##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1625155
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.792154594484835	31.0	31.0	34.0	30.0	34.0
2	31.91691315597589	33.0	31.0	34.0	30.0	34.0
3	31.909190815645278	33.0	31.0	34.0	30.0	34.0
4	35.5714211875175	37.0	35.0	37.0	33.0	37.0
5	35.421855761450445	37.0	35.0	37.0	33.0	37.0
6	35.369645972230344	37.0	35.0	37.0	33.0	37.0
7	35.72050789001664	37.0	35.0	37.0	35.0	37.0
8	35.59806603062477	37.0	35.0	37.0	35.0	37.0
9	37.34859997969424	39.0	37.0	39.0	34.0	39.0
10	36.70173675741699	39.0	37.0	39.0	32.0	39.0
11	36.61519424301067	39.0	35.0	39.0	32.0	39.0
12	36.571475951524626	39.0	35.0	39.0	32.0	39.0
13	36.5251517547557	39.0	35.0	39.0	32.0	39.0
14	37.619733502342854	39.0	36.0	41.0	32.0	41.0
15	37.73724167848605	40.0	36.0	41.0	33.0	41.0
16	37.75697333485114	40.0	36.0	41.0	33.0	41.0
17	37.683268980497246	39.0	36.0	41.0	33.0	41.0
18	37.656027271244895	39.0	36.0	41.0	33.0	41.0
19	37.586632659654	39.0	36.0	41.0	32.0	41.0
20	37.47046897065203	39.0	36.0	41.0	32.0	41.0
21	37.39890656583526	39.0	36.0	41.0	32.0	41.0
22	37.38010528226538	39.0	35.0	41.0	32.0	41.0
23	36.87975854610791	39.0	35.0	41.0	31.0	41.0
24	36.970948001882896	39.0	35.0	41.0	31.0	41.0
25	36.98319667970132	39.0	35.0	41.0	31.0	41.0
26	36.99324126006442	39.0	35.0	41.0	31.0	41.0
27	34.671041839086115	39.0	35.0	40.0	24.0	41.0
28	35.51903787638717	39.0	35.0	40.0	23.0	41.0
29	36.18122148348927	39.0	35.0	40.0	29.0	41.0
30	36.446056529992525	38.0	35.0	40.0	30.0	41.0
31	36.52705003522741	39.0	35.0	40.0	30.0	41.0
32	36.27938873522833	39.0	35.0	40.0	30.0	41.0
33	36.2956807196852	39.0	35.0	40.0	30.0	41.0
34	36.24425424036477	39.0	35.0	40.0	30.0	41.0
35	36.15198181096572	39.0	35.0	40.0	29.0	41.0
36	36.05267251431402	38.0	35.0	40.0	29.0	41.0
37	35.984025523719275	38.0	35.0	40.0	29.0	41.0
38	35.91477859034984	38.0	35.0	40.0	29.0	41.0
39	35.80754204983525	38.0	35.0	40.0	28.0	41.0
40	35.690035719669815	38.0	35.0	40.0	28.0	41.0
41	35.54712196682778	38.0	34.0	40.0	27.0	41.0
42	35.4757540050026	38.0	34.0	40.0	27.0	41.0
43	35.363683464038814	38.0	34.0	40.0	27.0	41.0
44	35.27118090274466	38.0	34.0	40.0	26.0	41.0
45	35.22379280745529	38.0	34.0	40.0	26.0	41.0
46	35.05289526229806	38.0	34.0	40.0	26.0	41.0
47	34.92275813691617	37.0	34.0	40.0	26.0	41.0
48	34.84662693712292	37.0	34.0	40.0	25.0	41.0
49	34.794147019822724	37.0	34.0	40.0	26.0	41.0
50	34.688399568041206	37.0	33.0	40.0	25.0	41.0
51	33.13184219351385	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	7.0
12	13.0
13	27.0
14	52.0
15	105.0
16	234.0
17	471.0
18	888.0
19	1574.0
20	2522.0
21	3923.0
22	5995.0
23	8103.0
24	11268.0
25	14766.0
26	18538.0
27	21823.0
28	25989.0
29	30989.0
30	37782.0
31	47528.0
32	60546.0
33	79808.0
34	116436.0
35	137229.0
36	155468.0
37	207802.0
38	294216.0
39	340984.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17554017924444	18.862077771043378	22.888216816242142	14.074165233470037
2	32.51892896369885	24.42296273278549	25.616510425159444	17.44159787835622
