##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1563687
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824349118461686	31.0	31.0	34.0	30.0	34.0
2	31.944990269791845	33.0	31.0	34.0	30.0	34.0
3	31.962122854509886	33.0	31.0	34.0	30.0	34.0
4	35.59800522738886	37.0	35.0	37.0	33.0	37.0
5	35.430735818613314	37.0	35.0	37.0	33.0	37.0
6	35.36707090357597	37.0	35.0	37.0	33.0	37.0
7	35.69729747705263	37.0	35.0	37.0	35.0	37.0
8	35.56041458424864	37.0	35.0	37.0	35.0	37.0
9	37.31391000884448	39.0	37.0	39.0	34.0	39.0
10	36.718080408675135	39.0	37.0	39.0	32.0	39.0
11	36.6283648837651	39.0	35.0	39.0	32.0	39.0
12	36.52949599248443	39.0	35.0	39.0	32.0	39.0
13	36.42681879429835	39.0	35.0	39.0	32.0	39.0
14	37.449367424554914	39.0	36.0	41.0	32.0	41.0
15	37.593047073998825	39.0	36.0	41.0	32.0	41.0
16	37.63953208026926	40.0	36.0	41.0	33.0	41.0
17	37.58240427911724	39.0	36.0	41.0	32.0	41.0
18	37.56746522801558	39.0	36.0	41.0	32.0	41.0
19	37.511374079339404	39.0	36.0	41.0	32.0	41.0
20	37.407004087135086	39.0	36.0	41.0	32.0	41.0
21	37.312582377419524	39.0	35.0	41.0	32.0	41.0
22	37.31816149907238	39.0	35.0	41.0	32.0	41.0
23	36.82334636023705	39.0	35.0	41.0	31.0	41.0
24	36.90378701108342	39.0	35.0	41.0	31.0	41.0
25	36.89240813538771	39.0	35.0	41.0	31.0	41.0
26	36.923893336709966	39.0	35.0	41.0	31.0	41.0
27	34.52646917189949	39.0	35.0	40.0	23.0	41.0
28	35.35885570449841	38.0	34.0	40.0	22.0	41.0
29	36.022987976494015	38.0	34.0	40.0	27.0	41.0
30	36.29359008548386	38.0	35.0	40.0	30.0	41.0
31	36.371711857935765	38.0	35.0	40.0	30.0	41.0
32	36.13309313180962	38.0	35.0	40.0	30.0	41.0
33	36.14043731258238	38.0	35.0	40.0	30.0	41.0
34	36.081647414092465	38.0	35.0	40.0	29.0	41.0
35	35.993569684981715	38.0	35.0	40.0	29.0	41.0
36	35.87035640764424	38.0	35.0	40.0	29.0	41.0
37	35.78954675711955	38.0	35.0	40.0	28.0	41.0
38	35.660065601363954	38.0	34.0	40.0	28.0	41.0
39	35.532639204649016	38.0	34.0	40.0	27.0	41.0
40	35.43592995273351	38.0	34.0	40.0	27.0	41.0
41	35.29451290443676	38.0	34.0	40.0	26.0	41.0
42	35.22364897834413	38.0	34.0	40.0	26.0	41.0
43	35.12789643963274	38.0	34.0	40.0	26.0	41.0
44	34.9966662126116	37.0	34.0	40.0	26.0	41.0
45	34.91044563266178	37.0	34.0	40.0	26.0	41.0
46	34.741299249785925	37.0	33.0	40.0	25.0	41.0
47	34.61501950198473	37.0	33.0	40.0	24.0	41.0
48	34.5052526496671	37.0	33.0	40.0	24.0	41.0
49	34.44722824964331	36.0	33.0	40.0	24.0	41.0
50	34.33461300119525	36.0	33.0	40.0	24.0	41.0
51	32.779159767907515	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	9.0
12	14.0
13	27.0
14	56.0
15	113.0
16	224.0
17	499.0
18	1007.0
19	1764.0
20	2761.0
21	4133.0
22	6059.0
23	8364.0
24	10990.0
25	14156.0
26	18315.0
27	22683.0
28	26774.0
29	32016.0
30	39234.0
31	48529.0
32	61015.0
33	80489.0
34	117354.0
35	134967.0
36	149478.0
37	198226.0
38	273076.0
39	311300.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16236305603359	18.286524093376745	21.314367900992973	15.23674494959669
