##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1795406
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675612089967395	31.0	31.0	34.0	30.0	34.0
2	31.806764598090904	31.0	31.0	34.0	30.0	34.0
3	31.803280706425177	31.0	31.0	34.0	30.0	34.0
4	35.468257318957384	37.0	35.0	37.0	33.0	37.0
5	35.30661087241549	37.0	35.0	37.0	33.0	37.0
6	35.25514173395878	37.0	35.0	37.0	32.0	37.0
7	35.631888831829684	37.0	35.0	37.0	35.0	37.0
8	35.50962122216368	37.0	35.0	37.0	33.0	37.0
9	37.223568930927044	39.0	37.0	39.0	34.0	39.0
10	36.60782742176421	38.0	35.0	39.0	32.0	39.0
11	36.51652662406163	38.0	35.0	39.0	32.0	39.0
12	36.42000528014277	38.0	35.0	39.0	32.0	39.0
13	36.32142813380372	38.0	35.0	39.0	32.0	39.0
14	37.28770818410989	39.0	36.0	41.0	32.0	41.0
15	37.42441820958602	39.0	36.0	41.0	32.0	41.0
16	37.46576317557143	39.0	36.0	41.0	32.0	41.0
17	37.39720709410573	39.0	36.0	41.0	32.0	41.0
18	37.37776023918824	39.0	36.0	41.0	32.0	41.0
19	37.36724896764297	39.0	36.0	41.0	32.0	41.0
20	37.24954467123314	39.0	35.0	41.0	32.0	41.0
21	37.16363931055149	39.0	35.0	41.0	31.0	41.0
22	37.17618354845645	39.0	35.0	41.0	32.0	41.0
23	36.6991131810855	39.0	35.0	40.0	31.0	41.0
24	36.79761235063267	39.0	35.0	41.0	31.0	41.0
25	36.789321746724696	39.0	35.0	40.0	31.0	41.0
26	36.785156115107114	39.0	35.0	40.0	31.0	41.0
27	34.57982038602968	38.0	35.0	40.0	24.0	41.0
28	35.35718829055935	38.0	34.0	40.0	23.0	41.0
29	35.94522743045306	38.0	34.0	40.0	27.0	41.0
30	36.16405704336512	38.0	35.0	40.0	30.0	41.0
31	36.22952301596408	38.0	35.0	40.0	30.0	41.0
32	36.04804038752238	38.0	35.0	40.0	29.0	41.0
33	36.05780920861354	38.0	35.0	40.0	29.0	41.0
34	36.002263554872826	38.0	35.0	40.0	29.0	41.0
35	35.91156763428439	38.0	35.0	40.0	29.0	41.0
36	35.811570753356065	38.0	35.0	40.0	29.0	41.0
37	35.736405581801556	38.0	34.0	40.0	28.0	41.0
38	35.64868391884621	38.0	34.0	40.0	28.0	41.0
39	35.58288821581303	38.0	34.0	40.0	27.0	41.0
40	35.48069963005582	38.0	34.0	40.0	27.0	41.0
41	35.36351499326615	38.0	34.0	40.0	27.0	41.0
42	35.29862771985835	38.0	34.0	40.0	27.0	41.0
43	35.20141238249176	38.0	34.0	40.0	26.0	41.0
44	35.065879249595916	38.0	34.0	40.0	26.0	41.0
45	34.99094299562327	37.0	34.0	40.0	26.0	41.0
46	34.84196666380752	37.0	33.0	40.0	26.0	41.0
47	34.68844094316272	37.0	33.0	40.0	25.0	41.0
48	34.61480912952279	37.0	33.0	40.0	25.0	41.0
49	34.57683498885489	37.0	33.0	40.0	25.0	41.0
50	34.451852115900245	37.0	33.0	40.0	24.0	41.0
51	32.82797651339028	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	8.0
11	14.0
12	18.0
13	19.0
14	59.0
15	113.0
16	234.0
17	505.0
18	1006.0
19	1774.0
20	3012.0
21	4794.0
22	6976.0
23	9810.0
24	12849.0
25	16280.0
26	20576.0
27	25900.0
28	31112.0
29	38058.0
30	46646.0
31	58559.0
32	72640.0
33	96244.0
34	138572.0
35	154682.0
36	172994.0
37	228572.0
38	314846.0
39	338476.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65386213480405	19.701393445270874	23.514514265854075	14.130230154071002
2	31.562276164834024	25.198311691060404	24.82391169462506	18.415500449480508
3	32.368277704318686	24.62245308303526	26.424441045646503	16.584828166999554
