SRR3553976_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600983
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2692	0.4479328034237241	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2304	0.383371909022385	TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1469	0.24443287081331752	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	1424	0.23694513821522406	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	1395	0.23211971054089717	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	1359	0.2261295244624224	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	1226	0.20399911478361285	No Hit
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1176	0.19567941189684232	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.15291613905884194	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	847	0.1409357669018924	No Hit
TCTGTGAGGGATGTGAGGGTATAACAGATAACCAACCATTCCCTTAAAAAT	819	0.13627673328530093	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	805	0.13394721647700517	No Hit
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	801	0.13328164024606354	TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	708	0.11780699287667039	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	616	0.10249873956501265	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	609	0.10133398116086477	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	30	2.163766E-6	45.000004	2
TGTCACG	30	2.163766E-6	45.000004	1
CGAACAG	25	3.888576E-5	45.000004	41
CGCATGT	25	3.888576E-5	45.000004	20
ACCTCGA	25	3.888576E-5	45.000004	31
CGGGATA	25	3.888576E-5	45.000004	6
TTGCGAG	25	3.888576E-5	45.000004	1
CTCGAAC	25	3.888576E-5	45.000004	39
ATACGAG	30	2.163766E-6	45.000004	1
TCGAACA	25	3.888576E-5	45.000004	40
GTTGCAC	45	3.8380676E-10	45.000004	9
ACGAAAT	25	3.888576E-5	45.000004	24
ACACGAA	20	7.030597E-4	45.0	35
GATCGTG	20	7.030597E-4	45.0	29
ACGTTGT	20	7.030597E-4	45.0	29
ACCATAT	20	7.030597E-4	45.0	38
TCCGAGT	20	7.030597E-4	45.0	16
CTAACGG	35	1.2106284E-7	45.0	2
TCAATCG	20	7.030597E-4	45.0	29
TACGCAT	105	0.0	45.0	16