509621164156854	25.083949766383228	28.39690006972439	23.00952899973553
44	23.64874936887407	24.695256337305754	26.547520777066286	25.108473516753886
45	24.01660562443399	24.068858844178013	26.678955256177016	25.235580275210978
46	24.817714803208922	24.966299878984106	26.593041986904637	23.62294333090233
47	22.401564390873318	26.23255888505093	28.45957187622719	22.906304847848563
48	22.17620234498345	25.331591559341867	27.912836500316562	24.57936959535812
49	23.37658382554477	24.12527949862555	28.96928119765662	23.52885547817306
50	22.5003005361565	23.86096796685287	28.52512882983242	25.113602667158208
51	22.337289724869166	23.760789248018465	26.183992242160016	27.717928784952356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3762.0
1	3300.0
2	2838.0
3	2617.5
4	2397.0
5	1875.0
6	1353.0
7	1348.0
8	1343.0
9	1424.0
10	1505.0
11	1534.0
12	1563.0
13	1535.5
14	1508.0
15	1618.0
16	1728.0
17	1564.5
18	1401.0
19	1572.5
20	1744.0
21	1755.0
22	1766.0
23	1857.5
24	1949.0
25	2748.5
26	4465.0
27	5382.0
28	5647.5
29	5913.0
30	7741.0
31	9569.0
32	10834.5
33	12100.0
34	12726.5
35	13353.0
36	14428.5
37	15504.0
38	16205.0
39	16906.0
40	19857.5
41	22809.0
42	26434.0
43	30059.0
44	34931.0
45	39803.0
46	46955.0
47	54107.0
48	59421.0
49	64735.0
50	63592.0
51	62449.0
52	54335.0
53	46221.0
54	41443.0
55	36665.0
56	33256.0
57	29847.0
58	28606.5
59	27366.0
60	25532.5
61	23699.0
62	22849.0
63	21999.0
64	19149.0
65	16299.0
66	13513.5
67	10728.0
68	9740.0
69	8752.0
70	7937.0
71	7122.0
72	6237.0
73	5352.0
74	4334.0
75	2783.5
76	2251.0
77	1877.5
78	1504.0
79	1187.5
80	871.0
81	667.0
82	463.0
83	284.0
84	105.0
85	103.5
86	102.0
87	87.5
88	73.0
89	45.5
90	18.0
91	12.5
92	7.0
93	18.0
94	29.0
95	15.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.996734181432586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1355130689525	25.883587995721648
2	10.027435973388034	6.818001506172154
3	3.6346193394970348	3.706955626067243
4	1.7902913172519006	2.4345623207975864
5	1.0882961738856227	1.8499257867129824
6	0.7358876538339829	1.5010666172875198
7	0.5169002421802248	1.230104409220346
8	0.4441741993027129	1.208037774715599
9	0.3856131097075008	1.1798627748841355
>10	4.629801227279532	37.180859835108805
>50	0.5505534224718496	11.704617872698307
>100	0.05440435002401689	3.1047486530097013
>500	0.0041849500018474525	1.0559939410440329
>1k	0.002324972223248585	1.1416748865598811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1860	0.2981318672511761	RNA PCR Primer, Index 23 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1781	0.28546927719050785	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1399	0.22424004423892224	RNA PCR Primer, Index 30 (95% over 23bp)
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1108	0.17759683274962534	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	1074	0.17214711044503395	RNA PCR Primer, Index 30 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	991	0.15884337658382555	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	892	0.1429750675204565	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	864	0.13848706091667537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	797	0.1277479022576276	No Hit
