##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863967
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78752197711255	31.0	31.0	34.0	30.0	34.0
2	31.90700686484553	33.0	31.0	34.0	30.0	34.0
3	31.92750186060347	33.0	31.0	34.0	30.0	34.0
4	35.581033766335985	37.0	35.0	37.0	33.0	37.0
5	35.418307643694725	37.0	35.0	37.0	33.0	37.0
6	35.3607325279785	37.0	35.0	37.0	33.0	37.0
7	35.673140293552876	37.0	35.0	37.0	35.0	37.0
8	35.52104652145279	37.0	35.0	37.0	33.0	37.0
9	37.300305451481364	39.0	37.0	39.0	34.0	39.0
10	36.70150711774871	39.0	37.0	39.0	32.0	39.0
11	36.616936758001174	39.0	35.0	39.0	32.0	39.0
12	36.48795960956842	38.0	35.0	39.0	32.0	39.0
13	36.35503439367476	39.0	35.0	39.0	32.0	39.0
14	37.43515898176666	39.0	36.0	41.0	32.0	41.0
15	37.5957878020804	39.0	36.0	41.0	33.0	41.0
16	37.636726865725194	39.0	36.0	41.0	33.0	41.0
17	37.576960694100585	39.0	36.0	41.0	32.0	41.0
18	37.58986743706646	39.0	36.0	41.0	33.0	41.0
19	37.513614524628835	39.0	36.0	41.0	32.0	41.0
20	37.37525854575464	39.0	35.0	41.0	32.0	41.0
21	37.293456810271685	39.0	35.0	41.0	32.0	41.0
22	37.28837212532423	39.0	35.0	41.0	32.0	41.0
23	36.74779476530932	39.0	35.0	41.0	31.0	41.0
24	36.87150203653612	39.0	35.0	41.0	31.0	41.0
25	36.867026171138484	39.0	35.0	41.0	31.0	41.0
26	36.886585945990994	39.0	35.0	41.0	31.0	41.0
27	34.44345096514103	38.0	35.0	40.0	23.0	41.0
28	35.33047905764919	38.0	35.0	40.0	22.0	41.0
29	36.0054064564966	38.0	35.0	40.0	27.0	41.0
30	36.32208058872619	38.0	35.0	40.0	30.0	41.0
31	36.39267240531178	38.0	35.0	40.0	30.0	41.0
32	36.16374236515978	38.0	35.0	40.0	30.0	41.0
33	36.18162383516963	38.0	35.0	40.0	30.0	41.0
34	36.14706464483019	38.0	35.0	40.0	30.0	41.0
35	36.081918638096134	38.0	35.0	40.0	30.0	41.0
36	35.99380994875962	38.0	35.0	40.0	29.0	41.0
37	35.90309236348148	38.0	35.0	40.0	29.0	41.0
38	35.82229298109766	38.0	35.0	40.0	29.0	41.0
39	35.68620213503525	38.0	35.0	40.0	28.0	41.0
40	35.56260482171194	38.0	34.0	40.0	28.0	41.0
41	35.46153151682877	38.0	34.0	40.0	27.0	41.0
42	35.37139612971329	38.0	34.0	40.0	27.0	41.0
43	35.24126384456814	38.0	34.0	40.0	27.0	41.0
44	35.130908935179235	38.0	34.0	40.0	26.0	41.0
45	35.06152318317714	37.0	34.0	40.0	26.0	41.0
46	34.9025240547382	37.0	34.0	40.0	26.0	41.0
47	34.74640466591895	37.0	33.0	40.0	26.0	41.0
48	34.656174367770994	37.0	33.0	40.0	25.0	41.0
49	34.61416813373659	37.0	33.0	40.0	26.0	41.0
50	34.457746650045664	36.0	33.0	40.0	24.0	41.0
51	32.945406479645634	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	8.0
12	12.0
13	17.0
14	35.0
15	73.0
16	129.0
17	237.0
18	504.0
19	855.0
20	1496.0
21	2265.0
22	3421.0
23	4540.0
24	6006.0
25	7690.0
26	9179.0
27	11292.0
28	13671.0
29	17067.0
30	20904.0
31	26472.0
32	33784.0
33	44543.0
34	66286.0
35	80952.0
36	81560.0
37	109555.0
38	150514.0
39	170867.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08378213519729	18.583117179244116	23.466637035905308	12.866463649653284
2	30.769346514392332	25.25351084011311	26.17426360034585	17.802879045148714
3	32.76757098361396	25.331060098360236	25.696699063737388	16.20466985428842
