##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1546015
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720921853927678	31.0	31.0	34.0	30.0	34.0
2	31.835746742431347	31.0	31.0	34.0	30.0	34.0
3	31.841101153611056	33.0	31.0	34.0	30.0	34.0
4	35.50001131942446	37.0	35.0	37.0	33.0	37.0
5	35.35703857983267	37.0	35.0	37.0	33.0	37.0
6	35.31069103469242	37.0	35.0	37.0	32.0	37.0
7	35.72622645963978	37.0	35.0	37.0	35.0	37.0
8	35.634814021856194	37.0	35.0	37.0	35.0	37.0
9	37.378553248189704	39.0	37.0	39.0	35.0	39.0
10	36.681345911909006	39.0	37.0	39.0	32.0	39.0
11	36.587954838730546	39.0	35.0	39.0	32.0	39.0
12	36.59618567737053	39.0	35.0	39.0	32.0	39.0
13	36.572082418346525	39.0	35.0	39.0	32.0	39.0
14	37.65330025905311	39.0	36.0	41.0	32.0	41.0
15	37.76157087738476	40.0	37.0	41.0	33.0	41.0
16	37.78287144691352	40.0	37.0	41.0	33.0	41.0
17	37.70023900156208	39.0	36.0	41.0	33.0	41.0
18	37.65969023586447	39.0	36.0	41.0	33.0	41.0
19	37.59106282927397	39.0	36.0	41.0	32.0	41.0
20	37.49099588296362	39.0	36.0	41.0	32.0	41.0
21	37.40822825134297	39.0	36.0	41.0	32.0	41.0
22	37.414277351772135	39.0	36.0	41.0	32.0	41.0
23	36.91146204920392	39.0	35.0	41.0	31.0	41.0
24	37.01661626827683	39.0	35.0	41.0	31.0	41.0
25	37.0263309217569	39.0	35.0	41.0	31.0	41.0
26	37.046742107935565	39.0	35.0	41.0	31.0	41.0
27	34.711494390416654	39.0	35.0	40.0	24.0	41.0
28	35.57788895968021	39.0	35.0	40.0	24.0	41.0
29	36.2466418501761	39.0	35.0	40.0	29.0	41.0
30	36.49135357677642	39.0	35.0	40.0	30.0	41.0
31	36.52411393162421	39.0	35.0	40.0	30.0	41.0
32	36.2955753986863	39.0	35.0	40.0	30.0	41.0
33	36.26745794833815	39.0	35.0	40.0	30.0	41.0
34	36.2343179076529	39.0	35.0	40.0	30.0	41.0
35	36.11898138116383	39.0	35.0	40.0	29.0	41.0
36	36.03280045795157	39.0	35.0	40.0	29.0	41.0
37	35.993694757166004	39.0	35.0	40.0	29.0	41.0
38	35.93474384142456	38.0	35.0	40.0	29.0	41.0
39	35.821363311481456	38.0	35.0	40.0	28.0	41.0
40	35.741043909664526	38.0	35.0	40.0	28.0	41.0
41	35.60937442392215	38.0	34.0	40.0	27.0	41.0
42	35.51917348796745	38.0	34.0	40.0	27.0	41.0
43	35.39755888526308	38.0	34.0	40.0	27.0	41.0
44	35.294569586970375	38.0	34.0	40.0	26.0	41.0
45	35.22652302856053	38.0	34.0	40.0	26.0	41.0
46	35.06704268716668	38.0	34.0	40.0	26.0	41.0
47	34.928478054870105	38.0	34.0	40.0	25.0	41.0
48	34.8490874926828	38.0	34.0	40.0	25.0	41.0
49	34.78811525114569	37.0	33.0	40.0	25.0	41.0
50	34.674620880133766	37.0	33.0	40.0	24.0	41.0
51	33.086050264712824	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	7.0
11	5.0
12	13.0
13	23.0
14	48.0
15	98.0
16	200.0
17	432.0
18	818.0
19	1514.0
20	2383.0
21	3775.0
22	5585.0
23	7904.0
24	10868.0
25	14610.0
26	18410.0
27	21339.0
28	24640.0
29	29946.0
30	36009.0
31	45377.0
32	57169.0
33	74488.0
34	105453.0
35	128215.0
36	146888.0
37	198401.0
38	284653.0
39	326686.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.044407719200656	19.01055293771406	23.902420092948645	14.042619250136642
2	31.556679592371356	24.381716865619026	25.87814477867291	18.183458763336706
3	32.565013922892085	24.415028314731746	27.362218348463628	15.657739413912545
