FastQCFastQC Report
Sat 18 Jun 2016
SRR3553972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546015
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.3239295867116425No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC30940.20012742437815934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29070.188031810816842No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT18010.11649304825632352No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC16780.10853710992454796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG453.8562575E-1045.01
CGTTTTT44200.040.9785041
GCGTACG1050.040.7142871
CATACCG403.459245E-739.3750041
AACACGT6000.039.37541
GCGATCA1550.039.1935469
ACGTTAG750.039.01
GTAACGG1500.039.02
TAACGGG4800.038.9062463
CGGGATA5300.038.2075466
ACACGAC6350.037.55905526
TCAAGCG6900.037.517
TCCGATT301.1401043E-437.49999630
CACGACC6400.037.2656327
AAGGGCG6950.036.9064755
ACGACCA6950.036.90647528
ACGGGTA2500.036.8999985
CGACAGG1350.036.6666682
TGGGCGA8450.036.213026
TAGGGCG5600.036.1607175
Produced by FastQC (version 0.11.5)