4	27.826641526817575	26.47473803976309	26.151230313194834	19.5473901202245
5	24.91889157803481	31.73581861344241	24.198262202144292	19.147027606378483
6	24.21921207638718	37.72887158884541	23.807043556061746	14.24487277870567
7	81.16953541049601	6.703149541591287	7.690687260045811	4.436627787866898
8	83.01786989549369	6.316792192294382	6.67791709637058	3.987420815841346
9	77.50481210509199	7.960489231648895	8.46999943284871	6.0646992304104215
10	41.915258337413356	26.167781871298324	15.630805343259638	16.286154448028686
11	31.92726111066742	26.995244031311383	21.07221687865393	20.005277979367268
12	27.423038148447798	23.701252478393272	28.368907608739686	20.506801764419244
13	24.803377906795053	25.291128017621045	29.727871550649503	20.177622524934403
14	21.850950325649013	29.1191677459903	27.55799700683012	21.471884921530567
15	21.066892601222037	25.453286988970643	32.18051152416701	21.299308885640308
16	23.034791838114188	23.942812630575013	30.490516420187348	22.531879111123455
17	22.796588295617774	23.918853382131495	27.708581462023435	25.5759768602273
18	24.03228364046312	24.177891053709228	27.73763349757572	24.052191808251937
19	25.115311117207025	26.6243965336639	25.976455119234878	22.283837229894196
20	25.969973390187356	26.06233802911454	26.142317935754487	21.825370644943614
21	24.92722522966734	27.514013845436224	26.245446874707017	21.313314050189415
22	23.52173173280924	24.536932544877292	26.601594736836017	25.339740985477455
23	22.437431059288144	27.86587913658739	26.068125287192682	23.628564516931778
24	22.517295220766535	26.380521478250905	27.914607849605368	23.187575451377192
25	23.166278341649623	26.715372230652328	26.34047365235015	23.7778757753479
26	21.148724430446997	27.590752887552416	27.03251397333463	24.228008708665957
27	21.516215318409152	31.461271090215252	25.069707523551248	21.952806067824348
28	22.07352827133444	27.422112187155296	28.102346501660364	22.4020130398499
29	23.350891874342423	26.437120862255153	26.942811473123395	23.269175790279025
30	23.136531835127965	26.471497175239335	27.176385209157296	23.21558578047541
31	24.848402774643013	26.62914208528798	24.13784322780847	24.38461191226054
32	23.74303648171747	27.414704496815272	25.057554281587148	23.784704739880112
33	23.145096977083615	26.315472697452563	24.363546292856093	26.175884032607726
34	21.772938086755627	26.501359426922555	26.011294412865304	25.714408073456507
35	22.225848903951192	25.54125331175843	26.833432295446467	25.3994654888439
36	24.46169818986142	25.66290147656103	25.038340584767703	24.83705974880985
37	22.33048253000404	27.374077945106702	25.692532237921125	24.602907286968136
38	22.673782679199554	28.10882823070789	23.891074543356403	25.32631454673616
39	23.735050065569634	25.601903776417384	24.264236944235137	26.39880921377784
40	23.66305657507752	24.448271751120124	26.540018310884562	25.348653362917794
41	22.125150613391483	24.22453635381907	26.364432900793666	27.285880131995782
42	22.315782894485555	25.579796450558877	26.34058939751171	25.763831257443858
