##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869739
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777380340538944	31.0	31.0	34.0	30.0	34.0
2	31.88030547095163	33.0	31.0	34.0	30.0	34.0
3	31.910428300904066	33.0	31.0	34.0	30.0	34.0
4	35.55634391466865	37.0	35.0	37.0	33.0	37.0
5	35.39346171667592	37.0	35.0	37.0	33.0	37.0
6	35.342043992508096	37.0	35.0	37.0	33.0	37.0
7	35.69890277428056	37.0	35.0	37.0	35.0	37.0
8	35.55410531205339	37.0	35.0	37.0	35.0	37.0
9	37.30213891753733	39.0	37.0	39.0	34.0	39.0
10	36.66782793458727	39.0	35.0	39.0	32.0	39.0
11	36.59378158275069	39.0	35.0	39.0	32.0	39.0
12	36.48458905487738	38.0	35.0	39.0	32.0	39.0
13	36.34493911391808	39.0	35.0	39.0	32.0	39.0
14	37.334572785628794	39.0	36.0	41.0	32.0	41.0
15	37.50836170391347	39.0	36.0	41.0	32.0	41.0
16	37.567404704169874	39.0	36.0	41.0	32.0	41.0
17	37.525550768678876	39.0	36.0	41.0	32.0	41.0
18	37.531165096655435	39.0	36.0	41.0	32.0	41.0
19	37.460272564527976	39.0	36.0	41.0	32.0	41.0
20	37.372432419380985	39.0	35.0	41.0	32.0	41.0
21	37.26216485635346	39.0	35.0	41.0	32.0	41.0
22	37.25987106476771	39.0	35.0	41.0	32.0	41.0
23	36.74335404069497	39.0	35.0	41.0	31.0	41.0
24	36.84129031812992	39.0	35.0	41.0	31.0	41.0
25	36.8556566970091	39.0	35.0	41.0	31.0	41.0
26	36.88519199437992	39.0	35.0	40.0	31.0	41.0
27	34.40622531587062	38.0	35.0	40.0	23.0	41.0
28	35.2781512614704	38.0	34.0	40.0	22.0	41.0
29	35.96477563958843	38.0	34.0	40.0	27.0	41.0
30	36.264201099410286	38.0	35.0	40.0	30.0	41.0
31	36.337574835669095	38.0	35.0	40.0	30.0	41.0
32	36.12332780293858	38.0	35.0	40.0	30.0	41.0
33	36.17589414755461	38.0	35.0	40.0	30.0	41.0
34	36.114100896935746	38.0	35.0	40.0	30.0	41.0
35	36.05475780665234	38.0	35.0	40.0	29.0	41.0
36	35.96389146629046	38.0	35.0	40.0	29.0	41.0
37	35.840887898553476	38.0	35.0	40.0	29.0	41.0
38	35.77762984067634	38.0	35.0	40.0	29.0	41.0
39	35.671277245242536	38.0	34.0	40.0	28.0	41.0
40	35.519800767816555	38.0	34.0	40.0	27.0	41.0
41	35.396782253066725	38.0	34.0	40.0	27.0	41.0
42	35.316037339937616	38.0	34.0	40.0	27.0	41.0
43	35.20833376449716	38.0	34.0	40.0	26.0	41.0
44	35.065739262008485	38.0	34.0	40.0	26.0	41.0
45	35.0175006524946	37.0	34.0	40.0	26.0	41.0
46	34.865942541383106	37.0	34.0	40.0	26.0	41.0
47	34.73288883216689	37.0	33.0	40.0	26.0	41.0
48	34.63371310243648	37.0	33.0	40.0	25.0	41.0
49	34.56000478304411	37.0	33.0	40.0	25.0	41.0
50	34.42768462722725	36.0	33.0	40.0	24.0	41.0
51	32.79609744992463	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	5.0
11	6.0
12	6.0
13	18.0
14	41.0
15	71.0
16	160.0
17	265.0
18	501.0
19	887.0
20	1504.0
21	2171.0
22	3049.0
23	4536.0
24	5918.0
25	7689.0
26	9920.0
27	11940.0
28	14548.0
29	17562.0
30	22088.0
31	27061.0
32	34545.0
33	45451.0
34	66702.0
35	79726.0
36	82544.0
37	109930.0
38	150880.0
39	169971.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.777456225373356	18.722973213803222	23.200523375403424	13.299047185419994
2	30.49547048022453	24.846189489030618	26.2778833650095	18.380456665735352
