##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1272929
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8975449534106	33.0	31.0	34.0	30.0	34.0
2	31.98347669037315	33.0	31.0	34.0	30.0	34.0
3	32.01524515507149	33.0	31.0	34.0	30.0	34.0
4	35.6331759273298	37.0	35.0	37.0	33.0	37.0
5	35.50228331666574	37.0	35.0	37.0	33.0	37.0
6	35.4820606648132	37.0	35.0	37.0	33.0	37.0
7	35.762993065599105	37.0	35.0	37.0	35.0	37.0
8	35.52481481685153	37.0	35.0	37.0	33.0	37.0
9	37.25081053224493	39.0	37.0	39.0	34.0	39.0
10	36.79037401143347	39.0	37.0	39.0	32.0	39.0
11	36.78685692603437	39.0	37.0	39.0	32.0	39.0
12	36.76296478436739	39.0	35.0	39.0	33.0	39.0
13	36.732563245868384	39.0	35.0	39.0	32.0	39.0
14	37.846568818842215	40.0	37.0	41.0	33.0	41.0
15	37.93073612118194	40.0	37.0	41.0	33.0	41.0
16	38.032801515245545	40.0	37.0	41.0	33.0	41.0
17	37.92800148319348	40.0	37.0	41.0	33.0	41.0
18	37.90349815268566	40.0	37.0	41.0	33.0	41.0
19	37.85899684899943	40.0	37.0	41.0	33.0	41.0
20	37.719391262199224	40.0	36.0	41.0	33.0	41.0
21	37.684463941036775	39.0	36.0	41.0	33.0	41.0
22	37.68474125422549	39.0	36.0	41.0	33.0	41.0
23	37.60536919184024	39.0	36.0	41.0	33.0	41.0
24	37.60065958117067	39.0	36.0	41.0	33.0	41.0
25	37.53170915267073	39.0	36.0	41.0	32.0	41.0
26	37.47505870319554	39.0	36.0	41.0	32.0	41.0
27	37.39781323231696	39.0	36.0	41.0	32.0	41.0
28	37.28698536996172	39.0	36.0	41.0	32.0	41.0
29	37.269461218968225	39.0	36.0	41.0	32.0	41.0
30	37.15238084763565	39.0	36.0	41.0	31.0	41.0
31	37.07076121291918	39.0	35.0	41.0	31.0	41.0
32	36.93293498694742	39.0	35.0	41.0	31.0	41.0
33	36.826246397088916	39.0	35.0	41.0	31.0	41.0
34	36.732562460278615	39.0	35.0	41.0	31.0	41.0
35	36.59426959398364	39.0	35.0	41.0	30.0	41.0
36	36.53549412418132	39.0	35.0	41.0	30.0	41.0
37	36.46660104373457	39.0	35.0	41.0	30.0	41.0
38	36.40436583658633	39.0	35.0	41.0	30.0	41.0
39	36.36093293498695	39.0	35.0	41.0	30.0	41.0
40	36.19031540643665	39.0	35.0	40.0	29.0	41.0
41	36.074755936898285	39.0	35.0	40.0	29.0	41.0
42	35.95899771314818	38.0	35.0	40.0	28.0	41.0
43	35.90015782498474	38.0	35.0	40.0	28.0	41.0
44	35.83051529189766	38.0	35.0	40.0	28.0	41.0
45	35.79615830890804	38.0	35.0	40.0	28.0	41.0
46	35.66672218167706	38.0	34.0	40.0	28.0	41.0
47	35.47040801175871	38.0	34.0	40.0	27.0	41.0
48	35.4107314704905	38.0	34.0	40.0	27.0	41.0
49	35.32173986137483	38.0	34.0	40.0	27.0	41.0
50	35.16136328106281	37.0	34.0	40.0	27.0	41.0
51	33.601384680528135	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	8.0
11	7.0
12	13.0
13	19.0
14	30.0
15	78.0
16	130.0
17	245.0
18	485.0
19	938.0
20	1699.0
21	3037.0
22	4263.0
23	5644.0
24	7090.0
25	8909.0
26	11118.0
27	13178.0
28	15797.0
29	18883.0
30	24312.0
31	31285.0
32	40665.0
33	53695.0
34	82748.0
35	102668.0
36	111179.0
37	158913.0
38	251502.0
39	324292.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.67086695330219	18.07979863763022	22.22810541672002	13.02122899234757
2	29.21199846967113	28.12662764380417	26.039237066639227	16.622136819885476
3	30.653869933044188	27.922531421626818	25.375963624051302	16.0476350212777
