##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768077
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85440392044027	31.0	31.0	34.0	30.0	34.0
2	31.96235663872242	33.0	31.0	34.0	30.0	34.0
3	31.98094592078659	33.0	31.0	34.0	30.0	34.0
4	35.633623972596496	37.0	35.0	37.0	33.0	37.0
5	35.45687997427341	37.0	35.0	37.0	33.0	37.0
6	35.41250942288338	37.0	35.0	37.0	33.0	37.0
7	35.683444498403155	37.0	35.0	37.0	35.0	37.0
8	35.52608397335163	37.0	35.0	37.0	33.0	37.0
9	37.29679576396637	39.0	37.0	39.0	34.0	39.0
10	36.746626965785985	39.0	37.0	39.0	32.0	39.0
11	36.665211951405915	39.0	35.0	39.0	32.0	39.0
12	36.572640503491186	39.0	35.0	39.0	32.0	39.0
13	36.474009767249896	39.0	35.0	39.0	32.0	39.0
14	37.571592431487986	39.0	36.0	41.0	32.0	41.0
15	37.69365571420574	40.0	36.0	41.0	33.0	41.0
16	37.722418455441314	40.0	36.0	41.0	33.0	41.0
17	37.639555669548756	39.0	36.0	41.0	33.0	41.0
18	37.632389721343046	39.0	36.0	41.0	33.0	41.0
19	37.56819824054099	39.0	36.0	41.0	32.0	41.0
20	37.474570908906266	39.0	36.0	41.0	32.0	41.0
21	37.388276175435536	39.0	35.0	41.0	32.0	41.0
22	37.37123621720218	39.0	35.0	41.0	32.0	41.0
23	36.87195033831244	39.0	35.0	41.0	31.0	41.0
24	36.96674161574946	39.0	35.0	41.0	31.0	41.0
25	36.970413122642654	39.0	35.0	41.0	31.0	41.0
26	36.96860080434644	39.0	35.0	41.0	31.0	41.0
27	34.57234626215861	39.0	35.0	40.0	23.0	41.0
28	35.41297031417423	38.0	35.0	40.0	23.0	41.0
29	36.09792247391863	38.0	35.0	40.0	28.0	41.0
30	36.37611333238725	38.0	35.0	40.0	30.0	41.0
31	36.47192924667709	38.0	35.0	40.0	30.0	41.0
32	36.25506557285272	38.0	35.0	40.0	30.0	41.0
33	36.2742967176468	38.0	35.0	40.0	30.0	41.0
34	36.215535681969385	38.0	35.0	40.0	30.0	41.0
35	36.12666828976782	38.0	35.0	40.0	30.0	41.0
36	35.97876775375386	38.0	35.0	40.0	29.0	41.0
37	35.88666500884677	38.0	35.0	40.0	29.0	41.0
38	35.79416516833599	38.0	35.0	40.0	29.0	41.0
39	35.70643307897515	38.0	35.0	40.0	28.0	41.0
40	35.582531438905214	38.0	34.0	40.0	28.0	41.0
41	35.42509149473295	38.0	34.0	40.0	27.0	41.0
42	35.31579516116223	38.0	34.0	40.0	27.0	41.0
43	35.201612598736844	38.0	34.0	40.0	26.0	41.0
44	35.07935011724085	37.0	34.0	40.0	26.0	41.0
45	35.001990685829675	37.0	34.0	40.0	26.0	41.0
46	34.86714873638971	37.0	34.0	40.0	26.0	41.0
47	34.74975165250359	37.0	33.0	40.0	26.0	41.0
48	34.63835136320968	37.0	33.0	40.0	25.0	41.0
49	34.579456226393965	37.0	33.0	40.0	25.0	41.0
50	34.463159292623004	36.0	33.0	40.0	24.0	41.0
51	32.87417407369313	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	9.0
12	12.0
13	20.0
14	30.0
15	64.0
16	109.0
17	200.0
18	387.0
19	782.0
20	1276.0
21	2161.0
22	3059.0
23	3851.0
24	5135.0
25	6552.0
26	8218.0
27	10073.0
28	12185.0
29	14785.0
30	18571.0
31	23053.0
32	29493.0
33	39090.0
34	58416.0
35	69001.0
36	73087.0
37	98377.0
38	135153.0
39	154893.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35730141639445	17.572977709266127	23.16434419986538	13.905376674474043
2	30.12263093413811	25.775540733546247	26.56074846662509	17.54107986569055
3	33.29861459202658	25.243562819873528	25.726847698863526	15.730974889236366