3	31.970304370967696	24.6961674424901	26.91687869772422	16.41664948881799
4	27.922382788103288	26.742310733437733	26.63893597841437	18.696370500044612
5	25.057117628780023	31.208161683039464	24.82347837590876	18.911242312271753
6	23.833603563967745	37.42006147105968	24.172463549630653	14.573871415341921
7	82.11579818540386	5.697610381778969	8.327821038608626	3.8587703942085523
8	83.71552251939046	5.123142100291972	7.231987102768659	3.929348277548911
9	77.49753100473494	7.2350021997901734	9.604991523885415	5.662475271589479
10	38.9241026240574	27.868295639492846	17.50553024173079	15.702071494718966
11	29.427100799616035	26.91367900292587	24.501047592383497	19.15817260507459
12	25.011706575680474	24.09308650559485	30.284742070756327	20.61046484796835
13	24.41514809356646	24.065889099808942	31.70786786491135	19.811094941713254
14	21.930708147838207	27.106029886380067	29.579886226236884	21.383375739544842
15	21.538130209118513	26.42750999135467	31.7288504788774	20.305509320649413
16	23.716445508274596	24.923961099095163	30.016521501026055	21.343071891604186
17	23.52981715590205	24.8170174537198	28.615793570459434	23.037371819918715
18	24.00374118161037	25.04542643624762	28.964621836071018	21.98621054607099
19	24.284575932757182	27.026775907528823	27.172485086037945	21.51616307367605
20	24.685399238841836	26.2839544535752	27.873895105389945	21.15675120219302
21	24.916823318391167	26.85288480175737	27.854943066968996	20.375348812882464
22	23.636391605723762	24.852398694278392	27.361636274693797	24.149573425304048
23	22.826622691374055	27.41424664108962	27.103260919727656	22.655869747808673
24	22.400632555048595	26.20851549544505	28.765563900058766	22.625288049447594
25	23.270026551313567	27.15740960093037	27.12264368629454	22.449920161461524
26	21.808381354393887	27.249154696013612	27.57164701213115	23.37081693746135
27	22.031190871024613	30.82352144872335	25.535595066316752	21.609692613935287
28	21.719220628186235	27.324101393405552	28.320990920866006	22.635687057542203
29	23.25224363214586	25.48390768880507	27.819377228633574	23.4444714504155
30	24.068165805723147	25.41419126175657	27.668253182004175	22.849389750516107
31	24.54024385366319	26.386283154529877	25.372656761970397	23.700816229836537
32	24.299159157126553	25.984475326968813	26.278724183231756	23.437641332672886
33	23.598179865920482	25.69391842624242	25.152308549030707	25.55559315880639
34	22.544065027643516	26.511194316849778	26.219468296870147	24.72527235863656
35	23.506988564167724	25.469878257766183	26.23220554347124	24.790927634594855
36	24.11837640102021	27.4733179296744	24.894240857025945	23.514064812279443
37	23.152745430435868	28.039786974165544	25.344413302115797	23.463054293282795
38	23.148068953422904	27.409139435930722	25.73465300232901	23.708138608317363
39	23.219877488608777	26.850546563250887	25.58322129273823	24.3463546554021
40	23.93334789604684	26.38917518636684	27.45978075937372	22.2176961582126
41	22.648178173774195	27.44476680685842	26.445354443114656	23.461700576252724
