SRR3553958_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620678
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1984	0.31965044676950044	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1224	0.19720370304731277	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1076	0.1733588108487815	Illumina PCR Primer Index 8 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1035	0.16675313125324243	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.15031948933263303	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	810	0.1305024505460158	Illumina PCR Primer Index 8 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	659	0.10617421593805483	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	658	0.10601310180157829	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	640	0.10311304734500015	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTA	105	0.0	45.000004	26
GATCACG	35	1.2107193E-7	45.000004	1
CGAACTA	20	7.030777E-4	45.0	43
TTCGTGT	20	7.030777E-4	45.0	44
TAACGCC	55	1.8189894E-12	45.0	12
ACGGATA	20	7.030777E-4	45.0	10
CGCAGAT	20	7.030777E-4	45.0	28
ACCACGT	20	7.030777E-4	45.0	11
ATAGTCG	20	7.030777E-4	45.0	1
ATACGAC	40	6.8066583E-9	45.0	12
TCACGTA	20	7.030777E-4	45.0	41
TACCCGT	25	3.8887258E-5	44.999996	36
AGTATAC	25	3.8887258E-5	44.999996	28
TCTACGA	50	2.1827873E-11	44.999996	22
CGAATAT	60	0.0	44.999996	14
ACCCGTG	25	3.8887258E-5	44.999996	37
CGTTTAT	50	2.1827873E-11	44.999996	39
TACGCAT	120	0.0	43.124996	16
ATCGAGG	110	0.0	42.954544	2
CAACCCG	90	0.0	42.5	23