4	29.098254471882377	26.783772981094344	25.24408360099313	18.873888946030153
5	25.04384326050643	31.750722909291646	24.124273998570455	19.081159831631464
6	24.48947175869086	37.47202428923142	23.47238623210954	14.56611771996818
7	81.70912551736349	6.386469064950519	7.641681758469529	4.262723659216459
8	83.4452795748343	5.998617326127459	6.38672945551032	4.169373643527927
9	77.31646696880651	7.7011810013839765	8.902232458464452	6.080119571345061
10	41.66991069905752	26.493177116356826	15.500789634372595	16.336122550213066
11	31.540848118092324	26.29488970506863	21.93530075760633	20.228961419232707
12	27.269791960962248	23.525896492148572	27.834188499330146	21.370123047559034
13	25.788299870976477	23.734599525828788	29.644163280504426	20.832937322690302
14	22.445796450095497	27.74969176267484	27.835490452129147	21.969021335100518
15	21.91017306858557	25.24890082634944	30.697443094898034	22.14348301016695
16	23.756732723411844	24.168149807896864	29.43832454298202	22.63679292570927
17	24.280898920290543	24.02832007728392	27.217583653722215	24.47319734870332
18	24.653648006645167	24.569411660549658	27.46117902241572	23.31576131038945
19	25.569441605464032	26.405685888263807	25.181069085521372	22.84380342075078
20	26.120558225282103	25.36581618769993	25.827488650226478	22.686136936791492
21	25.718254810390107	27.022811514991336	25.92396335263261	21.334970321985946
22	24.167238440937563	24.49012273509036	25.48585623576803	25.856782588204048
23	23.83068364239523	26.81541043411012	25.56892082434443	23.784985099150216
24	23.538134848459205	25.608630384714033	27.059786974483025	23.793447792343734
25	24.478795745739035	26.434198654561982	25.241870281234824	23.845135318464163
26	22.630934138113755	26.559316318546188	26.096341903220644	24.713407640119414
27	23.25756402027401	30.43340706726018	24.185986561243208	22.1230423512226
28	23.184394272970028	26.844443981527892	27.171624719917403	22.799537025584673
29	24.31592145058373	25.34928138715259	26.12784916095652	24.20694800130716
30	24.676822766467424	26.26598635293076	25.843763060214016	23.2134278203878
31	26.230182650958174	26.891965258691513	23.142731783401924	23.735120306948392
32	26.051554726934928	26.69419862852292	24.296522353878583	22.957724290663567
33	25.290563315917545	26.801479539160788	23.0909140620016	24.81704308292007
34	24.322691605138548	26.66151961326794	25.00660741045494	24.00918137113857
35	25.729451604461534	25.5952202708843	24.77590137447157	23.8994267501826
36	25.61487975814925	28.29612135241649	23.545425784133624	22.54357310530064
37	25.398104617115212	28.4619901390095	24.468900904466608	21.67100433940868
38	25.21127439045825	27.403632708699778	24.19887589395334	23.186217006888633
39	25.508900800310386	26.03475953582779	24.226216902732407	24.23012276112942
40	24.701690064928385	25.654328927959046	26.694068433243025	22.949912573869547
41	23.051725282751597	27.113818015641662	25.834649390620996	23.999807310985748
42	24.101359629308	26.813587700191515	25.91406851136019	23.170984159140296
43	24.337013085927584	25.304233820307076	26.313507630094378	24.045245463670962