43	23.26373576768557	23.602753345903256	27.127077770331503	26.006433116079663
44	21.71946382211358	25.195869749654793	27.057167692747523	26.027498735484112
45	23.734587084923383	24.246064953869766	26.490132146250957	25.529215814955897
46	25.09343528167164	24.653835158055806	25.374233043623196	24.878496516649363
47	22.525744617560626	25.297609746668563	27.327432644996858	24.849212990773953
48	22.75584599874764	26.174032110022722	26.126113613135686	24.944008278093953
49	23.636319442756495	24.60661113213815	26.632730185296428	25.12433923980893
50	22.02306338089302	24.418409499436898	27.989263478813424	25.569263640856654
51	22.522388007875303	23.998023072640507	25.626557495830284	27.853031423653913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2556.0
1	2939.5
2	3323.0
3	3286.5
4	3250.0
5	2500.5
6	1751.0
7	1715.5
8	1680.0
9	1749.5
10	1819.0
11	1855.5
12	1892.0
13	1955.0
14	2018.0
15	2063.5
16	2109.0
17	2181.5
18	2254.0
19	2190.5
20	2127.0
21	2677.5
22	3228.0
23	3121.5
24	3015.0
25	4311.5
26	6658.5
27	7709.0
28	8260.0
29	8811.0
30	9607.0
31	10403.0
32	13463.5
33	16524.0
34	17635.0
35	18746.0
36	20636.5
37	22527.0
38	23916.0
39	25305.0
40	28950.5
41	32596.0
42	36236.0
43	39876.0
44	45270.5
45	50665.0
46	54293.0
47	57921.0
48	65805.0
49	73689.0
50	75577.0
51	77465.0
52	73925.5
53	70386.0
54	64489.0
55	58592.0
56	53294.5
57	47997.0
58	44188.0
59	40379.0
60	38649.0
61	36919.0
62	33151.0
63	29383.0
64	26893.0
65	24403.0
66	21886.5
67	19370.0
68	17637.5
69	15905.0
70	14905.0
71	13905.0
72	12191.0
73	10477.0
74	8947.0
75	5915.5
76	4414.0
77	3682.5
78	2951.0
79	2008.0
80	1065.0
81	822.5
82	580.0
83	565.5
84	551.0
85	371.5
86	192.0
87	137.5
88	83.0
89	64.5
90	46.0
91	36.0
92	26.0
93	30.0
94	34.0
95	29.5
96	25.0
97	12.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	863967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.733244790788774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51790817104231	20.94356633395824
2	9.918713408910367	5.5015621395798044
3	3.7471122926157494	3.1175874740905893
4	1.90948831606459	2.118252275782813
5	1.1807598329577884	1.63731507432746
6	0.7883316171451545	1.3117796228762968
7	0.5786524131136973	1.123353631516396
8	0.43629270749513777	0.9679849965918929
9	0.3435744193961746	0.8575590129270514
>10	4.043201190144158	27.272179287961716
>50	1.334315345834376	25.284323346680875
>100	0.19413536781665006	7.663810827110794
>500	0.003757458731935162	0.7208775677533131
>1k	0.003757458731935162	1.4798484088427715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2289	0.26494067481744094	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1513	0.17512242944464315	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	1505	0.17419646815214007	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1436	0.16621005200430108	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	1306	0.15116318100112622	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1266	0.14653337453861084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC	1257	0.1454916680845449	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1119	0.12951883578886692	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1090	0.1261622261035433	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	983	0.11377749381631475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.787258078144189E-4	0.0	0.0	0.18287735526935636	0.0
