##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726492
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775844744333043	31.0	31.0	34.0	30.0	34.0
2	31.89362718378179	33.0	31.0	34.0	30.0	34.0
3	31.90248619392918	33.0	31.0	34.0	30.0	34.0
4	35.56718725051343	37.0	35.0	37.0	33.0	37.0
5	35.397716698876245	37.0	35.0	37.0	33.0	37.0
6	35.34901279023031	37.0	35.0	37.0	33.0	37.0
7	35.65275736002599	37.0	35.0	37.0	35.0	37.0
8	35.509110905557115	37.0	35.0	37.0	33.0	37.0
9	37.298322624337224	39.0	37.0	39.0	34.0	39.0
10	36.72842371285575	39.0	37.0	39.0	32.0	39.0
11	36.65060317250568	39.0	35.0	39.0	32.0	39.0
12	36.514601674898	38.0	35.0	39.0	32.0	39.0
13	36.41023301013638	39.0	35.0	39.0	32.0	39.0
14	37.47695363472688	39.0	36.0	41.0	32.0	41.0
15	37.59746287639781	39.0	36.0	41.0	33.0	41.0
16	37.64702570709657	39.0	36.0	41.0	33.0	41.0
17	37.54988079703562	39.0	36.0	41.0	32.0	41.0
18	37.548490554610375	39.0	36.0	41.0	32.0	41.0
19	37.47718488297187	39.0	36.0	41.0	32.0	41.0
20	37.381916111946175	39.0	35.0	41.0	32.0	41.0
21	37.278110426542895	39.0	35.0	41.0	32.0	41.0
22	37.27171668786442	39.0	35.0	41.0	32.0	41.0
23	36.796545591692684	39.0	35.0	41.0	31.0	41.0
24	36.88573446094382	39.0	35.0	41.0	31.0	41.0
25	36.87833727005941	39.0	35.0	41.0	31.0	41.0
26	36.87692362751414	39.0	35.0	40.0	31.0	41.0
27	34.629342098743	38.0	35.0	40.0	24.0	41.0
28	35.44241368108665	38.0	35.0	40.0	24.0	41.0
29	36.093322431630355	38.0	35.0	40.0	29.0	41.0
30	36.338595607384526	38.0	35.0	40.0	30.0	41.0
31	36.41647946570644	38.0	35.0	40.0	30.0	41.0
32	36.20060234661909	38.0	35.0	40.0	30.0	41.0
33	36.18005015884552	38.0	35.0	40.0	30.0	41.0
34	36.134258876904354	38.0	35.0	40.0	30.0	41.0
35	36.05627315923644	38.0	35.0	40.0	30.0	41.0
36	35.92580785473206	38.0	35.0	40.0	29.0	41.0
37	35.869212599725806	38.0	35.0	40.0	29.0	41.0
38	35.73435495504424	38.0	34.0	40.0	29.0	41.0
39	35.647406991405276	38.0	34.0	40.0	28.0	41.0
40	35.48886842525451	38.0	34.0	40.0	28.0	41.0
41	35.37974265373879	38.0	34.0	40.0	27.0	41.0
42	35.279901223963925	38.0	34.0	40.0	27.0	41.0
43	35.192152150333385	38.0	34.0	40.0	27.0	41.0
44	35.10485456137163	37.0	34.0	40.0	26.0	41.0
45	35.034253646289294	37.0	34.0	40.0	26.0	41.0
46	34.87765866657857	37.0	34.0	40.0	26.0	41.0
47	34.744517489525	37.0	33.0	40.0	26.0	41.0
48	34.663076812958714	37.0	33.0	40.0	26.0	41.0
49	34.61227515237608	37.0	33.0	40.0	26.0	41.0
50	34.49150300347423	36.0	33.0	40.0	25.0	41.0
51	32.81487889749646	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	5.0
12	7.0
13	19.0
14	23.0
15	54.0
16	139.0
17	213.0
18	437.0
19	724.0
20	1253.0
21	1973.0
22	2741.0
23	3685.0
24	4683.0
25	6113.0
26	7636.0
27	9389.0
28	11493.0
29	13993.0
30	17783.0
31	22403.0
32	28807.0
33	38181.0
34	57008.0
35	67142.0
36	70513.0
37	93778.0
38	127645.0
39	138624.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.136629171415514	18.517478513183903	23.340931489954468	13.004960825446116
2	31.110321930592487	26.35954697367624	25.420238626165187	17.10989246956608
3	32.62320851434015	25.52705329170865	25.71040011452294	16.139338079428267