2	29.64301679300269	25.757200769719258	25.679819554680698	18.91996288259735
3	33.226278660627095	25.14454619114951	25.219817009414285	16.40935813880911
4	29.041106052553996	26.030912836136643	25.230560847535344	19.697420263774017
5	25.188544766311928	30.834815407431282	24.354234575078003	19.622405251178783
6	24.870322513393024	36.0197405235191	24.341124534513618	14.76881242857426
7	82.45422517421966	5.671211693900378	7.7504001759943	4.124162955885673
8	83.88660902085904	5.288014800916041	6.715282534164446	4.11009364406048
9	77.96982388419165	6.955931717792627	8.972703616516604	6.101540781499111
10	40.46001533554989	26.851921132554025	15.9914356261835	16.69662790571259
11	31.642521809032115	26.979056550319854	22.181677023598713	19.19674461704932
12	26.982957586780476	23.831367786519937	27.930973398128913	21.25470122857068
13	25.533306857446536	24.338054866479034	29.806028955922763	20.322609320151667
14	22.373339421508266	26.354762813785626	29.183589810492766	22.08830795421334
15	21.85795494878451	24.61765046329604	31.317264900200612	22.207129687718837
16	24.628969864173584	24.70392092535143	28.7022914432364	21.964817767238586
17	24.788976310476457	23.88988333342926	27.32477791271527	23.996362443379013
18	26.180495201405396	23.357359880845717	27.488941201148307	22.973203716600572
19	25.55140510856712	25.669779182150904	25.407322565193674	23.371493144088298
20	26.330844983682795	25.933322973203715	25.848651296583014	21.887180746530476
21	25.66037832379498	26.785731415558228	26.420760676529255	21.133129584117537
22	24.82299846452647	23.676925113529755	26.6034698760046	24.896606545939182
23	23.455589257952518	26.854926849171225	26.666973633470125	23.022510259406133
24	23.165249823014452	25.950398001646107	27.635070189878153	23.249281985461284
25	24.321619352210515	26.717942913127757	25.692865643827695	23.267572090834037
26	23.146256252050442	25.858819571947585	26.055214374743795	24.939709801258182
27	22.697573107661572	29.717264388589275	24.894432197748014	22.690730306001136
28	23.046811798013287	26.569064013450262	26.461369826570152	23.9227543619663
29	23.985426751005797	26.107974294088265	25.739294372850836	24.167304582055106
30	25.448315423738894	25.2718734631675	26.17090248879731	23.108908624296294
31	26.829602087885874	25.486366517084303	24.041128435550082	23.64290295947974
32	25.246612653299543	26.99344561923198	24.337095595218226	23.422846132250253
33	25.010248214636306	26.495455932037547	23.61425272449026	24.880043128835887
34	23.999112354326666	26.68193826513874	25.417682694810406	23.90126668572419
35	24.014332791664827	27.128319158501668	24.55216421189151	24.305183837941993
36	24.967912376325952	28.81331110382065	23.70480793151059	22.51396858834281
37	24.47625387945286	28.558912365454216	24.009280629691236	22.955553125401696
38	23.798113049478573	29.528479804462147	24.062168451870484	22.6112386941888
39	23.97513057280645	27.044478850306998	24.237459286928907	24.742931289957646
40	24.730716569236684	26.362884643793805	25.903457661283873	23.002941125685638
