##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845344
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98709282848166	33.0	31.0	34.0	30.0	34.0
2	32.09542505772798	33.0	31.0	34.0	30.0	34.0
3	32.12084784419124	34.0	31.0	34.0	30.0	34.0
4	35.72087812772079	37.0	35.0	37.0	33.0	37.0
5	35.58729937161676	37.0	35.0	37.0	33.0	37.0
6	35.57718396297839	37.0	35.0	37.0	33.0	37.0
7	35.808175133436805	37.0	35.0	37.0	35.0	37.0
8	35.666108708407464	37.0	35.0	37.0	35.0	37.0
9	37.33520436650642	39.0	37.0	39.0	34.0	39.0
10	36.94530037475868	39.0	37.0	39.0	33.0	39.0
11	36.892146865654695	39.0	37.0	39.0	33.0	39.0
12	36.76944060642768	39.0	35.0	39.0	33.0	39.0
13	36.7092982265208	39.0	35.0	39.0	33.0	39.0
14	37.774684625430595	40.0	36.0	41.0	33.0	41.0
15	37.92079200893364	40.0	37.0	41.0	33.0	41.0
16	37.98343159707764	40.0	37.0	41.0	33.0	41.0
17	37.95284050043533	40.0	36.0	41.0	33.0	41.0
18	37.91977467161298	40.0	36.0	41.0	33.0	41.0
19	37.871458246583636	40.0	36.0	41.0	33.0	41.0
20	37.80256794866942	40.0	36.0	41.0	33.0	41.0
21	37.70717601355188	40.0	36.0	41.0	33.0	41.0
22	37.700822387099215	40.0	36.0	41.0	33.0	41.0
23	37.621312743687774	39.0	36.0	41.0	33.0	41.0
24	37.590598620206684	39.0	36.0	41.0	33.0	41.0
25	37.48846741681493	39.0	35.0	41.0	33.0	41.0
26	37.42837353787334	39.0	35.0	41.0	33.0	41.0
27	37.37832409054775	39.0	35.0	41.0	32.0	41.0
28	37.28481541242382	39.0	35.0	41.0	32.0	41.0
29	37.25161709315971	39.0	35.0	41.0	32.0	41.0
30	37.12567783056365	39.0	35.0	41.0	32.0	41.0
31	37.053895218987776	39.0	35.0	41.0	31.0	41.0
32	36.93614552182307	39.0	35.0	41.0	31.0	41.0
33	36.86163502668736	39.0	35.0	41.0	31.0	41.0
34	36.7841754362721	39.0	35.0	41.0	31.0	41.0
35	36.71837027293031	39.0	35.0	41.0	31.0	41.0
36	36.598999933754776	39.0	35.0	41.0	31.0	41.0
37	36.54157242495363	39.0	35.0	41.0	30.0	41.0
38	36.46725829957982	39.0	35.0	40.0	30.0	41.0
39	36.39222375742893	39.0	35.0	40.0	30.0	41.0
40	36.24979180073438	38.0	35.0	40.0	30.0	41.0
41	36.10997889616535	38.0	35.0	40.0	30.0	41.0
42	36.02740304538744	38.0	35.0	40.0	30.0	41.0
43	35.96598071317712	38.0	35.0	40.0	30.0	41.0
44	35.90097522428739	38.0	35.0	40.0	29.0	41.0
45	35.826119307642806	38.0	35.0	40.0	29.0	41.0
46	35.6923914997918	38.0	34.0	40.0	29.0	41.0
47	35.58900400310406	38.0	34.0	40.0	28.0	41.0
48	35.46378515728508	38.0	34.0	40.0	28.0	41.0
49	35.39885419426884	37.0	34.0	40.0	28.0	41.0
50	35.276630578793956	37.0	34.0	40.0	27.0	41.0
51	33.73825685164856	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	7.0
12	8.0
13	14.0
14	22.0
15	42.0
16	87.0
17	141.0
18	312.0
19	578.0
20	957.0
21	1441.0
22	2080.0
23	2920.0
24	3835.0
25	5043.0
26	6511.0
27	8213.0
28	10245.0
29	12625.0
30	16087.0
31	20260.0
32	26476.0
33	36846.0
34	59880.0
35	74993.0
36	73128.0
37	105040.0
38	167093.0
39	210396.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08016144906689	18.700552674414205	23.06268217435742	14.156603702161489
2	29.66934171177651	25.31147083317561	26.581368058447215	18.437819396600673
