##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1236440
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720935912781858	31.0	31.0	34.0	30.0	34.0
2	31.84242826178383	31.0	31.0	34.0	30.0	34.0
3	31.85721021642781	33.0	31.0	34.0	30.0	34.0
4	35.50642894115364	37.0	35.0	37.0	33.0	37.0
5	35.34871971142959	37.0	35.0	37.0	33.0	37.0
6	35.29847465303614	37.0	35.0	37.0	32.0	37.0
7	35.66337468862217	37.0	35.0	37.0	35.0	37.0
8	35.53193765973278	37.0	35.0	37.0	35.0	37.0
9	37.26145466015334	39.0	37.0	39.0	34.0	39.0
10	36.64927695642328	39.0	35.0	39.0	32.0	39.0
11	36.550558862540846	38.0	35.0	39.0	32.0	39.0
12	36.40764452783799	38.0	35.0	39.0	32.0	39.0
13	36.26275112419527	38.0	35.0	39.0	32.0	39.0
14	37.19131053670215	39.0	36.0	41.0	32.0	41.0
15	37.32763741063052	39.0	36.0	41.0	32.0	41.0
16	37.42372213775032	39.0	36.0	41.0	32.0	41.0
17	37.3552360001294	39.0	36.0	41.0	32.0	41.0
18	37.36136407751286	39.0	36.0	41.0	32.0	41.0
19	37.31890184723885	39.0	36.0	41.0	32.0	41.0
20	37.2206043156158	39.0	35.0	41.0	32.0	41.0
21	37.10547054446637	39.0	35.0	41.0	31.0	41.0
22	37.137824722590665	39.0	35.0	41.0	32.0	41.0
23	36.69734479635081	39.0	35.0	40.0	31.0	41.0
24	36.77521917763903	39.0	35.0	41.0	31.0	41.0
25	36.788760473617806	39.0	35.0	40.0	31.0	41.0
26	36.796783507489245	39.0	35.0	40.0	31.0	41.0
27	34.645724822878584	38.0	35.0	40.0	24.0	41.0
28	35.34928261783831	38.0	34.0	40.0	24.0	41.0
29	35.964272427291256	38.0	34.0	40.0	28.0	41.0
30	36.1876500274983	38.0	35.0	40.0	30.0	41.0
31	36.22884490957912	38.0	35.0	40.0	30.0	41.0
32	35.9971717188056	38.0	35.0	40.0	29.0	41.0
33	35.99399404742648	38.0	35.0	40.0	29.0	41.0
34	35.85623807058976	38.0	35.0	40.0	29.0	41.0
35	35.801725114037076	38.0	34.0	40.0	29.0	41.0
36	35.6639974442755	38.0	34.0	40.0	28.0	41.0
37	35.533018181229984	38.0	34.0	40.0	27.0	41.0
38	35.40356992656336	38.0	34.0	40.0	27.0	41.0
39	35.3218385040924	38.0	34.0	40.0	27.0	41.0
40	35.22164682475494	38.0	34.0	40.0	26.0	41.0
41	35.0682030668694	38.0	34.0	40.0	26.0	41.0
42	34.98531186309081	38.0	34.0	40.0	26.0	41.0
43	34.874162110575526	37.0	33.0	40.0	25.0	41.0
44	34.72309695577626	37.0	33.0	40.0	25.0	41.0
45	34.6326040891592	37.0	33.0	40.0	24.0	41.0
46	34.45598815955485	37.0	33.0	40.0	24.0	41.0
47	34.27740286629355	37.0	33.0	40.0	23.0	41.0
48	34.19917019831128	36.0	33.0	40.0	23.0	41.0
49	34.133950697162824	36.0	33.0	40.0	24.0	41.0
50	34.0102487787519	36.0	33.0	40.0	24.0	41.0
51	32.2281218660024	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	2.0
10	8.0
11	15.0
12	13.0
13	24.0
14	42.0
15	106.0
16	209.0
17	439.0
18	824.0
19	1370.0
20	2162.0
21	3409.0
22	4871.0
23	6761.0
24	9312.0
25	11971.0
26	15390.0
27	18910.0
28	22922.0
29	27361.0
30	33536.0
31	41078.0
32	51569.0
33	67490.0
34	98565.0
35	109822.0
36	117737.0
37	155727.0
38	211619.0
39	223135.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.719970237132415	18.19392772799327	22.52960111287244	13.556500922001877
2	31.558506680469733	25.18520914884669	25.00865387726052	18.247630293423054
