FastQCFastQC Report
Sat 18 Jun 2016
SRR3553961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346014
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC32400.2407107206908695No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.15259870996884134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19300.14338632436215373No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC13750.10215346942899553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC253.8911618E-545.00000411
AGCCGAT207.033717E-445.030
CGAAACG207.033717E-445.03
ACGTATG406.8157533E-945.01
ATCGTAG302.1657797E-644.9999961
TACGCAT600.044.99999616
CGTTTTA302.1657797E-644.99999640
GCTAACG650.041.538461
CGTATGG1150.041.086962
TCGTACA451.9286745E-840.00000434
CAATGCG451.9286745E-840.0000041
TCGTGTA4900.039.9489817
CGTGTAC4950.039.54545218
ATAGCGT403.4588084E-739.37513
CGGTCTA800.039.37531
CGGGATA1550.039.1935466
GGTATAC750.039.08
TCGCTAA356.249087E-638.57142637
AACGCAC356.249087E-638.57142618
CGAATCC356.249087E-638.57142645
Produced by FastQC (version 0.11.5)