##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346014
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88391279734089	31.0	31.0	34.0	30.0	34.0
2	32.01428736996792	33.0	31.0	34.0	30.0	34.0
3	32.01344785418279	33.0	31.0	34.0	30.0	34.0
4	35.644210981460816	37.0	35.0	37.0	33.0	37.0
5	35.486773540245494	37.0	35.0	37.0	33.0	37.0
6	35.4274450340041	37.0	35.0	37.0	33.0	37.0
7	35.722343898354694	37.0	35.0	37.0	35.0	37.0
8	35.60681835404387	37.0	35.0	37.0	35.0	37.0
9	37.36395609555324	39.0	37.0	39.0	34.0	39.0
10	36.76648756996584	39.0	37.0	39.0	32.0	39.0
11	36.67272331491351	39.0	35.0	39.0	32.0	39.0
12	36.56718280790542	39.0	35.0	39.0	32.0	39.0
13	36.4911278783133	39.0	35.0	39.0	32.0	39.0
14	37.57690781819505	39.0	36.0	41.0	32.0	41.0
15	37.696544761050035	40.0	36.0	41.0	33.0	41.0
16	37.73235642422738	40.0	36.0	41.0	33.0	41.0
17	37.66585637296492	40.0	36.0	41.0	33.0	41.0
18	37.655597935831274	39.0	36.0	41.0	33.0	41.0
19	37.59294777023121	39.0	36.0	41.0	32.0	41.0
20	37.50674212898231	39.0	36.0	41.0	32.0	41.0
21	37.391765613136265	39.0	35.0	41.0	32.0	41.0
22	37.36037218037851	39.0	35.0	41.0	32.0	41.0
23	36.88896549367243	39.0	35.0	41.0	31.0	41.0
24	36.97322316112611	39.0	35.0	41.0	31.0	41.0
25	36.966188316020485	39.0	35.0	41.0	31.0	41.0
26	36.97620901417073	39.0	35.0	41.0	31.0	41.0
27	34.58999832096843	39.0	35.0	41.0	23.0	41.0
28	35.41230700423621	39.0	35.0	40.0	23.0	41.0
29	36.10316311717411	38.0	35.0	40.0	28.0	41.0
30	36.3838006142581	38.0	35.0	40.0	30.0	41.0
31	36.50181350268274	39.0	35.0	40.0	30.0	41.0
32	36.29224287414544	39.0	35.0	40.0	30.0	41.0
33	36.32648768883533	39.0	35.0	40.0	30.0	41.0
34	36.25746983315181	39.0	35.0	40.0	30.0	41.0
35	36.197133907968265	38.0	35.0	40.0	30.0	41.0
36	36.09770700750512	38.0	35.0	40.0	29.0	41.0
37	36.033408270642056	38.0	35.0	40.0	29.0	41.0
38	35.92621993530528	38.0	35.0	40.0	29.0	41.0
39	35.83513172968483	38.0	35.0	40.0	29.0	41.0
40	35.713402683775946	38.0	34.0	40.0	28.0	41.0
41	35.5952256068659	38.0	34.0	40.0	28.0	41.0
42	35.51897305674384	38.0	34.0	40.0	28.0	41.0
43	35.40295346110813	38.0	34.0	40.0	27.0	41.0
44	35.289505904099066	38.0	34.0	40.0	27.0	41.0
45	35.209719958336244	37.0	34.0	40.0	27.0	41.0
46	35.06037827243996	37.0	34.0	40.0	26.0	41.0
47	34.94371975328637	37.0	34.0	40.0	26.0	41.0
48	34.858671603712885	37.0	34.0	40.0	26.0	41.0
49	34.78721766638385	37.0	34.0	40.0	26.0	41.0
50	34.694487575909314	37.0	33.0	40.0	26.0	41.0
51	33.16337348645705	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	5.0
12	21.0
13	23.0
14	37.0
15	90.0
16	194.0
17	442.0
18	709.0
19	1344.0
20	2201.0
21	3361.0
22	4649.0
23	6282.0
24	8496.0
25	11083.0
26	14257.0
27	17643.0
28	21261.0
29	25742.0
30	31756.0
31	39423.0
32	50530.0
33	67443.0
34	101020.0
35	118309.0
36	127152.0
37	171307.0
38	235143.0
39	286004.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.563970359892245	18.675511547428183	24.783471791526683	13.977046301152885
