SRR3553977_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768077
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1339	0.17433147978653182	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1302	0.1695142544302199	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1203	0.15662492172008796	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	1118	0.14555832292856055	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	970	0.12628942150331282	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	957	0.12459688286460863	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	845	0.1100150115157725	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	816	0.10623934839866316	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	808	0.10519778615946057	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	797	0.10376563808055703	Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	781	0.10168251360215187	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	7.031824E-4	45.0	1
TAGCCGT	25	3.889593E-5	45.0	44
CCCGTTA	25	3.889593E-5	45.0	36
CGTCGTT	20	7.031824E-4	45.0	13
CGACTTA	20	7.031824E-4	45.0	26
AAAATCG	20	7.031824E-4	45.0	35
GCCGTAT	20	7.031824E-4	45.0	43
GTACGAT	25	3.889593E-5	45.0	10
GTACGAG	100	0.0	42.75	1
TCTAACG	70	0.0	41.785713	24
TTAACGG	60	3.6379788E-12	41.250004	2
ACGTAAG	55	6.002665E-11	40.909092	1
TCGTGTA	165	0.0	40.90909	17
CGTGTAC	165	0.0	40.90909	18
GCGTAAG	145	0.0	40.344826	1
TTGGACG	45	1.9268555E-8	40.0	1
GCGATAT	45	1.9268555E-8	40.0	9
AACACGT	225	0.0	40.0	41
CCCTCGT	170	0.0	39.705883	14
CGTAAGG	245	0.0	39.489796	2