##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620678
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.772895124364002	31.0	31.0	34.0	30.0	34.0
2	31.886168029155215	33.0	31.0	34.0	30.0	34.0
3	31.899762839991105	33.0	31.0	34.0	30.0	34.0
4	35.56332107791802	37.0	35.0	37.0	33.0	37.0
5	35.40628151795295	37.0	35.0	37.0	33.0	37.0
6	35.34570260263776	37.0	35.0	37.0	33.0	37.0
7	35.70015853631029	37.0	35.0	37.0	35.0	37.0
8	35.597058378096214	37.0	35.0	37.0	35.0	37.0
9	37.349503285117244	39.0	37.0	39.0	34.0	39.0
10	36.75332136792346	39.0	37.0	39.0	32.0	39.0
11	36.63661512088394	39.0	35.0	39.0	32.0	39.0
12	36.48961136047999	38.0	35.0	39.0	32.0	39.0
13	36.334029883450036	39.0	35.0	39.0	32.0	39.0
14	37.38975281869182	39.0	36.0	41.0	32.0	41.0
15	37.551814306290865	39.0	36.0	41.0	32.0	41.0
16	37.609691337537335	39.0	36.0	41.0	33.0	41.0
17	37.552299259841654	39.0	36.0	41.0	32.0	41.0
18	37.516469087030636	39.0	36.0	41.0	32.0	41.0
19	37.45690196849252	39.0	36.0	41.0	32.0	41.0
20	37.35504238912931	39.0	35.0	41.0	32.0	41.0
21	37.255860526714336	39.0	35.0	41.0	32.0	41.0
22	37.223892259754656	39.0	35.0	41.0	32.0	41.0
23	36.726972762044085	39.0	35.0	41.0	31.0	41.0
24	36.83004230857224	39.0	35.0	41.0	31.0	41.0
25	36.82775126555154	39.0	35.0	41.0	31.0	41.0
26	36.81525203084369	39.0	35.0	40.0	31.0	41.0
27	34.407385149787814	38.0	35.0	40.0	23.0	41.0
28	35.270478412316855	38.0	34.0	40.0	22.0	41.0
29	35.933777256484035	38.0	34.0	40.0	27.0	41.0
30	36.19829122346853	38.0	35.0	40.0	30.0	41.0
31	36.230211156187266	38.0	35.0	40.0	30.0	41.0
32	36.0089257231608	38.0	35.0	40.0	30.0	41.0
33	35.996200928661885	38.0	35.0	40.0	29.0	41.0
34	35.91925442822204	38.0	35.0	40.0	29.0	41.0
35	35.84389490202649	38.0	35.0	40.0	29.0	41.0
36	35.699568858570785	38.0	34.0	40.0	28.0	41.0
37	35.6238500478509	38.0	34.0	40.0	28.0	41.0
38	35.505453713519735	38.0	34.0	40.0	28.0	41.0
39	35.367560957533534	38.0	34.0	40.0	27.0	41.0
40	35.22849206835106	38.0	34.0	40.0	27.0	41.0
41	35.11903273517025	38.0	34.0	40.0	26.0	41.0
42	35.01535417720622	37.0	34.0	40.0	26.0	41.0
43	34.92028555869549	37.0	34.0	40.0	26.0	41.0
44	34.79219982019662	37.0	33.0	40.0	26.0	41.0
45	34.669877134359524	37.0	33.0	40.0	25.0	41.0
46	34.501063353300744	37.0	33.0	40.0	24.0	41.0
47	34.36019320807246	36.0	33.0	40.0	24.0	41.0
48	34.255264404409374	36.0	33.0	40.0	24.0	41.0
49	34.205865199024295	36.0	33.0	40.0	24.0	41.0
50	34.07837880511312	36.0	33.0	40.0	24.0	41.0
51	32.46276330077754	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	4.0
12	6.0
13	11.0
14	24.0
15	34.0
16	78.0
17	223.0
18	349.0
19	661.0
20	1053.0
21	1741.0
22	2590.0
23	3395.0
24	4334.0
25	5773.0
26	7220.0
27	8817.0
28	10636.0
29	13081.0
30	15501.0
31	19659.0
32	25207.0
33	33196.0
34	49746.0
35	59427.0
36	60343.0
37	80538.0
38	106822.0
39	110188.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44639571565288	18.83633703788438	23.830714154521345	12.886553091941394
2	31.041377332529912	24.699441578402972	26.668256326146565	17.590924762920547
3	32.6596077193005	23.88710410228814	26.702573637216076	16.750714541195276
4	28.396688782267134	27.050419057868975	25.564141148872682	18.988751010991205
5	24.43038097048711	31.366989002349044	24.174209493489375	20.028420533674467
6	25.1845562433339	36.51506900518465	24.130225334231277	14.170149417250169
7	82.50316589278177	6.069008406935642	7.43396737116508	3.993858329117513
8	83.66931001259913	4.830201811567351	6.943535939730423	4.5569522361031005
9	77.79428302598127	7.593631480413354	9.245695835844028	5.366389657761352
10	42.56216588955948	23.612404499595606	16.173120361926795	17.65230924891812
11	31.64474977363464	25.23015154395677	22.22746738244307	20.89763129996552
12	27.45272105665095	22.427893368219916	29.755042066901034	20.3643435082281
13	23.97781136112445	24.217065853792143	30.764099903653747	21.041022881429665
14	21.936817480239352	27.68053644562881	29.484853660029835	20.897792414102
