FastQCFastQC Report
Sat 18 Jun 2016
SRR3553971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625155
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.3268611301691223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26200.1612153917626319No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC23270.14318634222581847Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC20120.12380357565893714No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT17270.10626678686033025Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17180.10571299352984792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG600.045.0000041
CTTAACG253.891522E-545.01
GCGCCTA207.03415E-445.041
ATTCGGT207.03415E-445.030
ATCGTAG253.891522E-545.01
TACGATC207.03415E-445.015
CGTAAGG3100.039.1935462
TACGTAG1050.038.571431
CGTTTTT36600.038.483611
TGCGACG950.037.8947371
TCGATAT301.14012975E-437.50000442
AGGTCGA301.14012975E-437.50000436
ATAACGC1450.037.2413811
TAGAACG800.036.56251
ACGGGAT6650.036.203015
CGTTCGA504.881076E-836.014
TACGCGA250.00210736536.021
CGTACGA250.00210736536.034
CTCGAAT250.00210736536.029
GCTACGA3350.035.59701510
Produced by FastQC (version 0.11.5)