42	23.08462885078654	27.497377173254243	26.73591134384105	22.682082632118167
43	22.800594404841384	25.33530647845898	27.55890976553006	24.30518935116958
44	22.627626287954072	25.729668862354664	26.712898154329896	24.929806695361364
45	23.359494940482602	24.75419267700619	26.814796127138642	25.07151625537257
46	23.804744778190386	26.111601662610646	26.31736665118096	23.766286908018007
47	22.580184659309417	26.133630330645385	28.068891890312003	23.217293119733196
48	22.389310558069845	26.090803646421417	27.203559045137233	24.316326750371502
49	23.003282763797912	25.577314163879755	27.53405059825063	23.885352474071702
50	21.737741938461255	25.01644458528571	28.42971901141737	24.81609446483566
51	22.203297531620063	24.711242927597677	26.78778331912956	26.297676221652704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2965.0
1	3429.5
2	3894.0
3	7240.0
4	10586.0
5	7515.0
6	4444.0
7	4364.0
8	4284.0
9	4290.0
10	4296.0
11	4416.5
12	4537.0
13	4606.0
14	4675.0
15	4915.0
16	5155.0
17	4879.5
18	4604.0
19	4676.0
20	4748.0
21	5486.5
22	6225.0
23	6839.0
24	7453.0
25	9597.0
26	13542.5
27	15344.0
28	18028.5
29	20713.0
30	23818.0
31	26923.0
32	30861.0
33	34799.0
34	39171.5
35	43544.0
36	48467.5
37	53391.0
38	54583.0
39	55775.0
40	60440.0
41	65105.0
42	71098.0
43	77091.0
44	83379.0
45	89667.0
46	96198.0
47	102729.0
48	118151.5
49	133574.0
50	136941.5
51	140309.0
52	136253.0
53	132197.0
54	119288.0
55	106379.0
56	97767.5
57	89156.0
58	80789.5
59	72423.0
60	67515.0
61	62607.0
62	58684.0
63	54761.0
64	48666.0
65	42571.0
66	37251.5
67	31932.0
68	29104.5
69	26277.0
70	23837.5
71	21398.0
72	19138.0
73	16878.0
74	14395.0
75	9352.5
76	6793.0
77	5941.0
78	5089.0
79	4063.5
80	3038.0
81	2327.5
82	1617.0
83	1267.5
84	918.0
85	624.5
86	331.0
87	253.5
88	176.0
89	120.0
90	64.0
91	50.0
92	36.0
93	27.5
94	19.0
95	15.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1625155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.966973265678615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74166436846659	20.90298129225229
2	9.471478732126934	5.297771849756751
3	3.5438050362852116	2.973285021256972
4	1.8355112394886974	2.0533477505453206
5	1.1783359516631398	1.6477245029075498
6	0.7954782676209279	1.3348271666389697
7	0.6857316207121467	1.342448653272095
8	0.5335271823412062	1.1936912356039482
9	0.4539477823739978	1.1425990944300919
>10	5.6450815057549315	37.3380755360461
>50	1.017996145064822	18.62140126970045
>100	0.09036791303321422	3.8438831107786267
>500	0.004335833751488005	0.7992958295643924
>1k	0.0025102195403351613	1.1696498457702507
>5k	2.2820177639410553E-4	0.3390178414760502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5312	0.3268611301691223	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2620	0.1612153917626319	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2327	0.14318634222581847	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	2012	0.12380357565893714	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1727	0.10626678686033025	Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1718	0.10571299352984792	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4613036910325475E-4	0.0	0.0	0.11611200162446043	0.0