41	22.235332262786606	27.008985813657084	25.416275763627887	25.339406159928423
42	23.50048315295836	26.95903975667765	25.813605919854805	23.726871170509188
43	24.05871507533157	25.0017426761238	26.27073065133879	24.668811597205835
44	24.393884453858092	25.659738809621107	25.864831005181983	24.081545731338817
45	24.140381035335075	24.682241394857154	25.54168449312426	25.63569307668351
46	23.92000445101865	26.063975718925846	25.28255334987117	24.733466480184333
47	22.963611003992487	26.605132612856664	26.96415587006863	23.467100513082222
48	22.531619179541686	27.298174123082177	26.111235816375018	24.05897088100112
49	23.677948336207947	26.36512294340236	26.25519045691369	23.701738263476003
50	22.302545202460593	25.797618065507994	27.257692875876057	24.642143856155354
51	22.67768421685414	25.27443151986299	25.842959620435547	26.20492464284732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3176.0
1	3142.5
2	3109.0
3	5056.0
4	7003.0
5	5124.5
6	3246.0
7	3211.5
8	3177.0
9	3195.5
10	3214.0
11	3282.5
12	3351.0
13	3463.5
14	3576.0
15	3526.0
16	3476.0
17	3477.0
18	3478.0
19	3714.0
20	3950.0
21	4272.5
22	4595.0
23	4795.0
24	4995.0
25	7100.5
26	10668.0
27	12130.0
28	15634.5
29	19139.0
30	20968.5
31	22798.0
32	25685.5
33	28573.0
34	32966.5
35	37360.0
36	38901.0
37	40442.0
38	44538.5
39	48635.0
40	54411.5
41	60188.0
42	65026.5
43	69865.0
44	76521.5
45	83178.0
46	97460.5
47	111743.0
48	119194.5
49	126646.0
50	128266.5
51	129887.0
52	121316.0
53	112745.0
54	105214.5
55	97684.0
56	94119.0
57	90554.0
58	85422.5
59	80291.0
60	76390.5
61	72490.0
62	67291.5
63	62093.0
64	56029.5
65	49966.0
66	43731.0
67	37496.0
68	34355.0
69	31214.0
70	27712.0
71	24210.0
72	22048.0
73	19886.0
74	16956.5
75	11719.5
76	9412.0
77	7336.5
78	5261.0
79	4263.0
80	3265.0
81	2392.0
82	1519.0
83	1122.0
84	725.0
85	539.5
86	354.0
87	245.5
88	137.0
89	128.0
90	119.0
91	82.5
92	46.0
93	37.5
94	29.0
95	20.0
96	11.0
97	13.0
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1563687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.191520685094744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31011167047909	20.477964592846266
2	9.036657839447914	4.9144093713094295
3	3.3942879624926654	2.768875540298622
4	1.8267716050478413	1.986907915424084
5	1.131379785935207	1.5381968425977623
6	0.8098372085911872	1.3212423125379993
7	0.6082043871127022	1.1576601521058287
8	0.4925112516767187	1.071370391008752
9	0.4189235519646669	1.0252051585848543
>10	5.750574530005262	38.470905262096686
>50	1.1337434051822073	19.890685375455305
>100	0.08166552185505865	3.796056509537941
>500	0.0036349637791897042	0.6959667441752271
>1k	0.0016963164302885286	0.8845538320212317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3243	0.20739444658681694	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	2292	0.1465766486515524	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	1779	0.11376957153189865	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1734	0.11089175774947288	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1583	0.10123509372399976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5580566954895703E-4	0.0	0.0	0.06037013801355386	0.0