4	28.44835180566338	26.73105829107547	26.003452205943077	18.81713769731807
5	24.430275901179915	31.246868513349078	24.44197596119434	19.880879624276663
6	23.554973764336015	38.275851626721284	23.729924073492896	14.439250535449805
7	80.82497811400539	6.530973500052307	8.195823216222616	4.448225169719694
8	82.16993442460482	6.227047235206995	6.764424109281314	4.838594230906878
9	76.6930675079698	7.32877994527125	9.096177246273875	6.88197530048507
10	41.888279568116374	24.952098577823293	16.35035210298255	16.80926975107778
11	31.856785759512835	26.107100972894404	21.50126911239215	20.534844155200606
12	27.610076917571014	22.36294412051337	28.851522108983996	21.175456852931624
13	25.42670807111434	23.77906432555348	30.424010174922778	20.370217428409397
14	21.684891230736195	28.261013197667694	27.956123398468257	22.097972173127854
15	21.197618143076593	25.341641752421225	31.65458119291059	21.806158911591595
16	23.69317212027111	24.022287926088655	29.11497993095588	23.16956002268435
17	22.555650991339203	24.61788980470535	27.342352014887982	25.484107189067462
18	23.68326148120007	24.329380089526108	27.99260005616029	23.994758373113537
19	24.233164301878066	27.389840493770063	25.426570423349464	22.950424781002408
20	26.081085545332915	25.396976153901214	25.96876496919443	22.55317333157144
21	25.407575031796636	27.404155861317125	26.07599257803252	21.112276528853723
22	22.819246461075966	24.473222003821103	25.801109991575956	26.906421543526974
23	23.196538984599968	27.917444376538214	25.249280102189704	23.636736536672117
24	22.883940910567492	26.2920995688872	26.830027034021022	23.993932486524283
25	23.86784713389824	26.690589848202045	24.938884392395234	24.50267862550448
26	21.639605116092124	26.499121807260096	26.39987776878479	25.46139530786299
27	21.88833462722232	30.455256217549536	24.089735330877698	23.56667382435044
28	21.45240415586132	27.454810238791342	27.6186110789933	23.47417452635404
29	23.09867142377342	26.153350621892603	26.4827417232399	24.26523623109408
30	23.56144320928517	25.694708269327123	27.060862335717395	23.68298618567032
31	25.75609917246164	26.705318159043735	23.250634556196076	24.28794811229855
32	25.405647963088374	26.577168089944553	24.88974414033465	23.12743980663242
33	25.047350831117203	25.08781927399063	24.105289528308642	25.75954036658353
34	23.5984704580367	25.74880384092323	26.348397504721316	24.304328196318746
35	24.375079147464803	23.443341427021906	26.86595310065355	25.315626324859736
36	24.817479063774964	26.90339329269971	25.568072325641577	22.711055317883748
37	24.926083150261807	26.979650154440794	25.519482664640492	22.57478403065691
38	24.664277101468425	27.685094949428212	25.67502463894991	21.97560331015345
39	26.36477758874151	24.2527102844904	25.677502298717673	23.705009828050414
40	24.939847926749366	23.033564030987264	29.732880747482422	22.293707294780948
41	23.20576138484663	23.924282717497235	27.65274772468245	25.217208172973688
42	23.438110811956637	24.934617311684093	27.154187520303047	24.473084356056226
43	23.703633350401656	23.51285354828408	27.06416588207441	25.71934721923985