2	5.787258078144189E-4	0.0	0.0	0.6391447821502442	0.0
3	5.787258078144189E-4	0.0	0.0	0.819475743865217	0.0
4	5.787258078144189E-4	0.0	0.0	1.0667074089635369	0.0
5	5.787258078144189E-4	0.0	0.0	1.847061288220499	0.0
6	5.787258078144189E-4	0.0	0.0	2.376942637855381	0.0
7	5.787258078144189E-4	0.0	0.0	2.8545071744638393	0.0
8	6.944709693773026E-4	0.0	0.0	3.7059285829204125	0.0
9	6.944709693773026E-4	0.0	0.0	4.072377764428502	0.0
10	6.944709693773026E-4	0.0	0.0	5.149039257286447	0.0
11	6.944709693773026E-4	0.0	0.0	6.281605663179265	0.0
12	6.944709693773026E-4	0.0	0.0	7.3338449269474415	0.0
13	6.944709693773026E-4	0.0	0.0	7.707701798795556	0.0
14	8.102161309401864E-4	0.0	0.0	7.847984934609771	0.0
15	8.102161309401864E-4	0.0	0.0	8.123226928806309	0.0
16	8.102161309401864E-4	0.0	0.0	8.6823918043166	0.0
17	8.102161309401864E-4	0.0	0.0	9.287044528321104	0.0
18	8.102161309401864E-4	0.0	0.0	10.05304600754427	0.0
19	8.102161309401864E-4	0.0	0.0	10.436162492317415	0.0
20	8.102161309401864E-4	0.0	0.0	10.767656635033514	0.0
21	8.102161309401864E-4	0.0	0.0	11.148110981090714	0.0
22	9.259612925030702E-4	0.0	0.0	11.546853062674847	0.0
23	9.259612925030702E-4	0.0	0.0	11.953465815245258	0.0
24	0.0011574516156288377	0.0	0.0	12.28148760311447	0.0
25	0.0011574516156288377	0.0	0.0	12.570156036052303	0.0
26	0.0013889419387546052	0.0	0.0	12.836369907646935	0.0
27	0.0013889419387546052	0.0	0.0	13.11670468895224	0.0
28	0.0013889419387546052	0.0	0.0	13.430374076787656	0.0
29	0.0013889419387546052	0.0	0.0	13.740918345260873	0.0
30	0.001504687100317489	0.0	0.0	14.079472942832307	0.0
31	0.0016204322618803728	0.0	0.0	14.403212159723694	0.0
32	0.0016204322618803728	0.0	0.0	14.723594766929756	0.0
33	0.0016204322618803728	0.0	0.0	15.053121241899285	0.0
34	0.0016204322618803728	0.0	0.0	15.361003371656556	0.0
35	0.0017361774234432566	0.0	0.0	15.713678878938664	0.0
36	0.0017361774234432566	0.0	0.0	16.014732044163726	0.0
37	0.0017361774234432566	0.0	0.0	16.34217510622512	0.0
38	0.0018519225850061403	0.0	0.0	16.67494244571841	0.0
39	0.0018519225850061403	0.0	0.0	17.01916855620643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAT	20	7.032314E-4	45.0	21
CAACGAC	25	3.889998E-5	45.0	12
CGTGCAT	20	7.032314E-4	45.0	19
TTCGTAA	20	7.032314E-4	45.0	18
GGATACG	40	6.8121153E-9	45.0	1
GTAGCGT	20	7.032314E-4	45.0	17
TCGTGAC	20	7.032314E-4	45.0	13
TCGTAAA	20	7.032314E-4	45.0	19
CGACTTT	20	7.032314E-4	45.0	45
ATACGCG	45	3.8380676E-10	45.0	1
GCGCTAA	20	7.032314E-4	45.0	9
TGTCGTG	20	7.032314E-4	45.0	22
GCGTATG	25	3.889998E-5	45.0	1
TTGTGCG	75	0.0	42.000004	1
TCGAGAG	75	0.0	42.000004	1
GCGTAAG	60	3.6379788E-12	41.249996	1
AGTACGG	105	0.0	40.714287	2
GGTATTC	50	1.0804797E-9	40.5	8
ACCCAAT	50	1.0804797E-9	40.5	42
TATGGTC	85	0.0	39.705883	43
>>END_MODULE