4	29.734555860902773	25.580509366683636	25.409461703926578	19.275473068487017
5	25.023810770377285	30.942026483146428	23.747386385029348	20.286776361446936
6	24.006213636358574	36.84848997942527	23.932247079490658	15.213049304725503
7	80.99232151390827	5.491069986398619	8.913805568211313	4.602802931481793
8	82.88782592906563	5.009451901129883	7.179323228283742	4.923398941520748
9	76.30909109137431	7.292946553592892	9.685385923852321	6.7125764311804685
10	39.77813374802134	24.677482419018318	18.052462785576076	17.491921047384267
11	30.42375930569465	26.043691510443878	23.163618702399347	20.368930481462133
12	25.976185887760206	21.926516899241733	29.67629605782759	22.421001155170476
13	25.591481815255158	22.747612517725795	31.158690569152604	20.502215097866443
14	21.987004610656307	26.013893236404467	29.93105737643742	22.068044776501804
15	21.10876314326676	24.27200310124841	32.35095571697989	22.268278038504942
16	24.35861303794239	23.395154076570982	29.532206085977208	22.714026799509416
17	23.422111767477663	24.11705207624348	27.858100062047246	24.602736094231613
18	24.044032380419804	23.730565677066913	29.38237925015289	22.84302269236039
19	24.775844572202608	25.888907578564403	26.428841164616806	22.906406684616183
20	24.920714311971775	26.203432538378507	26.569756367083546	22.306096782566172
21	24.918987683008744	26.36757368528344	27.667391108195027	21.046047523512787
22	23.357279634801266	23.820127592310598	26.79193452622972	26.030658246658415
23	22.458095829021403	26.89347144879765	27.160597658691128	23.48783506348982
24	22.882233879133747	25.636151377460024	27.884277985035137	23.59733675837109
25	23.85532854407304	25.511110021911477	26.037230576259628	24.59633085775585
26	21.912035495035664	25.38857506324475	26.975235684853455	25.724153756866137
27	21.996083337139343	28.54474141224882	26.14840320239545	23.310772048216393
28	21.496196403487566	25.598054144856373	28.487372772509396	24.418376679146668
29	24.350536870212085	24.77105456927291	27.02374838894378	23.854660171571222
30	25.26765533812408	23.953078022464	27.884389380452106	22.894877258959813
31	24.951849331014824	24.764036658003814	26.353370769619794	23.93074324136156
32	26.01227800285841	24.5379596592637	25.737242718360083	23.712519619517813
33	24.21892318506232	23.834107717140302	26.06897827009601	25.877990827701367
34	22.824698146268865	24.155594890515015	28.023188069996426	24.996518893219697
35	23.966501170208858	23.429686655831606	27.336156835835464	25.26765533812408
36	23.7409254508451	25.41324914810355	26.39297184035254	24.452853560698806
37	23.193639767272696	25.045922760645784	27.32395903767727	24.43647843440425
38	22.88401620580526	25.29968152050288	27.223647464696004	24.59265480899585
39	23.820238987727567	22.87900341204162	26.177867290183947	27.122890310046866
40	24.126242198143483	22.363242631471657	28.358878159034777	25.151637011350083
41	21.026386232417625	23.310103675714576	27.12567519547111	28.537834896396692
42	22.594109633141475	24.09900601869438	26.800177787085484	26.506706561078662