3	32.16028031192035	24.565384033009046	26.25440057538706	17.01993507968354
4	29.44126887988795	26.937199530605294	24.164955331793923	19.456576257712836
5	24.473232198962787	30.941723132831132	24.114443350872545	20.470601317333536
6	24.596022447666275	36.21283169928455	24.06180205928001	15.129343793769165
7	81.1908524813567	6.230244728773139	7.587798576674111	4.991104213196048
8	82.5376414808646	4.958573077942234	6.918485255706552	5.585300185486618
9	76.44450070030662	7.524629026763069	9.801571904455464	6.229298368474845
10	41.080672483627964	24.939906121058407	16.824748268160654	17.154673127152968
11	31.815331983192642	26.653528031192035	22.11407427035621	19.417065715259113
12	28.12914032630503	22.78338759132377	28.411628875345425	20.67584320702578
13	24.788961653480712	25.085290721883634	29.709325434379373	20.416422190256274
14	21.425952038460082	28.161908051633418	28.4647433470871	21.947396562819396
15	19.557245334443728	26.68925313245259	32.43247719271681	21.32102434038687
16	21.92598516107052	24.083804936215316	31.55236211530454	22.437847787409623
17	21.646808873074157	24.860411856001818	27.939040201385474	25.553739069538555
18	23.513859446568496	24.83568819320892	28.444041715561948	23.206410644660636
19	24.68687303630238	26.812043381156077	25.45685543400083	23.044228148540714
20	25.412731385092936	26.082872771321497	25.504173448915473	23.0002223946701
21	24.394802589241777	27.682813150622703	26.48105386682818	21.44133039330734
22	22.924750160881253	24.872596244842338	25.630985728886703	26.57166786538971
23	22.250823333459515	27.556355755763335	25.447983306204335	24.744837604572815
24	22.77806431464587	25.849594957792334	27.646614869213003	23.725725858348788
25	22.931611273043874	26.279124238179957	26.09848771624333	24.69077677253284
26	21.200718287466405	29.040840178672823	25.555868380209713	24.20257315365106
27	23.192570125298104	26.93069330355453	25.668366960669264	24.2083696104781
28	20.8645237914979	26.61508214407389	27.636559791043645	24.883834273384565
29	22.000984214710225	26.68085418480524	26.59674641329447	24.72141518719007
30	23.514450921754932	25.652397130635574	26.264810538668282	24.56834140894121
31	24.385102396184273	26.123684559185374	23.722650187379337	25.768562857251016
32	23.853011318469168	28.73078888594466	23.02104232123254	24.395157474353635
33	23.633574024302533	26.316505469962525	23.13223965628194	26.917680849453003
34	21.62066566983382	26.882547223378882	25.796480486050648	25.700306620736647
35	22.204451678843167	26.0395767876746	24.876026800923647	26.87994473255858
36	23.039969527198394	28.727121739788775	24.291531021690577	23.941377711322254
37	21.75717814286255	27.459472120225612	26.484602717946775	24.29874701896506
38	22.22527160540561	28.903972820532232	24.091257523564373	24.779498050497786
39	23.636294810160123	25.789146193738883	25.82853844115532	24.74602055494568
40	23.73755536207745	26.112683120717723	27.604383540901694	22.545377976303136
41	20.72623689290987	27.259435212173976	26.404990157852897	25.609337737063253
