FastQCFastQC Report
Sat 18 Jun 2016
SRR3553978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563687
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.20739444658681694No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC22920.1465766486515524No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG17790.11376957153189865No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC17340.11089175774947288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15830.10123509372399976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG453.8562575E-1045.01
TCGTTTA1100.042.95454438
CATACGA3700.038.9189218
CGTTTTT25200.038.1251
ATAACGC1300.038.0769211
ACGACCA8100.038.05555728
TATGCGG2900.038.017242
ACAACGA4500.038.013
TCAAGCG8150.037.82208617
CACGACC7450.037.7516827
GTCGAAA301.14011025E-437.49999610
ACACGTG7950.037.3584942
AACACGT7950.037.3584941
GTATGCG1150.037.1739121
ACATACG3900.036.92307717
TAATACG552.750312E-936.8181826
ATGGTCA8800.036.8181813
CATGGTC8800.036.562512
GCTACGA2400.036.562510
ACACGAC7700.036.52597426
Produced by FastQC (version 0.11.5)