SRR3553970_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1236440
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2916	0.23583837468862218	RNA PCR Primer, Index 35 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2226	0.1800329979618906	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2047	0.16555595095597037	RNA PCR Primer, Index 10 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	2021	0.16345313965902106	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	1982	0.1602989227135971	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.1597327812105723	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	1912	0.15463750768334897	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1582	0.12794797968360777	TruSeq Adapter, Index 10 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATT	25	3.890977E-5	45.000004	9
CTAACGG	90	0.0	45.000004	2
CAATACG	25	3.890977E-5	45.000004	1
GTCGAAA	20	7.0334936E-4	45.0	15
ACGATAG	20	7.0334936E-4	45.0	1
ACGGATC	20	7.0334936E-4	45.0	5
AATTCGC	20	7.0334936E-4	45.0	13
TAGCCGT	65	0.0	41.53846	44
ATACCGG	95	0.0	40.26316	2
TTAACGG	85	0.0	39.705883	2
ATAGGGC	545	0.0	39.22018	4
CGTTAGG	155	0.0	39.193546	2
ATATCGA	35	6.2486743E-6	38.571426	38
TACCGGT	35	6.2486743E-6	38.571426	40
GTATGCG	65	9.094947E-12	38.07692	1
AATAGCG	65	9.094947E-12	38.07692	1
TAGGGAT	555	0.0	37.7027	5
CGTAAGG	185	0.0	37.7027	2
CACAACG	400	0.0	37.687504	12
ACGACCA	575	0.0	37.565216	28