##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698617
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.688689224567966	31.0	31.0	34.0	30.0	34.0
2	31.800573132345765	31.0	31.0	34.0	30.0	34.0
3	31.811882619518276	31.0	31.0	34.0	30.0	34.0
4	35.485380401564804	37.0	35.0	37.0	33.0	37.0
5	35.32464855564637	37.0	35.0	37.0	33.0	37.0
6	35.271762639615126	37.0	35.0	37.0	32.0	37.0
7	35.60456730941274	37.0	35.0	37.0	35.0	37.0
8	35.419359964043245	37.0	35.0	37.0	33.0	37.0
9	37.17566420513672	39.0	37.0	39.0	34.0	39.0
10	36.62656076219159	38.0	35.0	39.0	32.0	39.0
11	36.573145228358314	38.0	35.0	39.0	32.0	39.0
12	36.48689339079925	38.0	35.0	39.0	32.0	39.0
13	36.33046003747404	38.0	35.0	39.0	32.0	39.0
14	37.37980037703062	39.0	36.0	41.0	32.0	41.0
15	37.49316864605356	39.0	36.0	41.0	32.0	41.0
16	37.57618695222132	39.0	36.0	41.0	32.0	41.0
17	37.52706275398394	39.0	36.0	41.0	32.0	41.0
18	37.52531072103885	39.0	36.0	41.0	32.0	41.0
19	37.40771123519754	39.0	36.0	41.0	32.0	41.0
20	37.283981065447875	39.0	35.0	41.0	32.0	41.0
21	37.212481230774515	39.0	35.0	41.0	32.0	41.0
22	37.21482013750023	39.0	35.0	41.0	32.0	41.0
23	36.773190460581404	39.0	35.0	40.0	31.0	41.0
24	36.878570089190504	39.0	35.0	41.0	31.0	41.0
25	36.8749443543458	39.0	35.0	40.0	31.0	41.0
26	36.83965606333656	39.0	35.0	40.0	31.0	41.0
27	34.72057364764957	38.0	35.0	40.0	25.0	41.0
28	35.457322109253	38.0	35.0	40.0	24.0	41.0
29	36.052998996588975	38.0	35.0	40.0	29.0	41.0
30	36.281229915676256	38.0	35.0	40.0	30.0	41.0
31	36.288617368314824	38.0	35.0	40.0	30.0	41.0
32	36.048246750365365	38.0	35.0	40.0	30.0	41.0
33	36.065656862057466	38.0	35.0	40.0	30.0	41.0
34	35.94697810101959	38.0	35.0	40.0	29.0	41.0
35	35.90472891441233	38.0	35.0	40.0	29.0	41.0
36	35.797724647410526	38.0	35.0	40.0	29.0	41.0
37	35.67532854196219	38.0	34.0	40.0	29.0	41.0
38	35.633736367709346	38.0	34.0	40.0	28.0	41.0
39	35.5217035943872	38.0	34.0	40.0	28.0	41.0
40	35.384727253988956	38.0	34.0	40.0	27.0	41.0
41	35.26216367480322	38.0	34.0	40.0	27.0	41.0
42	35.16189843648237	38.0	34.0	40.0	26.0	41.0
43	35.021074494322356	37.0	34.0	40.0	26.0	41.0
44	34.92595084287957	37.0	34.0	40.0	26.0	41.0
45	34.90523133562453	37.0	34.0	40.0	26.0	41.0
46	34.76411252517474	37.0	33.0	40.0	25.0	41.0
47	34.61778628347149	37.0	33.0	40.0	25.0	41.0
48	34.56476152169214	37.0	33.0	40.0	25.0	41.0
49	34.50010234506174	37.0	33.0	40.0	25.0	41.0
50	34.368174550576356	37.0	33.0	40.0	24.0	41.0
51	32.68686562164963	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	8.0
12	5.0
13	8.0
14	24.0
15	33.0
16	82.0
17	209.0
18	350.0
19	626.0
20	1179.0
21	1792.0
22	2610.0
23	3663.0
24	4775.0
25	6026.0
26	7724.0
27	9749.0
28	11540.0
29	14316.0
30	17895.0
31	22572.0
32	28408.0
33	37533.0
34	55472.0
35	66362.0
36	67749.0
37	89651.0
38	121679.0
39	126542.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02583962314115	19.045915000636974	22.27858755226397	14.649657823957906
2	30.309454250326002	26.94251642888736	25.360820020125473	17.387209300661162
3	31.43138515094823	25.982619947696662	26.04502896436817	16.540965936986932