2	32.04758642926448	24.415347834420743	25.35055356036416	18.18651217595062
3	33.25894084311159	24.422554297355006	25.703447363846145	16.615057495687267
4	28.889372621681495	26.570971772953328	24.778494131561782	19.761161473803394
5	25.407759503244392	31.240759754356194	23.900568641930917	19.450912100468496
6	25.09327540426771	36.62881663935145	23.27226908486836	15.005638871512481
7	82.23332001004448	6.444806666201094	7.244055411013556	4.077817912740878
8	84.44518407683724	5.174166093369014	6.364792639601075	4.015857190192673
9	78.30082005090586	7.285956906837522	8.745079917445137	5.66814312481148
10	41.87036687582744	25.399810105987008	16.21119839763925	16.518624620546294
11	31.831169660939633	26.479590851209572	21.551558899090203	20.137680588760592
12	27.314277563234857	23.154142527492287	28.58246645280064	20.949113456472222
13	25.189188225382498	24.718316451389065	29.512917399076088	20.579577924152346
14	22.242859286753333	27.22170794657411	28.237596340008352	22.29783642666421
15	21.6735487149465	25.681827975043348	31.25093795458294	21.39368535542721
16	24.59060604124474	23.157337145081698	29.322206158331195	22.929850655342364
17	23.560081841645033	23.763943019909153	27.488792835735737	25.187182302710077
18	24.80932590597126	24.480948935152234	27.228988702940683	23.480736455935823
19	25.59185862851352	26.57810394245528	25.450106759662233	22.379930669368967
20	26.76413469696452	25.155830474274417	25.63465164552523	22.445383183235837
21	26.382786508907042	25.689554492003797	26.05946149148523	21.86819750760393
22	24.72262547046316	24.331842016502055	25.879894265587133	25.065638247447648
23	24.07939293350589	27.100461065040925	24.876041408187433	23.94410459326575
24	23.884892727713083	25.944678138563194	26.59712306112715	23.57330607259657
25	24.90687318259691	25.881603014530306	25.579971679343604	23.631552123529175
26	22.765662169932853	26.422162028032396	25.713105510046702	25.099070291988046
27	22.20140355152324	30.42152607625181	25.10204202928053	22.275028342944427
28	22.89879600063595	26.590882412812945	27.3077397411914	23.202581845359706
29	23.795146261480195	25.761321947617187	25.629896865857265	24.813634925045356
30	24.13630170265688	25.66466619217928	26.190069345489718	24.00896275967412
31	25.58844113062717	26.785456912038065	23.794923381183256	23.83117857615151
32	24.53429161955225	26.631520920287606	24.382807311068085	24.45138014909206
33	24.07998728096439	26.66584448601575	23.853763779574358	25.400404453445507
34	22.182830193445238	27.310860065348503	25.574771139081765	24.931538602124494
35	23.47843335953415	24.76831593133504	25.535469913388713	26.217780795742097
36	23.370113535223258	28.5178311666892	24.544098352617432	23.567956945470105
37	22.681636297987986	28.351636758607267	25.692600522728593	23.274126420676158
38	22.337360532654195	28.980902130289877	25.060883467779682	23.62085386927625
39	23.552949672143082	26.28575928630757	25.223586084542955	24.93770495700639
