##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337091
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01355554707944	33.0	31.0	34.0	30.0	34.0
2	32.11620450664913	33.0	31.0	34.0	30.0	34.0
3	32.144447161786296	34.0	31.0	34.0	30.0	34.0
4	35.72089483812246	37.0	35.0	37.0	33.0	37.0
5	35.6011819689161	37.0	35.0	37.0	33.0	37.0
6	35.59951267340817	37.0	35.0	37.0	33.0	37.0
7	35.87218895348185	37.0	35.0	37.0	35.0	37.0
8	35.73191203889638	37.0	35.0	37.0	35.0	37.0
9	37.40445265131543	39.0	37.0	39.0	35.0	39.0
10	36.94727284829529	39.0	37.0	39.0	33.0	39.0
11	36.892751503076454	39.0	37.0	39.0	33.0	39.0
12	36.83604182512634	39.0	35.0	39.0	33.0	39.0
13	36.83088136858299	39.0	37.0	39.0	33.0	39.0
14	37.94675081950294	40.0	37.0	41.0	33.0	41.0
15	38.06835286453951	40.0	37.0	41.0	33.0	41.0
16	38.111951243408264	40.0	37.0	41.0	33.0	41.0
17	38.06317819804337	40.0	37.0	41.0	33.0	41.0
18	38.01807206839325	40.0	37.0	41.0	33.0	41.0
19	37.98828875521561	40.0	37.0	41.0	33.0	41.0
20	37.93089475585431	40.0	36.0	41.0	33.0	41.0
21	37.81370826667744	40.0	36.0	41.0	33.0	41.0
22	37.81131725514569	40.0	36.0	41.0	33.0	41.0
23	37.748773269732574	40.0	36.0	41.0	33.0	41.0
24	37.725842893266055	40.0	36.0	41.0	33.0	41.0
25	37.614943186365025	40.0	36.0	41.0	33.0	41.0
26	37.567846915430586	40.0	36.0	41.0	33.0	41.0
27	37.519281036219674	39.0	36.0	41.0	33.0	41.0
28	37.424365282542475	39.0	36.0	41.0	32.0	41.0
29	37.39870958670726	39.0	36.0	41.0	32.0	41.0
30	37.26533721339834	39.0	35.0	41.0	32.0	41.0
31	37.163860200988566	39.0	35.0	41.0	32.0	41.0
32	37.077667114654126	39.0	35.0	41.0	31.0	41.0
33	36.96616610238196	39.0	35.0	41.0	31.0	41.0
34	36.88332581701619	39.0	35.0	41.0	31.0	41.0
35	36.81491312109647	39.0	35.0	41.0	31.0	41.0
36	36.69674464939185	39.0	35.0	41.0	31.0	41.0
37	36.640484454685584	39.0	35.0	41.0	30.0	41.0
38	36.53032441322244	39.0	35.0	41.0	30.0	41.0
39	36.47099337292675	39.0	35.0	41.0	30.0	41.0
40	36.37175480202918	39.0	35.0	41.0	30.0	41.0
41	36.25633333856858	39.0	35.0	40.0	30.0	41.0
42	36.12075842257558	38.0	35.0	40.0	30.0	41.0
43	36.01036129926834	38.0	35.0	40.0	29.0	41.0
44	35.88662177817366	38.0	35.0	40.0	29.0	41.0
45	35.820757151158745	38.0	35.0	40.0	29.0	41.0
46	35.689850578606844	38.0	34.0	40.0	28.0	41.0
47	35.591354664716164	38.0	34.0	40.0	28.0	41.0
48	35.479700334532204	38.0	34.0	40.0	28.0	41.0
49	35.37158278681107	38.0	34.0	40.0	28.0	41.0
50	35.2513740650412	37.0	34.0	40.0	27.0	41.0
51	33.701688217181925	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	9.0
11	16.0
12	15.0
13	20.0
14	28.0
15	69.0
16	157.0
17	267.0
18	513.0
19	836.0
20	1405.0
21	2112.0
22	3173.0
23	4650.0
24	6376.0
25	8614.0
26	10786.0
27	13643.0
28	16059.0
29	19557.0
30	24536.0
31	31477.0
32	40392.0
33	54627.0
34	87845.0
35	109303.0
36	115187.0
37	165110.0
38	266921.0
39	353289.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64863049710154	19.002446355558448	23.697713917751297	14.65120922958871