4	26.95879406085969	27.164419491402086	27.009311035145195	18.86747541259302
5	24.591223241689118	31.218649236909084	25.4701280388612	18.719999482540597
6	24.003971500923342	37.2539076270282	24.702024236504823	14.04009663554364
7	82.64479969469896	5.268448236271964	8.309815881475924	3.7769361875531606
8	84.01787822239758	4.579386357829645	7.474442356639489	3.9282930631332813
9	77.71328221265641	7.399087330976736	9.755856185095228	5.131774271271624
10	35.43710766066306	33.136289104568846	16.661157880098187	14.76544535466991
11	27.297406558151117	27.285375627015263	27.596304046209124	17.820913768624496
12	23.563031406551683	24.547109827524313	31.368000957299895	20.52185780862411
13	23.14505357321889	24.899370316588133	33.14262798226408	18.8129481279289
14	20.997467683043176	28.19228791441222	30.451515670934633	20.35872873160998
15	20.437835337949505	27.260149481085243	32.72794895262982	19.57406622833543
16	23.681594292422776	26.18583907659369	30.08301989307995	20.04954673790358
17	22.98781059692176	25.72834028130387	29.722091959004278	21.56175716277009
18	24.086635640663253	25.122460002005155	30.157275317509857	20.633629039821734
19	23.407211443614713	27.615385361720296	28.32643926481955	20.650963929845442
20	24.291161469972803	27.133565974456907	28.22145968829539	20.353812867274897
21	23.949120804131912	27.99727040164552	28.550175774491194	19.50343301973137
22	23.029854173471797	25.409649971054616	28.046687774698174	23.513808080775416
23	21.216223645954276	28.720419918306096	28.420164099313393	21.64319233642623
24	21.517902478307132	27.07942678434556	30.0305624460306	21.372108291316707
25	22.43820402777463	28.25030805005126	27.58964175638658	21.721846165787525
26	21.994417906682663	28.45179380536411	27.750830360636865	21.802957927316356
27	21.412276077528354	31.781580385701304	26.12872449491111	20.67741904185923
28	21.22786648253736	28.116803523898536	28.617510179396703	22.037819814167392
29	23.03477003780688	26.34553998505836	27.752447421273402	22.86724255586136
30	24.102159422774037	26.616624030167884	27.57819296707988	21.7030235799782
31	23.92538235398751	27.621077415160915	25.831508749915105	22.62203148093647
32	24.227449280893136	27.267393912736942	26.178141867963767	22.327014938406162
33	22.564658169552043	27.37276158381387	26.120768556579332	23.941811690054752
34	23.078236627717068	26.906724708363118	27.140939770959534	22.874098892960287
35	22.288140800703747	26.63066011649305	26.568888400177233	24.512310682625976
36	22.843892200269725	29.2062496159481	26.05802660388159	21.891831579900582
37	22.21828378120523	28.213180337836306	26.359252659256217	23.209283221702247
38	22.175528697975118	27.608011565217673	26.673673929425007	23.542785807382206
39	22.57313156728751	26.49333932723809	26.602393896566333	24.331135208908066
40	22.940204331782034	26.030859985187725	28.085885324527897	22.943050358502344
41	21.08491832226725	27.161056005278084	26.901614796751648	24.85241087570302
42	22.752366568241573	27.596239363783663	26.88880767651025	22.762586391464506