2	2.4613036910325475E-4	0.0	0.0	0.45503351987964225	0.0
3	2.4613036910325475E-4	0.0	0.0	0.6491688485098345	0.0
4	2.4613036910325475E-4	0.0	0.0	0.9101285723515603	0.0
5	2.4613036910325475E-4	0.0	0.0	1.6836547898508143	0.0
6	2.4613036910325475E-4	0.0	0.0	2.4333063615470523	0.0
7	2.4613036910325475E-4	0.0	0.0	3.042786688038987	0.0
8	2.4613036910325475E-4	0.0	0.0	4.1086542514406315	0.0
9	2.4613036910325475E-4	0.0	0.0	4.5771018764364015	0.0
10	2.4613036910325475E-4	0.0	0.0	5.573314545381825	0.0
11	2.4613036910325475E-4	0.0	0.0	6.590202165331922	0.0
12	2.4613036910325475E-4	0.0	0.0	7.4631650519488915	0.0
13	2.4613036910325475E-4	0.0	0.0	7.826330411560743	0.0
14	2.4613036910325475E-4	0.0	0.0	7.977762121151521	0.0
15	2.4613036910325475E-4	0.0	0.0	8.218846817688158	0.0
16	3.0766296137906847E-4	0.0	0.0	8.675480184966972	0.0
17	3.0766296137906847E-4	0.0	0.0	9.179739778667265	0.0
18	3.0766296137906847E-4	0.0	0.0	9.788543246644167	0.0
19	3.0766296137906847E-4	0.0	0.0	10.129249210075347	0.0
20	3.0766296137906847E-4	0.0	0.0	10.451803058785162	0.0
21	3.6919555365488215E-4	0.0	0.0	10.813676233959223	0.0
22	3.6919555365488215E-4	0.0	0.0	11.237266599185924	0.0
23	3.6919555365488215E-4	0.0	0.0	11.657534204429732	0.0
24	3.6919555365488215E-4	0.0	0.0	11.992271506410157	0.0
25	3.6919555365488215E-4	0.0	0.0	12.292427491531576	0.0
26	3.6919555365488215E-4	0.0	0.0	12.562309441253296	0.0
27	3.6919555365488215E-4	0.0	0.0	12.859204198984097	0.0
28	3.6919555365488215E-4	0.0	0.0	13.170805246268818	0.0
29	3.6919555365488215E-4	0.0	0.0	13.51132661192317	0.0
30	3.6919555365488215E-4	0.0	0.0	13.91393436318382	0.0
31	3.6919555365488215E-4	0.0	0.0	14.262393433241753	0.0
32	3.6919555365488215E-4	0.0	0.0	14.607591275909067	0.0
33	3.6919555365488215E-4	0.0	0.0	14.94725118527156	0.0
34	3.6919555365488215E-4	0.0	0.0	15.281434693921502	0.0
35	4.307281459306958E-4	0.0	0.0	15.643307869095564	0.0
36	4.307281459306958E-4	0.0	0.0	15.970168999264686	0.0
37	4.307281459306958E-4	0.0	0.0	16.323550676704684	0.0
38	4.307281459306958E-4	0.0	0.0	16.663333651251726	0.0
39	4.307281459306958E-4	0.0	0.0	17.010192873910487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	60	0.0	45.000004	1
CTTAACG	25	3.891522E-5	45.0	1
GCGCCTA	20	7.03415E-4	45.0	41
ATTCGGT	20	7.03415E-4	45.0	30
ATCGTAG	25	3.891522E-5	45.0	1
TACGATC	20	7.03415E-4	45.0	15
CGTAAGG	310	0.0	39.193546	2
TACGTAG	105	0.0	38.57143	1
CGTTTTT	3660	0.0	38.48361	1
TGCGACG	95	0.0	37.894737	1
TCGATAT	30	1.14012975E-4	37.500004	42
AGGTCGA	30	1.14012975E-4	37.500004	36
ATAACGC	145	0.0	37.24138	11
TAGAACG	80	0.0	36.5625	1
ACGGGAT	665	0.0	36.20301	5
CGTTCGA	50	4.881076E-8	36.0	14
TACGCGA	25	0.002107365	36.0	21
CGTACGA	25	0.002107365	36.0	34
CTCGAAT	25	0.002107365	36.0	29
GCTACGA	335	0.0	35.597015	10
>>END_MODULE