3	33.28612791562874	24.871971142958817	25.28121057228818	16.56069036912426
4	28.232748859629258	26.337954126362785	25.90881886706998	19.520478146937982
5	25.122124809938214	30.455824787292553	24.609443240270455	19.81260716249879
6	24.828620879298633	36.560609491766684	23.60729190255896	15.003477726375724
7	81.85759114878198	5.789039500501439	7.959464268383424	4.39390508233315
8	82.67105561127107	5.412798033062663	7.156675617094238	4.75947073857203
9	76.56044773705155	7.040697486331727	9.532205363786355	6.866649412830384
10	41.405405842580315	25.355455986542008	16.996295817023068	16.242842353854613
11	32.28057972889909	26.40653812558636	21.646501245511306	19.666380900003237
12	27.84316262819061	22.724839054058428	28.363204037397686	21.068794280353274
13	26.1323638834072	23.7900747306784	30.037769725987513	20.039791659926887
14	22.185467956390927	26.374025427841218	29.863964284558897	21.576542331208955
15	21.339167286726408	24.97986153796383	32.55184238620556	21.1291287891042
16	25.276196176118532	23.813367409659993	29.17416130180195	21.736275112419527
17	24.17488919802012	24.15758144349908	27.302012228656462	24.365517129824337
18	25.790252660865065	23.566934101129046	28.356652971434116	22.28616026657177
19	25.03493901847239	26.54653683154864	25.845977160234217	22.57254698974475
20	26.091439940474263	26.229659344569896	26.15808288311604	21.520817831839796
21	24.717252757917894	27.642020639901656	27.200349390184726	20.44037721199573
22	23.198861246805343	24.042573841027465	28.064038691727866	24.694526220439325
23	22.289152728802044	27.671298243343795	26.805667885218853	23.23388114263531
24	23.24253501989583	25.643622011581634	27.804341496554624	23.30950147196791
25	23.72731390120022	26.332777975477985	25.375190061790303	24.564718061531494
26	22.73252240302805	25.57455274821261	27.01732392999256	24.675600918766783
27	22.350053379056	29.354922195982013	25.58627996506098	22.708744459901006
28	21.263061693248357	26.933373232829737	28.178075765908577	23.62548930801333
29	25.002830707515123	26.338843777296105	25.850182782828117	22.808142732360647
30	25.64807026624826	24.68174759794248	27.697259875125358	21.972922260683898
31	26.842143573485167	24.86226586004982	24.963443434376114	23.332147132088902
32	26.977451392708097	25.234059072821974	26.047200025880755	21.741289508589176
33	25.981608488887453	24.242987933098252	25.55716411633399	24.21823946168031
34	24.34562129986089	24.378700139109057	28.924735531040728	22.350943029989324
35	24.53083044870758	24.818430332244183	27.996829607583063	22.653909611465174
36	26.091601695189414	26.318462683187217	26.32574164536896	21.264193976254408
37	24.41388178965417	26.256995891430236	27.558312574811556	21.770809744104042
38	23.097602795121478	26.484423020931057	28.445779819481736	21.972194364465725
39	24.390912620102874	22.982918702080166	27.870903561838826	24.75526511597813
40	25.697405454368994	22.74101452557342	29.093930963087573	22.46764905697001
41	23.06792080489146	23.968490181488793	27.30613697389279	25.65745203972696
42	23.702403675067128	24.203843292031962	27.392756623855586	24.700996409045324