44	24.409628736448578	23.829168111968198	27.11096612213211	24.650237029451116
45	24.76943999383338	23.521525357471244	26.695545167737567	25.01348948095781
46	24.763521139943727	25.10750290436784	26.632364843659666	23.496611112028763
47	23.654630746105944	24.52759287094696	28.459088331323677	23.358688051623417
48	23.376169317762617	24.64569465321022	27.71482686664134	24.26330916238582
49	24.09303887723471	24.585955523254214	27.981175291675616	23.33983030783546
50	23.22090263898295	23.158135258199678	28.8577162584034	24.763245844413976
51	22.797773409755372	23.795582057338553	26.805250436343414	26.601394096562657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2295.0
1	2473.5
2	2652.0
3	2849.0
4	3046.0
5	2283.5
6	1521.0
7	1543.0
8	1565.0
9	1606.5
10	1648.0
11	1651.5
12	1655.0
13	1628.0
14	1601.0
15	1727.0
16	1853.0
17	1808.5
18	1764.0
19	1815.5
20	1867.0
21	1911.0
22	1955.0
23	2423.5
24	2892.0
25	3050.0
26	3740.5
27	4273.0
28	6192.5
29	8112.0
30	8743.5
31	9375.0
32	10750.0
33	12125.0
34	13511.5
35	14898.0
36	15249.0
37	15600.0
38	17856.5
39	20113.0
40	22295.0
41	24477.0
42	28222.0
43	31967.0
44	36794.5
45	41622.0
46	51435.0
47	61248.0
48	65329.0
49	69410.0
50	67647.5
51	65885.0
52	59635.0
53	53385.0
54	48054.0
55	42723.0
56	40927.5
57	39132.0
58	36579.5
59	34027.0
60	34075.0
61	34123.0
62	31202.0
63	28281.0
64	24718.0
65	21155.0
66	19309.0
67	17463.0
68	15611.5
69	13760.0
70	12345.5
71	10931.0
72	9533.5
73	8136.0
74	6949.5
75	4753.0
76	3743.0
77	2957.0
78	2171.0
79	1803.5
80	1436.0
81	1072.0
82	708.0
83	581.0
84	454.0
85	343.0
86	232.0
87	161.0
88	90.0
89	70.5
90	51.0
91	42.0
92	33.0
93	38.0
94	43.0
95	33.5
96	24.0
97	12.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.674994723790324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3165003319012	24.17324745351113
2	9.52641725900191	6.034984328310211
3	3.5426153397487203	3.366369665848781
4	1.7208477180262118	2.180313695557075
5	1.051540992858092	1.6653777700314651
6	0.7200144325863049	1.3683872011934455
7	0.5474563548947639	1.2138474006957989
8	0.43420023635267546	1.1002632156431607
9	0.36523286805299776	1.041187425168016
>10	5.022084816105109	38.75592697615817
>50	0.693184791926248	13.791742028934252
>100	0.05348648084441618	2.9870496915075986
>500	0.0025673510805319768	0.5775134414226544
>1k	0.003851026620797965	1.7437897060182752
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2008	0.27639671187019266	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1854	0.25519895607935117	TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	1624	0.2235399701579646	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	1459	0.20082808895349158	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	1458	0.20069044118861595	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1212	0.16682909102921986	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1123	0.154578439955292	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1117	0.15375255336603844	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1011	0.1391618902892255	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	864	0.11892766885251319	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	779	0.10722760883808768	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3764776487559395E-4	0.0	0.0	0.17894209433827213	0.0