4	26.7023533912732	26.184335497109423	28.84693490367491	18.26637620794247
5	26.652154204987085	30.026183707025293	24.020978389211027	19.300683698776602
6	23.49573306916568	38.36702596924102	24.27071737701003	13.866523584583273
7	79.99527074958618	5.290711422239576	10.474268399887189	4.239749428287045
8	80.71683495308851	6.929058887023549	7.4690732947399265	4.885032865148017
9	74.62427205287962	7.077928148388481	9.456536853194484	8.841262945537418
10	38.66460737401694	25.258989307337643	19.275701944099	16.80070137454642
11	30.043073887074613	26.243490406770526	23.544596752843248	20.168838953311614
12	24.56672760224647	23.02681453561039	29.589552913006145	22.81690494913699
13	24.382978155105274	24.830921441808616	32.154739188124395	18.631361214961714
14	21.892265790157975	29.181831822513278	28.47896465553067	20.44693773179808
15	20.75740280879766	26.163517368211426	33.13476242586979	19.94431739712113
16	22.699145042653594	25.318929806768487	29.00680242181614	22.975122728761775
17	22.847778627087607	26.03428785108989	28.161822065488334	22.95611145633417
18	23.816018018286957	25.317987099044803	28.930285978243873	21.935708904424363
19	23.54821046578403	28.0890764528108	25.877169897142732	22.48554318426244
20	24.13331772628324	27.223670762469865	27.964246238399788	20.67876527284711
21	23.46344532962954	29.203671218111932	27.452198826486	19.88068462577253
22	21.56161105607618	25.17100325312724	27.983493187758313	25.28389250303827
23	21.517853705901903	28.24407331437967	27.591012538798314	22.647060440920114
24	22.453569680634192	26.580429859010202	27.683712131627136	23.282288328728466
25	21.19073412578392	29.75979021610789	26.03562335369844	23.013852304409752
26	21.320984909606114	27.958511433080712	27.610966518949603	23.10953713836357
27	23.676025921320043	27.598475641610804	26.420955135753836	22.304543301315313
28	21.315328663264015	27.476630668324788	28.692723631875776	22.515317036535425
29	24.140859388072705	25.577153164080634	26.689626837003477	23.592360610843183
30	23.512230454330133	29.290400328690758	25.734585353935685	21.462783863043423
31	24.057429754526765	29.057787197871992	23.217555731702237	23.667227315899
32	24.660762697683847	28.75879173151056	23.971564792694643	22.608880778110954
33	24.59854398792077	27.914439846998533	23.227611280754857	24.259404884325832
34	25.085059732318143	27.43884380040049	24.68103091374303	22.795065553538336
35	23.610193498616184	28.691388129267224	24.241650555529805	23.456767816586783
36	25.050886577334637	31.26875104581638	23.337986643402736	20.34237573344625
37	22.091648473716916	30.53453884702132	25.54298000909713	21.830832670164636
38	22.64784603068985	30.511992420629902	24.041325164247183	22.798836384433066
39	24.968321092535405	26.620887732151598	24.97939790828868	23.43139326702432
40	24.344012902526377	27.17127192482849	26.685934565085716	21.798780607559415
41	22.310435224588332	28.830358959533488	25.11593341026876	23.743272405609424
42	22.008847311986766	28.832951405773617	26.837553390644725	22.3206478915949