3	32.79512589409006	24.476078455720625	26.480242923451748	16.24855272673756
4	28.173624501143447	26.603153359801045	25.156512470982673	20.066709668072836
5	23.910046577191547	32.061342540693246	24.38697126379293	19.64163961832228
6	24.576108464723326	37.16885180496678	24.13643633319881	14.118603397111087
7	82.47784680231656	6.1055098138637	6.931389761756113	4.485253622063631
8	83.12436259613516	5.433469121196128	6.970366972160614	4.471801310508095
9	77.03943366918121	7.76163883647853	9.535159398394232	5.663768095946025
10	40.92043705065543	26.307087528557417	16.441484169388747	16.33099125139841
11	31.62121050108136	26.806202780374345	21.681331985802636	19.89125473274166
12	28.258822474328504	23.283076877086113	28.426114041108885	20.031986607476497
13	25.38209738783704	24.6728041400926	29.336272146011623	20.60882632605874
14	21.522893649704105	28.68009828235827	28.43899146755521	21.358016600382413
15	21.257641660314185	26.525198938992045	32.1435511113104	20.073608289383365
16	23.689520649298238	23.504177690088635	30.554913600516937	22.25138806009619
17	23.313200856808766	23.935111567953143	27.652088730067298	25.099598845170796
18	25.42762828848655	24.356732307048436	27.251393808947284	22.964245595517735
19	25.502133398640282	26.195329863326815	25.655167814712232	22.647368923320673
20	25.65459292960302	26.074833944436204	25.909726941070826	22.360846184889947
21	25.428318150617603	26.575903805624446	27.302673560688895	20.693104483069057
22	23.585236490487375	24.519424792955128	26.045974711953818	25.849364004603682
23	22.626328128323557	27.938726445519862	25.789231022180214	23.645714403976363
24	22.069379434520012	26.314446057955315	28.091875838613657	23.52429866891102
25	23.841175341108077	26.16083675677416	25.89995389421424	24.09803400790352
26	21.81769473370747	26.71330134672586	26.034936917856967	25.43406700170971
27	22.01361557892655	31.239946696652677	24.817100302504542	21.92933742191623
28	21.95520725183072	27.541825766120642	26.623849223732638	23.879117758316003
29	22.902617911810324	26.72859329063087	25.81912504785919	24.549663749699622
30	24.348453961475798	25.911221642354775	25.95985692259402	23.780467473575406
31	24.838715982610875	27.634727199769127	23.613750791904238	23.91280602571576
32	24.369149825407394	26.421489665290395	24.454462775614296	24.754897733687923
33	25.058207117307607	25.880062869435545	24.111946227546426	24.949783785710427
34	22.437420881436847	26.909682100032306	25.781412584694944	24.871484433835896
35	22.771084198822866	25.390720664475204	25.684257001238304	26.153938135463626
36	25.691155622548834	27.26841040817993	24.338795891641055	22.701638077630186
37	23.692050143778765	28.31838057164276	25.64378508954985	22.345784195028624
38	22.37970241647207	28.2522687840835	26.089551003232003	23.278477796212428
39	24.193694890076216	24.844119902637456	26.242585419303953	24.71959978798237
40	23.682392073944023	24.91851003576935	28.34735478114699	23.05174310913964
41	21.76204585513585	25.705642727300948	27.603568426849893	24.928742990713307