2	1.3764776487559395E-4	0.0	0.0	0.7647709816488	0.0
3	1.3764776487559395E-4	0.0	0.0	1.0181805167847684	0.0
4	1.3764776487559395E-4	0.0	0.0	1.3818459115860877	0.0
5	1.3764776487559395E-4	0.0	0.0	2.483165678355715	0.0
6	1.3764776487559395E-4	0.0	0.0	3.290332171586198	0.0
7	1.3764776487559395E-4	0.0	0.0	3.9718262554852632	0.0
8	1.3764776487559395E-4	0.0	0.0	5.193587816521036	0.0
9	1.3764776487559395E-4	0.0	0.0	5.744867114847789	0.0
10	1.3764776487559395E-4	0.0	0.0	7.119280047130594	0.0
11	1.3764776487559395E-4	0.0	0.0	8.440010351111919	0.0
12	1.3764776487559395E-4	0.0	0.0	9.672233142278236	0.0
13	1.3764776487559395E-4	0.0	0.0	10.111467159996256	0.0
14	1.3764776487559395E-4	0.0	0.0	10.298943415756815	0.0
15	1.3764776487559395E-4	0.0	0.0	10.618286230268193	0.0
16	1.3764776487559395E-4	0.0	0.0	11.232608204907969	0.0
17	1.3764776487559395E-4	0.0	0.0	11.911211685744647	0.0
18	1.3764776487559395E-4	0.0	0.0	12.760911338321689	0.0
19	1.3764776487559395E-4	0.0	0.0	13.210193642875627	0.0
20	1.3764776487559395E-4	0.0	0.0	13.591477951581021	0.0
21	1.3764776487559395E-4	0.0	0.0	14.047917939908492	0.0
22	1.3764776487559395E-4	0.0	0.0	14.494447289164919	0.0
23	2.752955297511879E-4	0.0	0.0	14.960109677739053	0.0
24	2.752955297511879E-4	0.0	0.0	15.351166977750616	0.0
25	4.1294329462678183E-4	0.0	0.0	15.672299213205376	0.0
26	4.1294329462678183E-4	0.0	0.0	15.967278373333773	0.0
27	4.1294329462678183E-4	0.0	0.0	16.28758472219928	0.0
28	4.1294329462678183E-4	0.0	0.0	16.62069231319822	0.0
29	4.1294329462678183E-4	0.0	0.0	16.977200024226008	0.0
30	4.1294329462678183E-4	0.0	0.0	17.368257324237568	0.0
31	4.1294329462678183E-4	0.0	0.0	17.70274139288526	0.0
32	4.1294329462678183E-4	0.0	0.0	18.048374930487878	0.0
33	4.1294329462678183E-4	0.0	0.0	18.40942501775656	0.0
34	4.1294329462678183E-4	0.0	0.0	18.759187988305445	0.0
35	4.1294329462678183E-4	0.0	0.0	19.122853383106765	0.0
36	4.1294329462678183E-4	0.0	0.0	19.46284336234948	0.0
37	4.1294329462678183E-4	0.0	0.0	19.802695693827324	0.0
38	4.1294329462678183E-4	0.0	0.0	20.141309195421286	0.0
39	5.505910595023758E-4	0.0	0.0	20.491485109264797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	30	2.1643937E-6	45.000004	1
ATATCCG	30	2.1643937E-6	45.000004	22
CGGTCGA	25	3.8893828E-5	45.000004	41
CATTTCG	30	2.1643937E-6	45.000004	13
CGCCAAA	25	3.8893828E-5	45.000004	43
TTCTACG	20	7.031571E-4	45.0	19
CACGGAT	20	7.031571E-4	45.0	34
TCCGACA	20	7.031571E-4	45.0	25
CCTGTCG	20	7.031571E-4	45.0	37
AACGCAC	20	7.031571E-4	45.0	18
CATACCG	20	7.031571E-4	45.0	1
ACCGTAA	20	7.031571E-4	45.0	17
CTTTACG	20	7.031571E-4	45.0	37
CGACTTA	35	1.2111013E-7	45.0	11
ATCGGCT	20	7.031571E-4	45.0	17
TATACGA	20	7.031571E-4	45.0	13
TGTAACG	35	1.2111013E-7	45.0	1
GCGTAGC	20	7.031571E-4	45.0	1
TCGAACG	20	7.031571E-4	45.0	1
TAGTATC	20	7.031571E-4	45.0	25
>>END_MODULE