15	20.440067152372084	24.860877943152488	32.55262793268007	22.14642697179536
16	23.840542116846418	21.945517643609087	30.327802822075217	23.886137417469282
17	23.50043017474439	22.332030457016362	27.678119733581664	26.489419634657587
18	24.439242247993324	23.168051711193243	28.934648883962378	23.458057156851055
19	24.91597897782747	25.40077141448545	25.950331734007005	23.732917873680073
20	26.043616819026933	24.93209039147513	25.798723331582558	23.225569457915377
21	25.335520189212442	25.785350858255008	27.350091351715385	21.529037600817173
22	24.06304073932055	23.11295067651826	27.004179300700205	25.819829283460987
23	22.042025011358547	27.32576311710742	25.61634212909109	25.01586974244294
24	23.376050061384486	24.822210550398115	27.59772377947986	24.204015608737542
25	23.101833801101375	25.624720064187873	25.88266379668685	25.390782338023904
26	21.711257689172164	26.35231150451603	26.47846387337718	25.457966932934628
27	21.873338510467587	30.4317214401026	24.492893255440016	23.202046793989798
28	22.719026612833062	25.26446885502628	27.54326720135078	24.47323733078988
29	24.274261372241323	25.863974556855567	25.868324638540436	23.993439432362674
30	24.94659066375802	25.871063578860536	25.80371786981333	23.37862788756811
31	26.86417111610207	26.857726550643008	23.0398048585579	23.238297474697024
32	27.060408134330523	26.40257911509672	23.57454267752361	22.96247007304915
33	26.58802148618124	24.5913339928272	23.127289834664673	25.693354686326884
34	24.80497133779512	26.381150934945335	24.941596125527248	23.872281601732297
35	26.337972346369614	25.931481380039244	23.878887281327838	23.8516589922633
36	27.579678996194485	27.05251354164317	23.174979619061737	22.19282784310061
37	26.814709076203762	26.95938957075972	24.33774034201309	21.88816101102343
38	27.010301637886315	27.154337675896357	22.436915759862604	23.398444926354728
39	25.109799284008776	26.104034620205645	23.471429630178612	25.314736465606963
40	25.830140588195487	25.57880253529205	25.43009418732418	23.160962689188274
41	24.20965460351422	24.98219688791934	24.910662211323746	25.897486297242693
42	23.96363331711451	25.375798723331584	25.076448657758128	25.584119301795777
43	25.38949342493209	23.403761692858456	25.59523617721266	25.611508704996794
44	24.43424770976255	24.576833720544307	26.06778393949842	24.92113463019472
45	25.51886807652277	24.495309967487167	25.50807342937884	24.477748526611222
46	26.111445870483568	25.280258040400984	25.633903569967035	22.974392519148417
47	23.6894170568314	26.534209364598066	26.251937397491133	23.524436181079402
48	24.219643679975768	25.359365081410974	26.472019307918117	23.948971930695144
49	25.687715691550206	23.792852332449353	26.723196246685077	23.796235729315363
50	24.10895826821637	23.861486954588372	27.07748623279704	24.952068544398223
51	23.65703311539961	24.412980643747645	25.143633252668856	26.786352988183886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2232.0
1	2411.5
2	2591.0
3	2648.0
4	2705.0
5	2044.5
6	1384.0
7	1372.5
8	1361.0
9	1438.5
10	1516.0
11	1576.5
12	1637.0
13	1726.0
14	1815.0
15	1684.0
16	1553.0
17	1718.0
18	1883.0
19	1803.5
20	1724.0
21	1810.0
22	1896.0
23	1921.0
24	1946.0
25	2398.5
26	3632.5
27	4414.0
28	4792.0
29	5170.0
30	6096.0
31	7022.0
32	8283.0
33	9544.0
34	10814.0
35	12084.0
36	12729.0
37	13374.0
38	14748.0
39	16122.0
40	17728.0
41	19334.0
42	22192.5
43	25051.0
44	28037.0
45	31023.0
46	34411.5
47	37800.0
48	42308.0
49	46816.0
50	50969.0
51	55122.0
52	55550.0
53	55978.0
54	51042.5
55	46107.0
56	41482.5
57	36858.0
58	35273.5
59	33689.0
60	31974.0
61	30259.0
62	27423.0
63	24587.0
64	22331.0
65	20075.0
66	17907.5
67	15740.0
68	14294.5
69	12849.0
70	11597.5
71	10346.0
72	9508.5
73	8671.0
74	7445.5
75	5013.5
76	3807.0
77	2995.0
78	2183.0
79	1847.5
80	1512.0
81	1242.0
82	972.0
83	722.0
84	472.0
85	337.5
86	203.0
87	149.0
88	95.0
89	68.0
90	41.0
91	25.0
92	9.0
93	15.5
94	22.0
95	16.0
96	10.0