43	23.416987480185046	22.78444566659118	28.393694801203456	25.404872052020316
44	23.62516579858303	23.361667367603765	28.253129953738153	24.760036880075052
45	24.65554333408819	22.56567241435088	27.99092556048009	24.787858691080842
46	24.8497298696257	23.922713597101357	27.389925916340463	23.837630616932483
47	22.626168677816956	24.766749700753778	29.220665782407558	23.38641583902171
48	22.42162984050985	24.813092426644236	28.7037785901459	24.06149914270001
49	24.290543819352333	23.53183332794151	28.5709779690078	23.606644883698362
50	22.52393969784219	23.441493319530267	28.992429879330984	25.042137103296565
51	22.56737083885995	23.19295719970237	27.491265245381904	26.748406716055772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2554.0
1	2887.0
2	3220.0
3	4028.5
4	4837.0
5	3493.5
6	2150.0
7	2123.0
8	2096.0
9	2252.5
10	2409.0
11	2416.0
12	2423.0
13	2439.0
14	2455.0
15	2535.5
16	2616.0
17	2629.0
18	2642.0
19	2875.5
20	3109.0
21	3182.5
22	3256.0
23	3494.5
24	3733.0
25	4769.0
26	6747.5
27	7690.0
28	10072.5
29	12455.0
30	14374.5
31	16294.0
32	18448.5
33	20603.0
34	23197.5
35	25792.0
36	29156.0
37	32520.0
38	34482.0
39	36444.0
40	41504.0
41	46564.0
42	50497.5
43	54431.0
44	62712.5
45	70994.0
46	85325.0
47	99656.0
48	107821.5
49	115987.0
50	115807.5
51	115628.0
52	106724.0
53	97820.0
54	88822.5
55	79825.0
56	73630.5
57	67436.0
58	64648.0
59	61860.0
60	58883.5
61	55907.0
62	51076.0
63	46245.0
64	40347.5
65	34450.0
66	30642.5
67	26835.0
68	23969.0
69	21103.0
70	18183.5
71	15264.0
72	13562.0
73	11860.0
74	9902.0
75	6347.0
76	4750.0
77	4036.5
78	3323.0
79	2477.0
80	1631.0
81	1217.5
82	804.0
83	640.0
84	476.0
85	358.0
86	240.0
87	182.0
88	124.0
89	113.5
90	103.0
91	71.0
92	39.0
93	27.0
94	15.0
95	13.5
96	12.0
97	10.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1236440.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.642420260615438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.54388413821115	23.903913300205183
2	10.048708367341465	6.359309064715629
3	3.8701229941327804	3.673801747218624
4	1.9526990631464305	2.4715249759435762
5	1.1515043287593107	1.8218191951259997
6	0.7487077646752683	1.4214555445344483
7	0.54526366005708	1.2077423319057234
8	0.4274861363952515	1.0821356786724259
9	0.36949502068597945	1.05225450558755
>10	4.39425473220997	33.65434389725243
>50	0.8797197675805921	17.97476079727701
>100	0.06386284734844948	3.502250771708076
>500	0.0017669562466928667	0.3765191140969199
>1k	0.002524223209561238	1.4981690757564545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2916	0.23583837468862218	RNA PCR Primer, Index 35 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2226	0.1800329979618906	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2047	0.16555595095597037	RNA PCR Primer, Index 10 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	2021	0.16345313965902106	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	1982	0.1602989227135971	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.1597327812105723	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	1912	0.15463750768334897	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1582	0.12794797968360777	TruSeq Adapter, Index 10 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.087735757497331E-5	0.0	0.0	0.14590275306525186	0.0