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	744	0.11925274690047043	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.11236045104466369	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.602859501350409E-4	0.0	0.0	0.19186228231164398	0.0
2	1.602859501350409E-4	0.0	0.0	1.0011460445434655	0.0
3	1.602859501350409E-4	0.0	0.0	1.37076544555487	0.0
4	1.602859501350409E-4	0.0	0.0	1.8982665074492895	0.0
5	1.602859501350409E-4	0.0	0.0	3.3876435561040896	0.0
6	1.602859501350409E-4	0.0	0.0	4.5243915144618	0.0
7	1.602859501350409E-4	0.0	0.0	5.518645263149459	0.0
8	1.602859501350409E-4	0.0	0.0	7.208219463522925	0.0
9	1.602859501350409E-4	0.0	0.0	7.969257154764099	0.0
10	1.602859501350409E-4	0.0	0.0	9.645367335326222	0.0
11	1.602859501350409E-4	0.0	0.0	11.466055442910152	0.0
12	1.602859501350409E-4	0.0	0.0	12.92802359409186	0.0
13	1.602859501350409E-4	0.0	0.0	13.50168700962517	0.0
14	1.602859501350409E-4	0.0	0.0	13.71214246215248	0.0
15	1.602859501350409E-4	0.0	0.0	14.103721038332385	0.0
16	1.602859501350409E-4	0.0	0.0	14.942337129438918	0.0
17	1.602859501350409E-4	0.0	0.0	15.824070141131779	0.0
18	1.602859501350409E-4	0.0	0.0	16.941583785473284	0.0
19	1.602859501350409E-4	0.0	0.0	17.46988627711838	0.0
20	1.602859501350409E-4	0.0	0.0	17.979916170448078	0.0
21	1.602859501350409E-4	0.0	0.0	18.548290149626933	0.0
22	1.602859501350409E-4	0.0	0.0	19.118747846157547	0.0
23	1.602859501350409E-4	0.0	0.0	19.716614440161248	0.0
24	1.602859501350409E-4	0.0	0.0	20.201479439319748	0.0
25	1.602859501350409E-4	0.0	0.0	20.6289620683299	0.0
26	1.602859501350409E-4	0.0	0.0	21.001466616443736	0.0
27	1.602859501350409E-4	0.0	0.0	21.418690944645245	0.0
28	3.205719002700818E-4	0.0	0.0	21.825015828237575	0.0
29	3.205719002700818E-4	0.0	0.0	22.257787893602185	0.0
30	3.205719002700818E-4	0.0	0.0	22.738164886156905	0.0
31	3.205719002700818E-4	0.0	0.0	23.163082939964898	0.0
32	3.205719002700818E-4	0.0	0.0	23.613646745794497	0.0
33	3.205719002700818E-4	0.0	0.0	24.030069644245334	0.0
34	3.205719002700818E-4	0.0	0.0	24.431585949333613	0.0
35	3.205719002700818E-4	0.0	0.0	24.871090024603895	0.0
36	3.205719002700818E-4	0.0	0.0	25.26843889498866	0.0
37	3.205719002700818E-4	0.0	0.0	25.689670371943546	0.0
38	3.205719002700818E-4	0.0	0.0	26.07868437292129	0.0
39	3.205719002700818E-4	0.0	0.0	26.488535547416593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTCG	20	7.0308044E-4	45.0	28
TATGGAT	20	7.0308044E-4	45.0	36
TCCCTAT	35	1.2107375E-7	45.0	15
ATTCGAA	20	7.0308044E-4	45.0	34
CTAAGAT	20	7.0308044E-4	45.0	12
ACTTACG	25	3.888748E-5	45.0	26
GTTACTA	20	7.0308044E-4	45.0	31
TCTACGG	20	7.0308044E-4	45.0	2
TACGAGA	40	6.8066583E-9	45.0	24
TCTAACG	40	6.8066583E-9	45.0	24
TCGCTAG	25	3.888748E-5	45.0	15
GTATCGC	20	7.0308044E-4	45.0	1
CGTACGA	20	7.0308044E-4	45.0	2
CGTGTCA	20	7.0308044E-4	45.0	19
ACCGTTG	20	7.0308044E-4	45.0	27
CAGTACG	20	7.0308044E-4	45.0	1
TCAACGG	25	3.888748E-5	45.0	2
GACTAGT	20	7.0308044E-4	45.0	11
TTAGCGG	70	0.0	41.785713	2
TAAGACG	55	6.002665E-11	40.909092	1
>>END_MODULE