2	29.028764683929513	25.394457071358644	26.960693026877003	18.616085217834836
3	32.4972645840859	24.396320070960016	26.15252065865375	16.953894686300334
4	28.211094084097493	26.27637161569407	26.09164222928731	19.420892070921127
5	25.17188433696734	30.876507283348705	24.02506635674012	19.92654202294384
6	25.081538952846145	36.24764507426944	23.683279597274982	14.987536375609439
7	83.86100871219685	5.392976244698379	6.571430067213077	4.174584975891693
8	84.97379759492809	4.615243091158343	6.0262166150247065	4.384742698888856
9	78.7488660083719	7.417595361871405	8.432784305630657	5.400754324126032
10	39.41938132857076	29.67277470269413	15.665126756518442	15.24271721221667
11	29.74928408014114	27.160754204463274	23.841907544063943	19.248054171331646
12	26.195599252406904	23.915799298626645	28.67074866258168	21.21785278638477
13	24.503866976892372	25.0048799969486	30.145143449473522	20.346109576685507
14	21.737413534306942	28.169735642525453	28.261651600377235	21.83119922279037
15	20.63569345691505	26.46289594350721	31.721176793501716	21.180233806076025
16	23.785441678988192	25.642009406988752	29.577642808155915	20.994906105867138
17	23.367369909751844	25.289677366761126	27.78217787719759	23.560774846289444
18	24.442988547525935	24.9662887567114	28.19800596967596	22.392716726086707
19	24.832490832710715	27.14624509476169	25.828234577900833	22.193029494626767
20	25.385931099678334	27.117152086133256	25.489514176671594	22.00740263751682
21	24.97459036071591	27.78636607381248	26.18236155953484	21.056682005936768
22	24.058422351208705	24.95918378031114	25.6986996397403	25.283694228739854
23	22.589636756211807	27.429471890843633	26.159027321251884	23.821864031692684
24	22.47244204022015	26.414507314760176	27.5742638309584	23.53878681406127
25	23.411645131109253	27.601262741279392	25.93391175320154	23.05318037440982
26	22.86702999272301	27.666030210359654	26.014160591911846	23.452779205005495
27	23.90809600842426	27.114684041699483	25.884700442976584	23.09251950689968
28	22.181437164710555	27.728329635006144	26.78007704785987	23.310156152423435
29	23.782674477653355	25.953431740995935	26.243090410450748	24.020803370899962
30	24.79375001402298	26.082742311480672	26.32064683705148	22.80286083744487
31	25.698924007416103	26.979764279319806	23.589867854917877	23.731443858346214
32	25.29782939231511	27.358496916066294	23.46669000090495	23.876983690713647
33	24.069565945773324	27.200392493854196	23.99642208346328	24.7336194769092
34	23.60093666025723	26.84544283074226	25.496245206945527	24.057375302054986
35	23.675875463973657	26.028370544712367	24.737284148947232	25.558469842366748
36	23.925821054812275	28.716295300768607	24.455179191244277	22.90270445317484
37	23.73338837820313	27.407633437065986	24.728608598816386	24.130369585914497
38	23.391526829512728	27.605002202542682	24.784924885441605	24.218546082502986
39	22.91347410161313	26.341961766252258	25.51262404727876	25.231940084855854
40	23.504159402763165	24.77041577574002	27.831314398197282	23.894110423299537