2	2.5580566954895703E-4	0.0	0.0	0.32602432584014573	0.0
3	2.5580566954895703E-4	0.0	0.0	0.4772694279609666	0.0
4	2.5580566954895703E-4	0.0	0.0	0.7002680203902699	0.0
5	2.5580566954895703E-4	0.0	0.0	1.403030146058642	0.0
6	2.5580566954895703E-4	0.0	0.0	2.0762467168941097	0.0
7	2.5580566954895703E-4	0.0	0.0	2.607043481208196	0.0
8	3.197570869361963E-4	0.0	0.0	3.464631988371074	0.0
9	3.197570869361963E-4	0.0	0.0	3.905129351334378	0.0
10	3.197570869361963E-4	0.0	0.0	4.825326296119364	0.0
11	3.197570869361963E-4	0.0	0.0	5.67556039028271	0.0
12	3.197570869361963E-4	0.0	0.0	6.473674079275456	0.0
13	3.197570869361963E-4	0.0	0.0	6.791192866603099	0.0
14	3.197570869361963E-4	0.0	0.0	6.933484770289707	0.0
15	3.197570869361963E-4	0.0	0.0	7.159425127918823	0.0
16	3.197570869361963E-4	0.0	0.0	7.563214377301851	0.0
17	3.197570869361963E-4	0.0	0.0	7.990985408205095	0.0
18	3.8370850432343557E-4	0.0	0.0	8.53847349245725	0.0
19	3.8370850432343557E-4	0.0	0.0	8.825103745186857	0.0
20	3.8370850432343557E-4	0.0	0.0	9.094275260969747	0.0
21	3.8370850432343557E-4	0.0	0.0	9.410770825619194	0.0
22	3.8370850432343557E-4	0.0	0.0	9.742422876189416	0.0
23	3.8370850432343557E-4	0.0	0.0	10.08034216566359	0.0
24	3.8370850432343557E-4	0.0	0.0	10.358658734132854	0.0
25	3.8370850432343557E-4	0.0	0.0	10.613633035255777	0.0
26	3.8370850432343557E-4	0.0	0.0	10.852491579197116	0.0
27	3.8370850432343557E-4	0.0	0.0	11.111239013945886	0.0
28	3.8370850432343557E-4	0.0	0.0	11.390578805093346	0.0
29	3.8370850432343557E-4	0.0	0.0	11.678232280501149	0.0
30	3.8370850432343557E-4	0.0	0.0	12.050109772607945	0.0
31	3.8370850432343557E-4	0.0	0.0	12.34096081888511	0.0
32	3.8370850432343557E-4	0.0	0.0	12.649334553526376	0.0
33	3.8370850432343557E-4	0.0	0.0	12.954510717298282	0.0
34	3.8370850432343557E-4	0.0	0.0	13.261349617922257	0.0
35	3.8370850432343557E-4	0.0	0.0	13.589484340536181	0.0
36	4.4765992171067484E-4	0.0	0.0	13.873876293657235	0.0
37	4.4765992171067484E-4	0.0	0.0	14.179500117350852	0.0
38	4.4765992171067484E-4	0.0	0.0	14.507442985712613	0.0
39	4.4765992171067484E-4	0.0	0.0	14.945062534893491	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	45	3.8562575E-10	45.0	1
TCGTTTA	110	0.0	42.954544	38
CATACGA	370	0.0	38.91892	18
CGTTTTT	2520	0.0	38.125	1
ATAACGC	130	0.0	38.07692	11
ACGACCA	810	0.0	38.055557	28
TATGCGG	290	0.0	38.01724	2
ACAACGA	450	0.0	38.0	13
TCAAGCG	815	0.0	37.822086	17
CACGACC	745	0.0	37.75168	27
GTCGAAA	30	1.14011025E-4	37.499996	10
ACACGTG	795	0.0	37.35849	42
AACACGT	795	0.0	37.35849	41
GTATGCG	115	0.0	37.173912	1
ACATACG	390	0.0	36.923077	17
TAATACG	55	2.750312E-9	36.81818	26
ATGGTCA	880	0.0	36.81818	13
CATGGTC	880	0.0	36.5625	12
GCTACGA	240	0.0	36.5625	10
ACACGAC	770	0.0	36.525974	26
>>END_MODULE