FastQCFastQC Report
Sat 18 Jun 2016
SRR3553956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383774
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41930.3030119080138809No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC26810.19374551046630448No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC23750.17163207286739018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23060.16664570948724286No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT19680.14221975553811533Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA551.8189894E-1245.00000414
ACGATAG253.8912185E-545.0000041
TATCGAG253.8912185E-545.0000041
GTTACGT207.033786E-445.036
CGTAATC207.033786E-445.029
TAATGCG900.042.51
GGTTACG650.041.5384641
TCGTTAG556.184564E-1140.9090921
GACACGA6100.040.20491825
CATACGA3200.040.07812518
ACATACG3200.040.07812517
CCCTCGT1850.038.9189214
ATACGAA3300.038.86363619
TCGATAG356.2492127E-638.571431
CCGTAAG356.2492127E-638.571431
TGTTACG356.2492127E-638.571431
TATCGTG356.2492127E-638.571431
ACGACCA6800.038.3823528
ACACGAC6400.038.32031226
TACGAAA3350.038.28358520
Produced by FastQC (version 0.11.5)