43	22.87393876641981	27.57710759987399	26.216466118691617	23.332487515014584
44	23.178747597077294	26.361721667115763	26.246239970964602	24.213290764842345
45	23.04119082839656	25.421920625580846	26.004356880862957	25.53253166515964
46	25.44281731345582	27.122408241150918	25.0057937245518	22.42898072084146
47	22.005155040069006	26.72482125868764	28.764447977852655	22.5055757233907
48	22.332667415071857	27.38118151130189	26.053534800448414	24.232616273177847
49	22.23462581180883	25.51469877738664	28.82588109784599	23.42479431295854
50	22.12425044916095	25.128895641469402	27.920331770271556	24.826522139098095
51	22.29220954193046	24.888819407838145	26.00804915278071	26.810921897450683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	917.0
1	1172.0
2	1427.0
3	2686.5
4	3946.0
5	2920.5
6	1895.0
7	1889.0
8	1883.0
9	1946.0
10	2009.0
11	2098.0
12	2187.0
13	2249.5
14	2312.0
15	2387.5
16	2463.0
17	2495.0
18	2527.0
19	2634.5
20	2742.0
21	3218.5
22	3695.0
23	4044.0
24	4393.0
25	5477.5
26	8194.0
27	9826.0
28	11840.5
29	13855.0
30	16867.5
31	19880.0
32	22404.0
33	24928.0
34	28770.0
35	32612.0
36	34411.5
37	36211.0
38	40038.0
39	43865.0
40	49696.0
41	55527.0
42	61913.0
43	68299.0
44	77533.0
45	86767.0
46	116529.0
47	146291.0
48	139760.5
49	133230.0
50	128732.5
51	124235.0
52	110072.0
53	95909.0
54	84825.5
55	73742.0
56	67430.0
57	61118.0
58	54780.5
59	48443.0
60	44947.0
61	41451.0
62	36961.0
63	32471.0
64	28632.0
65	24793.0
66	21351.5
67	17910.0
68	15361.0
69	12812.0
70	11680.0
71	10548.0
72	8860.0
73	7172.0
74	5879.0
75	3794.0
76	3002.0
77	2522.0
78	2042.0
79	1606.0
80	1170.0
81	917.0
82	664.0
83	482.0
84	300.0
85	204.0
86	108.0
87	90.5
88	73.0
89	66.5
90	60.0
91	46.0
92	32.0
93	27.5
94	23.0
95	15.0
96	7.0
97	7.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1272929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.86972135140533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79083816691487	22.779026124993077
2	10.116212476789078	6.245693205801777
3	3.846403790285767	3.5621223963333284
4	1.961388525215158	2.4219006894094313
5	1.2463834747223637	1.9237755280837863
6	0.9050760935006721	1.6763668084910537
7	0.7105226123042699	1.535354453899379
8	0.6183046083114792	1.5269512775092144
9	0.47734418541622875	1.326193379326129
>10	5.760180406153508	39.61928896050999
>50	0.5123781102183457	9.891849146629728
>100	0.04883163760772033	2.9531207219053126
>500	0.0025566302335674074	0.5003684148418186
>1k	0.0028122932569241478	1.828317775745358
>5k	5.113260467134814E-4	1.3397088078769512
>10k+	2.556630233567407E-4	0.8699623086437488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	11023	0.8659556031797532	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	9063	0.7119800083115397	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	7912	0.6215586258149511	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	4593	0.36082138123964497	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	2850	0.22389308437469804	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	2188	0.17188704161818924	