FastQCFastQC Report
Sat 18 Jun 2016
SRR3553965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698617
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC32990.4722186834846561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23890.341961332174854No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC23640.3383828335124968No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG22300.31920208068226225No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC21780.31175880346455925No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC20450.29272119058081897No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT19480.27883661577087304Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14510.20769606236321186No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.14686158510313949No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC8470.12123953468066194No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC8400.12023755505520192No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT8130.11637277649985615No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA207.0313836E-445.00000419
TCGTTGC406.8084773E-945.00000416
CGACGGT302.1642736E-645.00000428
ACGCATC351.2110104E-745.00000410
CGCGATT253.8892285E-545.00000430
TTGCGTA207.0313836E-445.00000414
GGACGAT207.0313836E-445.0000048
TATAGCG502.1827873E-1145.0000041
CGGTCGA351.2110104E-745.00000441
GCTACGA302.1642736E-645.00000410
AATCGTG253.8892285E-545.00000414
CGTAAGG850.042.352942
ATTGCGG800.042.1875042
TCGTTTA650.041.53846438
ACGCATT650.041.53846417
TACGCAT650.041.53846416
TATTAGT501.0804797E-940.50000430
GTTGCGC501.0804797E-940.5000049
CGTTGCG451.9264917E-840.017
GTTAGCG900.040.01