4	28.36131957853874	26.07465893329249	26.867224817031364	18.696796671137406
5	25.05321227510925	30.375584905606363	24.41122961508237	20.159973204202018
6	23.7565074998175	36.82003157667219	24.174762423473805	15.2486985000365
7	78.42566098448792	6.644126896425366	9.78447418256355	5.145737936523159
8	78.85880246257963	6.830638246707424	8.308558194260947	6.00200109645199
9	72.76919971887314	7.837770910241233	11.145878213670724	8.247151157214898
10	39.67309699019634	24.486091807098884	18.266804271868565	17.57400693083621
11	30.11048972469894	25.883137684883135	23.078882993113538	20.927489597304387
12	26.70247073861644	21.26501359113792	29.953465203394707	22.079050466850937
13	24.789691633613266	23.847544505787862	31.51383376012894	19.84893010046993
14	20.697034283448584	28.40726750136341	28.78487068021534	22.110827534972668
15	18.45932749990338	25.812999111100932	33.67925487069453	22.04841851830116
16	20.401593433884376	24.674750256578353	30.629085750847747	24.294570558689525
17	20.58180662652068	24.137689177331787	27.953943290815996	27.326560905331533
18	22.328972813429964	23.976656737525712	29.833370788286	23.860999660758328
19	22.80290917627255	26.821563174099683	26.465717267114886	23.909810382512877
20	24.159446449198917	25.703926471872286	27.519656693152328	22.61697038577647
21	23.780125590989055	27.240104377648983	27.753547365723996	21.226222665637966
22	21.233522802909178	25.735130980208037	26.149521125309004	26.88182509157378
23	20.741407666861814	27.903414889703516	25.527578057791324	25.827599385643353
24	22.026518106487533	25.417789718830203	28.063302209937635	24.492389964744632
25	21.81352586610403	27.637317729170636	24.93784147823486	25.61131492649048
26	20.536144983589004	27.820966280522804	26.683719405625688	24.959169330262505
27	22.0748994084026	31.007261489485654	24.75304780731073	22.164791294801013
28	20.083536472774068	26.75314227967542	29.545230075992997	23.61809117155752
29	21.959099191688722	26.60112765649848	27.18313467894426	24.256638472868538
30	25.16013781514048	26.09756132473158	26.96685021979139	21.77545064033655
31	24.910501748454447	26.73066930807581	25.42151135743905	22.937317586030687
32	26.195612188080165	27.057171526029283	25.60129513023588	21.145921155654673
33	26.679282067284365	24.900481952199847	24.842796553762646	23.57743942675314
34	22.610099668344745	25.491793071167752	28.879343044901567	23.018764215585936
35	25.017427288485678	24.64583598738651	26.949673426212073	23.387063297915738
36	27.651059164034088	25.37098295632657	26.215365500696375	20.76259237894297
37	24.558806899917982	25.536309594527474	29.818627373797085	20.08625613175746
38	25.39073626894278	25.81757958938875	26.776903510793463	22.014780630875
39	25.992353464058276	23.420701185341898	28.657476127835423	21.929469222764407
40	26.595545198585203	23.115526819416075	28.638724794844673	21.650203187154048
41	23.97465277827479	24.225147684639794	27.13088859847384	24.66931093861157
42	25.051065175911834	24.50097836153429	27.105695967890846	23.342260494663027
43	25.372843775630997	23.4882632400872	28.390233847730588	22.748659136551215
