FastQCFastQC Report
Sat 18 Jun 2016
SRR3553968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795406
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC39670.2209528095595091TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC34940.19460779344616203TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC31440.17511359547645491No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.17004510400433107No Hit
AGTGAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG29240.16286009960978187No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG29090.16202463398250871No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT24850.13840880558492064Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC24440.13612519953704064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20220.11262076655642232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG351.2124474E-745.0000041
GTCAAGC7850.039.55414216
AAATGCG403.4596633E-739.3751
ACACGTG7250.039.10344742
TAGTGCG1900.039.078951
CTACGTT356.2502513E-638.5714339
CACGACC7500.038.427
GCGAGAC7500.038.10000221
ATAACGG950.037.8947372
AACACGT7450.037.7516841
AATGCGT301.14017734E-437.50000434
ACGATAG301.14017734E-437.5000041
TATCGCG601.5643309E-1037.5000041
ACTACGG900.037.52
ACGACCA7900.037.31012328
TCGTTTA1150.037.17391238
CGTCATA850.037.05882338
CACAACG5550.036.8918912
GACACGA7850.036.687925
AGTGAAT18950.036.4511871