2	8.087735757497331E-5	0.0	0.0	0.7197276050596875	0.0
3	8.087735757497331E-5	0.0	0.0	0.9691533758209052	0.0
4	8.087735757497331E-5	0.0	0.0	1.305684060690369	0.0
5	8.087735757497331E-5	0.0	0.0	2.3469800394681504	0.0
6	8.087735757497331E-5	0.0	0.0	3.1525185209148847	0.0
7	8.087735757497331E-5	0.0	0.0	3.879120701368445	0.0
8	8.087735757497331E-5	0.0	0.0	5.085406489599172	0.0
9	8.087735757497331E-5	0.0	0.0	5.6805829639934	0.0
10	8.087735757497331E-5	0.0	0.0	7.024198505386432	0.0
11	8.087735757497331E-5	0.0	0.0	8.377761961761186	0.0
12	8.087735757497331E-5	0.0	0.0	9.59019442916761	0.0
13	8.087735757497331E-5	0.0	0.0	10.034453754326938	0.0
14	8.087735757497331E-5	0.0	0.0	10.223787648409951	0.0
15	8.087735757497331E-5	0.0	0.0	10.547782342855294	0.0
16	8.087735757497331E-5	0.0	0.0	11.157112354825143	0.0
17	8.087735757497331E-5	0.0	0.0	11.85031218660024	0.0
18	8.087735757497331E-5	0.0	0.0	12.659409271780273	0.0
19	1.6175471514994662E-4	0.0	0.0	13.109330011969849	0.0
20	1.6175471514994662E-4	0.0	0.0	13.535311054317233	0.0
21	1.6175471514994662E-4	0.0	0.0	14.010789039500501	0.0
22	2.4263207272491992E-4	0.0	0.0	14.507699524441138	0.0
23	2.4263207272491992E-4	0.0	0.0	14.993691566109153	0.0
24	2.4263207272491992E-4	0.0	0.0	15.403173627511242	0.0
25	4.0438678787486654E-4	0.0	0.0	15.758386981980525	0.0
26	4.0438678787486654E-4	0.0	0.0	16.092976610268188	0.0
27	5.661415030248131E-4	0.0	0.0	16.499951473585455	0.0
28	5.661415030248131E-4	0.0	0.0	16.875707676878783	0.0
29	5.661415030248131E-4	0.0	0.0	17.276131474232475	0.0
30	5.661415030248131E-4	0.0	0.0	17.764145449839862	0.0
31	5.661415030248131E-4	0.0	0.0	18.156643266151207	0.0
32	5.661415030248131E-4	0.0	0.0	18.5577140823655	0.0
33	5.661415030248131E-4	0.0	0.0	18.966387370191843	0.0
34	5.661415030248131E-4	0.0	0.0	19.36236291287891	0.0
35	5.661415030248131E-4	0.0	0.0	19.7766976157355	0.0
36	5.661415030248131E-4	0.0	0.0	20.16927630940442	0.0
37	6.470188605997865E-4	0.0	0.0	20.563553427582413	0.0
38	6.470188605997865E-4	0.0	0.0	21.01541522435379	0.0
39	6.470188605997865E-4	0.0	0.0	21.645611594577982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATT	25	3.890977E-5	45.000004	9
CTAACGG	90	0.0	45.000004	2
CAATACG	25	3.890977E-5	45.000004	1
GTCGAAA	20	7.0334936E-4	45.0	15
ACGATAG	20	7.0334936E-4	45.0	1
ACGGATC	20	7.0334936E-4	45.0	5
AATTCGC	20	7.0334936E-4	45.0	13
TAGCCGT	65	0.0	41.53846	44
ATACCGG	95	0.0	40.26316	2
TTAACGG	85	0.0	39.705883	2
ATAGGGC	545	0.0	39.22018	4
CGTTAGG	155	0.0	39.193546	2
ATATCGA	35	6.2486743E-6	38.571426	38
TACCGGT	35	6.2486743E-6	38.571426	40
GTATGCG	65	9.094947E-12	38.07692	1
AATAGCG	65	9.094947E-12	38.07692	1
TAGGGAT	555	0.0	37.7027	5
CGTAAGG	185	0.0	37.7027	2
CACAACG	400	0.0	37.687504	12
ACGACCA	575	0.0	37.565216	28
>>END_MODULE