41	21.941288962381766	26.35811623890969	25.97893486681161	25.721659931896934
42	23.7748963982257	26.809095267263032	25.111454642952495	24.304553691558763
43	23.279268202388618	25.693688761647486	26.82667073520052	24.200372300763373
44	23.56084963551471	25.67454271997942	26.260665878388235	24.50394176611764
45	23.498325843192422	24.616050814791215	26.58465280224009	25.300970539776273
46	23.78970466482835	25.719565833589485	25.80691964870005	24.683809852882117
47	22.8839323576331	26.219606593717256	26.56834875113212	24.32811229751752
48	22.782966903524144	25.703186993256256	26.870198064305274	24.64364803891433
49	23.997244764941208	25.112052956754628	26.709999543785727	24.180702734518444
50	22.201480677081815	24.819253139838647	27.54793802366481	25.43132815941473
51	22.051603069648962	24.92178916767819	25.924413521592772	27.102194241080074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1368.0
1	1651.0
2	1934.0
3	2990.0
4	4046.0
5	3043.5
6	2041.0
7	2135.0
8	2229.0
9	2369.5
10	2510.0
11	2630.5
12	2751.0
13	2675.0
14	2599.0
15	2753.5
16	2908.0
17	2906.0
18	2904.0
19	2954.0
20	3004.0
21	3283.0
22	3562.0
23	3957.0
24	4352.0
25	5850.5
26	8612.0
27	9875.0
28	12459.0
29	15043.0
30	17901.5
31	20760.0
32	22673.5
33	24587.0
34	28110.5
35	31634.0
36	34710.5
37	37787.0
38	41983.0
39	46179.0
40	50160.5
41	54142.0
42	58171.0
43	62200.0
44	69276.0
45	76352.0
46	84803.5
47	93255.0
48	102581.0
49	111907.0
50	119065.5
51	126224.0
52	124543.5
53	122863.0
54	108705.0
55	94547.0
56	84627.5
57	74708.0
58	68663.5
59	62619.0
60	57569.0
61	52519.0
62	47959.0
63	43399.0
64	38307.5
65	33216.0
66	28839.5
67	24463.0
68	22018.0
69	19573.0
70	18018.0
71	16463.0
72	15398.5
73	14334.0
74	11873.0
75	7833.0
76	6254.0
77	5200.0
78	4146.0
79	3287.5
80	2429.0
81	1875.5
82	1322.0
83	985.0
84	648.0
85	462.5
86	277.0
87	245.0
88	213.0
89	142.5
90	72.0
91	75.0
92	78.0
93	49.5
94	21.0
95	15.5
96	10.0
97	6.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1337091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.489415162398117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59646018206655	20.23123648427729
2	9.989264846921671	5.491980970883581
3	3.7155357530253292	3.064137145969403
4	1.9336622474050758	2.1262097721109563
5	1.1923119614331623	1.6387979255464702
6	0.8790747225330542	1.4499150003888635
7	0.6976122065547834	1.3423866097838748
8	0.5323843922037809	1.1707948466616576
9	0.44644304429564313	1.1045212371907043
>10	5.851519283316103	38.3029550692689
>50	1.092260157149203	19.553431321443703
>100	0.06882804156739647	3.2081384372509105
>500	0.003004398635865661	0.5412593314893079
>1k	0.0016387628922903605	0.7742358477344629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2375	0.17762441000649917	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1948	0.14568941081796227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1895	0.14172558187886988	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	1849	0.13828527751663874	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4957845053178878E-4	0.0	0.0	0.1443432047631762	0.0