43	22.80876964324408	26.304660692166635	27.855809937161023	23.03075972742826
44	22.93884600084734	26.721797653968427	26.895469966332797	23.443886378851435
45	22.84492711907711	25.055254961950563	27.50788317060313	24.591934748369194
46	23.25384941284528	25.907769329534318	27.007758656934115	23.83062260068628
47	21.91983907012545	26.658538241866992	27.80057114581683	23.62105154219073
48	21.920938671358297	26.91726794371335	27.151677053586155	24.010116331342193
49	22.820671209529014	25.81475600172055	27.956067696626484	23.408505092123942
50	21.80832656862967	25.162886517918647	28.587238804280684	24.441548109171
51	21.889114918031197	24.85325174723402	26.945404798789145	26.312228535945643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3255.0
1	3345.5
2	3436.0
3	6941.0
4	10446.0
5	7561.5
6	4677.0
7	4620.5
8	4564.0
9	4756.0
10	4948.0
11	4966.0
12	4984.0
13	5011.0
14	5038.0
15	4813.5
16	4589.0
17	4679.0
18	4769.0
19	4962.0
20	5155.0
21	5792.0
22	6429.0
23	7285.5
24	8142.0
25	9535.0
26	13408.0
27	15888.0
28	18938.0
29	21988.0
30	25135.5
31	28283.0
32	32308.5
33	36334.0
34	39608.5
35	42883.0
36	46456.0
37	50029.0
38	54368.0
39	58707.0
40	64894.5
41	71082.0
42	75376.5
43	79671.0
44	86566.5
45	93462.0
46	99647.5
47	105833.0
48	119446.0
49	133059.0
50	139301.0
51	145543.0
52	140075.0
53	134607.0
54	119703.0
55	104799.0
56	91872.5
57	78946.0
58	69607.5
59	60269.0
60	54492.0
61	48715.0
62	44265.0
63	39815.0
64	34682.5
65	29550.0
66	25600.5
67	21651.0
68	19846.5
69	18042.0
70	15824.5
71	13607.0
72	12661.5
73	11716.0
74	10012.0
75	6794.5
76	5281.0
77	4205.0
78	3129.0
79	2485.5
80	1842.0
81	1333.5
82	825.0
83	615.5
84	406.0
85	299.5
86	193.0
87	138.5
88	84.0
89	75.5
90	67.0
91	44.0
92	21.0
93	16.5
94	12.0
95	9.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1546015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.719941109366438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00046636367145	23.040099098670055
2	9.492602257668615	5.832243646604375
3	3.5615025158741878	3.2822744264554644
4	1.8991793812970243	2.3337071499827036
5	1.2387321443131358	1.9026889261789373
6	0.8930580258316525	1.646081397647725
7	0.69469975767637	1.4938794951156458
8	0.5605124630847568	1.3775127885623726
9	0.48165121736163813	1.3316667329343759
>10	5.366059801854538	37.59468546506931
>50	0.7392653824436934	14.712884366744216
>100	0.06639325432536264	3.3492184572474826
>500	0.003482924205963466	0.6576862828650463
>1k	0.0021768276287271664	1.1104767069910482
>5k	2.1768276287271662E-4	0.3348950589311796
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5008	0.3239295867116425	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	3094	0.20012742437815934	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2907	0.188031810816842	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	1801	0.11649304825632352	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1678	0.10853710992454796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9404727638476987E-4	0.0	0.0	0.12942953334864152	0.0
2	1.9404727638476987E-4	0.0	0.0	0.5287141457230363	0.0