44	24.613547860435865	25.105425627899287	25.399797155753916	24.881229355910932
45	24.618365085792178	24.67565100894832	25.589621873848582	25.116362031410915
46	25.232105635242302	25.942581277658363	25.38508508912518	23.440227997974162
47	24.31123442050732	25.581419571214862	26.872435966706465	23.234910041571354
48	23.609612057124483	26.42430381328955	25.983462595547064	23.982621534038906
49	24.351464761996517	26.121599787521305	26.517263243138384	23.009672207343794
50	23.134008699648604	24.651434686886862	27.569631690572688	24.644924922891846
51	23.98626700187611	24.482571408856142	25.499787130717362	26.031374458550378
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2026.0
1	2096.0
2	2166.0
3	2624.0
4	3082.0
5	2379.5
6	1677.0
7	1674.0
8	1671.0
9	1706.0
10	1741.0
11	1756.5
12	1772.0
13	1865.5
14	1959.0
15	2024.0
16	2089.0
17	1913.5
18	1738.0
19	1724.0
20	1710.0
21	1833.5
22	1957.0
23	2467.0
24	2977.0
25	3945.0
26	5801.0
27	6689.0
28	7768.0
29	8847.0
30	10489.5
31	12132.0
32	12738.5
33	13345.0
34	15208.5
35	17072.0
36	18193.0
37	19314.0
38	21460.0
39	23606.0
40	26312.0
41	29018.0
42	31424.5
43	33831.0
44	37359.0
45	40887.0
46	48800.0
47	56713.0
48	60161.0
49	63609.0
50	62907.5
51	62206.0
52	57180.5
53	52155.0
54	49006.0
55	45857.0
56	42433.0
57	39009.0
58	37778.5
59	36548.0
60	36621.5
61	36695.0
62	33597.5
63	30500.0
64	28438.0
65	26376.0
66	23652.5
67	20929.0
68	19074.5
69	17220.0
70	15543.5
71	13867.0
72	12288.0
73	10709.0
74	9236.5
75	6496.0
76	5228.0
77	4228.0
78	3228.0
79	2447.0
80	1666.0
81	1242.5
82	819.0
83	630.0
84	441.0
85	320.0
86	199.0
87	119.5
88	40.0
89	34.0
90	28.0
91	22.5
92	17.0
93	13.5
94	10.0
95	16.5
96	23.0
97	11.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	768077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.202930381441885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63038021648676	22.842591084005292
2	8.894899424598611	5.373040561421586
3	3.3321496644358977	3.0192205300650703
4	1.7866492999496144	2.158481776897203
5	1.0931162365605727	1.650765679583137
6	0.778415554651016	1.4106258482973664
7	0.6082262138140859	1.2859149794396378
8	0.5408493594779268	1.3068188440927406
9	0.4550350454280147	1.2369052618360716
>10	6.284505738471187	45.43031388075147
>50	0.5473747006342772	10.264009358814397
>100	0.041117348382579556	2.137300395111006
>500	0.0055679742601409815	1.2421231324207649
>1k	0.0017132228492741483	0.6418886672643028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1339	0.17433147978653182	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1302	0.1695142544302199	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1203	0.15662492172008796	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	1118	0.14555832292856055	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	970	0.12628942150331282	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	957	0.12459688286460863	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	845	0.1100150115157725	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	816	0.10623934839866316	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	808	0.10519778615946057	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	797	0.10376563808055703	Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	781	0.10168251360215187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11444165103238348	0.0