97	6.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.61180248600396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05225528336051	24.04148872632743
2	9.858219302578213	6.232721629136284
3	3.5811059390130353	3.396156408766074
4	1.8423445474892775	2.3295932778558956
5	1.136233924290451	1.7959201196283456
6	0.7381194197588468	1.3999971185100306
7	0.565779709979848	1.2519721509730088
8	0.40953132495470496	1.0356818685039708
9	0.3417446140030185	0.9722846914667174
>10	4.509435749411607	34.488180654290794
>50	0.8893870590347722	18.218571005218937
>100	0.07078691771735834	3.3018892275666367
>500	0.0030337250450296433	0.6853761742572242
>1k	0.0020224833633530953	0.8501669474986416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1984	0.31965044676950044	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1224	0.19720370304731277	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	1076	0.1733588108487815	Illumina PCR Primer Index 8 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1035	0.16675313125324243	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.15031948933263303	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	810	0.1305024505460158	Illumina PCR Primer Index 8 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	659	0.10617421593805483	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	658	0.10601310180157829	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	640	0.10311304734500015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1939814203177815	0.0
2	0.0	0.0	0.0	0.7277525544646338	0.0
3	0.0	0.0	0.0	0.9301119098791967	0.0
4	0.0	0.0	0.0	1.208194909437744	0.0
5	0.0	0.0	0.0	1.9721981446096044	0.0
6	0.0	0.0	0.0	2.5705760474835584	0.0
7	0.0	0.0	0.0	3.1552592487570044	0.0
8	0.0	0.0	0.0	4.205078962038288	0.0
9	0.0	0.0	0.0	4.6636097944505845	0.0
10	0.0	0.0	0.0	5.735018802019727	0.0
11	0.0	0.0	0.0	7.048582356713143	0.0
12	0.0	0.0	0.0	8.11225788573141	0.0
13	0.0	0.0	0.0	8.517137710697012	0.0
14	0.0	0.0	0.0	8.684051956086732	0.0
15	0.0	0.0	0.0	8.970835119015012	0.0
16	0.0	0.0	0.0	9.587257805174342	0.0
17	0.0	0.0	0.0	10.268609488333725	0.0
18	0.0	0.0	0.0	11.09335275295725	0.0
19	0.0	0.0	0.0	11.50773831197497	0.0
20	0.0	0.0	0.0	11.880878652054689	0.0
21	0.0	0.0	0.0	12.321525815318088	0.0
22	0.0	0.0	0.0	12.747833820435073	0.0
23	1.6111413647656273E-4	0.0	0.0	13.213614788988815	0.0
24	3.2222827295312547E-4	0.0	0.0	13.590460754207495	0.0
25	3.2222827295312547E-4	0.0	0.0	13.92026139157502	0.0
26	3.2222827295312547E-4	0.0	0.0	14.245389718984724	0.0
27	4.8334240942968817E-4	0.0	0.0	14.5858238893597	0.0
28	4.8334240942968817E-4	0.0	0.0	14.946397326794248	0.0
29	4.8334240942968817E-4	0.0	0.0	15.312770873141952	0.0
30	4.8334240942968817E-4	0.0	0.0	15.703150425824663	0.0
31	4.8334240942968817E-4	0.0	0.0	16.0576015260731	0.0
32	4.8334240942968817E-4	0.0	0.0	16.408347001182577	0.0
33	4.8334240942968817E-4	0.0	0.0	16.78277625435411	0.0
34	4.8334240942968817E-4	0.0	0.0	17.148022001746476	0.0
35	4.8334240942968817E-4	0.0	0.0	17.54951842984607	0.0
36	6.444565459062509E-4	0.0	0.0	17.912508579327767	0.0
37	6.444565459062509E-4	0.0	0.0	18.264704081665535	0.0
38	6.444565459062509E-4	0.0	0.0	18.635911052107534	0.0
39	6.444565459062509E-4	0.0	0.0	19.028546202700916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTA	105	0.0	45.000004	26
GATCACG	35	1.2107193E-7	45.000004	1
CGAACTA	20	7.030777E-4	45.0	43
TTCGTGT	20	7.030777E-4	45.0	44
TAACGCC	55	1.8189894E-12	45.0	12
ACGGATA	20	7.030777E-4	45.0	10
CGCAGAT	20	7.030777E-4	45.0	28
ACCACGT	20	7.030777E-4	45.0	11
ATAGTCG	20	7.030777E-4	45.0	1
ATACGAC	40	6.8066583E-9	45.0	12
TCACGTA	20	7.030777E-4	45.0	41
TACCCGT	25	3.8887258E-5	44.999996	36
AGTATAC	25	3.8887258E-5	44.999996	28
TCTACGA	50	2.1827873E-11	44.999996	22
CGAATAT	60	0.0	44.999996	14
ACCCGTG	25	3.8887258E-5	44.999996	37
CGTTTAT	50	2.1827873E-11	44.999996	39
TACGCAT	120	0.0	43.124996	16
ATCGAGG	110	0.0	42.954544	2
CAACCCG	90	0.0	42.5	23
>>END_MODULE