FastQCFastQC Report
Sat 18 Jun 2016
SRR3553951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845344
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC28430.3363127910057917No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC18690.22109342468864748No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT16740.19802589241776128No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC12370.14633096112351895No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC10630.12574762463565128No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA9410.1113156300866866No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC253.8899263E-545.00000433
GTCGAAT207.032225E-445.00000422
TTAGCGA207.032225E-445.0000041
TCTAGCG253.8899263E-545.0000041
CGCTCGA253.8899263E-545.00000443
TGCGACG502.1827873E-1145.0000041
ACGCTAT302.1648175E-645.00000440
GTATACG253.8899263E-545.0000041
ACGAGGT207.032225E-445.0000042
TACGTAG253.8899263E-545.0000041
TATCGTG551.8189894E-1245.01
CACGACC1750.042.4285727
AACACGT1700.042.3529441
ACGCATT1500.042.017
TACGCAT1500.042.016
ACGTTTA501.0804797E-940.50000432
ACGACCA1850.040.13513628
ACACGAC1800.040.026
ATAACGC451.9272193E-840.011
TCGTTTA403.456771E-739.37500438