2	1.4957845053178878E-4	0.0	0.0	0.6001087435335366	0.0
3	1.4957845053178878E-4	0.0	0.0	0.8599265121072537	0.0
4	1.4957845053178878E-4	0.0	0.0	1.187578107997137	0.0
5	1.4957845053178878E-4	0.0	0.0	2.0668002402229915	0.0
6	1.4957845053178878E-4	0.0	0.0	2.9182007806499333	0.0
7	1.4957845053178878E-4	0.0	0.0	3.653304075788409	0.0
8	1.4957845053178878E-4	0.0	0.0	5.026060305543901	0.0
9	1.4957845053178878E-4	0.0	0.0	5.644417620042316	0.0
10	1.4957845053178878E-4	0.0	0.0	6.698272593264034	0.0
11	1.4957845053178878E-4	0.0	0.0	7.857505584885397	0.0
12	1.4957845053178878E-4	0.0	0.0	8.835075548335903	0.0
13	1.4957845053178878E-4	0.0	0.0	9.264141333686338	0.0
14	1.4957845053178878E-4	0.0	0.0	9.453881598185912	0.0
15	2.2436767579768317E-4	0.0	0.0	9.711754846902716	0.0
16	2.2436767579768317E-4	0.0	0.0	10.256070828387896	0.0
17	2.2436767579768317E-4	0.0	0.0	10.896341385889217	0.0
18	2.2436767579768317E-4	0.0	0.0	11.623666601600041	0.0
19	2.2436767579768317E-4	0.0	0.0	12.028725045640124	0.0
20	2.2436767579768317E-4	0.0	0.0	12.458463934017955	0.0
21	2.2436767579768317E-4	0.0	0.0	12.93980738782925	0.0
22	2.2436767579768317E-4	0.0	0.0	13.458470665048228	0.0
23	2.2436767579768317E-4	0.0	0.0	13.98199524190949	0.0
24	2.2436767579768317E-4	0.0	0.0	14.403656893958601	0.0
25	2.2436767579768317E-4	0.0	0.0	14.789569296330617	0.0
26	2.2436767579768317E-4	0.0	0.0	15.138834978322343	0.0
27	2.2436767579768317E-4	0.0	0.0	15.501188774735601	0.0
28	2.9915690106357756E-4	0.0	0.0	15.873339959658692	0.0
29	3.7394612632947195E-4	0.0	0.0	16.264188450898256	0.0
30	3.7394612632947195E-4	0.0	0.0	16.733266471765944	0.0
31	3.7394612632947195E-4	0.0	0.0	17.12905105187306	0.0
32	3.7394612632947195E-4	0.0	0.0	17.525807891908627	0.0
33	3.7394612632947195E-4	0.0	0.0	17.91650680469766	0.0
34	3.7394612632947195E-4	0.0	0.0	18.30743008516249	0.0
35	3.7394612632947195E-4	0.0	0.0	18.70852470026348	0.0
36	3.7394612632947195E-4	0.0	0.0	19.087706072361566	0.0
37	3.7394612632947195E-4	0.0	0.0	19.47421678853571	0.0
38	3.7394612632947195E-4	0.0	0.0	19.855641837391772	0.0
39	3.7394612632947195E-4	0.0	0.0	20.255165878762178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	40	6.8157533E-9	45.000004	1
TATTACG	25	3.8911487E-5	45.0	1
TCATCCG	25	3.8911487E-5	45.0	29
ATCGATC	30	2.1657688E-6	44.999996	13
TACGTCG	30	2.1657688E-6	44.999996	1
TCGTTTA	70	0.0	41.785713	38
CGTAAGG	240	0.0	41.249996	2
TCGATTA	45	1.9286745E-8	40.0	41
TGTTACG	45	1.9286745E-8	40.0	1
CAGTACG	40	3.4587902E-7	39.375004	1
AACACGT	420	0.0	38.571426	41
CGAATAT	100	0.0	38.25	14
CAACGAG	490	0.0	37.65306	14
TACGCAT	60	1.5643309E-10	37.499996	16
ATCGGCC	30	1.14002185E-4	37.499996	33
AACGAGC	500	0.0	36.899998	15
GTCGAAC	55	2.750312E-9	36.81818	10
AGTACGG	190	0.0	36.710526	2
TACGTAG	80	0.0	36.562504	1
GACACGA	450	0.0	36.5	25
>>END_MODULE