Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553968_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1795406 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC | 3967 | 0.2209528095595091 | TruSeq Adapter, Index 13 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC | 3494 | 0.19460779344616203 | TruSeq Adapter, Index 13 (95% over 21bp) |
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC | 3144 | 0.17511359547645491 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3053 | 0.17004510400433107 | No Hit |
AGTGAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG | 2924 | 0.16286009960978187 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG | 2909 | 0.16202463398250871 | No Hit |
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT | 2485 | 0.13840880558492064 | Illumina Single End Adapter 1 (95% over 21bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC | 2444 | 0.13612519953704064 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2022 | 0.11262076655642232 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TACGTCG | 35 | 1.2124474E-7 | 45.000004 | 1 |
GTCAAGC | 785 | 0.0 | 39.554142 | 16 |
AAATGCG | 40 | 3.4596633E-7 | 39.375 | 1 |
ACACGTG | 725 | 0.0 | 39.103447 | 42 |
TAGTGCG | 190 | 0.0 | 39.07895 | 1 |
CTACGTT | 35 | 6.2502513E-6 | 38.57143 | 39 |
CACGACC | 750 | 0.0 | 38.4 | 27 |
GCGAGAC | 750 | 0.0 | 38.100002 | 21 |
ATAACGG | 95 | 0.0 | 37.894737 | 2 |
AACACGT | 745 | 0.0 | 37.75168 | 41 |
AATGCGT | 30 | 1.14017734E-4 | 37.500004 | 34 |
ACGATAG | 30 | 1.14017734E-4 | 37.500004 | 1 |
TATCGCG | 60 | 1.5643309E-10 | 37.500004 | 1 |
ACTACGG | 90 | 0.0 | 37.5 | 2 |
ACGACCA | 790 | 0.0 | 37.310123 | 28 |
TCGTTTA | 115 | 0.0 | 37.173912 | 38 |
CGTCATA | 85 | 0.0 | 37.058823 | 38 |
CACAACG | 555 | 0.0 | 36.89189 | 12 |
GACACGA | 785 | 0.0 | 36.6879 | 25 |
AGTGAAT | 1895 | 0.0 | 36.451187 | 1 |