44	23.485686721050303	23.731171729288008	28.387657328693688	24.395484220967997
45	24.299294176923837	22.896808981172803	27.53153730871135	25.272359533192006
46	26.12175197568911	24.38689582417834	26.82199259393917	22.669359606193378
47	23.00831499949185	24.47678771057675	29.761943954985348	22.752953334946042
48	23.851266144396714	23.801167163123715	28.208159835789854	24.139406856689718
49	24.27567608575228	22.84127068193302	29.46907962445804	23.413973607856665
50	23.471945286186852	21.963536530030044	30.01587422006622	24.548643963716888
51	22.26713635654443	22.850431638508653	26.886691849754584	27.99574015519233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2642.0
1	2965.0
2	3288.0
3	3140.5
4	2993.0
5	2212.0
6	1431.0
7	1399.5
8	1368.0
9	1403.0
10	1438.0
11	1506.5
12	1575.0
13	1582.5
14	1590.0
15	1661.5
16	1733.0
17	1668.0
18	1603.0
19	1619.5
20	1636.0
21	1761.0
22	1886.0
23	2221.0
24	2556.0
25	3256.5
26	4741.0
27	5525.0
28	5950.0
29	6375.0
30	7671.5
31	8968.0
32	10049.5
33	11131.0
34	12496.0
35	13861.0
36	14622.0
37	15383.0
38	16643.0
39	17903.0
40	20996.0
41	24089.0
42	29020.5
43	33952.0
44	39468.0
45	44984.0
46	55996.0
47	67008.0
48	69656.5
49	72305.0
50	71496.0
51	70687.0
52	61735.0
53	52783.0
54	46310.0
55	39837.0
56	37557.0
57	35277.0
58	33363.5
59	31450.0
60	29418.5
61	27387.0
62	25164.5
63	22942.0
64	19961.5
65	16981.0
66	14976.0
67	12971.0
68	11467.0
69	9963.0
70	9389.5
71	8816.0
72	7865.5
73	6915.0
74	5602.0
75	3687.5
76	3086.0
77	2529.0
78	1972.0
79	1508.0
80	1044.0
81	796.0
82	548.0
83	414.5
84	281.0
85	204.0
86	127.0
87	85.0
88	43.0
89	27.5
90	12.0
91	8.0
92	4.0
93	5.0
94	6.0
95	10.0
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	698617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.162106616359615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17297653622254	24.498833926434486
2	10.172698713174281	6.543508411784309
3	3.803295285360525	3.6696596538378925
4	1.8720922486374203	2.408417219853485
5	1.1201008805725676	1.8012401971026608
6	0.7070495901216672	1.3644122580327855
7	0.5460046574620947	1.229246200442736
8	0.3833121332988279	0.9862500558800921
9	0.32875534450063765	0.9516117996474788
>10	3.952564814640416	30.617105257976906
>50	0.8457600222014066	17.724611882293033
>100	0.08879601064116587	4.922552915674991
>500	0.0026375052665692828	0.6062354788446885
>1k	0.003956257899853924	2.6763147421944855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3299	0.4722186834846561	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2389	0.341961332174854	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2364	0.3383828335124968	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	2230	0.31920208068226225	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	2178	0.31175880346455925	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	2045	0.29272119058081897	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1948	0.27883661577087304	Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1451	0.20769606236321186	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026	0.14686158510313949	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	847	0.12123953468066194	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	840	0.12023755505520192	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	813	0.11637277649985615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.43139946494288E-4	0.0	0.0	0.3043155262468563	0.0