42	23.638930759687675	24.94886396953569	26.747564499234826	24.66464077154181
43	23.635136517966885	23.881187344709158	27.189421194174347	25.294254943149614
44	22.77614318778392	24.838486028567193	26.767685478057214	25.617685305591674
45	23.474858549518878	24.29751914079971	26.497489476728077	25.730132832953334
46	23.60006852630502	25.4945449151987	26.520829812162038	24.384556746334248
47	22.064550399602638	25.302648265744093	28.40012923417255	24.232672100480716
48	22.639435508813563	25.82303426660182	27.678418468069154	23.85911175651546
49	23.860491480777565	24.430892486136646	27.800409088243715	23.90820694484207
50	22.33244686049493	24.448254016434813	28.258247589219295	24.96105153385096
51	22.419024557942098	24.00478764318951	26.65351329536792	26.922674503500478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2487.0
1	2771.5
2	3056.0
3	3035.5
4	3015.0
5	2274.5
6	1534.0
7	1506.0
8	1478.0
9	1645.5
10	1813.0
11	1872.5
12	1932.0
13	1945.0
14	1958.0
15	2019.5
16	2081.0
17	2104.0
18	2127.0
19	2135.0
20	2143.0
21	2399.0
22	2655.0
23	3011.5
24	3368.0
25	4030.0
26	5475.5
27	6259.0
28	8211.0
29	10163.0
30	11338.0
31	12513.0
32	13208.0
33	13903.0
34	16551.0
35	19199.0
36	20606.5
37	22014.0
38	24350.0
39	26686.0
40	30680.0
41	34674.0
42	38726.0
43	42778.0
44	47512.0
45	52246.0
46	58397.0
47	64548.0
48	71568.5
49	78589.0
50	77605.5
51	76622.0
52	70565.0
53	64508.0
54	58755.5
55	53003.0
56	51088.5
57	49174.0
58	45100.5
59	41027.0
60	40039.5
61	39052.0
62	34915.5
63	30779.0
64	27064.5
65	23350.0
66	21123.5
67	18897.0
68	17050.5
69	15204.0
70	14048.0
71	12892.0
72	11695.5
73	10499.0
74	8640.5
75	5706.0
76	4630.0
77	3496.0
78	2362.0
79	1956.0
80	1550.0
81	1115.0
82	680.0
83	512.5
84	345.0
85	285.0
86	225.0
87	152.5
88	80.0
89	60.5
90	41.0
91	46.0
92	51.0
93	60.5
94	70.0
95	37.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	869739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.3605746908602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22944691838838	21.335503482810562
2	10.037294332209909	5.693268712055737
3	3.845625269955559	3.271924281049022
4	2.014572408685881	2.2853772506672825
5	1.2107497560548448	1.7168779444267097
6	0.8344296782656138	1.4198943128833428
7	0.576216834012662	1.1439288391402966
8	0.4053216799179963	0.9196124621711413
9	0.3416144056739693	0.8719542780831379
>10	4.004510489877975	27.14089937608128
>50	1.3132510356552667	25.61025183966074
>100	0.1816718242280688	7.0303716676073575
>500	0.0032586874300998886	0.6927902934854056
>1k	0.0020366796438124305	0.8673452598780098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2148	0.24697064291701304	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1996	0.22949413559700096	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1185	0.13624777088298903	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1092	0.12555490785166584	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.12498002274245491	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	985	0.11325236651455207	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	935	0.10750351542244281	RNA PCR Primer, Index 38 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5990808736873933E-4	0.0	0.0	0.134983023642725	0.0
