FastQCFastQC Report
Sat 18 Jun 2016
SRR3553955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337091
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC23750.17762441000649917No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC19480.14568941081796227No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.14172558187886988No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT18490.13828527751663874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG406.8157533E-945.0000041
TATTACG253.8911487E-545.01
TCATCCG253.8911487E-545.029
ATCGATC302.1657688E-644.99999613
TACGTCG302.1657688E-644.9999961
TCGTTTA700.041.78571338
CGTAAGG2400.041.2499962
TCGATTA451.9286745E-840.041
TGTTACG451.9286745E-840.01
CAGTACG403.4587902E-739.3750041
AACACGT4200.038.57142641
CGAATAT1000.038.2514
CAACGAG4900.037.6530614
TACGCAT601.5643309E-1037.49999616
ATCGGCC301.14002185E-437.49999633
AACGAGC5000.036.89999815
GTCGAAC552.750312E-936.8181810
AGTACGG1900.036.7105262
TACGTAG800.036.5625041
GACACGA4500.036.525
Produced by FastQC (version 0.11.5)