40	23.288762226841623	25.61563252685336	28.050748357743675	23.044856888561338
41	21.346286145612154	26.421419093709275	27.11279377480472	25.11950098587385
42	22.089146175299813	27.212272680670484	25.917635329201627	24.78094581482808
43	22.93519978246883	24.60880793216118	27.19993997090669	25.256052314463297
44	23.21580607631124	25.292604683160796	26.200173252284152	25.29141598824381
45	24.25115934901123	24.639491119706037	25.68368531085115	25.42566422043159
46	24.228276971859135	25.81904794452361	26.069713985144283	23.882961098472975
47	22.9895082814889	25.920829946791045	27.73700719309012	23.352654578629938
48	21.812403139937622	26.264511364666344	27.024978937811937	24.898106557584097
49	22.87264471246213	26.185463152686378	26.8922165742704	24.049675560581093
50	21.851035724739862	25.128564784615907	28.168652034822816	24.85174745582141
51	22.192042579051925	25.244239658725686	26.48917470397782	26.074543058244565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2320.0
1	3018.0
2	3716.0
3	4447.5
4	5179.0
5	3952.0
6	2725.0
7	2608.0
8	2491.0
9	2622.5
10	2754.0
11	2813.5
12	2873.0
13	2777.0
14	2681.0
15	2737.0
16	2793.0
17	3249.0
18	3705.0
19	3360.5
20	3016.0
21	3531.0
22	4046.0
23	4441.0
24	4836.0
25	6347.5
26	9213.0
27	10567.0
28	13186.5
29	15806.0
30	18635.0
31	21464.0
32	23802.0
33	26140.0
34	30436.0
35	34732.0
36	35092.0
37	35452.0
38	37827.5
39	40203.0
40	46413.0
41	52623.0
42	60317.5
43	68012.0
44	78063.5
45	88115.0
46	95582.5
47	103050.0
48	106989.0
49	110928.0
50	102816.5
51	94705.0
52	88005.0
53	81305.0
54	76060.5
55	70816.0
56	70102.0
57	69388.0
58	69162.5
59	68937.0
60	67713.0
61	66489.0
62	61364.5
63	56240.0
64	50484.5
65	44729.0
66	39818.0
67	34907.0
68	31567.0
69	28227.0
70	25197.5
71	22168.0
72	20725.0
73	19282.0
74	15714.5
75	10322.5
76	8498.0
77	6859.5
78	5221.0
79	3776.5
80	2332.0
81	1720.5
82	1109.0
83	931.0
84	753.0
85	560.0
86	367.0
87	274.5
88	182.0
89	124.0
90	66.0
91	49.0
92	32.0
93	25.0
94	18.0
95	12.5
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1346014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.2757711563521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81318583133319	19.65774150381754
2	9.6531575947359	5.072883197909656
3	3.8368610134826135	3.0244944584699502
4	1.9831245894777134	2.0843251155060445
5	1.1970813746469835	1.5727118127877766
6	0.8115248525766977	1.2794064788398614
7	0.5751882404425244	1.057946020438482
8	0.43856190943364753	0.9218841896137071
9	0.36400295163834534	0.8608012431737277
>10	4.675991904053813	30.287292356390598
>50	1.4718237131452057	25.98560919296854
>100	0.17305545003475017	6.363603464772713
>500	0.003920349999197383	0.8023760306448987
>1k	0.0025202249994840317	1.0289249346665075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3240	0.2407107206908695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	0.15259870996884134	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1930	0.14338632436215373	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1375	0.10215346942899553	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.485868646239935E-4	0.0	0.0	0.06158925538664531	0.0