2	0.0	0.0	0.0	0.47651472443518034	0.0
3	0.0	0.0	0.0	0.6246769529617473	0.0
4	0.0	0.0	0.0	0.8432748279143888	0.0
5	0.0	0.0	0.0	1.5670303888802815	0.0
6	0.0	0.0	0.0	2.1991284727963474	0.0
7	0.0	0.0	0.0	2.7563642707697276	0.0
8	0.0	0.0	0.0	3.7996190486110115	0.0
9	0.0	0.0	0.0	4.232518354279584	0.0
10	0.0	0.0	0.0	5.222913848481337	0.0
11	0.0	0.0	0.0	6.181671889667312	0.0
12	0.0	0.0	0.0	7.068562136348309	0.0
13	0.0	0.0	0.0	7.391706821060909	0.0
14	0.0	0.0	0.0	7.526979716877344	0.0
15	0.0	0.0	0.0	7.747790911588291	0.0
16	0.0	0.0	0.0	8.161291120551715	1.301952799003225E-4
17	1.301952799003225E-4	0.0	0.0	8.625307098116465	1.301952799003225E-4
18	2.60390559800645E-4	0.0	0.0	9.19230754208237	1.301952799003225E-4
19	2.60390559800645E-4	0.0	0.0	9.500349574326533	1.301952799003225E-4
20	2.60390559800645E-4	0.0	0.0	9.768031069801596	1.301952799003225E-4
21	2.60390559800645E-4	0.0	0.0	10.08453579523928	1.301952799003225E-4
22	2.60390559800645E-4	0.0	0.0	10.411065557229287	1.301952799003225E-4
23	2.60390559800645E-4	0.0	0.0	10.729913797705178	1.301952799003225E-4
24	2.60390559800645E-4	0.0	0.0	11.022853177480904	1.301952799003225E-4
25	2.60390559800645E-4	0.0	0.0	11.262152101937696	1.301952799003225E-4
26	2.60390559800645E-4	0.0	0.0	11.48999384176326	1.301952799003225E-4
27	2.60390559800645E-4	0.0	0.0	11.71978851078733	1.301952799003225E-4
28	2.60390559800645E-4	0.0	0.0	11.97041442459545	1.301952799003225E-4
29	2.60390559800645E-4	0.0	0.0	12.235492014472507	1.301952799003225E-4
30	2.60390559800645E-4	0.0	0.0	12.530254128166838	1.301952799003225E-4
31	2.60390559800645E-4	0.0	0.0	12.791946640766486	1.301952799003225E-4
32	2.60390559800645E-4	0.0	0.0	13.061060284320453	1.301952799003225E-4
33	2.60390559800645E-4	0.0	0.0	13.324575530838706	1.301952799003225E-4
34	2.60390559800645E-4	0.0	0.0	13.581450818082041	1.301952799003225E-4
35	2.60390559800645E-4	0.0	0.0	13.85954793594913	1.301952799003225E-4
36	2.60390559800645E-4	0.0	0.0	14.11759498071157	1.301952799003225E-4
37	2.60390559800645E-4	0.0	0.0	14.369522847318693	1.301952799003225E-4
38	2.60390559800645E-4	0.0	0.0	14.625226377042926	1.301952799003225E-4
39	2.60390559800645E-4	0.0	0.0	14.890043576360183	1.301952799003225E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	7.031824E-4	45.0	1
TAGCCGT	25	3.889593E-5	45.0	44
CCCGTTA	25	3.889593E-5	45.0	36
CGTCGTT	20	7.031824E-4	45.0	13
CGACTTA	20	7.031824E-4	45.0	26
AAAATCG	20	7.031824E-4	45.0	35
GCCGTAT	20	7.031824E-4	45.0	43
GTACGAT	25	3.889593E-5	45.0	10
GTACGAG	100	0.0	42.75	1
TCTAACG	70	0.0	41.785713	24
TTAACGG	60	3.6379788E-12	41.250004	2
ACGTAAG	55	6.002665E-11	40.909092	1
TCGTGTA	165	0.0	40.90909	17
CGTGTAC	165	0.0	40.90909	18
GCGTAAG	145	0.0	40.344826	1
TTGGACG	45	1.9268555E-8	40.0	1
GCGATAT	45	1.9268555E-8	40.0	9
AACACGT	225	0.0	40.0	41
CCCTCGT	170	0.0	39.705883	14
CGTAAGG	245	0.0	39.489796	2
>>END_MODULE