2	1.43139946494288E-4	0.0	0.0	1.4481468386827117	0.0
3	1.43139946494288E-4	0.0	0.0	1.8729861998777584	0.0
4	1.43139946494288E-4	0.0	0.0	2.4127669381077186	0.0
5	1.43139946494288E-4	0.0	0.0	4.140466092293774	0.0
6	1.43139946494288E-4	0.0	0.0	5.256098835270255	0.0
7	1.43139946494288E-4	0.0	0.0	6.290857508477464	0.0
8	1.43139946494288E-4	0.0	0.0	7.95915358486839	0.0
9	1.43139946494288E-4	0.0	0.0	8.608579522112974	0.0
10	1.43139946494288E-4	0.0	0.0	10.323825500954028	0.0
11	1.43139946494288E-4	0.0	0.0	12.077719265348538	0.0
12	2.86279892988576E-4	0.0	0.0	13.695630080573476	0.0
13	2.86279892988576E-4	0.0	0.0	14.24986795339936	0.0
14	2.86279892988576E-4	0.0	0.0	14.479178147683209	0.0
15	2.86279892988576E-4	0.0	0.0	14.883691636476065	0.0
16	2.86279892988576E-4	0.0	0.0	15.646341271397633	0.0
17	2.86279892988576E-4	0.0	0.0	16.489864976088473	0.0
18	2.86279892988576E-4	0.0	0.0	17.51302931362964	0.0
19	2.86279892988576E-4	0.0	0.0	18.118654427246977	0.0
20	2.86279892988576E-4	0.0	0.0	18.602467446397668	0.0
21	2.86279892988576E-4	0.0	0.0	19.137667706339812	0.0
22	2.86279892988576E-4	0.0	0.0	19.717527629588172	0.0
23	4.29419839482864E-4	0.0	0.0	20.288656016100383	0.0
24	4.29419839482864E-4	0.0	0.0	20.747419544614573	0.0
25	4.29419839482864E-4	0.0	0.0	21.114000947586447	0.0
26	4.29419839482864E-4	0.0	0.0	21.481870610076765	0.0
27	4.29419839482864E-4	0.0	0.0	21.892825396461866	0.0
28	4.29419839482864E-4	0.0	0.0	22.315660798405993	0.0
29	4.29419839482864E-4	0.0	0.0	22.73978445986857	0.0
30	4.29419839482864E-4	0.0	0.0	23.225315158377192	0.0
31	4.29419839482864E-4	0.0	0.0	23.64815056032132	0.0
32	4.29419839482864E-4	0.0	0.0	24.072560501676886	0.0
33	4.29419839482864E-4	0.0	0.0	24.501837201213256	0.0
34	5.72559785977152E-4	0.0	0.0	24.924386323264393	0.0
35	5.72559785977152E-4	0.0	0.0	25.369408416915135	0.0
36	5.72559785977152E-4	0.0	0.0	25.782653442444143	0.0
37	5.72559785977152E-4	0.0	0.0	26.184304132307116	0.0
38	5.72559785977152E-4	0.0	0.0	26.568921168537265	0.0
39	5.72559785977152E-4	0.0	0.0	26.980448514708346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.0313836E-4	45.000004	19
TCGTTGC	40	6.8084773E-9	45.000004	16
CGACGGT	30	2.1642736E-6	45.000004	28
ACGCATC	35	1.2110104E-7	45.000004	10
CGCGATT	25	3.8892285E-5	45.000004	30
TTGCGTA	20	7.0313836E-4	45.000004	14
GGACGAT	20	7.0313836E-4	45.000004	8
TATAGCG	50	2.1827873E-11	45.000004	1
CGGTCGA	35	1.2110104E-7	45.000004	41
GCTACGA	30	2.1642736E-6	45.000004	10
AATCGTG	25	3.8892285E-5	45.000004	14
CGTAAGG	85	0.0	42.35294	2
ATTGCGG	80	0.0	42.187504	2
TCGTTTA	65	0.0	41.538464	38
ACGCATT	65	0.0	41.538464	17
TACGCAT	65	0.0	41.538464	16
TATTAGT	50	1.0804797E-9	40.500004	30
GTTGCGC	50	1.0804797E-9	40.500004	9
CGTTGCG	45	1.9264917E-8	40.0	17
GTTAGCG	90	0.0	40.0	1
>>END_MODULE