FastQCFastQC Report
Sat 18 Jun 2016
SRR3553952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272929
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC110230.8659556031797532No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG90630.7119800083115397No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC79120.6215586258149511No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC45930.36082138123964497No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT28500.22389308437469804No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT21880.17188704161818924No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT20840.1637169080129371No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.15601812826952643No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC18540.1456483433090141No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC17880.1404634508287579No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT17290.1358284711873168No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT15560.12223776817088777No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA12850.10094828541104806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.033574E-445.033
CAACCGT207.033574E-445.041
TGCGAAA207.033574E-445.026
CGGTCGA207.033574E-445.027
CGCAATA253.891044E-544.99999624
CGATGAA7450.039.5637619
CGCATAG403.4586083E-739.37510
TCCCGAT356.24882E-638.57142639
GCGTACG356.24882E-638.5714261
CCGATGA7650.038.23529418
CGTTTTT19150.037.7154051
CGATCGA301.1399907E-437.50000441
GCTACGA850.037.05882610
TCGTTCA552.750312E-936.81818416
AGTACGG1350.036.6666682
TACGGCT12550.036.5737047
TCGAACG800.036.56251
CGGCGAA2300.036.19565231
GACGTAC250.00210719435.99999620
TATTACG250.00210719435.9999961
Produced by FastQC (version 0.11.5)