3	2.5872970184635986E-4	0.0	0.0	0.7609240531301443	0.0
4	2.5872970184635986E-4	0.0	0.0	1.0898341866023291	0.0
5	2.5872970184635986E-4	0.0	0.0	2.0074190742004445	0.0
6	2.5872970184635986E-4	0.0	0.0	2.862650103653587	0.0
7	2.5872970184635986E-4	0.0	0.0	3.5670417169303015	0.0
8	2.5872970184635986E-4	0.0	0.0	4.898141350504361	0.0
9	2.5872970184635986E-4	0.0	0.0	5.4894680840742165	0.0
10	2.5872970184635986E-4	0.0	0.0	6.666106085646	0.0
11	2.5872970184635986E-4	0.0	0.0	7.871980543526421	0.0
12	2.5872970184635986E-4	0.0	0.0	8.920612025109717	0.0
13	2.5872970184635986E-4	0.0	0.0	9.368602503856689	0.0
14	2.5872970184635986E-4	0.0	0.0	9.565754536663615	0.0
15	2.5872970184635986E-4	0.0	0.0	9.844665155253992	0.0
16	2.5872970184635986E-4	0.0	0.0	10.383340394498113	0.0
17	2.5872970184635986E-4	0.0	0.0	11.028806318179319	0.0
18	2.5872970184635986E-4	0.0	0.0	11.776341109238913	0.0
19	2.5872970184635986E-4	0.0	0.0	12.186621733941779	6.468242546158997E-5
20	2.5872970184635986E-4	0.0	0.0	12.593926967073411	6.468242546158997E-5
21	2.5872970184635986E-4	0.0	0.0	13.048967830195696	6.468242546158997E-5
22	2.5872970184635986E-4	0.0	0.0	13.541847912213012	6.468242546158997E-5
23	2.5872970184635986E-4	0.0	0.0	14.04708233749349	6.468242546158997E-5
24	2.5872970184635986E-4	0.0	0.0	14.463184380487899	6.468242546158997E-5
25	2.5872970184635986E-4	0.0	0.0	14.82404763213811	6.468242546158997E-5
26	2.5872970184635986E-4	0.0	0.0	15.169645831379386	6.468242546158997E-5
27	3.234121273079498E-4	0.0	0.0	15.533807886728136	6.468242546158997E-5
28	3.234121273079498E-4	0.0	0.0	15.920738155839368	6.468242546158997E-5
29	3.234121273079498E-4	0.0	0.0	16.33619337457916	6.468242546158997E-5
30	3.8809455276953974E-4	0.0	0.0	16.818982998224467	6.468242546158997E-5
31	3.8809455276953974E-4	0.0	0.0	17.239095351597495	6.468242546158997E-5
32	4.5277697823112973E-4	0.0	0.0	17.652157320595208	6.468242546158997E-5
33	4.5277697823112973E-4	0.0	0.0	18.065672066571153	6.468242546158997E-5
34	4.5277697823112973E-4	0.0	0.0	18.462498746778007	6.468242546158997E-5
35	4.5277697823112973E-4	0.0	0.0	18.88565117414773	6.468242546158997E-5
36	4.5277697823112973E-4	0.0	0.0	19.28804054294428	6.468242546158997E-5
37	5.174594036927197E-4	0.0	0.0	19.687713249871443	6.468242546158997E-5
38	5.174594036927197E-4	0.0	0.0	20.089003017435147	6.468242546158997E-5
39	5.174594036927197E-4	0.0	0.0	20.54507879936482	6.468242546158997E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	45	3.8562575E-10	45.0	1
CGTTTTT	4420	0.0	40.978504	1
GCGTACG	105	0.0	40.714287	1
CATACCG	40	3.459245E-7	39.375004	1
AACACGT	600	0.0	39.375	41
GCGATCA	155	0.0	39.193546	9
ACGTTAG	75	0.0	39.0	1
GTAACGG	150	0.0	39.0	2
TAACGGG	480	0.0	38.906246	3
CGGGATA	530	0.0	38.207546	6
ACACGAC	635	0.0	37.559055	26
TCAAGCG	690	0.0	37.5	17
TCCGATT	30	1.1401043E-4	37.499996	30
CACGACC	640	0.0	37.26563	27
AAGGGCG	695	0.0	36.906475	5
ACGACCA	695	0.0	36.906475	28
ACGGGTA	250	0.0	36.899998	5
CGACAGG	135	0.0	36.666668	2
TGGGCGA	845	0.0	36.21302	6
TAGGGCG	560	0.0	36.160717	5
>>END_MODULE