2	1.485868646239935E-4	0.0	0.0	0.35787146344688836	0.0
3	1.485868646239935E-4	0.0	0.0	0.5190882115639214	0.0
4	1.485868646239935E-4	0.0	0.0	0.7406312267182956	0.0
5	1.485868646239935E-4	0.0	0.0	1.3554093791000688	0.0
6	1.485868646239935E-4	0.0	0.0	1.9092669169860046	0.0
7	2.2288029693599026E-4	0.0	0.0	2.351535719539321	0.0
8	2.2288029693599026E-4	0.0	0.0	3.086074884808033	0.0
9	2.2288029693599026E-4	0.0	0.0	3.455313243398657	0.0
10	2.2288029693599026E-4	0.0	0.0	4.1958701766846405	0.0
11	2.2288029693599026E-4	0.0	0.0	5.050987582595724	0.0
12	2.2288029693599026E-4	0.0	0.0	5.749865900354677	0.0
13	2.2288029693599026E-4	0.0	0.0	6.006921176154186	0.0
14	2.2288029693599026E-4	0.0	0.0	6.107068722910757	0.0
15	2.2288029693599026E-4	0.0	0.0	6.285001493297989	0.0
16	2.2288029693599026E-4	0.0	0.0	6.669767179241821	0.0
17	2.2288029693599026E-4	0.0	0.0	7.1006690866514015	0.0
18	2.2288029693599026E-4	0.0	0.0	7.6534865164849695	0.0
19	2.97173729247987E-4	0.0	0.0	7.920051351620414	0.0
20	3.7146716155998376E-4	0.0	0.0	8.175472171909059	0.0
21	3.7146716155998376E-4	0.0	0.0	8.463656395847295	0.0
22	3.7146716155998376E-4	0.0	0.0	8.775465931260745	0.0
23	3.7146716155998376E-4	0.0	0.0	9.090544377695922	0.0
24	0.006017768017271737	0.0	0.0	9.349605576167855	0.0
25	0.006017768017271737	0.0	0.0	9.596854118902181	0.0
26	0.006017768017271737	0.0	0.0	9.800269536572427	0.0
27	0.006092061449583734	0.0	0.0	10.034962489246025	0.0
28	0.006092061449583734	0.0	0.0	10.275450329639959	0.0
29	0.006092061449583734	0.0	0.0	10.547215705037244	0.0
30	0.006092061449583734	0.0	0.0	10.846692530686902	0.0
31	0.006092061449583734	0.0	0.0	11.10857687958669	0.0
32	0.006092061449583734	0.0	0.0	11.384131220031886	0.0
33	0.006092061449583734	0.0	0.0	11.669343706677642	0.0
34	0.006092061449583734	0.0	0.0	11.933605445411414	0.0
35	0.006092061449583734	0.0	0.0	12.228921838851601	0.0
36	0.006092061449583734	0.0	0.0	12.493703631611558	0.0
37	0.006092061449583734	0.0	0.0	12.76613764789965	0.0
38	0.006240648314207727	0.0	0.0	13.051721601706966	0.0
39	0.006314941746519724	0.0	0.0	13.342134628614561	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	25	3.8911618E-5	45.000004	11
AGCCGAT	20	7.033717E-4	45.0	30
CGAAACG	20	7.033717E-4	45.0	3
ACGTATG	40	6.8157533E-9	45.0	1
ATCGTAG	30	2.1657797E-6	44.999996	1
TACGCAT	60	0.0	44.999996	16
CGTTTTA	30	2.1657797E-6	44.999996	40
GCTAACG	65	0.0	41.53846	1
CGTATGG	115	0.0	41.08696	2
TCGTACA	45	1.9286745E-8	40.000004	34
CAATGCG	45	1.9286745E-8	40.000004	1
TCGTGTA	490	0.0	39.94898	17
CGTGTAC	495	0.0	39.545452	18
ATAGCGT	40	3.4588084E-7	39.375	13
CGGTCTA	80	0.0	39.375	31
CGGGATA	155	0.0	39.193546	6
GGTATAC	75	0.0	39.0	8
TCGCTAA	35	6.249087E-6	38.571426	37
AACGCAC	35	6.249087E-6	38.571426	18
CGAATCC	35	6.249087E-6	38.571426	45
>>END_MODULE