2	4.5990808736873933E-4	0.0	0.0	0.6722706467112548	0.0
3	4.5990808736873933E-4	0.0	0.0	0.9106180129901039	0.0
4	4.5990808736873933E-4	0.0	0.0	1.2157670289592626	0.0
5	4.5990808736873933E-4	0.0	0.0	2.1040794997119825	0.0
6	4.5990808736873933E-4	0.0	0.0	2.779914434100345	0.0
7	4.5990808736873933E-4	0.0	0.0	3.388832741776556	0.0
8	4.5990808736873933E-4	0.0	0.0	4.440182629501495	0.0
9	4.5990808736873933E-4	0.0	0.0	4.921821373998406	0.0
10	4.5990808736873933E-4	0.0	0.0	5.954200053119384	0.0
11	4.5990808736873933E-4	0.0	0.0	7.136048860635202	0.0
12	4.5990808736873933E-4	0.0	0.0	8.063453518814265	0.0
13	4.5990808736873933E-4	0.0	0.0	8.41781270013188	0.0
14	4.5990808736873933E-4	0.0	0.0	8.566133058308298	0.0
15	4.5990808736873933E-4	0.0	0.0	8.827015920868215	0.0
16	4.5990808736873933E-4	0.0	0.0	9.38821876447992	0.0
17	4.5990808736873933E-4	0.0	0.0	10.027031097835097	0.0
18	4.5990808736873933E-4	0.0	0.0	10.861649299387517	0.0
19	4.5990808736873933E-4	0.0	0.0	11.242338218706992	0.0
20	4.5990808736873933E-4	0.0	0.0	11.612564229038826	0.0
21	4.5990808736873933E-4	0.0	0.0	12.033495106003066	0.0
22	4.5990808736873933E-4	0.0	0.0	12.473627145614948	0.0
23	4.5990808736873933E-4	0.0	0.0	12.911804575855514	0.0
24	5.748851092109242E-4	0.0	0.0	13.24201858258627	0.0
25	8.048391528952939E-4	0.0	0.0	13.54038395426674	0.0
26	9.198161747374787E-4	0.0	0.0	13.83667973955405	0.0
27	9.198161747374787E-4	0.0	0.0	14.154131296860323	0.0
28	9.198161747374787E-4	0.0	0.0	14.499062362386876	0.0
29	0.0010347931965796636	0.0	0.0	14.847787669634224	0.0
30	0.001494701283948403	0.0	0.0	15.242848716683971	0.0
31	0.001494701283948403	0.0	0.0	15.589389460516315	0.0
32	0.0016096783057905878	0.0	0.0	15.947197952489194	0.0
33	0.0016096783057905878	0.0	0.0	16.31018041044497	0.0
34	0.0016096783057905878	0.0	0.0	16.657525993430212	0.0
35	0.0016096783057905878	0.0	0.0	17.060865386052598	0.0
36	0.0016096783057905878	0.0	0.0	17.402577094967572	0.0
37	0.0016096783057905878	0.0	0.0	17.755441575001235	0.0
38	0.0016096783057905878	0.0	0.0	18.127852148748072	0.0
39	0.0016096783057905878	0.0	0.0	18.559015980656266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	20	7.0323376E-4	45.000004	17
CCGTAAG	20	7.0323376E-4	45.000004	41
CGTCGGA	20	7.0323376E-4	45.000004	18
TTACGAG	20	7.0323376E-4	45.000004	1
TATAACG	20	7.0323376E-4	45.000004	1
ATCGCGA	20	7.0323376E-4	45.000004	25
CGTTCGA	25	3.89002E-5	45.0	39
CCGTAAT	50	2.1827873E-11	45.0	18
GCGATAT	35	1.2114651E-7	45.0	9
AATCGAC	25	3.89002E-5	45.0	33
CCGTAGG	30	2.1648902E-6	44.999996	2
CGGTCTA	60	0.0	44.999996	31
GTGCGAC	30	2.1648902E-6	44.999996	22
CCAACGG	70	0.0	41.785713	2
CGACAGG	125	0.0	41.4	2
CGAATAT	240	0.0	41.249996	14
TTACGCG	55	6.002665E-11	40.909092	1
GCTACGA	245	0.0	40.408165	10
ACGGGCT	235	0.0	40.212765	5
CGATTGC	85	0.0	39.705883	10
>>END_MODULE