FastQCFastQC Report
Sat 18 Jun 2016
SRR3553957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726492
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20080.27639671187019266No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC18540.25519895607935117TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC16240.2235399701579646No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC14590.20082808895349158No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG14580.20069044118861595No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT12120.16682909102921986TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC11230.154578439955292TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.15375255336603844No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10110.1391618902892255No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8640.11892766885251319No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7790.10722760883808768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG302.1643937E-645.0000041
ATATCCG302.1643937E-645.00000422
CGGTCGA253.8893828E-545.00000441
CATTTCG302.1643937E-645.00000413
CGCCAAA253.8893828E-545.00000443
TTCTACG207.031571E-445.019
CACGGAT207.031571E-445.034
TCCGACA207.031571E-445.025
CCTGTCG207.031571E-445.037
AACGCAC207.031571E-445.018
CATACCG207.031571E-445.01
ACCGTAA207.031571E-445.017
CTTTACG207.031571E-445.037
CGACTTA351.2111013E-745.011
ATCGGCT207.031571E-445.017
TATACGA207.031571E-445.013
TGTAACG351.2111013E-745.01
GCGTAGC207.031571E-445.01
TCGAACG207.031571E-445.01
TAGTATC207.031571E-445.025