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT	2084	0.1637169080129371	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1986	0.15601812826952643	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC	1854	0.1456483433090141	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1788	0.1404634508287579	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1729	0.1358284711873168	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT	1556	0.12223776817088777	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA	1285	0.10094828541104806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14423428172348968	0.0
2	0.0	0.0	0.0	0.8079005191962788	0.0
3	0.0	0.0	0.0	1.0865492105215608	0.0
4	0.0	0.0	0.0	1.6845401432444387	0.0
5	0.0	0.0	0.0	3.600672150606986	0.0
6	0.0	0.0	0.0	4.686907125220652	0.0
7	0.0	0.0	0.0	5.621601833252287	0.0
8	0.0	0.0	0.0	7.036527567523405	0.0
9	0.0	0.0	0.0	7.610715130223288	0.0
10	0.0	0.0	0.0	9.44813104265831	0.0
11	0.0	0.0	0.0	10.665009595979038	0.0
12	0.0	0.0	0.0	12.373745904131338	0.0
13	0.0	0.0	0.0	12.885243403206307	0.0
14	0.0	0.0	0.0	13.119034918679676	0.0
15	0.0	0.0	0.0	13.520078496129791	0.0
16	0.0	0.0	0.0	14.187751241428234	0.0
17	0.0	0.0	0.0	15.000051063335032	0.0
18	0.0	0.0	0.0	15.905443272955523	0.0
19	0.0	0.0	0.0	16.59566244464538	0.0
20	0.0	0.0	0.0	17.142432924381485	0.0
21	0.0	0.0	0.0	17.739638267334627	0.0
22	7.855897697357827E-5	0.0	0.0	18.425222459383043	0.0
23	7.855897697357827E-5	0.0	0.0	19.070820132151912	0.0
24	7.855897697357827E-5	0.0	0.0	19.576347148976886	0.0
25	7.855897697357827E-5	0.0	0.0	20.050293457058483	0.0
26	7.855897697357827E-5	0.0	0.0	20.487788399824343	0.0
27	7.855897697357827E-5	0.0	0.0	20.937538542998077	0.0
28	7.855897697357827E-5	0.0	0.0	21.394673230007328	0.0
29	7.855897697357827E-5	0.0	0.0	21.860920758345518	0.0
30	7.855897697357827E-5	0.0	0.0	22.406512853427017	0.0
31	7.855897697357827E-5	0.0	0.0	22.914553757515147	0.0
32	1.5711795394715653E-4	0.0	0.0	23.393763517053976	0.0
33	1.5711795394715653E-4	0.0	0.0	23.871087861145437	0.0
34	2.3567693092073477E-4	0.0	0.0	24.325865778845483	0.0
35	2.3567693092073477E-4	0.0	0.0	24.803190122936943	0.0
36	2.3567693092073477E-4	0.0	0.0	25.239978034910038	0.0
37	3.927948848678913E-4	0.0	0.0	25.69664136805745	0.0
38	3.927948848678913E-4	0.0	0.0	26.16579557854366	0.0
39	3.927948848678913E-4	0.0	0.0	26.622301793737122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.033574E-4	45.0	33
CAACCGT	20	7.033574E-4	45.0	41
TGCGAAA	20	7.033574E-4	45.0	26
CGGTCGA	20	7.033574E-4	45.0	27
CGCAATA	25	3.891044E-5	44.999996	24
CGATGAA	745	0.0	39.56376	19
CGCATAG	40	3.4586083E-7	39.375	10
TCCCGAT	35	6.24882E-6	38.571426	39
GCGTACG	35	6.24882E-6	38.571426	1
CCGATGA	765	0.0	38.235294	18
CGTTTTT	1915	0.0	37.715405	1
CGATCGA	30	1.1399907E-4	37.500004	41
GCTACGA	85	0.0	37.058826	10
TCGTTCA	55	2.750312E-9	36.818184	16
AGTACGG	135	0.0	36.666668	2
TACGGCT	1255	0.0	36.573704	7
TCGAACG	80	0.0	36.5625	1
CGGCGAA	230	0.0	36.195652	31
GACGTAC	25	0.002107194	35.999996	20
TATTACG	25	0.002107194	35.999996	1
>>END_MODULE