SRR3553963_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869739
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2148	0.24697064291701304	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1996	0.22949413559700096	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1185	0.13624777088298903	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1092	0.12555490785166584	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.12498002274245491	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	985	0.11325236651455207	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	935	0.10750351542244281	RNA PCR Primer, Index 38 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	20	7.0323376E-4	45.000004	17
CCGTAAG	20	7.0323376E-4	45.000004	41
CGTCGGA	20	7.0323376E-4	45.000004	18
TTACGAG	20	7.0323376E-4	45.000004	1
TATAACG	20	7.0323376E-4	45.000004	1
ATCGCGA	20	7.0323376E-4	45.000004	25
CGTTCGA	25	3.89002E-5	45.0	39
CCGTAAT	50	2.1827873E-11	45.0	18
GCGATAT	35	1.2114651E-7	45.0	9
AATCGAC	25	3.89002E-5	45.0	33
CCGTAGG	30	2.1648902E-6	44.999996	2
CGGTCTA	60	0.0	44.999996	31
GTGCGAC	30	2.1648902E-6	44.999996	22
CCAACGG	70	0.0	41.785713	2
CGACAGG	125	0.0	41.4	2
CGAATAT	240	0.0	41.249996	14
TTACGCG	55	6.002665E-11	40.909092	1
GCTACGA	245	0.0	40.408165	10
ACGGGCT	235	0.0	40.212765	5
CGATTGC	85	0.0	39.705883	10