43	22.573000201625703	22.454196989427462	28.93529374414478	26.037509064802055
44	23.28721191752729	23.036850717887763	28.21501097801834	25.4609263865666
45	24.25351146203143	21.85695046134412	27.191955468568114	26.69758260805634
46	23.72455032455055	22.932027630519226	27.36929697238396	25.97412507254627
47	21.52326548981122	23.786430478677246	29.206430189049158	25.483873842462373
48	21.427966710593594	23.81205142458029	28.700639298297993	26.059342566528127
49	23.90361845732943	22.808434415391282	28.800505289611372	24.487441837667916
50	22.214418354400063	22.254632099926145	29.686377343063352	25.84457220261044
51	21.862520232192605	22.3991676534444	27.31092577389181	28.427386340471177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2285.0
1	2896.5
2	3508.0
3	5033.5
4	6559.0
5	4827.0
6	3095.0
7	3063.0
8	3031.0
9	3006.0
10	2981.0
11	3026.5
12	3072.0
13	3117.5
14	3163.0
15	3138.5
16	3114.0
17	3319.0
18	3524.0
19	3592.0
20	3660.0
21	4257.5
22	4855.0
23	4961.5
24	5068.0
25	6642.5
26	10019.5
27	11822.0
28	15358.0
29	18894.0
30	21502.0
31	24110.0
32	27270.0
33	30430.0
34	34525.0
35	38620.0
36	43323.0
37	48026.0
38	49508.5
39	50991.0
40	56469.5
41	61948.0
42	67812.0
43	73676.0
44	84616.0
45	95556.0
46	107178.0
47	118800.0
48	139745.5
49	160691.0
50	166970.5
51	173250.0
52	171198.5
53	169147.0
54	152227.5
55	135308.0
56	120909.5
57	106511.0
58	97803.0
59	89095.0
60	83376.0
61	77657.0
62	71044.5
63	64432.0
64	58509.0
65	52586.0
66	45945.0
67	39304.0
68	34339.5
69	29375.0
70	26108.5
71	22842.0
72	19678.0
73	16514.0
74	14553.5
75	10343.0
76	8093.0
77	6148.5
78	4204.0
79	3275.0
80	2346.0
81	1787.5
82	1229.0
83	884.0
84	539.0
85	427.0
86	315.0
87	236.5
88	158.0
89	126.0
90	94.0
91	81.5
92	69.0
93	45.5
94	22.0
95	20.0
96	18.0
97	11.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1795406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.87660204110976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0989582125373	20.65627169751724
2	10.364533810416749	5.778559687492293
3	3.9835055917958764	3.3313980033308725
4	2.037747589047947	2.272219144004819
5	1.2045772686688927	1.6789760573224837
6	0.827811743276589	1.3845947119367275
7	0.5751297852747668	1.1222864902265506
8	0.46744714782724534	1.0424670492185537
9	0.3911664767899722	0.9813952984767345
>10	4.752844410322034	32.27009087032114
>50	1.1895095324838882	21.886605124535162
>100	0.09726873220768133	4.249891928401564
>500	0.0055759104887064944	1.0433306235658402
>1k	0.003923788862423089	2.301913313650064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	3967	0.2209528095595091	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	3494	0.19460779344616203	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	3144	0.17511359547645491	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3053	0.17004510400433107	No Hit
AGTGAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG	2924	0.16286009960978187	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG	2909	0.16202463398250871	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2485	0.13840880558492064	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	2444	0.13612519953704064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2022	0.11262076655642232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1139541696975503E-4	0.0	0.0	0.15121927853644246	0.0
2	1.1139541696975503E-4	0.0	0.0	0.7329818436609881	0.0
3	1.6709312545463254E-4	0.0	0.0	1.074074610422378	0.0
4	1.6709312545463254E-4	0.0	0.0	1.4400085551680233	0.0
5	1.6709312545463254E-4	0.0	0.0	2.6149517156565145	0.0
6	1.6709312545463254E-4	0.0	0.0	3.59010719580975	0.0
7	1.6709312545463254E-4	0.0	0.0	4.424904450581094	0.0
8	1.6709312545463254E-4	0.0	0.0	5.815899022282426	0.0
9	1.6709312545463254E-4	0.0	0.0	6.412421480155463	0.0
10	1.6709312545463254E-4	0.0	0.0	7.707615993262805	0.0
11	1.6709312545463254E-4	0.0	0.0	9.184830617698726	0.0
12	1.6709312545463254E-4	0.0	0.0	10.39976473287936	0.0
13	1.6709312545463254E-4	0.0	0.0	11.044744197134241	0.0
14	1.6709312545463254E-4	0.0	0.0	11.236901291407069	0.0
15	2.2279083393951006E-4	0.0	0.0	11.656750617966075	0.0
16	2.2279083393951006E-4	0.0	0.0	12.269091225048818	0.0
17	2.2279083393951006E-4	0.0	0.0	12.977621774684946	0.0
18	2.2279083393951006E-4	0.0	0.0	13.867392667730865	0.0
19	2.2279083393951006E-4	0.0	0.0	14.322665736886252	0.0
20	2.784885424243876E-4	0.0	0.0	14.737669362807075	0.0
21	2.784885424243876E-4	0.0	0.0	15.211322675762474	0.0
22	2.784885424243876E-4	0.0	0.0	15.775930346673677	0.0
23	2.784885424243876E-4	0.0	0.0	16.315585444183654	0.0
24	2.784885424243876E-4	0.0	0.0	16.741171634716604	0.0
25	2.784885424243876E-4	0.0	0.0	17.124427566801046	0.0
26	3.341862509092651E-4	0.0	0.0	17.47103440670244	0.0
27	3.341862509092651E-4	0.0	0.0	17.86843755674204	0.0
28	3.341862509092651E-4	0.0	0.0	18.25041244153133	0.0
29	3.341862509092651E-4	0.0	0.0	18.652104315124266	0.0
30	3.341862509092651E-4	0.0	0.0	19.121803090777238	0.0
31	3.341862509092651E-4	0.0	0.0	19.502496928271377	0.0
32	3.341862509092651E-4	0.0	0.0	19.903854615613405	0.0
33	3.341862509092651E-4	0.0	0.0	20.301480556486943	0.0
34	3.8988395939414263E-4	0.0	0.0	20.70885359634534	0.0
35	3.8988395939414263E-4	0.0	0.0	21.145467933158294	0.0
36	4.455816678790201E-4	0.0	0.0	21.51563490374879	0.0
37	4.455816678790201E-4	0.0	0.0	21.924734572570216	0.0
38	4.455816678790201E-4	0.0	0.0	22.32765179574982	0.0
39	5.012793763638976E-4	0.0	0.0	22.853215373013125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCG	35	1.2124474E-7	45.000004	1
GTCAAGC	785	0.0	39.554142	16
AAATGCG	40	3.4596633E-7	39.375	1
ACACGTG	725	0.0	39.103447	42
TAGTGCG	190	0.0	39.07895	1
CTACGTT	35	6.2502513E-6	38.57143	39
CACGACC	750	0.0	38.4	27
GCGAGAC	750	0.0	38.100002	21
ATAACGG	95	0.0	37.894737	2
AACACGT	745	0.0	37.75168	41
AATGCGT	30	1.14017734E-4	37.500004	34
ACGATAG	30	1.14017734E-4	37.500004	1
TATCGCG	60	1.5643309E-10	37.500004	1
ACTACGG	90	0.0	37.5	2
ACGACCA	790	0.0	37.310123	28
TCGTTTA	115	0.0	37.173912	38
CGTCATA	85	0.0	37.058823	38
CACAACG	555	0.0	36.89189	12
GACACGA	785	0.0	36.6879	25
AGTGAAT	1895	0.0	36.451187	1
>>END_MODULE