42	21.31629253889541	27.80536680925162	25.888750615134192	24.989590036718777
43	22.7645786803952	25.051221751145096	26.400968126585155	25.78323144187455
44	22.184459817541736	25.63417969489344	25.379608774652684	26.801751712912143
45	22.439267327857063	24.792747094673885	26.2478943483363	26.520091229132753
46	23.62221770072302	25.88910550024605	25.86745750842261	24.62121929060832
47	22.023578566831965	25.683272135367375	27.24725082333346	25.045898474467197
48	21.52709429534012	25.821204148843545	26.46378279138434	26.187918764431995
49	22.403069046447364	24.44093765378355	28.355911912783434	24.800081386985653
50	21.521297838513078	24.110539614642086	28.461549381080363	25.90661316576447
51	21.443932884127644	24.68438884051936	25.839421584585683	28.03225669076731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1080.0
1	1278.5
2	1477.0
3	1726.0
4	1975.0
5	1532.0
6	1089.0
7	1115.0
8	1141.0
9	1221.0
10	1301.0
11	1382.0
12	1463.0
13	1496.5
14	1530.0
15	1550.5
16	1571.0
17	1680.5
18	1790.0
19	1728.0
20	1666.0
21	1981.0
22	2296.0
23	2559.5
24	2823.0
25	3853.0
26	5737.5
27	6592.0
28	7996.0
29	9400.0
30	10879.0
31	12358.0
32	13130.5
33	13903.0
34	16163.5
35	18424.0
36	19373.5
37	20323.0
38	22156.5
39	23990.0
40	27737.5
41	31485.0
42	36859.5
43	42234.0
44	48680.5
45	55127.0
46	62585.5
47	70044.0
48	78022.5
49	86001.0
50	83934.0
51	81867.0
52	72172.5
53	62478.0
54	56551.5
55	50625.0
56	47974.5
57	45324.0
58	42377.0
59	39430.0
60	37717.0
61	36004.0
62	33907.0
63	31810.0
64	27334.0
65	22858.0
66	19533.5
67	16209.0
68	14369.5
69	12530.0
70	10978.0
71	9426.0
72	8367.5
73	7309.0
74	6571.0
75	4744.0
76	3655.0
77	2744.0
78	1833.0
79	1490.0
80	1147.0
81	836.0
82	525.0
83	408.0
84	291.0
85	193.0
86	95.0
87	79.0
88	63.0
89	47.0
90	31.0
91	24.5
92	18.0
93	12.0
94	6.0
95	4.5
96	3.0
97	4.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	845344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.300162811848338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95063023610808	19.915640826726964
2	10.563377095174383	5.7676382908242045
3	3.9392654656653314	3.226277657152653
4	2.0087259435714335	2.1935418121553525
5	1.1960520197435898	1.6326207435220021
6	0.7994520054145307	1.3095101944845204
7	0.6385478198581782	1.2202721611695349
8	0.5125538210932731	1.119424221254507
9	0.43169756451860286	1.060687241715263
>10	5.835491357062987	39.06206077184212
>50	1.044309403537283	17.893614502420476
>100	0.07077855828879341	3.3363005487212503
>500	0.006947588543685243	1.232738395613711
>1k	0.0021711214199016384	1.0296726323974124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	2843	0.3363127910057917	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1869	0.22109342468864748	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	1674	0.19802589241776128	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1237	0.14633096112351895	No Hit
TCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1063	0.12574762463565128	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	941	0.1113156300866866	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1829503728659576E-4	0.0	0.0	0.20429552939395088	0.0
2	1.1829503728659576E-4	0.0	0.0	1.0017223757428928	0.0
3	1.1829503728659576E-4	0.0	0.0	1.360984403982284	0.0
4	1.1829503728659576E-4	0.0	0.0	1.8328633077185146	0.0
5	1.1829503728659576E-4	0.0	0.0	3.1318611121626225	0.0
6	1.1829503728659576E-4	0.0	0.0	4.216035128894273	0.0
7	1.1829503728659576E-4	0.0	0.0	5.122766589696029	0.0
8	1.1829503728659576E-4	0.0	0.0	6.625941628496801	0.0
9	1.1829503728659576E-4	0.0	0.0	7.264143354657985	0.0
10	1.1829503728659576E-4	0.0	0.0	8.452890184350986	0.0
11	1.1829503728659576E-4	0.0	0.0	9.831382253851686	0.0
12	1.1829503728659576E-4	0.0	0.0	10.886100806298975	0.0
13	1.1829503728659576E-4	0.0	0.0	11.323319264110232	0.0
14	1.1829503728659576E-4	0.0	0.0	11.497804444107961	0.0
15	1.1829503728659576E-4	0.0	0.0	11.786444335087255	0.0
16	1.1829503728659576E-4	0.0	0.0	12.443454972177006	0.0
17	1.1829503728659576E-4	0.0	0.0	13.213319074838173	0.0
18	1.1829503728659576E-4	0.0	0.0	14.174111367679902	0.0
19	1.1829503728659576E-4	0.0	0.0	14.631321686792596	0.0
20	1.1829503728659576E-4	0.0	0.0	15.096576068440777	0.0
21	1.1829503728659576E-4	0.0	0.0	15.672081424840066	0.0
22	1.1829503728659576E-4	0.0	0.0	16.257996744520575	0.0
23	1.1829503728659576E-4	0.0	0.0	16.858107468675474	0.0
24	1.1829503728659576E-4	0.0	0.0	17.324544800696522	0.0
25	2.3659007457319152E-4	0.0	0.0	17.72698451754552	0.0
26	2.3659007457319152E-4	0.0	0.0	18.114045879547263	0.0
27	2.3659007457319152E-4	0.0	0.0	18.502999962145587	0.0
28	2.3659007457319152E-4	0.0	0.0	18.905557974031872	0.0
29	2.3659007457319152E-4	0.0	0.0	19.31296608244691	0.0
30	2.3659007457319152E-4	0.0	0.0	19.772069122156186	0.0
31	3.5488511185978726E-4	0.0	0.0	20.20431918840141	0.0
32	3.5488511185978726E-4	0.0	0.0	20.618233523867207	0.0
33	3.5488511185978726E-4	0.0	0.0	21.02268425635008	0.0
34	3.5488511185978726E-4	0.0	0.0	21.427726464019383	0.0
35	3.5488511185978726E-4	0.0	0.0	21.87665613052201	0.0
36	3.5488511185978726E-4	0.0	0.0	22.30027065904531	0.0
37	3.5488511185978726E-4	0.0	0.0	22.709453193019648	0.0
38	3.5488511185978726E-4	0.0	0.0	23.086104591740167	0.0
39	3.5488511185978726E-4	0.0	0.0	23.476714804860507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCC	25	3.8899263E-5	45.000004	33
GTCGAAT	20	7.032225E-4	45.000004	22
TTAGCGA	20	7.032225E-4	45.000004	1
TCTAGCG	25	3.8899263E-5	45.000004	1
CGCTCGA	25	3.8899263E-5	45.000004	43
TGCGACG	50	2.1827873E-11	45.000004	1
ACGCTAT	30	2.1648175E-6	45.000004	40
GTATACG	25	3.8899263E-5	45.000004	1
ACGAGGT	20	7.032225E-4	45.000004	2
TACGTAG	25	3.8899263E-5	45.000004	1
TATCGTG	55	1.8189894E-12	45.0	1
CACGACC	175	0.0	42.42857	27
AACACGT	170	0.0	42.35294	41
ACGCATT	150	0.0	42.0	17
TACGCAT	150	0.0	42.0	16
ACGTTTA	50	1.0804797E-9	40.500004	32
ACGACCA	185	0.0	40.135136	28
ACACGAC	180	0.0	40.0	26
ATAACGC	45	1.9272193E-8	40.0	11
TCGTTTA	40	3.456771E-7	39.375004	38
>>END_MODULE